This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 156.31 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 71,940 (2.1%) Reads that were too short: 13,073 (0.4%) Reads written (passing filters): 58,867 (1.7%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 15,513,368 bp (1.5%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 71940 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 358 847.3 0 358 7 768 847.3 0 768 8 24420 847.3 0 24420 9 16644 847.3 0 16644 10 64 847.3 0 64 11 91 847.3 0 91 12 16 847.3 0 16 13 32 847.3 0 32 14 46 847.3 0 46 15 72 847.3 0 72 16 39 847.3 0 39 17 42 847.3 0 42 18 58 847.3 0 58 19 76 847.3 0 76 20 107 847.3 0 107 21 22 847.3 0 22 22 16 847.3 0 16 23 33 847.3 0 33 24 38 847.3 0 38 25 25 847.3 0 25 26 16 847.3 0 16 27 39 847.3 0 39 28 109 847.3 0 109 29 183 847.3 0 183 30 65 847.3 0 65 31 27 847.3 0 27 32 29 847.3 0 29 33 19 847.3 0 19 34 31 847.3 0 31 35 38 847.3 0 38 36 45 847.3 0 45 37 37 847.3 0 37 38 25 847.3 0 25 39 18 847.3 0 18 40 33 847.3 0 33 41 28 847.3 0 28 42 24 847.3 0 24 43 60 847.3 0 60 44 118 847.3 0 118 45 595 847.3 0 595 46 496 847.3 0 496 47 189 847.3 0 189 48 17 847.3 0 17 49 19 847.3 0 19 50 18 847.3 0 18 51 18 847.3 0 18 52 19 847.3 0 19 53 26 847.3 0 26 54 16 847.3 0 16 55 16 847.3 0 16 56 19 847.3 0 19 57 15 847.3 0 15 58 23 847.3 0 23 59 15 847.3 0 15 60 30 847.3 0 30 61 15 847.3 0 15 62 17 847.3 0 17 63 17 847.3 0 17 64 22 847.3 0 22 65 19 847.3 0 19 66 16 847.3 0 16 67 15 847.3 0 15 68 25 847.3 0 25 69 101 847.3 0 101 70 44 847.3 0 44 71 102 847.3 0 102 72 133 847.3 0 133 73 129 847.3 0 129 74 116 847.3 0 116 75 58 847.3 0 58 76 39 847.3 0 39 77 33 847.3 0 33 78 75 847.3 0 75 79 103 847.3 0 103 80 504 847.3 0 504 81 172 847.3 0 172 82 99 847.3 0 99 83 153 847.3 0 153 84 195 847.3 0 195 85 77 847.3 0 77 86 44 847.3 0 44 87 21 847.3 0 21 88 36 847.3 0 36 89 29 847.3 0 29 90 41 847.3 0 41 91 39 847.3 0 39 92 39 847.3 0 39 93 41 847.3 0 41 94 178 847.3 0 178 95 259 847.3 0 259 96 786 847.3 0 786 97 478 847.3 0 478 98 28 847.3 0 28 99 34 847.3 0 34 100 22 847.3 0 22 101 22 847.3 0 22 102 23 847.3 0 23 103 35 847.3 0 35 104 25 847.3 0 25 105 15 847.3 0 15 106 26 847.3 0 26 107 31 847.3 0 31 108 37 847.3 0 37 109 32 847.3 0 32 110 28 847.3 0 28 111 43 847.3 0 43 112 65 847.3 0 65 113 591 847.3 0 591 114 439 847.3 0 439 115 213 847.3 0 213 116 125 847.3 0 125 117 54 847.3 0 54 118 31 847.3 0 31 119 27 847.3 0 27 120 52 847.3 0 52 121 46 847.3 0 46 122 45 847.3 0 45 123 69 847.3 0 69 124 109 847.3 0 109 125 41 847.3 0 41 126 52 847.3 0 52 127 45 847.3 0 45 128 59 847.3 0 59 129 60 847.3 0 60 130 62 847.3 0 62 131 72 847.3 0 72 132 67 847.3 0 67 133 94 847.3 0 94 134 55 