This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGACGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 166.23 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 283,442 (8.2%) Reads that were too short: 85,866 (2.5%) Reads written (passing filters): 197,576 (5.7%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 55,347,051 bp (5.3%) === Adapter 1 === Sequence: CGACGT; Type: regular 5'; Length: 6; Trimmed: 283442 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3575 847.3 0 3575 7 78742 847.3 0 78742 8 4192 847.3 0 4192 9 89896 847.3 0 89896 10 101 847.3 0 101 11 37 847.3 0 37 12 36 847.3 0 36 13 30 847.3 0 30 14 35 847.3 0 35 15 32 847.3 0 32 16 98 847.3 0 98 17 199 847.3 0 199 18 214 847.3 0 214 19 36 847.3 0 36 20 33 847.3 0 33 21 39 847.3 0 39 22 38 847.3 0 38 23 31 847.3 0 31 24 28 847.3 0 28 25 84 847.3 0 84 26 33 847.3 0 33 27 29 847.3 0 29 28 21 847.3 0 21 29 31 847.3 0 31 30 37 847.3 0 37 31 33 847.3 0 33 32 34 847.3 0 34 33 32 847.3 0 32 34 32 847.3 0 32 35 32 847.3 0 32 36 24 847.3 0 24 37 39 847.3 0 39 38 43 847.3 0 43 39 21 847.3 0 21 40 24 847.3 0 24 41 26 847.3 0 26 42 32 847.3 0 32 43 33 847.3 0 33 44 42 847.3 0 42 45 30 847.3 0 30 46 26 847.3 0 26 47 39 847.3 0 39 48 43 847.3 0 43 49 37 847.3 0 37 50 120 847.3 0 120 51 247 847.3 0 247 52 46 847.3 0 46 53 54 847.3 0 54 54 61 847.3 0 61 55 50 847.3 0 50 56 55 847.3 0 55 57 50 847.3 0 50 58 29 847.3 0 29 59 41 847.3 0 41 60 37 847.3 0 37 61 65 847.3 0 65 62 57 847.3 0 57 63 53 847.3 0 53 64 43 847.3 0 43 65 60 847.3 0 60 66 107 847.3 0 107 67 1500 847.3 0 1500 68 788 847.3 0 788 69 234 847.3 0 234 70 41 847.3 0 41 71 63 847.3 0 63 72 59 847.3 0 59 73 37 847.3 0 37 74 64 847.3 0 64 75 28 847.3 0 28 76 39 847.3 0 39 77 30 847.3 0 30 78 33 847.3 0 33 79 29 847.3 0 29 80 31 847.3 0 31 81 64 847.3 0 64 82 32 847.3 0 32 83 61 847.3 0 61 84 82 847.3 0 82 85 87 847.3 0 87 86 102 847.3 0 102 87 101 847.3 0 101 88 44 847.3 0 44 89 39 847.3 0 39 90 223 847.3 0 223 91 58 847.3 0 58 92 42 847.3 0 42 93 61 847.3 0 61 94 93 847.3 0 93 95 53 847.3 0 53 96 85 847.3 0 85 97 46 847.3 0 46 98 74 847.3 0 74 99 88 847.3 0 88 100 61 847.3 0 61 101 24 847.3 0 24 102 34 847.3 0 34 103 26 847.3 0 26 104 30 847.3 0 30 105 38 847.3 0 38 106 38 847.3 0 38 107 42 847.3 0 42 108 33 847.3 0 33 109 28 847.3 0 28 110 31 847.3 0 31 111 40 847.3 0 40 112 37 847.3 0 37 113 44 847.3 0 44 114 44 847.3 0 44 115 33 847.3 0 33 116 34 847.3 0 34 117 39 847.3 0 39 118 91 847.3 0 91 119 100 847.3 0 100 120 107 847.3 0 107 121 127 847.3 0 127 122 119 847.3 0 119 123 82 847.3 0 82 124 27 847.3 0 27 125 39 847.3 0 39 126 70 847.3 0 70 127 156 847.3 0 156 128 83 847.3 0 83 129 170 847.3 0 170 130 69 847.3 0 69 131 55 847.3 0 55 132 150 847.3 0 150 133 362 847.3 0 362 134 69 847.3 0 69 135 44 847.3 0 44 136 32 847.3 0 32 137 