This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 169.55 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 221,556 (6.4%) Reads that were too short: 14,203 (0.4%) Reads written (passing filters): 207,353 (6.0%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 58,852,819 bp (5.6%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 221556 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3476 847.3 0 3476 7 100258 847.3 0 100258 8 85808 847.3 0 85808 9 132 847.3 0 132 10 55 847.3 0 55 11 37 847.3 0 37 12 39 847.3 0 39 13 69 847.3 0 69 14 40 847.3 0 40 15 56 847.3 0 56 16 72 847.3 0 72 17 61 847.3 0 61 18 55 847.3 0 55 19 40 847.3 0 40 20 36 847.3 0 36 21 25 847.3 0 25 22 43 847.3 0 43 23 39 847.3 0 39 24 55 847.3 0 55 25 50 847.3 0 50 26 52 847.3 0 52 27 60 847.3 0 60 28 195 847.3 0 195 29 30 847.3 0 30 30 35 847.3 0 35 31 52 847.3 0 52 32 49 847.3 0 49 33 39 847.3 0 39 34 34 847.3 0 34 35 38 847.3 0 38 36 46 847.3 0 46 37 52 847.3 0 52 38 49 847.3 0 49 39 44 847.3 0 44 40 33 847.3 0 33 41 28 847.3 0 28 42 50 847.3 0 50 43 41 847.3 0 41 44 39 847.3 0 39 45 34 847.3 0 34 46 41 847.3 0 41 47 48 847.3 0 48 48 39 847.3 0 39 49 28 847.3 0 28 50 37 847.3 0 37 51 50 847.3 0 50 52 35 847.3 0 35 53 28 847.3 0 28 54 34 847.3 0 34 55 35 847.3 0 35 56 34 847.3 0 34 57 27 847.3 0 27 58 50 847.3 0 50 59 47 847.3 0 47 60 39 847.3 0 39 61 36 847.3 0 36 62 31 847.3 0 31 63 45 847.3 0 45 64 36 847.3 0 36 65 48 847.3 0 48 66 44 847.3 0 44 67 45 847.3 0 45 68 33 847.3 0 33 69 35 847.3 0 35 70 31 847.3 0 31 71 40 847.3 0 40 72 37 847.3 0 37 73 55 847.3 0 55 74 66 847.3 0 66 75 127 847.3 0 127 76 83 847.3 0 83 77 61 847.3 0 61 78 59 847.3 0 59 79 76 847.3 0 76 80 69 847.3 0 69 81 60 847.3 0 60 82 54 847.3 0 54 83 60 847.3 0 60 84 43 847.3 0 43 85 48 847.3 0 48 86 80 847.3 0 80 87 44 847.3 0 44 88 28 847.3 0 28 89 54 847.3 0 54 90 51 847.3 0 51 91 92 847.3 0 92 92 81 847.3 0 81 93 66 847.3 0 66 94 111 847.3 0 111 95 70 847.3 0 70 96 71 847.3 0 71 97 101 847.3 0 101 98 265 847.3 0 265 99 236 847.3 0 236 100 260 847.3 0 260 101 105 847.3 0 105 102 80 847.3 0 80 103 62 847.3 0 62 104 82 847.3 0 82 105 105 847.3 0 105 106 119 847.3 0 119 107 142 847.3 0 142 108 396 847.3 0 396 109 313 847.3 0 313 110 310 847.3 0 310 111 143 847.3 0 143 112 169 847.3 0 169 113 139 847.3 0 139 114 200 847.3 0 200 115 177 847.3 0 177 116 242 847.3 0 242 117 127 847.3 0 127 118 76 847.3 0 76 119 74 847.3 0 74 120 97 847.3 0 97 121 81 847.3 0 81 122 138 847.3 0 138 123 131 847.3 0 131 124 118 847.3 0 118 125 80 847.3 0 80 126 108 847.3 0 108 127 81 847.3 0 81 128 105 847.3 0 105 129 139 847.3 0 139 130 147 847.3 0 147 131 205 847.3 0 205 132 122 847.3 0 122 133 112 847.3 0 112 134 113 847.3 0 113 135 146 847.3 0 146 136 122 847.3 0 122 137 151 847.3 0 151 138 137 847.3 0 137 139 172 847.3 0 172 140 156 847.3 0 156 141 146 847.3 0 