This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAGAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 172.06 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 552,215 (15.9%) Reads that were too short: 16,661 (0.5%) Reads written (passing filters): 535,554 (15.4%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 128,937,937 bp (12.3%) === Adapter 1 === Sequence: AGAGAC; Type: regular 5'; Length: 6; Trimmed: 552215 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2573 847.3 0 2573 7 91662 847.3 0 91662 8 66935 847.3 0 66935 9 109 847.3 0 109 10 183 847.3 0 183 11 86 847.3 0 86 12 21 847.3 0 21 13 64 847.3 0 64 14 17 847.3 0 17 15 15 847.3 0 15 16 10 847.3 0 10 17 9 847.3 0 9 18 9 847.3 0 9 19 12 847.3 0 12 20 19 847.3 0 19 21 23 847.3 0 23 22 16 847.3 0 16 23 19 847.3 0 19 24 5 847.3 0 5 25 15 847.3 0 15 26 7 847.3 0 7 27 42 847.3 0 42 28 29 847.3 0 29 29 37 847.3 0 37 30 23 847.3 0 23 31 20 847.3 0 20 32 13 847.3 0 13 33 16 847.3 0 16 34 21 847.3 0 21 35 27 847.3 0 27 36 29 847.3 0 29 37 13 847.3 0 13 38 14 847.3 0 14 39 52 847.3 0 52 40 31 847.3 0 31 41 27 847.3 0 27 42 79 847.3 0 79 43 104 847.3 0 104 44 66 847.3 0 66 45 148 847.3 0 148 46 199 847.3 0 199 47 95 847.3 0 95 48 67 847.3 0 67 49 226 847.3 0 226 50 57 847.3 0 57 51 104 847.3 0 104 52 936 847.3 0 936 53 244 847.3 0 244 54 88 847.3 0 88 55 88 847.3 0 88 56 40 847.3 0 40 57 45 847.3 0 45 58 27 847.3 0 27 59 287 847.3 0 287 60 225 847.3 0 225 61 516 847.3 0 516 62 1261 847.3 0 1261 63 265 847.3 0 265 64 549 847.3 0 549 65 1020 847.3 0 1020 66 1335 847.3 0 1335 67 2139 847.3 0 2139 68 906 847.3 0 906 69 719 847.3 0 719 70 211 847.3 0 211 71 341 847.3 0 341 72 1033 847.3 0 1033 73 1114 847.3 0 1114 74 1660 847.3 0 1660 75 2386 847.3 0 2386 76 2600 847.3 0 2600 77 5207 847.3 0 5207 78 7759 847.3 0 7759 79 86476 847.3 0 86476 80 60085 847.3 0 60085 81 169643 847.3 0 169643 82 995 847.3 0 995 83 564 847.3 0 564 84 153 847.3 0 153 85 123 847.3 0 123 86 74 847.3 0 74 87 98 847.3 0 98 88 142 847.3 0 142 89 41 847.3 0 41 90 26 847.3 0 26 91 23 847.3 0 23 92 32 847.3 0 32 93 75 847.3 0 75 94 62 847.3 0 62 95 32 847.3 0 32 96 26 847.3 0 26 97 20 847.3 0 20 98 11 847.3 0 11 99 28 847.3 0 28 100 35 847.3 0 35 101 37 847.3 0 37 102 37 847.3 0 37 103 68 847.3 0 68 104 38 847.3 0 38 105 37 847.3 0 37 106 32 847.3 0 32 107 21 847.3 0 21 108 30 847.3 0 30 109 46 847.3 0 46 110 62 847.3 0 62 111 64 847.3 0 64 112 60 847.3 0 60 113 73 847.3 0 73 114 90 847.3 0 90 115 117 847.3 0 117 116 689 847.3 0 689 117 642 847.3 0 642 118 522 847.3 0 522 119 178 847.3 0 178 120 115 847.3 0 115 121 139 847.3 0 139 122 125 847.3 0 125 123 207 847.3 0 207 124 322 847.3 0 322 125 127 847.3 0 127 126 183 847.3 0 183 127 99 847.3 0 99 128 112 847.3 0 112 129 108 847.3 0 108 130 296 847.3 0 296 131 394 847.3 0 394 132 326 847.3 0 326 133 109 847.3 0 109 134 91 847.3 0 91 135 117 