This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 172.31 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 280,062 (8.1%) Reads that were too short: 18,969 (0.5%) Reads written (passing filters): 261,093 (7.5%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 67,450,601 bp (6.5%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 280062 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 899 847.3 0 899 7 1490 847.3 0 1490 8 63260 847.3 0 63260 9 73306 847.3 0 73306 10 129 847.3 0 129 11 58 847.3 0 58 12 49 847.3 0 49 13 60 847.3 0 60 14 45 847.3 0 45 15 35 847.3 0 35 16 72 847.3 0 72 17 63 847.3 0 63 18 69 847.3 0 69 19 76 847.3 0 76 20 51 847.3 0 51 21 23 847.3 0 23 22 48 847.3 0 48 23 42 847.3 0 42 24 63 847.3 0 63 25 49 847.3 0 49 26 57 847.3 0 57 27 49 847.3 0 49 28 41 847.3 0 41 29 47 847.3 0 47 30 75 847.3 0 75 31 57 847.3 0 57 32 88 847.3 0 88 33 153 847.3 0 153 34 76 847.3 0 76 35 103 847.3 0 103 36 101 847.3 0 101 37 80 847.3 0 80 38 56 847.3 0 56 39 106 847.3 0 106 40 55 847.3 0 55 41 831 847.3 0 831 42 851 847.3 0 851 43 116 847.3 0 116 44 60 847.3 0 60 45 51 847.3 0 51 46 56 847.3 0 56 47 57 847.3 0 57 48 54 847.3 0 54 49 202 847.3 0 202 50 124 847.3 0 124 51 275 847.3 0 275 52 443 847.3 0 443 53 93 847.3 0 93 54 176 847.3 0 176 55 115 847.3 0 115 56 213 847.3 0 213 57 420 847.3 0 420 58 403 847.3 0 403 59 382 847.3 0 382 60 91 847.3 0 91 61 103 847.3 0 103 62 218 847.3 0 218 63 203 847.3 0 203 64 336 847.3 0 336 65 654 847.3 0 654 66 641 847.3 0 641 67 1642 847.3 0 1642 68 2927 847.3 0 2927 69 52680 847.3 0 52680 70 12991 847.3 0 12991 71 19897 847.3 0 19897 72 112 847.3 0 112 73 55 847.3 0 55 74 48 847.3 0 48 75 54 847.3 0 54 76 64 847.3 0 64 77 57 847.3 0 57 78 72 847.3 0 72 79 54 847.3 0 54 80 256 847.3 0 256 81 257 847.3 0 257 82 442 847.3 0 442 83 100 847.3 0 100 84 78 847.3 0 78 85 77 847.3 0 77 86 127 847.3 0 127 87 102 847.3 0 102 88 116 847.3 0 116 89 85 847.3 0 85 90 126 847.3 0 126 91 100 847.3 0 100 92 77 847.3 0 77 93 131 847.3 0 131 94 116 847.3 0 116 95 114 847.3 0 114 96 111 847.3 0 111 97 125 847.3 0 125 98 96 847.3 0 96 99 97 847.3 0 97 100 381 847.3 0 381 101 505 847.3 0 505 102 604 847.3 0 604 103 121 847.3 0 121 104 117 847.3 0 117 105 98 847.3 0 98 106 68 847.3 0 68 107 88 847.3 0 88 108 78 847.3 0 78 109 75 847.3 0 75 110 99 847.3 0 99 111 116 847.3 0 116 112 94 847.3 0 94 113 75 847.3 0 75 114 114 847.3 0 114 115 106 847.3 0 106 116 111 847.3 0 111 117 126 847.3 0 126 118 144 847.3 0 144 119 130 847.3 0 130 120 112 847.3 0 112 121 98 847.3 0 98 122 126 847.3 0 126 123 130 847.3 0 130 124 128 847.3 0 128 125 95 847.3 0 95 126 129 847.3 0 129 127 98 847.3 0 98 128 101 847.3 0 101 129 75 847.3 0 75 130 120 847.3 0 120 131 205 847.3 0 205 132 733 847.3 0 733 133 500 847.3 0 500 134 285 847.3 0 285 135 143 847.3 0 143 136 445 847.3 0 445 137 411 847.3 0 411 138 288 847.3 0 288 139 173 847.3 0 173 140 125 847.3 0 125 141 124 847.3 0 124 142 119 847.3 0 119 143 115 847.3 0 115 144 127 847.3 0 127 145 130 847.3 0 130 146 123 847.3 0 123 147 110 847.3 0 110 148 108 847.3 0 108 149 120 847.3 0 120 150 123 847.3 0 123 151 140 847.3 0 140 152 155 847.3 0 155 153 125 847.3 0 125 154 128 847.3 0 128 155 109 847.3 0 109 156 121 847.3 0 121 157 182 847.3 0 182 158 203 847.3 0 203 159 237 847.3 0 237 160 227 847.3 0 227 161 213 847.3 0 213 162 341 847.3 0 341 163 134 847.3 0 134 164 143 847.3 0 143 165 118 847.3 0 118 166 317 847.3 0 317 167 242 847.3 0 242 168 381 847.3 0 381 169 186 847.3 0 186 170 166 847.3 0 166 171 135 847.3 0 135 172 169 847.3 0 169 173 135 847.3 0 135 174 108 847.3 0 108 175 116 847.3 0 116 176 192 847.3 0 192 177 579 847.3 0 579 178 641 847.3 0 641 179 545 847.3 0 545 180 187 847.3 0 187 181 176 847.3 0 176 182 274 847.3 0 274 183 375 847.3 0 375 184 524 847.3 0 524 185 217 847.3 0 217 186 146 847.3 0 146 187 151 847.3 0 151 188 163 847.3 0 163 189 192 847.3 0 192 190 161 847.3 0 161 191 148 847.3 0 148 192 136 847.3 0 136 193 122 847.3 0 122 194 118 847.3 0 118 195 107 847.3 0 107 196 141 847.3 0 141 197 139 847.3 0 139 198 180 