This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 160.72 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 65,164 (1.9%) Reads that were too short: 10,221 (0.3%) Reads written (passing filters): 54,943 (1.6%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 14,775,603 bp (1.4%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 65164 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 220 847.3 0 220 7 431 847.3 0 431 8 22643 847.3 0 22643 9 19521 847.3 0 19521 10 39 847.3 0 39 11 44 847.3 0 44 12 8 847.3 0 8 13 22 847.3 0 22 14 9 847.3 0 9 15 12 847.3 0 12 16 13 847.3 0 13 17 21 847.3 0 21 18 24 847.3 0 24 19 30 847.3 0 30 20 50 847.3 0 50 21 15 847.3 0 15 22 14 847.3 0 14 23 15 847.3 0 15 24 14 847.3 0 14 25 14 847.3 0 14 26 15 847.3 0 15 27 7 847.3 0 7 28 28 847.3 0 28 29 34 847.3 0 34 30 32 847.3 0 32 31 16 847.3 0 16 32 17 847.3 0 17 33 14 847.3 0 14 34 28 847.3 0 28 35 46 847.3 0 46 36 25 847.3 0 25 37 27 847.3 0 27 38 11 847.3 0 11 39 22 847.3 0 22 40 13 847.3 0 13 41 16 847.3 0 16 42 19 847.3 0 19 43 39 847.3 0 39 44 57 847.3 0 57 45 457 847.3 0 457 46 303 847.3 0 303 47 128 847.3 0 128 48 19 847.3 0 19 49 11 847.3 0 11 50 17 847.3 0 17 51 17 847.3 0 17 52 19 847.3 0 19 53 12 847.3 0 12 54 18 847.3 0 18 55 16 847.3 0 16 56 17 847.3 0 17 57 21 847.3 0 21 58 18 847.3 0 18 59 20 847.3 0 20 60 16 847.3 0 16 61 12 847.3 0 12 62 14 847.3 0 14 63 12 847.3 0 12 64 17 847.3 0 17 65 16 847.3 0 16 66 15 847.3 0 15 67 17 847.3 0 17 68 21 847.3 0 21 69 80 847.3 0 80 70 14 847.3 0 14 71 36 847.3 0 36 72 42 847.3 0 42 73 68 847.3 0 68 74 11 847.3 0 11 75 13 847.3 0 13 76 57 847.3 0 57 77 84 847.3 0 84 78 58 847.3 0 58 79 47 847.3 0 47 80 50 847.3 0 50 81 50 847.3 0 50 82 40 847.3 0 40 83 25 847.3 0 25 84 43 847.3 0 43 85 66 847.3 0 66 86 44 847.3 0 44 87 41 847.3 0 41 88 64 847.3 0 64 89 43 847.3 0 43 90 49 847.3 0 49 91 71 847.3 0 71 92 69 847.3 0 69 93 66 847.3 0 66 94 205 847.3 0 205 95 267 847.3 0 267 96 813 847.3 0 813 97 626 847.3 0 626 98 39 847.3 0 39 99 32 847.3 0 32 100 35 847.3 0 35 101 30 847.3 0 30 102 22 847.3 0 22 103 14 847.3 0 14 104 23 847.3 0 23 105 15 847.3 0 15 106 29 847.3 0 29 107 36 847.3 0 36 108 46 847.3 0 46 109 28 847.3 0 28 110 26 847.3 0 26 111 31 847.3 0 31 112 51 847.3 0 51 113 314 847.3 0 314 114 253 847.3 0 253 115 141 847.3 0 141 116 105 847.3 0 105 117 35 847.3 0 35 118 19 847.3 0 19 119 36 847.3 0 36 120 42 847.3 0 42 121 33 847.3 0 33 122 39 847.3 0 39 123 54 847.3 0 54 124 79 847.3 0 79 125 35 847.3 0 35 126 32 847.3 0 32 127 39 847.3 0 39 128 41 847.3 0 41 129 54 847.3 0 54 130 44 847.3 0 44 131 54 847.3 0 54 132 43 847.3 0 43 133 81 847.3 0 81 134 48 847.3 0 48 135 173 847.3 0 173 136 249 847.3 0 249 137 402 847.3 0 402 138 215 847.3 0 215 139 104 847.3 0 104 140 191 847.3 0 191 141 47 847.3 0 47 142 111 847.3 0 111 143 114 847.3 0 114 144 56 847.3 0 56 145 53 847.3 0 53 146 40 847.3 0 40 147 30 847.3 0 30 148 29 847.3 0 29 149 47 847.3 0 47 150 38 847.3 0 38 151 29 847.3 0 29 152 41 847.3 0 41 153 30 847.3 0 30 154 44 847.3 0 44 155 42 847.3 0 42 156 39 847.3 0 39 157 40 847.3 0 40 158 35 847.3 0 35 159 25 847.3 0 25 160 24 847.3 0 24 161 29 847.3 0 29 162 55 847.3 0 55 163 81 847.3 0 81 164 73 847.3 0 73 165 42 847.3 0 42 166 30 847.3 0 30 167 105 847.3 0 105 168 189 847.3 0 189 169 81 847.3 0 81 170 41 847.3 0 41 171 35 847.3 0 35 172 39 847.3 0 39 173 41 847.3 0 41 174 39 847.3 0 39 175 26 847.3 0 26 176 30 847.3 0 30 177 28 847.3 0 28 178 46 847.3 0 46 179 92 847.3 0 92 180 61 847.3 0 61 181 44 847.3 0 44 182 45 847.3 0 45 