This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 169.65 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 360,809 (10.4%) Reads that were too short: 36,487 (1.1%) Reads written (passing filters): 324,322 (9.3%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 93,251,222 bp (8.9%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 360809 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2120 847.3 0 2120 7 140574 847.3 0 140574 8 2230 847.3 0 2230 9 166037 847.3 0 166037 10 153 847.3 0 153 11 27 847.3 0 27 12 32 847.3 0 32 13 19 847.3 0 19 14 9 847.3 0 9 15 11 847.3 0 11 16 17 847.3 0 17 17 9 847.3 0 9 18 13 847.3 0 13 19 9 847.3 0 9 20 17 847.3 0 17 21 17 847.3 0 17 22 9 847.3 0 9 23 7 847.3 0 7 24 12 847.3 0 12 25 8 847.3 0 8 26 15 847.3 0 15 27 13 847.3 0 13 28 12 847.3 0 12 29 10 847.3 0 10 30 19 847.3 0 19 31 15 847.3 0 15 32 7 847.3 0 7 33 17 847.3 0 17 34 15 847.3 0 15 35 14 847.3 0 14 36 24 847.3 0 24 37 40 847.3 0 40 38 17 847.3 0 17 39 20 847.3 0 20 40 16 847.3 0 16 41 18 847.3 0 18 42 20 847.3 0 20 43 15 847.3 0 15 44 23 847.3 0 23 45 16 847.3 0 16 46 11 847.3 0 11 47 20 847.3 0 20 48 24 847.3 0 24 49 21 847.3 0 21 50 7 847.3 0 7 51 10 847.3 0 10 52 11 847.3 0 11 53 18 847.3 0 18 54 14 847.3 0 14 55 33 847.3 0 33 56 30 847.3 0 30 57 23 847.3 0 23 58 15 847.3 0 15 59 20 847.3 0 20 60 22 847.3 0 22 61 22 847.3 0 22 62 27 847.3 0 27 63 14 847.3 0 14 64 18 847.3 0 18 65 21 847.3 0 21 66 22 847.3 0 22 67 24 847.3 0 24 68 20 847.3 0 20 69 25 847.3 0 25 70 30 847.3 0 30 71 27 847.3 0 27 72 27 847.3 0 27 73 26 847.3 0 26 74 122 847.3 0 122 75 130 847.3 0 130 76 215 847.3 0 215 77 19 847.3 0 19 78 22 847.3 0 22 79 21 847.3 0 21 80 54 847.3 0 54 81 88 847.3 0 88 82 276 847.3 0 276 83 286 847.3 0 286 84 381 847.3 0 381 85 44 847.3 0 44 86 53 847.3 0 53 87 34 847.3 0 34 88 46 847.3 0 46 89 39 847.3 0 39 90 25 847.3 0 25 91 34 847.3 0 34 92 29 847.3 0 29 93 20 847.3 0 20 94 13 847.3 0 13 95 31 847.3 0 31 96 27 847.3 0 27 97 35 847.3 0 35 98 25 847.3 0 25 99 33 847.3 0 33 100 30 847.3 0 30 101 37 847.3 0 37 102 26 847.3 0 26 103 42 847.3 0 42 104 38 847.3 0 38 105 31 847.3 0 31 106 41 847.3 0 41 107 36 847.3 0 36 108 40 847.3 0 40 109 26 847.3 0 26 110 29 847.3 0 29 111 47 847.3 0 47 112 35 847.3 0 35 113 41 847.3 0 41 114 34 847.3 0 34 115 31 847.3 0 31 116 27 847.3 0 27 117 32 847.3 0 32 118 32 847.3 0 32 119 30 847.3 0 30 120 40 847.3 0 40 121 67 847.3 0 67 122 97 847.3 0 97 123 96 847.3 0 96 124 37 847.3 0 37 125 77 847.3 0 77 126 108 847.3 0 108 127 52 847.3 0 52 128 55 847.3 0 55 129 37 847.3 0 37 130 26 847.3 0 26 131 38 847.3 0 38 132 46 847.3 0 46 133 53 847.3 0 53 134 40 847.3 0 40 135 51 847.3 0 51 136 42 847.3 0 42 137 54 847.3 0 54 138 99 847.3 0 99 139 49 847.3 0 49 140 217 847.3 0 217 141 234 847.3 0 234 142 49 847.3 0 49 143 54 847.3 0 54 144 40 847.3 0 40 145 49 847.3 0 49 146 51 847.3 0 51 147 50 847.3 0 50 148 45 847.3 0 45 149 59 847.3 0 59 150 52 847.3 0 52 151 48 847.3 0 48 152 37 847.3 0 37 153 71 847.3 0 71 154 46 847.3 0 46 155 61 847.3 0 61 156 64 847.3 0 64 157 71 847.3 0 71 158 50 847.3 0 50 159 60 847.3 0 60 160 63 847.3 0 63 161 67 847.3 0 67 162 53 847.3 0 53 163 69 847.3 0 69 164 99 847.3 0 99 165 92 847.3 0 92 166 100 847.3 0 100 167 169 847.3 0 169 168 442 847.3 0 442 169 1442 847.3 0 1442 170 89 847.3 0 89 171 266 847.3 0 266 172 189 847.3 0 189 173 293 847.3 0 293 174 91 847.3 0 91 175 68 847.3 0 68 176 76 847.3 0 76 177 78 847.3 0 78 178 57 847.3 0 57 179 84 847.3 0 84 180 103 847.3 0 103 181 94 847.3 0 94 182 132 847.3 0 132 183 109 847.3 0 109 184 106 847.3 0 106 185 83 847.3 0 83 186 118 847.3 0 118 187 93 847.3 0 93 188 100 847.3 0 100 189 119 847.3 0 119 190 102 847.3 0 102 191 125 847.3 0 125 192 99 847.3 0 99 193 94 847.3 0 94 194 104 847.3 0 104 195 101 847.3 0 101 196 