847.3 0 117 136 160 847.3 0 160 137 139 847.3 0 139 138 168 847.3 0 168 139 249 847.3 0 249 140 179 847.3 0 179 141 274 847.3 0 274 142 297 847.3 0 297 143 142 847.3 0 142 144 162 847.3 0 162 145 233 847.3 0 233 146 356 847.3 0 356 147 282 847.3 0 282 148 204 847.3 0 204 149 305 847.3 0 305 150 3164 847.3 0 3164 151 2075 847.3 0 2075 152 1273 847.3 0 1273 153 134 847.3 0 134 154 81 847.3 0 81 155 82 847.3 0 82 156 86 847.3 0 86 157 101 847.3 0 101 158 91 847.3 0 91 159 81 847.3 0 81 160 76 847.3 0 76 161 79 847.3 0 79 162 81 847.3 0 81 163 74 847.3 0 74 164 78 847.3 0 78 165 87 847.3 0 87 166 93 847.3 0 93 167 136 847.3 0 136 168 114 847.3 0 114 169 137 847.3 0 137 170 128 847.3 0 128 171 290 847.3 0 290 172 282 847.3 0 282 173 179 847.3 0 179 174 159 847.3 0 159 175 69 847.3 0 69 176 70 847.3 0 70 177 74 847.3 0 74 178 65 847.3 0 65 179 85 847.3 0 85 180 78 847.3 0 78 181 72 847.3 0 72 182 65 847.3 0 65 183 78 847.3 0 78 184 103 847.3 0 103 185 57 847.3 0 57 186 183 847.3 0 183 187 118 847.3 0 118 188 92 847.3 0 92 189 98 847.3 0 98 190 70 847.3 0 70 191 87 847.3 0 87 192 93 847.3 0 93 193 85 847.3 0 85 194 89 847.3 0 89 195 100 847.3 0 100 196 76 847.3 0 76 197 102 847.3 0 102 198 86 847.3 0 86 199 107 847.3 0 107 200 127 847.3 0 127 201 88 847.3 0 88 202 83 847.3 0 83 203 69 847.3 0 69 204 99 847.3 0 99 205 102 847.3 0 102 206 74 847.3 0 74 207 72 847.3 0 72 208 76 847.3 0 76 209 70 847.3 0 70 210 84 847.3 0 84 211 102 847.3 0 102 212 94 847.3 0 94 213 115 847.3 0 115 214 87 847.3 0 87 215 100 847.3 0 100 216 104 847.3 0 104 217 137 847.3 0 137 218 288 847.3 0 288 219 305 847.3 0 305 220 179 847.3 0 179 221 137 847.3 0 137 222 151 847.3 0 151 223 198 847.3 0 198 224 223 847.3 0 223 225 246 847.3 0 246 226 1011 847.3 0 1011 227 1008 847.3 0 1008 228 1272 847.3 0 1272 229 358 847.3 0 358 230 173 847.3 0 173 231 84 847.3 0 84 232 94 847.3 0 94 233 86 847.3 0 86 234 85 847.3 0 85 235 72 847.3 0 72 236 83 847.3 0 83 237 101 847.3 0 101 238 89 847.3 0 89 239 114 847.3 0 114 240 112 847.3 0 112 241 167 847.3 0 167 242 86 847.3 0 86 243 96 847.3 0 96 244 95 847.3 0 95 245 97 847.3 0 97 246 153 847.3 0 153 247 178 847.3 0 178 248 137 847.3 0 137 249 101 847.3 0 101 250 116 847.3 0 116 251 146 847.3 0 146 252 144 847.3 0 144 253 104 847.3 0 104 254 107 847.3 0 107 255 90 847.3 0 90 256 111 847.3 0 111 257 105 847.3 0 105 258 81 847.3 0 81 259 100 847.3 0 100 260 114 847.3 0 114 261 154 847.3 0 154 262 108 847.3 0 108 263 106 847.3 0 106 264 135 847.3 0 135 265 142 847.3 0 142 266 134 847.3 0 134 267 86 847.3 0 86 268 126 847.3 0 126 269 86 847.3 0 86 270 96 847.3 0 96 271 83 847.3 0 83 272 120 847.3 0 120 273 99 847.3 0 99 274 195 847.3 0 195 275 231 847.3 0 231 276 246 847.3 0 246 277 138 847.3 0 138 278 129 847.3 0 129 279 257 847.3 0 257 280 254 847.3 0 254 281 205 847.3 0 205 282 109 847.3 0 109 283 75 847.3 0 75 284 79 