This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 161.78 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 72,286 (2.1%) Reads that were too short: 16,565 (0.5%) Reads written (passing filters): 55,721 (1.6%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 13,936,629 bp (1.3%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 72286 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1486 847.3 0 1486 7 13147 847.3 0 13147 8 537 847.3 0 537 9 15147 847.3 0 15147 10 115 847.3 0 115 11 96 847.3 0 96 12 71 847.3 0 71 13 76 847.3 0 76 14 86 847.3 0 86 15 91 847.3 0 91 16 96 847.3 0 96 17 82 847.3 0 82 18 79 847.3 0 79 19 80 847.3 0 80 20 170 847.3 0 170 21 93 847.3 0 93 22 119 847.3 0 119 23 389 847.3 0 389 24 534 847.3 0 534 25 1052 847.3 0 1052 26 51 847.3 0 51 27 41 847.3 0 41 28 69 847.3 0 69 29 68 847.3 0 68 30 66 847.3 0 66 31 54 847.3 0 54 32 66 847.3 0 66 33 64 847.3 0 64 34 82 847.3 0 82 35 83 847.3 0 83 36 62 847.3 0 62 37 92 847.3 0 92 38 63 847.3 0 63 39 72 847.3 0 72 40 58 847.3 0 58 41 65 847.3 0 65 42 56 847.3 0 56 43 63 847.3 0 63 44 62 847.3 0 62 45 65 847.3 0 65 46 71 847.3 0 71 47 63 847.3 0 63 48 59 847.3 0 59 49 66 847.3 0 66 50 59 847.3 0 59 51 57 847.3 0 57 52 58 847.3 0 58 53 46 847.3 0 46 54 67 847.3 0 67 55 60 847.3 0 60 56 70 847.3 0 70 57 58 847.3 0 58 58 63 847.3 0 63 59 58 847.3 0 58 60 64 847.3 0 64 61 62 847.3 0 62 62 50 847.3 0 50 63 44 847.3 0 44 64 67 847.3 0 67 65 69 847.3 0 69 66 69 847.3 0 69 67 55 847.3 0 55 68 45 847.3 0 45 69 62 847.3 0 62 70 79 847.3 0 79 71 74 847.3 0 74 72 66 847.3 0 66 73 66 847.3 0 66 74 108 847.3 0 108 75 153 847.3 0 153 76 51 847.3 0 51 77 53 847.3 0 53 78 55 847.3 0 55 79 33 847.3 0 33 80 45 847.3 0 45 81 65 847.3 0 65 82 75 847.3 0 75 83 70 847.3 0 70 84 104 847.3 0 104 85 122 847.3 0 122 86 102 847.3 0 102 87 150 847.3 0 150 88 664 847.3 0 664 89 547 847.3 0 547 90 491 847.3 0 491 91 40 847.3 0 40 92 53 847.3 0 53 93 67 847.3 0 67 94 88 847.3 0 88 95 123 847.3 0 123 96 404 847.3 0 404 97 234 847.3 0 234 98 113 847.3 0 113 99 71 847.3 0 71 100 120 847.3 0 120 101 515 847.3 0 515 102 1026 847.3 0 1026 103 1515 847.3 0 1515 104 109 847.3 0 109 105 69 847.3 0 69 106 56 847.3 0 56 107 34 847.3 0 34 108 38 847.3 0 38 109 52 847.3 0 52 110 87 847.3 0 87 111 90 847.3 0 90 112 75 847.3 0 75 113 144 847.3 0 144 114 155 847.3 0 155 115 88 847.3 0 88 116 75 847.3 0 75 117 102 847.3 0 102 118 72 847.3 0 72 119 66 847.3 0 66 120 88 847.3 0 88 121 66 847.3 0 66 122 93 847.3 0 93 123 92 847.3 0 92 124 131 847.3 0 131 125 116 847.3 0 116 126 256 847.3 0 256 127 2280 847.3 0 2280 128 908 847.3 0 908 129 1363 847.3 0 1363 130 112 847.3 0 112 131 89 847.3 0 89 132 85 847.3 0 85 133 85 847.3 0 85 134 88 847.3 0 88 135 75 847.3 0 75 136 54 847.3 0 54 137 86 847.3 0 86 138 68 847.3 0 68 139 67 847.3 0 67 140 66 847.3 0 66 141 101 847.3 0 101 142 68 847.3 0 68 143 70 847.3 0 70 144 176 847.3 0 176 145 77 847.3 0 77 146 126 847.3 0 126 147 80 847.3 0 80 148 61 847.3 0 61 149 97 847.3 0 97 150 53 847.3 0 53 151 55 847.3 0 55 152 71 847.3 0 71 153 50 847.3 0 50 154 49 847.3 0 49 155 54 847.3 0 54 156 71 847.3 0 71 157 66 847.3 0 66 158 57 847.3 0 57 159 56 847.3 0 56 160 52 847.3 0 52 161 78 847.3 0 78 162 57 847.3 0 57 163 58 847.3 0 58 164 43 847.3 0 43 165 36 847.3 0 36 166 47 847.3 0 47 167 56 847.3 0 56 168 59 847.3 0 59 169 85 847.3 0 85 170 52 847.3 0 52 171 51 847.3 0 51 172 65 847.3 0 65 173 33 847.3 0 33 174 39 847.3 0 39 175 50 847.3 0 50 176 113 847.3 0 113 177 92 847.3 0 92 178 101 847.3 0 101 179 43 847.3 0 43 180 48 847.3 0 48 181 60 847.3 0 60 182 84 847.3 0 84 183 74 847.3 0 74 184 52 847.3 0 52 185 55 847.3 0 55 186 47 847.3 0 47 187 59 847.3 0 59 188 46 847.3 0 46 189 50 847.3 0 50 190 50 847.3 0 50 191 40 847.3 0 40 192 56 847.3 