This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 168.25 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 160,373 (4.6%) Reads that were too short: 18,259 (0.5%) Reads written (passing filters): 142,114 (4.1%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 34,556,903 bp (3.3%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 160373 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1938 847.3 0 1938 7 39120 847.3 0 39120 8 2745 847.3 0 2745 9 46189 847.3 0 46189 10 74 847.3 0 74 11 57 847.3 0 57 12 59 847.3 0 59 13 37 847.3 0 37 14 41 847.3 0 41 15 37 847.3 0 37 16 35 847.3 0 35 17 34 847.3 0 34 18 33 847.3 0 33 19 40 847.3 0 40 20 23 847.3 0 23 21 49 847.3 0 49 22 44 847.3 0 44 23 42 847.3 0 42 24 25 847.3 0 25 25 36 847.3 0 36 26 31 847.3 0 31 27 39 847.3 0 39 28 63 847.3 0 63 29 66 847.3 0 66 30 55 847.3 0 55 31 44 847.3 0 44 32 50 847.3 0 50 33 235 847.3 0 235 34 557 847.3 0 557 35 1337 847.3 0 1337 36 25 847.3 0 25 37 26 847.3 0 26 38 48 847.3 0 48 39 45 847.3 0 45 40 28 847.3 0 28 41 45 847.3 0 45 42 29 847.3 0 29 43 43 847.3 0 43 44 54 847.3 0 54 45 59 847.3 0 59 46 48 847.3 0 48 47 69 847.3 0 69 48 36 847.3 0 36 49 40 847.3 0 40 50 56 847.3 0 56 51 44 847.3 0 44 52 23 847.3 0 23 53 42 847.3 0 42 54 51 847.3 0 51 55 45 847.3 0 45 56 83 847.3 0 83 57 466 847.3 0 466 58 405 847.3 0 405 59 84 847.3 0 84 60 54 847.3 0 54 61 33 847.3 0 33 62 45 847.3 0 45 63 34 847.3 0 34 64 56 847.3 0 56 65 48 847.3 0 48 66 45 847.3 0 45 67 49 847.3 0 49 68 68 847.3 0 68 69 49 847.3 0 49 70 45 847.3 0 45 71 36 847.3 0 36 72 84 847.3 0 84 73 520 847.3 0 520 74 244 847.3 0 244 75 52 847.3 0 52 76 53 847.3 0 53 77 36 847.3 0 36 78 58 847.3 0 58 79 83 847.3 0 83 80 37 847.3 0 37 81 40 847.3 0 40 82 45 847.3 0 45 83 42 847.3 0 42 84 109 847.3 0 109 85 327 847.3 0 327 86 477 847.3 0 477 87 266 847.3 0 266 88 42 847.3 0 42 89 56 847.3 0 56 90 105 847.3 0 105 91 82 847.3 0 82 92 54 847.3 0 54 93 49 847.3 0 49 94 93 847.3 0 93 95 61 847.3 0 61 96 60 847.3 0 60 97 70 847.3 0 70 98 67 847.3 0 67 99 70 847.3 0 70 100 42 847.3 0 42 101 52 847.3 0 52 102 49 847.3 0 49 103 54 847.3 0 54 104 72 847.3 0 72 105 79 847.3 0 79 106 115 847.3 0 115 107 2024 847.3 0 2024 108 1153 847.3 0 1153 109 829 847.3 0 829 110 80 847.3 0 80 111 37 847.3 0 37 112 53 847.3 0 53 113 54 847.3 0 54 114 60 847.3 0 60 115 42 847.3 0 42 116 64 847.3 0 64 117 53 847.3 0 53 118 34 847.3 0 34 119 47 847.3 0 47 120 54 847.3 0 54 121 78 847.3 0 78 122 61 847.3 0 61 123 106 847.3 0 106 124 60 847.3 0 60 125 73 847.3 0 73 126 118 847.3 0 118 127 1159 847.3 0 1159 128 868 847.3 0 868 129 343 847.3 0 343 130 154 847.3 0 154 131 68 847.3 0 68 132 59 847.3 0 59 133 64 847.3 0 64 134 69 847.3 0 69 135 92 847.3 0 92 136 178 847.3 0 178 137 159 847.3 0 159 138 134 847.3 0 134 139 79 847.3 0 79 140 177 847.3 0 177 141 328 847.3 0 328 142 421 847.3 0 421 143 2909 847.3 0 2909 144 1572 847.3 0 1572 145 520 847.3 0 520 146 127 847.3 0 127 147 460 847.3 0 460 148 92 847.3 0 92 149 171 847.3 0 171 150 109 847.3 0 109 151 114 847.3 0 114 152 152 847.3 0 152 153 317 847.3 0 317 154 592 847.3 0 592 155 1729 847.3 0 1729 156 1109 847.3 0 1109 157 82 847.3 0 82 158 115 847.3 0 115 159 114 847.3 0 114 160 90 847.3 0 90 161 90 847.3 0 90 162 112 847.3 0 112 163 43 847.3 0 43 164 56 847.3 0 56 165 60 847.3 0 60 166 110 847.3 0 110 167 122 847.3 0 122 168 