146 142 123 847.3 0 123 143 134 847.3 0 134 144 153 847.3 0 153 145 143 847.3 0 143 146 118 847.3 0 118 147 102 847.3 0 102 148 89 847.3 0 89 149 113 847.3 0 113 150 115 847.3 0 115 151 101 847.3 0 101 152 83 847.3 0 83 153 98 847.3 0 98 154 75 847.3 0 75 155 164 847.3 0 164 156 140 847.3 0 140 157 166 847.3 0 166 158 102 847.3 0 102 159 110 847.3 0 110 160 127 847.3 0 127 161 112 847.3 0 112 162 105 847.3 0 105 163 135 847.3 0 135 164 138 847.3 0 138 165 140 847.3 0 140 166 114 847.3 0 114 167 121 847.3 0 121 168 90 847.3 0 90 169 92 847.3 0 92 170 80 847.3 0 80 171 97 847.3 0 97 172 98 847.3 0 98 173 83 847.3 0 83 174 75 847.3 0 75 175 82 847.3 0 82 176 99 847.3 0 99 177 77 847.3 0 77 178 135 847.3 0 135 179 126 847.3 0 126 180 101 847.3 0 101 181 106 847.3 0 106 182 106 847.3 0 106 183 113 847.3 0 113 184 119 847.3 0 119 185 101 847.3 0 101 186 90 847.3 0 90 187 93 847.3 0 93 188 89 847.3 0 89 189 101 847.3 0 101 190 104 847.3 0 104 191 100 847.3 0 100 192 105 847.3 0 105 193 88 847.3 0 88 194 98 847.3 0 98 195 103 847.3 0 103 196 107 847.3 0 107 197 85 847.3 0 85 198 105 847.3 0 105 199 103 847.3 0 103 200 130 847.3 0 130 201 133 847.3 0 133 202 152 847.3 0 152 203 156 847.3 0 156 204 125 847.3 0 125 205 120 847.3 0 120 206 99 847.3 0 99 207 145 847.3 0 145 208 156 847.3 0 156 209 169 847.3 0 169 210 266 847.3 0 266 211 143 847.3 0 143 212 104 847.3 0 104 213 90 847.3 0 90 214 93 847.3 0 93 215 105 847.3 0 105 216 123 847.3 0 123 217 105 847.3 0 105 218 101 847.3 0 101 219 119 847.3 0 119 220 119 847.3 0 119 221 131 847.3 0 131 222 160 847.3 0 160 223 166 847.3 0 166 224 172 847.3 0 172 225 115 847.3 0 115 226 109 847.3 0 109 227 111 847.3 0 111 228 86 847.3 0 86 229 92 847.3 0 92 230 83 847.3 0 83 231 87 847.3 0 87 232 110 847.3 0 110 233 104 847.3 0 104 234 77 847.3 0 77 235 102 847.3 0 102 236 119 847.3 0 119 237 116 847.3 0 116 238 107 847.3 0 107 239 140 847.3 0 140 240 108 847.3 0 108 241 137 847.3 0 137 242 186 847.3 0 186 243 203 847.3 0 203 244 196 847.3 0 196 245 154 847.3 0 154 246 210 847.3 0 210 247 123 847.3 0 123 248 78 847.3 0 78 249 100 847.3 0 100 250 77 847.3 0 77 251 98 847.3 0 98 252 120 847.3 0 120 253 239 847.3 0 239 254 267 847.3 0 267 255 648 847.3 0 648 256 143 847.3 0 143 257 98 847.3 0 98 258 133 847.3 0 133 259 148 847.3 0 148 260 136 847.3 0 136 261 111 847.3 0 111 262 125 847.3 0 125 263 231 847.3 0 231 264 276 847.3 0 276 265 261 847.3 0 261 266 89 847.3 0 89 267 61 847.3 0 61 268 77 847.3 0 77 269 141 847.3 0 141 270 172 847.3 0 172 271 279 847.3 0 279 272 238 847.3 0 238 273 186 847.3 0 186 274 91 847.3 0 91 275 103 847.3 0 103 276 105 847.3 0 105 277 81 847.3 0 81 278 88 847.3 0 88 279 95 847.3 0 95 280 90 847.3 0 90 281 97 847.3 0 97 282 119 847.3 0 119 283 143 847.3 0 143 284 151 847.3 0 151 285 196 847.3 0 196 286 194 847.3 0 194 287 112 847.3 0 112 288 91 