0 56 193 98 847.3 0 98 194 103 847.3 0 103 195 114 847.3 0 114 196 128 847.3 0 128 197 90 847.3 0 90 198 73 847.3 0 73 199 42 847.3 0 42 200 138 847.3 0 138 201 154 847.3 0 154 202 159 847.3 0 159 203 98 847.3 0 98 204 65 847.3 0 65 205 64 847.3 0 64 206 56 847.3 0 56 207 72 847.3 0 72 208 117 847.3 0 117 209 133 847.3 0 133 210 91 847.3 0 91 211 79 847.3 0 79 212 61 847.3 0 61 213 80 847.3 0 80 214 63 847.3 0 63 215 79 847.3 0 79 216 67 847.3 0 67 217 83 847.3 0 83 218 104 847.3 0 104 219 84 847.3 0 84 220 62 847.3 0 62 221 68 847.3 0 68 222 61 847.3 0 61 223 100 847.3 0 100 224 66 847.3 0 66 225 54 847.3 0 54 226 64 847.3 0 64 227 58 847.3 0 58 228 38 847.3 0 38 229 80 847.3 0 80 230 79 847.3 0 79 231 74 847.3 0 74 232 83 847.3 0 83 233 60 847.3 0 60 234 184 847.3 0 184 235 216 847.3 0 216 236 88 847.3 0 88 237 79 847.3 0 79 238 122 847.3 0 122 239 145 847.3 0 145 240 250 847.3 0 250 241 205 847.3 0 205 242 184 847.3 0 184 243 98 847.3 0 98 244 64 847.3 0 64 245 91 847.3 0 91 246 90 847.3 0 90 247 86 847.3 0 86 248 57 847.3 0 57 249 89 847.3 0 89 250 80 847.3 0 80 251 65 847.3 0 65 252 91 847.3 0 91 253 61 847.3 0 61 254 101 847.3 0 101 255 303 847.3 0 303 256 107 847.3 0 107 257 120 847.3 0 120 258 151 847.3 0 151 259 1643 847.3 0 1643 260 2185 847.3 0 2185 261 1549 847.3 0 1549 262 304 847.3 0 304 263 65 847.3 0 65 264 59 847.3 0 59 265 43 847.3 0 43 266 57 847.3 0 57 267 63 847.3 0 63 268 81 847.3 0 81 269 70 847.3 0 70 270 55 847.3 0 55 271 49 847.3 0 49 272 52 847.3 0 52 273 79 847.3 0 79 274 59 847.3 0 59 275 69 847.3 0 69 276 75 847.3 0 75 277 72 847.3 0 72 278 94 847.3 0 94 279 743 847.3 0 743 280 1494 847.3 0 1494 281 208 847.3 0 208 282 79 847.3 0 79 283 62 847.3 0 62 284 74 847.3 0 74 285 53 847.3 0 53 286 71 847.3 0 71 287 54 847.3 0 54 288 59 847.3 0 59 289 70 847.3 0 70 290 54 847.3 0 54 291 96 847.3 0 96 292 124 847.3 0 124 293 140 847.3 0 140 294 125 847.3 0 125 295 125 847.3 0 125 296 110 847.3 0 110 297 121 847.3 0 121 298 74 847.3 0 74 299 101 847.3 0 101 300 87 847.3 0 87 301 89 847.3 0 89 Finished in 4.17 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 55,721 Reads with adapters: 14,598 (26.2%) Reads that were too short: 31 (0.1%) Reads written (passing filters): 14,567 (26.1%) Total basepairs processed: 13,936,629 bp Total written (filtered): 3,949,435 bp (28.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14598 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 12 0.0 3 0 0 8 3 1 19 179 0.0 3 0 156 16 5 2 20 12841 0.0 4 12305 442 65 23 6 21 37 0.0 4 6 30 0 0 1 22 37 0.0 4 11 18 5 3 23 1084 0.0 4 998 55 8 18 5 24 5 0.0 4 3 2 25 2 0.0 4 0 0 0 0 2 26 4 0.0 4 0 0 0 3 1 27 1 0.0 4 0 0 0 1 38 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 126 10 0.0 4 0 0 0 0 10 127 345 0.0 4 0 0 0 0 345 128 3 0.0 4 0 0 0 0 3 130 1 0.0 4 0 0 0 0 1 148 2 0.0 4 0 0 0 0 2 153 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 1 195 1 0.0 4 1 203 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 2 0.0 4 0 1 0 0 1 230 2 0.0 4 0 1 0 0 1 256 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.85 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 14,567 Reads with adapters: 1,174 (8.1%) Reads written (passing filters): 14,567 (100.0%) Total basepairs processed: 3,949,435 bp Total written (filtered): 3,924,361 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1174 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.9% C: 8.1% G: 81.6% T: 5.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 54 227.6 0 54 4 9 56.9 0 9 5 1 14.2 0 1 9 243 0.1 0 15 228 10 138 0.0 1 6 132 11 550 0.0 1 14 536 12 109 0.0 1 2 107 13 4 0.0 1 0 4 15 2 0.0 1 0 2 152 7 0.0 1 5 2 210 7 0.0 1 6 1 223 1 0.0 1 1 225 45 0.0 1 42 3 226 2 0.0 1 1 1 265 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.