This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 167.29 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 222,278 (6.4%) Reads that were too short: 12,351 (0.4%) Reads written (passing filters): 209,927 (6.0%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 59,846,558 bp (5.7%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 222278 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2036 847.3 0 2036 7 93561 847.3 0 93561 8 99624 847.3 0 99624 9 81 847.3 0 81 10 39 847.3 0 39 11 28 847.3 0 28 12 24 847.3 0 24 13 31 847.3 0 31 14 22 847.3 0 22 15 22 847.3 0 22 16 29 847.3 0 29 17 37 847.3 0 37 18 39 847.3 0 39 19 25 847.3 0 25 20 19 847.3 0 19 21 16 847.3 0 16 22 20 847.3 0 20 23 22 847.3 0 22 24 20 847.3 0 20 25 18 847.3 0 18 26 17 847.3 0 17 27 16 847.3 0 16 28 46 847.3 0 46 29 17 847.3 0 17 30 21 847.3 0 21 31 20 847.3 0 20 32 19 847.3 0 19 33 19 847.3 0 19 34 23 847.3 0 23 35 25 847.3 0 25 36 24 847.3 0 24 37 27 847.3 0 27 38 35 847.3 0 35 39 29 847.3 0 29 40 16 847.3 0 16 41 33 847.3 0 33 42 21 847.3 0 21 43 24 847.3 0 24 44 22 847.3 0 22 45 15 847.3 0 15 46 25 847.3 0 25 47 25 847.3 0 25 48 25 847.3 0 25 49 24 847.3 0 24 50 25 847.3 0 25 51 27 847.3 0 27 52 19 847.3 0 19 53 17 847.3 0 17 54 19 847.3 0 19 55 16 847.3 0 16 56 21 847.3 0 21 57 13 847.3 0 13 58 23 847.3 0 23 59 24 847.3 0 24 60 20 847.3 0 20 61 24 847.3 0 24 62 25 847.3 0 25 63 26 847.3 0 26 64 32 847.3 0 32 65 30 847.3 0 30 66 16 847.3 0 16 67 15 847.3 0 15 68 22 847.3 0 22 69 28 847.3 0 28 70 13 847.3 0 13 71 26 847.3 0 26 72 18 847.3 0 18 73 13 847.3 0 13 74 19 847.3 0 19 75 38 847.3 0 38 76 25 847.3 0 25 77 30 847.3 0 30 78 48 847.3 0 48 79 51 847.3 0 51 80 68 847.3 0 68 81 66 847.3 0 66 82 53 847.3 0 53 83 78 847.3 0 78 84 70 847.3 0 70 85 65 847.3 0 65 86 68 847.3 0 68 87 44 847.3 0 44 88 60 847.3 0 60 89 48 847.3 0 48 90 62 847.3 0 62 91 78 847.3 0 78 92 104 847.3 0 104 93 85 847.3 0 85 94 84 847.3 0 84 95 89 847.3 0 89 96 72 847.3 0 72 97 81 847.3 0 81 98 144 847.3 0 144 99 142 847.3 0 142 100 164 847.3 0 164 101 93 847.3 0 93 102 79 847.3 0 79 103 71 847.3 0 71 104 73 847.3 0 73 105 94 847.3 0 94 106 112 847.3 0 112 107 146 847.3 0 146 108 351 847.3 0 351 109 280 847.3 0 280 110 268 847.3 0 268 111 109 847.3 0 109 112 157 847.3 0 157 113 117 847.3 0 117 114 235 847.3 0 235 115 179 847.3 0 179 116 257 847.3 0 257 117 95 847.3 0 95 118 78 847.3 0 78 119 47 847.3 0 47 120 68 847.3 0 68 121 76 847.3 0 76 122 120 847.3 0 120 123 142 847.3 0 142 124 115 847.3 0 115 125 87 847.3 0 87 126 72 847.3 0 72 127 77 847.3 0 77 128 69 847.3 0 69 129 123 847.3 0 123 130 130 847.3 0 130 131 183 847.3 0 183 132 115 847.3 0 115 133 118 847.3 0 118 134 121 847.3 0 121 135 124 847.3 0 124 136 134 847.3 0 134 137 172 847.3 0 172 138 114 847.3 0 114 139 121 847.3 0 121 140 123 847.3 0 123 141 131 847.3 0 131 142 82 847.3 0 82 143 93 847.3 0 93 144 96 847.3 0 96 145 103 847.3 0 103 146 86 847.3 0 86 147 73 847.3 0 73 148 82 847.3 0 82 149 93 847.3 0 93 150 100 847.3 0 100 151 91 847.3 0 91 152 98 847.3 0 98 153 107 847.3 0 107 154 97 847.3 0 97 155 175 847.3 0 175 156 129 847.3 0 129 157 138 847.3 0 138 158 89 847.3 0 89 159 83 847.3 0 83 160 100 847.3 0 100 161 87 847.3 0 87 162 84 847.3 0 84 163 119 847.3 0 119 164 128 847.3 0 128 165 140 847.3 0 140 166 91 847.3 0 91 167 121 847.3 0 121 168 91 847.3 0 91 169 102 847.3 0 102 170 90 847.3 0 90 171 86 847.3 0 86 172 86 847.3 0 86 173 66 847.3 0 66 174 54 847.3 0 54 175 78 847.3 0 78 176 94 847.3 0 94 177 82 847.3 0 82 178 124 847.3 0 124 179 122 847.3 0 122 180 89 847.3 0 89 181 94 847.3 0 94 182 112 847.3 0 112 183 106 847.3 0 106 184 99 847.3 0 99 185 81 847.3 