This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 170.45 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 270,850 (7.8%) Reads that were too short: 26,883 (0.8%) Reads written (passing filters): 243,967 (7.0%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 62,613,758 bp (6.0%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 270850 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1010 847.3 0 1010 7 1909 847.3 0 1909 8 69065 847.3 0 69065 9 62139 847.3 0 62139 10 131 847.3 0 131 11 63 847.3 0 63 12 66 847.3 0 66 13 33 847.3 0 33 14 35 847.3 0 35 15 38 847.3 0 38 16 38 847.3 0 38 17 61 847.3 0 61 18 69 847.3 0 69 19 38 847.3 0 38 20 38 847.3 0 38 21 41 847.3 0 41 22 43 847.3 0 43 23 39 847.3 0 39 24 43 847.3 0 43 25 49 847.3 0 49 26 48 847.3 0 48 27 30 847.3 0 30 28 32 847.3 0 32 29 39 847.3 0 39 30 60 847.3 0 60 31 52 847.3 0 52 32 61 847.3 0 61 33 61 847.3 0 61 34 49 847.3 0 49 35 95 847.3 0 95 36 73 847.3 0 73 37 54 847.3 0 54 38 60 847.3 0 60 39 91 847.3 0 91 40 45 847.3 0 45 41 85 847.3 0 85 42 264 847.3 0 264 43 70 847.3 0 70 44 53 847.3 0 53 45 63 847.3 0 63 46 47 847.3 0 47 47 35 847.3 0 35 48 53 847.3 0 53 49 146 847.3 0 146 50 109 847.3 0 109 51 215 847.3 0 215 52 405 847.3 0 405 53 72 847.3 0 72 54 1122 847.3 0 1122 55 260 847.3 0 260 56 167 847.3 0 167 57 365 847.3 0 365 58 330 847.3 0 330 59 278 847.3 0 278 60 84 847.3 0 84 61 108 847.3 0 108 62 206 847.3 0 206 63 195 847.3 0 195 64 344 847.3 0 344 65 581 847.3 0 581 66 587 847.3 0 587 67 1439 847.3 0 1439 68 3025 847.3 0 3025 69 38876 847.3 0 38876 70 11551 847.3 0 11551 71 17250 847.3 0 17250 72 105 847.3 0 105 73 52 847.3 0 52 74 62 847.3 0 62 75 174 847.3 0 174 76 143 847.3 0 143 77 77 847.3 0 77 78 76 847.3 0 76 79 77 847.3 0 77 80 212 847.3 0 212 81 205 847.3 0 205 82 368 847.3 0 368 83 63 847.3 0 63 84 59 847.3 0 59 85 77 847.3 0 77 86 100 847.3 0 100 87 125 847.3 0 125 88 138 847.3 0 138 89 101 847.3 0 101 90 114 847.3 0 114 91 89 847.3 0 89 92 60 847.3 0 60 93 93 847.3 0 93 94 446 847.3 0 446 95 176 847.3 0 176 96 104 847.3 0 104 97 127 847.3 0 127 98 67 847.3 0 67 99 153 847.3 0 153 100 999 847.3 0 999 101 1207 847.3 0 1207 102 1471 847.3 0 1471 103 119 847.3 0 119 104 90 847.3 0 90 105 56 847.3 0 56 106 43 847.3 0 43 107 52 847.3 0 52 108 60 847.3 0 60 109 50 847.3 0 50 110 96 847.3 0 96 111 100 847.3 0 100 112 81 847.3 0 81 113 126 847.3 0 126 114 123 847.3 0 123 115 95 847.3 0 95 116 96 847.3 0 96 117 132 847.3 0 132 118 157 847.3 0 157 119 138 847.3 0 138 120 102 847.3 0 102 121 98 847.3 0 98 122 138 847.3 0 138 123 154 847.3 0 154 124 126 847.3 0 126 125 108 847.3 0 108 126 130 847.3 0 130 127 107 847.3 0 107 128 110 847.3 0 110 129 107 847.3 0 107 130 116 847.3 0 116 131 225 847.3 0 225 132 1017 847.3 0 1017 133 580 847.3 0 580 134 330 847.3 0 330 135 145 847.3 0 145 136 576 847.3 0 576 137 538 847.3 0 538 138 403 847.3 0 403 139 209 847.3 0 209 140 151 847.3 0 151 141 106 847.3 0 106 142 109 847.3 0 109 143 126 847.3 0 126 144 131 847.3 0 131 