183 57 847.3 0 57 184 165 847.3 0 165 185 243 847.3 0 243 186 207 847.3 0 207 187 103 847.3 0 103 188 45 847.3 0 45 189 45 847.3 0 45 190 50 847.3 0 50 191 48 847.3 0 48 192 31 847.3 0 31 193 37 847.3 0 37 194 38 847.3 0 38 195 43 847.3 0 43 196 32 847.3 0 32 197 40 847.3 0 40 198 50 847.3 0 50 199 54 847.3 0 54 200 41 847.3 0 41 201 36 847.3 0 36 202 34 847.3 0 34 203 64 847.3 0 64 204 196 847.3 0 196 205 207 847.3 0 207 206 130 847.3 0 130 207 69 847.3 0 69 208 56 847.3 0 56 209 72 847.3 0 72 210 64 847.3 0 64 211 50 847.3 0 50 212 51 847.3 0 51 213 61 847.3 0 61 214 34 847.3 0 34 215 70 847.3 0 70 216 89 847.3 0 89 217 67 847.3 0 67 218 62 847.3 0 62 219 43 847.3 0 43 220 50 847.3 0 50 221 61 847.3 0 61 222 59 847.3 0 59 223 113 847.3 0 113 224 282 847.3 0 282 225 1486 847.3 0 1486 226 2260 847.3 0 2260 227 999 847.3 0 999 228 280 847.3 0 280 229 30 847.3 0 30 230 29 847.3 0 29 231 40 847.3 0 40 232 38 847.3 0 38 233 42 847.3 0 42 234 46 847.3 0 46 235 75 847.3 0 75 236 74 847.3 0 74 237 39 847.3 0 39 238 20 847.3 0 20 239 48 847.3 0 48 240 50 847.3 0 50 241 20 847.3 0 20 242 57 847.3 0 57 243 42 847.3 0 42 244 37 847.3 0 37 245 38 847.3 0 38 246 38 847.3 0 38 247 41 847.3 0 41 248 49 847.3 0 49 249 43 847.3 0 43 250 35 847.3 0 35 251 26 847.3 0 26 252 26 847.3 0 26 253 55 847.3 0 55 254 49 847.3 0 49 255 39 847.3 0 39 256 37 847.3 0 37 257 40 847.3 0 40 258 36 847.3 0 36 259 50 847.3 0 50 260 48 847.3 0 48 261 43 847.3 0 43 262 26 847.3 0 26 263 40 847.3 0 40 264 89 847.3 0 89 265 73 847.3 0 73 266 45 847.3 0 45 267 18 847.3 0 18 268 35 847.3 0 35 269 50 847.3 0 50 270 29 847.3 0 29 271 23 847.3 0 23 272 29 847.3 0 29 273 39 847.3 0 39 274 41 847.3 0 41 275 30 847.3 0 30 276 23 847.3 0 23 277 29 847.3 0 29 278 28 847.3 0 28 279 41 847.3 0 41 280 70 847.3 0 70 281 51 847.3 0 51 282 22 847.3 0 22 283 38 847.3 0 38 284 43 847.3 0 43 285 29 847.3 0 29 286 43 847.3 0 43 287 31 847.3 0 31 288 37 847.3 0 37 289 39 847.3 0 39 290 33 847.3 0 33 291 40 847.3 0 40 292 36 847.3 0 36 293 62 847.3 0 62 294 64 847.3 0 64 295 158 847.3 0 158 296 45 847.3 0 45 297 53 847.3 0 53 298 56 847.3 0 56 299 45 847.3 0 45 300 24 847.3 0 24 301 125 847.3 0 125 Finished in 3.98 s (72 us/read; 0.83 M reads/minute). === Summary === Total reads processed: 54,943 Reads with adapters: 22,247 (40.5%) Reads that were too short: 31 (0.1%) Reads written (passing filters): 22,216 (40.4%) Total basepairs processed: 14,775,603 bp Total written (filtered): 6,125,392 bp (41.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 22247 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 145 0.0 3 0 139 5 1 17 21929 0.0 3 21538 382 9 18 64 0.0 3 15 46 3 19 15 0.0 3 0 0 13 2 20 19 0.0 3 0 0 1 18 24 2 0.0 3 0 0 0 2 28 1 0.0 3 0 0 0 1 31 1 0.0 3 1 42 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 55 2 0.0 3 0 0 0 2 56 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 1 75 1 0.0 3 0 0 0 1 78 16 0.0 3 0 0 0 16 81 4 0.0 3 0 0 0 4 90 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 148 1 0.0 3 1 149 2 0.0 3 0 1 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.26 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 22,216 Reads with adapters: 225 (1.0%) Reads written (passing filters): 22,216 (100.0%) Total basepairs processed: 6,125,392 bp Total written (filtered): 6,124,661 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 225 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 66.7% C: 8.9% G: 18.2% T: 6.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 174 347.1 0 174 4 46 86.8 0 46 5 5 21.7 0 5