0 81 186 79 847.3 0 79 187 76 847.3 0 76 188 101 847.3 0 101 189 94 847.3 0 94 190 100 847.3 0 100 191 117 847.3 0 117 192 101 847.3 0 101 193 75 847.3 0 75 194 90 847.3 0 90 195 88 847.3 0 88 196 88 847.3 0 88 197 93 847.3 0 93 198 63 847.3 0 63 199 73 847.3 0 73 200 112 847.3 0 112 201 131 847.3 0 131 202 153 847.3 0 153 203 152 847.3 0 152 204 106 847.3 0 106 205 123 847.3 0 123 206 102 847.3 0 102 207 112 847.3 0 112 208 124 847.3 0 124 209 187 847.3 0 187 210 209 847.3 0 209 211 157 847.3 0 157 212 81 847.3 0 81 213 76 847.3 0 76 214 74 847.3 0 74 215 71 847.3 0 71 216 114 847.3 0 114 217 88 847.3 0 88 218 85 847.3 0 85 219 104 847.3 0 104 220 82 847.3 0 82 221 98 847.3 0 98 222 123 847.3 0 123 223 128 847.3 0 128 224 149 847.3 0 149 225 105 847.3 0 105 226 93 847.3 0 93 227 89 847.3 0 89 228 79 847.3 0 79 229 80 847.3 0 80 230 86 847.3 0 86 231 80 847.3 0 80 232 89 847.3 0 89 233 69 847.3 0 69 234 60 847.3 0 60 235 77 847.3 0 77 236 97 847.3 0 97 237 121 847.3 0 121 238 90 847.3 0 90 239 130 847.3 0 130 240 103 847.3 0 103 241 120 847.3 0 120 242 157 847.3 0 157 243 158 847.3 0 158 244 173 847.3 0 173 245 163 847.3 0 163 246 224 847.3 0 224 247 110 847.3 0 110 248 90 847.3 0 90 249 77 847.3 0 77 250 87 847.3 0 87 251 85 847.3 0 85 252 132 847.3 0 132 253 268 847.3 0 268 254 301 847.3 0 301 255 559 847.3 0 559 256 140 847.3 0 140 257 80 847.3 0 80 258 108 847.3 0 108 259 150 847.3 0 150 260 140 847.3 0 140 261 84 847.3 0 84 262 91 847.3 0 91 263 191 847.3 0 191 264 198 847.3 0 198 265 223 847.3 0 223 266 75 847.3 0 75 267 63 847.3 0 63 268 61 847.3 0 61 269 127 847.3 0 127 270 133 847.3 0 133 271 207 847.3 0 207 272 235 847.3 0 235 273 155 847.3 0 155 274 120 847.3 0 120 275 89 847.3 0 89 276 94 847.3 0 94 277 93 847.3 0 93 278 65 847.3 0 65 279 59 847.3 0 59 280 73 847.3 0 73 281 119 847.3 0 119 282 110 847.3 0 110 283 116 847.3 0 116 284 155 847.3 0 155 285 159 847.3 0 159 286 101 847.3 0 101 287 78 847.3 0 78 288 73 847.3 0 73 289 91 847.3 0 91 290 213 847.3 0 213 291 194 847.3 0 194 292 125 847.3 0 125 293 109 847.3 0 109 294 71 847.3 0 71 295 67 847.3 0 67 296 102 847.3 0 102 297 98 847.3 0 98 298 130 847.3 0 130 299 129 847.3 0 129 300 112 847.3 0 112 301 95 847.3 0 95 Finished in 15.53 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 209,927 Reads with adapters: 93,243 (44.4%) Reads that were too short: 35 (0.0%) Reads written (passing filters): 93,208 (44.4%) Total basepairs processed: 59,846,558 bp Total written (filtered): 25,816,209 bp (43.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 93243 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 14 0.0 3 0 0 11 3 16 413 0.0 3 0 404 8 1 17 92422 0.0 3 90790 1579 49 4 18 180 0.0 3 20 149 11 19 36 0.0 3 3 1 26 6 20 118 0.0 3 0 0 0 118 21 1 0.0 3 1 23 1 0.0 3 0 0 0 1 26 2 0.0 3 1 0 0 1 28 2 0.0 3 0 0 0 2 29 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 60 3 0.0 3 0 0 0 3 61 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 122 1 0.0 3 1 123 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 279 5 0.0 3 0 0 0 5 280 3 0.0 3 0 1 0 2 281 2 0.0 3 0 0 0 2 282 5 0.0 3 0 0 0 5 283 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.46 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 93,208 Reads with adapters: 429 (0.5%) Reads written (passing filters): 93,208 (100.0%) Total basepairs processed: 25,816,209 bp Total written (filtered): 25,814,841 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 429 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 48.3% C: 14.5% G: 18.4% T: 18.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 360 1456.4 0 360 4 59 364.1 0 59 5 8 91.0 0 8 6 2 22.8 0 2