847.3 0 79 285 179 847.3 0 179 286 228 847.3 0 228 287 177 847.3 0 177 288 146 847.3 0 146 289 136 847.3 0 136 290 200 847.3 0 200 291 257 847.3 0 257 292 269 847.3 0 269 293 189 847.3 0 189 294 146 847.3 0 146 295 86 847.3 0 86 296 123 847.3 0 123 297 138 847.3 0 138 298 153 847.3 0 153 299 174 847.3 0 174 300 242 847.3 0 242 301 160 847.3 0 160 Finished in 37.53 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 535,554 Reads with adapters: 80,013 (14.9%) Reads that were too short: 51 (0.0%) Reads written (passing filters): 79,962 (14.9%) Total basepairs processed: 128,937,937 bp Total written (filtered): 21,773,981 bp (16.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 80013 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 0 0 0 3 2 18 59 0.0 3 0 0 46 8 5 19 1022 0.0 3 0 901 57 44 20 20 67352 0.0 4 63988 2713 475 134 42 21 283 0.0 4 38 223 12 8 2 22 10511 0.0 4 9909 471 97 23 11 23 235 0.0 4 11 14 0 141 69 24 3 0.0 4 0 1 0 1 1 25 41 0.0 4 0 0 1 27 13 28 1 0.0 4 1 30 2 0.0 4 1 0 0 0 1 33 1 0.0 4 1 34 1 0.0 4 0 0 0 0 1 39 1 0.0 4 1 43 2 0.0 4 0 0 0 0 2 45 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 49 2 0.0 4 0 0 0 0 2 51 3 0.0 4 0 0 0 0 3 54 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 75 1 0.0 4 1 90 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 95 2 0.0 4 0 0 0 0 2 101 1 0.0 4 0 0 0 0 1 117 2 0.0 4 0 0 0 0 2 118 2 0.0 4 0 0 0 0 2 121 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 125 5 0.0 4 1 0 0 1 3 126 8 0.0 4 0 0 0 0 8 127 407 0.0 4 0 0 0 0 407 128 9 0.0 4 0 0 0 1 8 129 1 0.0 4 1 136 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 1 152 3 0.0 4 0 0 0 0 3 153 5 0.0 4 0 0 0 0 5 156 2 0.0 4 0 0 0 0 2 166 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 207 3 0.0 4 0 0 0 0 3 208 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 234 2 0.0 4 0 0 0 0 2 256 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 2 0.0 4 0 0 0 0 2 275 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.45 s (56 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 79,962 Reads with adapters: 14,021 (17.5%) Reads written (passing filters): 79,962 (100.0%) Total basepairs processed: 21,773,981 bp Total written (filtered): 21,608,910 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 14021 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.4% C: 5.6% G: 90.2% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 93 1249.4 0 93 4 19 312.4 0 19 5 3 78.1 0 3 6 1 19.5 0 1 8 2 1.2 0 2 9 1898 0.3 0 126 1772 10 3567 0.1 1 236 3331 11 8228 0.0 1 184 8044 12 92 0.0 1 2 90 13 9 0.0 1 0 9 14 7 0.0 1 0 7 35 1 0.0 1 0 1 81 1 0.0 1 0 1 93 1 0.0 1 0 1 150 2 0.0 1 1 1 151 9 0.0 1 7 2 157 1 0.0 1 1 159 1 0.0 1 0 1 170 1 0.0 1 1 189 1 0.0 1 0 1 190 1 0.0 1 0 1 208 14 0.0 1 11 3 209 64 0.0 1 50 14 210 1 0.0 1 0 1 211 2 0.0 1 1 1 223 1 0.0 1 1 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.