145 138 847.3 0 138 146 146 847.3 0 146 147 93 847.3 0 93 148 116 847.3 0 116 149 115 847.3 0 115 150 131 847.3 0 131 151 139 847.3 0 139 152 156 847.3 0 156 153 134 847.3 0 134 154 161 847.3 0 161 155 137 847.3 0 137 156 123 847.3 0 123 157 218 847.3 0 218 158 274 847.3 0 274 159 312 847.3 0 312 160 240 847.3 0 240 161 239 847.3 0 239 162 365 847.3 0 365 163 145 847.3 0 145 164 161 847.3 0 161 165 154 847.3 0 154 166 313 847.3 0 313 167 244 847.3 0 244 168 410 847.3 0 410 169 213 847.3 0 213 170 208 847.3 0 208 171 168 847.3 0 168 172 193 847.3 0 193 173 167 847.3 0 167 174 150 847.3 0 150 175 119 847.3 0 119 176 256 847.3 0 256 177 837 847.3 0 837 178 790 847.3 0 790 179 747 847.3 0 747 180 261 847.3 0 261 181 224 847.3 0 224 182 571 847.3 0 571 183 849 847.3 0 849 184 1172 847.3 0 1172 185 412 847.3 0 412 186 220 847.3 0 220 187 170 847.3 0 170 188 189 847.3 0 189 189 260 847.3 0 260 190 207 847.3 0 207 191 158 847.3 0 158 192 156 847.3 0 156 193 124 847.3 0 124 194 151 847.3 0 151 195 139 847.3 0 139 196 153 847.3 0 153 197 200 847.3 0 200 198 222 847.3 0 222 199 214 847.3 0 214 200 179 847.3 0 179 201 165 847.3 0 165 202 924 847.3 0 924 203 925 847.3 0 925 204 805 847.3 0 805 205 395 847.3 0 395 206 133 847.3 0 133 207 111 847.3 0 111 208 105 847.3 0 105 209 141 847.3 0 141 210 149 847.3 0 149 211 197 847.3 0 197 212 195 847.3 0 195 213 230 847.3 0 230 214 164 847.3 0 164 215 152 847.3 0 152 216 139 847.3 0 139 217 203 847.3 0 203 218 221 847.3 0 221 219 168 847.3 0 168 220 141 847.3 0 141 221 163 847.3 0 163 222 439 847.3 0 439 223 798 847.3 0 798 224 797 847.3 0 797 225 584 847.3 0 584 226 219 847.3 0 219 227 149 847.3 0 149 228 202 847.3 0 202 229 208 847.3 0 208 230 129 847.3 0 129 231 117 847.3 0 117 232 137 847.3 0 137 233 129 847.3 0 129 234 123 847.3 0 123 235 117 847.3 0 117 236 144 847.3 0 144 237 355 847.3 0 355 238 153 847.3 0 153 239 159 847.3 0 159 240 134 847.3 0 134 241 157 847.3 0 157 242 502 847.3 0 502 243 537 847.3 0 537 244 429 847.3 0 429 245 269 847.3 0 269 246 156 847.3 0 156 247 140 847.3 0 140 248 102 847.3 0 102 249 96 847.3 0 96 250 152 847.3 0 152 251 388 847.3 0 388 252 300 847.3 0 300 253 203 847.3 0 203 254 145 847.3 0 145 255 218 847.3 0 218 256 267 847.3 0 267 257 295 847.3 0 295 258 265 847.3 0 265 259 194 847.3 0 194 260 197 847.3 0 197 261 163 847.3 0 163 262 156 847.3 0 156 263 224 847.3 0 224 264 214 847.3 0 214 265 355 847.3 0 355 266 323 847.3 0 323 267 233 847.3 0 233 268 141 847.3 0 141 269 712 847.3 0 712 270 710 847.3 0 710 271 509 847.3 0 509 272 422 847.3 0 422 273 443 847.3 0 443 274 244 847.3 0 244 275 234 847.3 0 234 276 270 847.3 0 270 277 358 847.3 0 358 278 323 847.3 0 323 279 210 847.3 0 210 280 142 847.3 0 142 281 121 847.3 0 121 282 151 847.3 0 151 283 184 847.3 0 184 284 197 847.3 0 197 285 231 847.3 0 231 286 254 847.3 0 254 287 158 847.3 0 158 288 163 847.3 0 163 289 149 847.3 0 149 290 172 847.3 0 172 291 345 847.3 0 345 292 478 847.3 0 478 293 355 847.3 0 355 294 240 847.3 0 240 295 131 847.3 0 131 296 143 847.3 0 143 297 173 847.3 0 173 298 183 847.3 0 183 299 260 847.3 0 260 300 326 847.3 0 326 301 317 847.3 0 317 Finished in 18.21 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 243,967 Reads with adapters: 64,879 (26.6%) Reads that were too short: 72 (0.0%) Reads written (passing filters): 64,807 (26.6%) Total basepairs processed: 62,613,758 bp Total written (filtered): 17,614,867 bp (28.