95 847.3 0 95 197 102 847.3 0 102 198 130 847.3 0 130 199 122 847.3 0 122 200 261 847.3 0 261 201 426 847.3 0 426 202 424 847.3 0 424 203 194 847.3 0 194 204 109 847.3 0 109 205 121 847.3 0 121 206 105 847.3 0 105 207 108 847.3 0 108 208 93 847.3 0 93 209 102 847.3 0 102 210 128 847.3 0 128 211 135 847.3 0 135 212 114 847.3 0 114 213 113 847.3 0 113 214 126 847.3 0 126 215 142 847.3 0 142 216 216 847.3 0 216 217 167 847.3 0 167 218 155 847.3 0 155 219 128 847.3 0 128 220 130 847.3 0 130 221 152 847.3 0 152 222 151 847.3 0 151 223 151 847.3 0 151 224 192 847.3 0 192 225 237 847.3 0 237 226 326 847.3 0 326 227 458 847.3 0 458 228 3928 847.3 0 3928 229 3632 847.3 0 3632 230 5827 847.3 0 5827 231 291 847.3 0 291 232 131 847.3 0 131 233 107 847.3 0 107 234 117 847.3 0 117 235 117 847.3 0 117 236 110 847.3 0 110 237 110 847.3 0 110 238 121 847.3 0 121 239 135 847.3 0 135 240 123 847.3 0 123 241 105 847.3 0 105 242 123 847.3 0 123 243 159 847.3 0 159 244 142 847.3 0 142 245 133 847.3 0 133 246 184 847.3 0 184 247 210 847.3 0 210 248 219 847.3 0 219 249 143 847.3 0 143 250 162 847.3 0 162 251 118 847.3 0 118 252 131 847.3 0 131 253 142 847.3 0 142 254 237 847.3 0 237 255 153 847.3 0 153 256 387 847.3 0 387 257 462 847.3 0 462 258 400 847.3 0 400 259 203 847.3 0 203 260 211 847.3 0 211 261 901 847.3 0 901 262 2042 847.3 0 2042 263 1439 847.3 0 1439 264 832 847.3 0 832 265 523 847.3 0 523 266 196 847.3 0 196 267 233 847.3 0 233 268 659 847.3 0 659 269 781 847.3 0 781 270 476 847.3 0 476 271 199 847.3 0 199 272 177 847.3 0 177 273 160 847.3 0 160 274 114 847.3 0 114 275 153 847.3 0 153 276 181 847.3 0 181 277 153 847.3 0 153 278 124 847.3 0 124 279 121 847.3 0 121 280 132 847.3 0 132 281 187 847.3 0 187 282 229 847.3 0 229 283 211 847.3 0 211 284 154 847.3 0 154 285 136 847.3 0 136 286 177 847.3 0 177 287 237 847.3 0 237 288 278 847.3 0 278 289 168 847.3 0 168 290 110 847.3 0 110 291 131 847.3 0 131 292 158 847.3 0 158 293 192 847.3 0 192 294 202 847.3 0 202 295 129 847.3 0 129 296 127 847.3 0 127 297 116 847.3 0 116 298 141 847.3 0 141 299 144 847.3 0 144 300 175 847.3 0 175 301 239 847.3 0 239 Finished in 24.22 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 324,322 Reads with adapters: 140,332 (43.3%) Reads that were too short: 50 (0.0%) Reads written (passing filters): 140,282 (43.3%) Total basepairs processed: 93,251,222 bp Total written (filtered): 38,852,843 bp (41.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 140332 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 19 0.0 3 0 0 19 16 1030 0.0 3 0 1002 23 5 17 138688 0.0 3 136265 2340 76 7 18 263 0.0 3 32 223 7 1 19 50 0.0 3 0 1 46 3 20 176 0.0 3 1 0 0 175 21 1 0.0 3 0 0 1 28 2 0.0 3 2 31 21 0.0 3 0 0 0 21 34 1 0.0 3 1 36 1 0.0 3 1 38 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 71 3 0.0 3 0 0 0 3 87 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 4 0.0 3 0 0 0 4 164 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 192 6 0.0 3 0 0 0 6 194 6 0.0 3 0 0 0 6 195 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 2 0.0 3 0 0 0 2 261 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 1 278 2 0.0 3 0 0 0 2 279 1 0.0 3 0 0 0 1 280 8 0.0 3 0 0 0 8 281 4 0.0 3 0 0 0 4 282 3 0.0 3 0 0 0 3 283 2 0.0 3 0 0 0 2 286 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 8.07 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 140,282 Reads with adapters: 537 (0.4%) Reads written (passing filters): 140,282 (100.0%) Total basepairs processed: 38,852,843 bp Total written (filtered): 38,851,043 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 537 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 69.1% C: 7.6% G: 12.5% T: 10.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 375 2191.9 0 375 4 147 548.0 0 147 5 9 137.0 0 9 6 5 34.2 0 5 12 1 0.0 1 0 1