42 847.3 0 42 138 42 847.3 0 42 139 56 847.3 0 56 140 41 847.3 0 41 141 49 847.3 0 49 142 41 847.3 0 41 143 44 847.3 0 44 144 51 847.3 0 51 145 60 847.3 0 60 146 74 847.3 0 74 147 90 847.3 0 90 148 85 847.3 0 85 149 77 847.3 0 77 150 73 847.3 0 73 151 78 847.3 0 78 152 446 847.3 0 446 153 493 847.3 0 493 154 211 847.3 0 211 155 114 847.3 0 114 156 56 847.3 0 56 157 70 847.3 0 70 158 133 847.3 0 133 159 257 847.3 0 257 160 79 847.3 0 79 161 73 847.3 0 73 162 82 847.3 0 82 163 297 847.3 0 297 164 514 847.3 0 514 165 623 847.3 0 623 166 354 847.3 0 354 167 135 847.3 0 135 168 57 847.3 0 57 169 62 847.3 0 62 170 54 847.3 0 54 171 40 847.3 0 40 172 56 847.3 0 56 173 120 847.3 0 120 174 112 847.3 0 112 175 95 847.3 0 95 176 85 847.3 0 85 177 82 847.3 0 82 178 125 847.3 0 125 179 145 847.3 0 145 180 238 847.3 0 238 181 145 847.3 0 145 182 121 847.3 0 121 183 463 847.3 0 463 184 471 847.3 0 471 185 358 847.3 0 358 186 322 847.3 0 322 187 178 847.3 0 178 188 95 847.3 0 95 189 112 847.3 0 112 190 73 847.3 0 73 191 95 847.3 0 95 192 304 847.3 0 304 193 364 847.3 0 364 194 357 847.3 0 357 195 550 847.3 0 550 196 69 847.3 0 69 197 58 847.3 0 58 198 218 847.3 0 218 199 278 847.3 0 278 200 175 847.3 0 175 201 216 847.3 0 216 202 358 847.3 0 358 203 154 847.3 0 154 204 146 847.3 0 146 205 197 847.3 0 197 206 235 847.3 0 235 207 370 847.3 0 370 208 246 847.3 0 246 209 224 847.3 0 224 210 102 847.3 0 102 211 115 847.3 0 115 212 227 847.3 0 227 213 225 847.3 0 225 214 435 847.3 0 435 215 529 847.3 0 529 216 617 847.3 0 617 217 1107 847.3 0 1107 218 1317 847.3 0 1317 219 8812 847.3 0 8812 220 13891 847.3 0 13891 221 40497 847.3 0 40497 222 643 847.3 0 643 223 71 847.3 0 71 224 80 847.3 0 80 225 64 847.3 0 64 226 55 847.3 0 55 227 64 847.3 0 64 228 80 847.3 0 80 229 67 847.3 0 67 230 106 847.3 0 106 231 76 847.3 0 76 232 71 847.3 0 71 233 90 847.3 0 90 234 121 847.3 0 121 235 123 847.3 0 123 236 123 847.3 0 123 237 176 847.3 0 176 238 147 847.3 0 147 239 135 847.3 0 135 240 142 847.3 0 142 241 95 847.3 0 95 242 72 847.3 0 72 243 94 847.3 0 94 244 86 847.3 0 86 245 72 847.3 0 72 246 114 847.3 0 114 247 193 847.3 0 193 248 696 847.3 0 696 249 997 847.3 0 997 250 797 847.3 0 797 251 370 847.3 0 370 252 229 847.3 0 229 253 204 847.3 0 204 254 146 847.3 0 146 255 107 847.3 0 107 256 105 847.3 0 105 257 101 847.3 0 101 258 99 847.3 0 99 259 81 847.3 0 81 260 89 847.3 0 89 261 51 847.3 0 51 262 91 847.3 0 91 263 62 847.3 0 62 264 58 847.3 0 58 265 82 847.3 0 82 266 117 847.3 0 117 267 153 847.3 0 153 268 123 847.3 0 123 269 118 847.3 0 118 270 108 847.3 0 108 271 109 847.3 0 109 272 203 847.3 0 203 273 242 847.3 0 242 274 126 847.3 0 126 275 108 847.3 0 108 276 90 847.3 0 90 277 108 847.3 0 108 278 178 847.3 0 178 279 632 847.3 0 632 280 1053 847.3 0 1053 281 1298 847.3 0 1298 282 625 