847.3 0 91 289 96 847.3 0 96 290 254 847.3 0 254 291 279 847.3 0 279 292 188 847.3 0 188 293 126 847.3 0 126 294 111 847.3 0 111 295 91 847.3 0 91 296 114 847.3 0 114 297 123 847.3 0 123 298 128 847.3 0 128 299 130 847.3 0 130 300 128 847.3 0 128 301 153 847.3 0 153 Finished in 16.59 s (80 us/read; 0.75 M reads/minute). === Summary === Total reads processed: 207,353 Reads with adapters: 88,167 (42.5%) Reads that were too short: 61 (0.0%) Reads written (passing filters): 88,106 (42.5%) Total basepairs processed: 58,852,819 bp Total written (filtered): 24,030,995 bp (40.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 88167 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 7 0.0 3 0 0 0 3 4 18 42 0.0 3 0 0 24 11 7 19 905 0.0 3 0 719 102 62 22 20 86402 0.0 4 81997 3560 621 171 53 21 240 0.0 4 41 184 10 4 1 22 28 0.0 4 7 1 11 2 7 23 239 0.0 4 4 0 1 162 72 24 10 0.0 4 0 0 0 2 8 25 3 0.0 4 1 1 0 0 1 26 13 0.0 4 1 1 0 1 10 30 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 54 1 0.0 4 1 72 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 121 3 0.0 4 1 1 0 0 1 126 2 0.0 4 0 0 0 0 2 127 193 0.0 4 0 0 0 0 193 128 3 0.0 4 0 0 0 0 3 129 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 153 9 0.0 4 0 0 0 0 9 176 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 1 212 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 228 8 0.0 4 0 0 0 1 7 229 3 0.0 4 0 0 0 0 3 230 1 0.0 4 0 0 0 0 1 234 3 0.0 4 0 0 0 0 3 235 3 0.0 4 0 0 0 0 3 236 1 0.0 4 0 0 0 0 1 237 2 0.0 4 0 0 0 0 2 252 1 0.0 4 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 3 0.0 4 0 0 0 0 3 258 1 0.0 4 0 0 0 0 1 260 2 0.0 4 0 0 0 0 2 261 2 0.0 4 0 0 0 0 2 262 3 0.0 4 0 0 0 0 3 263 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 282 3 0.0 4 0 0 0 0 3 286 1 0.0 4 0 0 0 0 1 287 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 292 3 0.0 4 0 0 0 0 3 294 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.34 s (61 us/read; 0.99 M reads/minute). === Summary === Total reads processed: 88,106 Reads with adapters: 15,053 (17.1%) Reads written (passing filters): 88,106 (100.0%) Total basepairs processed: 24,030,995 bp Total written (filtered): 23,813,939 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 15053 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.7% C: 6.0% G: 88.4% T: 2.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 138 1376.7 0 138 4 25 344.2 0 25 5 2 86.0 0 2 7 2 5.4 0 2 8 1 1.3 0 1 9 178 0.3 0 14 164 10 4049 0.1 1 274 3775 11 10266 0.0 1 231 10035 12 75 0.0 1 1 74 13 10 0.0 1 0 10 14 18 0.0 1 1 17 15 4 0.0 1 0 4 16 1 0.0 1 0 1 123 1 0.0 1 1 151 26 0.0 1 25 1 152 2 0.0 1 2 159 1 0.0 1 0 1 178 1 0.0 1 1 190 1 0.0 1 0 1 208 1 0.0 1 0 1 209 80 0.0 1 66 14 210 1 0.0 1 1 211 1 0.0 1 1 212 1 0.0 1 1 224 158 0.0 1 143 15 225 6 0.0 1 6 226 3 0.0 1 3 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.