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 64879 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 9 0.0 3 0 0 0 7 2 18 49 0.0 3 0 0 38 7 4 19 1218 0.0 3 0 1088 70 41 19 20 62940 0.0 4 59627 2647 489 145 32 21 146 0.0 4 27 102 11 4 2 22 29 0.0 4 7 0 15 0 7 23 248 0.0 4 6 3 0 158 81 24 7 0.0 4 1 0 0 1 5 25 2 0.0 4 1 0 0 0 1 27 1 0.0 4 0 0 0 1 28 2 0.0 4 0 0 0 0 2 34 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 36 1 0.0 4 1 39 1 0.0 4 0 0 0 0 1 40 2 0.0 4 0 0 0 0 2 45 1 0.0 4 0 0 0 0 1 46 2 0.0 4 0 0 0 0 2 47 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 63 2 0.0 4 0 0 0 0 2 64 1 0.0 4 0 0 0 0 1 65 2 0.0 4 0 0 0 0 2 68 1 0.0 4 0 0 0 0 1 77 2 0.0 4 0 0 0 0 2 79 1 0.0 4 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 84 2 0.0 4 0 0 0 0 2 86 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 96 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 100 1 0.0 4 1 106 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 115 2 0.0 4 0 0 0 0 2 118 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 125 1 0.0 4 1 126 2 0.0 4 0 0 0 0 2 127 124 0.0 4 0 0 0 0 124 128 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 139 4 0.0 4 0 0 0 0 4 142 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 153 4 0.0 4 0 0 0 0 4 154 1 0.0 4 0 1 161 2 0.0 4 0 0 0 0 2 166 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 215 3 0.0 4 0 0 0 0 3 217 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 3 0.0 4 0 0 0 0 3 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 2 0.0 4 0 0 0 0 2 257 2 0.0 4 0 0 0 0 2 260 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 0 2 265 4 0.0 4 0 0 0 0 4 268 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.09 s (63 us/read; 0.95 M reads/minute). === Summary === Total reads processed: 64,807 Reads with adapters: 18,748 (28.9%) Reads written (passing filters): 64,807 (100.0%) Total basepairs processed: 17,614,867 bp Total written (filtered): 17,385,900 bp (98.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 18748 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.5% C: 5.1% G: 91.1% T: 2.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 30 1012.6 0 30 4 15 253.2 0 15 6 19 15.8 0 19 7 2 4.0 0 2 8 4 1.0 0 4 9 10735 0.2 0 798 9937 10 5254 0.1 1 308 4946 11 2363 0.0 1 54 2309 12 64 0.0 1 1 63 13 18 0.0 1 0 18 14 2 0.0 1 0 2 72 1 0.0 1 0 1 73 1 0.0 1 1 150 9 0.0 1 6 3 153 1 0.0 1 1 169 1 0.0 1 1 208 25 0.0 1 16 9 209 2 0.0 1 2 210 1 0.0 1 0 1 220 2 0.0 1 1 1 223 188 0.0 1 175 13 224 7 0.0 1 7 225 2 0.0 1 2 226 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.