847.3 0 625 283 130 847.3 0 130 284 186 847.3 0 186 285 129 847.3 0 129 286 120 847.3 0 120 287 157 847.3 0 157 288 151 847.3 0 151 289 112 847.3 0 112 290 103 847.3 0 103 291 104 847.3 0 104 292 242 847.3 0 242 293 251 847.3 0 251 294 265 847.3 0 265 295 156 847.3 0 156 296 95 847.3 0 95 297 144 847.3 0 144 298 123 847.3 0 123 299 175 847.3 0 175 300 263 847.3 0 263 301 240 847.3 0 240 Finished in 15.58 s (79 us/read; 0.76 M reads/minute). === Summary === Total reads processed: 197,576 Reads with adapters: 81,892 (41.4%) Reads that were too short: 51 (0.0%) Reads written (passing filters): 81,841 (41.4%) Total basepairs processed: 55,347,051 bp Total written (filtered): 22,332,735 bp (40.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 81892 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 36 0.0 3 0 0 30 3 3 19 1264 0.0 3 0 1153 78 27 6 20 79394 0.0 4 76391 2533 349 93 28 21 149 0.0 4 27 114 3 1 4 22 24 0.0 4 3 3 11 2 5 23 96 0.0 4 0 0 0 77 19 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 0 0 1 26 1 0.0 4 1 32 1 0.0 4 1 39 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 44 2 0.0 4 0 0 0 1 1 49 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 68 1 0.0 4 1 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 94 7 0.0 4 0 0 0 0 7 103 3 0.0 4 0 0 0 0 3 104 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 2 0.0 4 0 0 0 0 2 108 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 116 2 0.0 4 1 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 841 0.0 4 0 0 0 0 841 128 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 132 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 149 2 0.0 4 1 0 0 0 1 153 3 0.0 4 0 0 0 0 3 162 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 1 4 229 5 0.0 4 0 1 0 0 4 234 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 264 3 0.0 4 0 0 0 0 3 265 3 0.0 4 0 0 0 0 3 268 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 292 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.51 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 81,841 Reads with adapters: 6,657 (8.1%) Reads written (passing filters): 81,841 (100.0%) Total basepairs processed: 22,332,735 bp Total written (filtered): 22,240,013 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6657 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.4% C: 8.2% G: 85.5% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 213 1278.8 0 213 4 34 319.7 0 34 5 1 79.9 0 1 6 1 20.0 0 1 7 3 5.0 0 3 9 25 0.3 0 0 25 10 70 0.1 1 4 66 11 5622 0.0 1 152 5470 12 543 0.0 1 9 534 13 19 0.0 1 2 17 14 7 0.0 1 0 7 15 5 0.0 1 0 5 20 2 0.0 1 2 24 1 0.0 1 1 123 1 0.0 1 1 152 19 0.0 1 14 5 153 2 0.0 1 2 158 1 0.0 1 1 191 1 0.0 1 0 1 210 74 0.0 1 58 16 211 9 0.0 1 7 2 226 1 0.0 1 1 271 1 0.0 1 1 273 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.