847.3 0 55 135 156 847.3 0 156 136 257 847.3 0 257 137 487 847.3 0 487 138 251 847.3 0 251 139 138 847.3 0 138 140 225 847.3 0 225 141 73 847.3 0 73 142 124 847.3 0 124 143 138 847.3 0 138 144 89 847.3 0 89 145 70 847.3 0 70 146 36 847.3 0 36 147 49 847.3 0 49 148 37 847.3 0 37 149 66 847.3 0 66 150 62 847.3 0 62 151 32 847.3 0 32 152 36 847.3 0 36 153 33 847.3 0 33 154 42 847.3 0 42 155 52 847.3 0 52 156 55 847.3 0 55 157 45 847.3 0 45 158 39 847.3 0 39 159 30 847.3 0 30 160 29 847.3 0 29 161 31 847.3 0 31 162 70 847.3 0 70 163 84 847.3 0 84 164 100 847.3 0 100 165 60 847.3 0 60 166 53 847.3 0 53 167 104 847.3 0 104 168 206 847.3 0 206 169 123 847.3 0 123 170 78 847.3 0 78 171 49 847.3 0 49 172 34 847.3 0 34 173 52 847.3 0 52 174 50 847.3 0 50 175 35 847.3 0 35 176 30 847.3 0 30 177 30 847.3 0 30 178 53 847.3 0 53 179 138 847.3 0 138 180 85 847.3 0 85 181 56 847.3 0 56 182 53 847.3 0 53 183 98 847.3 0 98 184 251 847.3 0 251 185 426 847.3 0 426 186 344 847.3 0 344 187 178 847.3 0 178 188 72 847.3 0 72 189 67 847.3 0 67 190 59 847.3 0 59 191 66 847.3 0 66 192 35 847.3 0 35 193 58 847.3 0 58 194 40 847.3 0 40 195 47 847.3 0 47 196 41 847.3 0 41 197 37 847.3 0 37 198 42 847.3 0 42 199 85 847.3 0 85 200 56 847.3 0 56 201 54 847.3 0 54 202 48 847.3 0 48 203 93 847.3 0 93 204 401 847.3 0 401 205 481 847.3 0 481 206 319 847.3 0 319 207 126 847.3 0 126 208 84 847.3 0 84 209 110 847.3 0 110 210 76 847.3 0 76 211 66 847.3 0 66 212 94 847.3 0 94 213 60 847.3 0 60 214 51 847.3 0 51 215 101 847.3 0 101 216 80 847.3 0 80 217 78 847.3 0 78 218 68 847.3 0 68 219 70 847.3 0 70 220 62 847.3 0 62 221 64 847.3 0 64 222 82 847.3 0 82 223 121 847.3 0 121 224 314 847.3 0 314 225 1835 847.3 0 1835 226 2521 847.3 0 2521 227 1176 847.3 0 1176 228 267 847.3 0 267 229 41 847.3 0 41 230 42 847.3 0 42 231 38 847.3 0 38 232 50 847.3 0 50 233 44 847.3 0 44 234 82 847.3 0 82 235 130 847.3 0 130 236 134 847.3 0 134 237 54 847.3 0 54 238 40 847.3 0 40 239 103 847.3 0 103 240 89 847.3 0 89 241 48 847.3 0 48 242 42 847.3 0 42 243 44 847.3 0 44 244 62 847.3 0 62 245 40 847.3 0 40 246 53 847.3 0 53 247 46 847.3 0 46 248 57 847.3 0 57 249 45 847.3 0 45 250 31 847.3 0 31 251 23 847.3 0 23 252 30 847.3 0 30 253 47 847.3 0 47 254 57 847.3 0 57 255 49 847.3 0 49 256 26 847.3 0 26 257 50 847.3 0 50 258 44 847.3 0 44 259 53 847.3 0 53 260 53 847.3 0 53 261 45 847.3 0 45 262 43 847.3 0 43 263 51 847.3 0 51 264 202 847.3 0 202 265 144 847.3 0 144 266 80 847.3 0 80 267 15 847.3 0 15 268 31 847.3 0 31 269 57 847.3 0 57 270 34 847.3 0 34 271 28 847.3 0 28 272 32 847.3 0 32 273 47 847.3 0 47 274 46 847.3 0 46 275 56 847.3 0 56 276 38 847.3 0 38 277 44 847.3 0 44 278 25 847.3 0 25 279 59 847.3 