847.3 0 180 199 140 847.3 0 140 200 122 847.3 0 122 201 132 847.3 0 132 202 390 847.3 0 390 203 469 847.3 0 469 204 335 847.3 0 335 205 188 847.3 0 188 206 97 847.3 0 97 207 118 847.3 0 118 208 105 847.3 0 105 209 121 847.3 0 121 210 140 847.3 0 140 211 126 847.3 0 126 212 161 847.3 0 161 213 192 847.3 0 192 214 153 847.3 0 153 215 123 847.3 0 123 216 117 847.3 0 117 217 149 847.3 0 149 218 136 847.3 0 136 219 113 847.3 0 113 220 115 847.3 0 115 221 113 847.3 0 113 222 186 847.3 0 186 223 382 847.3 0 382 224 358 847.3 0 358 225 327 847.3 0 327 226 182 847.3 0 182 227 161 847.3 0 161 228 207 847.3 0 207 229 207 847.3 0 207 230 110 847.3 0 110 231 115 847.3 0 115 232 129 847.3 0 129 233 131 847.3 0 131 234 131 847.3 0 131 235 107 847.3 0 107 236 98 847.3 0 98 237 322 847.3 0 322 238 123 847.3 0 123 239 153 847.3 0 153 240 154 847.3 0 154 241 103 847.3 0 103 242 253 847.3 0 253 243 266 847.3 0 266 244 243 847.3 0 243 245 192 847.3 0 192 246 177 847.3 0 177 247 119 847.3 0 119 248 111 847.3 0 111 249 117 847.3 0 117 250 136 847.3 0 136 251 315 847.3 0 315 252 197 847.3 0 197 253 156 847.3 0 156 254 114 847.3 0 114 255 140 847.3 0 140 256 208 847.3 0 208 257 202 847.3 0 202 258 185 847.3 0 185 259 120 847.3 0 120 260 147 847.3 0 147 261 134 847.3 0 134 262 151 847.3 0 151 263 189 847.3 0 189 264 171 847.3 0 171 265 233 847.3 0 233 266 234 847.3 0 234 267 183 847.3 0 183 268 117 847.3 0 117 269 553 847.3 0 553 270 465 847.3 0 465 271 311 847.3 0 311 272 296 847.3 0 296 273 278 847.3 0 278 274 208 847.3 0 208 275 161 847.3 0 161 276 186 847.3 0 186 277 274 847.3 0 274 278 233 847.3 0 233 279 181 847.3 0 181 280 107 847.3 0 107 281 145 847.3 0 145 282 136 847.3 0 136 283 191 847.3 0 191 284 195 847.3 0 195 285 194 847.3 0 194 286 191 847.3 0 191 287 168 847.3 0 168 288 133 847.3 0 133 289 128 847.3 0 128 290 133 847.3 0 133 291 232 847.3 0 232 292 298 847.3 0 298 293 201 847.3 0 201 294 159 847.3 0 159 295 111 847.3 0 111 296 124 847.3 0 124 297 140 847.3 0 140 298 181 847.3 0 181 299 240 847.3 0 240 300 254 847.3 0 254 301 205 847.3 0 205 Finished in 18.32 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 261,093 Reads with adapters: 62,829 (24.1%) Reads that were too short: 50 (0.0%) Reads written (passing filters): 62,779 (24.0%) Total basepairs processed: 67,450,601 bp Total written (filtered): 17,314,235 bp (25.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 62829 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 24 0.0 3 0 0 16 8 16 456 0.0 3 0 447 9 17 61954 0.0 3 60708 1190 47 9 18 165 0.0 3 10 151 3 1 19 25 0.0 3 0 0 21 4 20 67 0.0 3 0 0 0 67 23 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 32 2 0.0 3 1 0 0 1 34 6 0.0 3 0 0 0 6 35 1 0.0 3 1 36 1 0.0 3 1 37 6 0.0 3 0 0 0 6 40 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 55 17 0.0 3 0 0 1 16 58 2 0.0 3 0 0 0 2 59 3 0.0 3 0 0 0 3 60 4 0.0 3 0 0 0 4 65 3 0.0 3 1 0 0 2 66 1 0.0 3 0 0 0 1 71 3 0.0 3 0 0 0 3 79 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 18 0.0 3 0 0 0 18 93 1 0.0 3 0 0 0 1 94 3 0.0 3 0 0 0 3 96 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 120 3 0.0 3 0 0 0 3 130 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 144 2 0.0 3 1 0 0 1 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 185 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 194 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 0 2 218 1 0.0 3 0 0 0 1 219 3 0.0 3 0 0 0 3 220 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 4 0.0 3 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.77 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 62,779 Reads with adapters: 6,303 (10.0%) Reads written (passing filters): 62,779 (100.0%) Total basepairs processed: 17,314,235 bp Total written (filtered): 17,295,189 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 6303 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 91.4% C: 5.1% G: 2.8% T: 0.7% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6175 980.9 0 6175 4 122 245.2 0 122 5 3 61.3 0 3 6 3 15.3 0 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.