0 59 280 89 847.3 0 89 281 49 847.3 0 49 282 41 847.3 0 41 283 32 847.3 0 32 284 29 847.3 0 29 285 31 847.3 0 31 286 44 847.3 0 44 287 32 847.3 0 32 288 36 847.3 0 36 289 37 847.3 0 37 290 53 847.3 0 53 291 48 847.3 0 48 292 52 847.3 0 52 293 79 847.3 0 79 294 86 847.3 0 86 295 130 847.3 0 130 296 62 847.3 0 62 297 103 847.3 0 103 298 77 847.3 0 77 299 65 847.3 0 65 300 41 847.3 0 41 301 185 847.3 0 185 Finished in 4.36 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 58,867 Reads with adapters: 17,401 (29.6%) Reads that were too short: 36 (0.1%) Reads written (passing filters): 17,365 (29.5%) Total basepairs processed: 15,513,368 bp Total written (filtered): 4,712,503 bp (30.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 17401 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 12 0.0 3 0 0 11 1 19 211 0.0 3 0 187 11 8 5 20 16907 0.0 4 16035 696 129 32 15 21 46 0.0 4 14 26 6 22 7 0.0 4 0 0 5 2 23 65 0.0 4 0 0 0 33 32 24 2 0.0 4 1 0 0 0 1 27 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 47 4 0.0 4 0 0 0 0 4 51 2 0.0 4 0 0 0 1 1 56 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 71 1 0.0 4 1 73 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 89 8 0.0 4 0 0 0 0 8 94 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 82 0.0 4 0 0 0 0 82 134 1 0.0 4 0 0 0 0 1 141 2 0.0 4 1 0 0 0 1 146 1 0.0 4 0 0 0 0 1 153 2 0.0 4 1 0 0 0 1 155 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 172 2 0.0 4 0 0 0 0 2 192 1 0.0 4 0 0 1 197 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 227 2 0.0 4 0 0 0 0 2 228 5 0.0 4 0 0 0 0 5 229 4 0.0 4 0 0 0 0 4 235 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 1 246 1 0.0 4 0 0 0 0 1 248 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 260 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 273 1 0.0 4 1 280 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.06 s (61 us/read; 0.98 M reads/minute). === Summary === Total reads processed: 17,365 Reads with adapters: 4,177 (24.1%) Reads written (passing filters): 17,365 (100.0%) Total basepairs processed: 4,712,503 bp Total written (filtered): 4,658,067 bp (98.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4177 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.7% C: 5.1% G: 90.5% T: 2.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 14 271.3 0 14 4 13 67.8 0 13 6 31 4.2 0 31 7 1 1.1 0 1 8 2 0.3 0 2 9 1410 0.1 0 96 1314 10 2245 0.0 1 138 2107 11 377 0.0 1 5 372 12 10 0.0 1 0 10 13 12 0.0 1 0 12 150 4 0.0 1 4 151 1 0.0 1 1 189 1 0.0 1 0 1 208 11 0.0 1 10 1 209 1 0.0 1 1 223 14 0.0 1 12 2 224 2 0.0 1 2 268 1 0.0 1 1 270 23 0.0 1 23 271 3 0.0 1 2 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.