87 847.3 0 87 169 296 847.3 0 296 170 195 847.3 0 195 171 153 847.3 0 153 172 63 847.3 0 63 173 101 847.3 0 101 174 187 847.3 0 187 175 211 847.3 0 211 176 208 847.3 0 208 177 84 847.3 0 84 178 90 847.3 0 90 179 107 847.3 0 107 180 130 847.3 0 130 181 156 847.3 0 156 182 327 847.3 0 327 183 265 847.3 0 265 184 390 847.3 0 390 185 415 847.3 0 415 186 3314 847.3 0 3314 187 4131 847.3 0 4131 188 10571 847.3 0 10571 189 125 847.3 0 125 190 55 847.3 0 55 191 65 847.3 0 65 192 60 847.3 0 60 193 46 847.3 0 46 194 76 847.3 0 76 195 118 847.3 0 118 196 60 847.3 0 60 197 50 847.3 0 50 198 48 847.3 0 48 199 61 847.3 0 61 200 77 847.3 0 77 201 57 847.3 0 57 202 56 847.3 0 56 203 88 847.3 0 88 204 186 847.3 0 186 205 113 847.3 0 113 206 80 847.3 0 80 207 67 847.3 0 67 208 90 847.3 0 90 209 121 847.3 0 121 210 114 847.3 0 114 211 79 847.3 0 79 212 66 847.3 0 66 213 89 847.3 0 89 214 70 847.3 0 70 215 96 847.3 0 96 216 84 847.3 0 84 217 334 847.3 0 334 218 894 847.3 0 894 219 618 847.3 0 618 220 79 847.3 0 79 221 66 847.3 0 66 222 93 847.3 0 93 223 82 847.3 0 82 224 52 847.3 0 52 225 70 847.3 0 70 226 69 847.3 0 69 227 101 847.3 0 101 228 122 847.3 0 122 229 50 847.3 0 50 230 83 847.3 0 83 231 101 847.3 0 101 232 98 847.3 0 98 233 85 847.3 0 85 234 77 847.3 0 77 235 101 847.3 0 101 236 91 847.3 0 91 237 99 847.3 0 99 238 66 847.3 0 66 239 83 847.3 0 83 240 95 847.3 0 95 241 133 847.3 0 133 242 156 847.3 0 156 243 211 847.3 0 211 244 137 847.3 0 137 245 176 847.3 0 176 246 211 847.3 0 211 247 128 847.3 0 128 248 111 847.3 0 111 249 79 847.3 0 79 250 445 847.3 0 445 251 400 847.3 0 400 252 198 847.3 0 198 253 169 847.3 0 169 254 239 847.3 0 239 255 271 847.3 0 271 256 88 847.3 0 88 257 99 847.3 0 99 258 106 847.3 0 106 259 102 847.3 0 102 260 92 847.3 0 92 261 170 847.3 0 170 262 276 847.3 0 276 263 213 847.3 0 213 264 154 847.3 0 154 265 65 847.3 0 65 266 92 847.3 0 92 267 92 847.3 0 92 268 82 847.3 0 82 269 119 847.3 0 119 270 154 847.3 0 154 271 219 847.3 0 219 272 191 847.3 0 191 273 196 847.3 0 196 274 81 847.3 0 81 275 90 847.3 0 90 276 120 847.3 0 120 277 115 847.3 0 115 278 277 847.3 0 277 279 296 847.3 0 296 280 701 847.3 0 701 281 499 847.3 0 499 282 552 847.3 0 552 283 150 847.3 0 150 284 118 847.3 0 118 285 194 847.3 0 194 286 199 847.3 0 199 287 97 847.3 0 97 288 108 847.3 0 108 289 172 847.3 0 172 290 190 847.3 0 190 291 184 847.3 0 184 292 118 847.3 0 118 293 128 847.3 0 128 294 161 847.3 0 161 295 240 847.3 0 240 296 374 847.3 0 374 297 649 847.3 0 649 298 709 847.3 0 709 299 668 847.3 0 668 300 373 847.3 0 373 301 114 847.3 0 114 Finished in 10.58 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 142,114 Reads with adapters: 40,365 (28.4%) Reads that were too short: 52 (0.0%) Reads written (passing filters): 40,313 (28.4%) Total basepairs processed: 34,556,903 bp Total written (filtered): 10,992,630 bp (31.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 40365 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 24 0.0 3 0 0 22 2 19 544 0.0 3 0 491 33 14 6 20 39093 0.0 4 37675 1226 145 40 7 21 105 0.0 4 13 88 3 1 22 12 0.0 4 0 0 10 1 1 23 36 0.0 4 0 0 0 32 4 24 1 0.0 4 0 0 0 0 1 25 11 0.0 4 0 0 0 0 11 28 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 1 54 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 1 99 1 0.0 4 0 0 0 0 1 103 2 0.0 4 0 0 0 0 2 106 2 0.0 4 0 0 0 0 2 110 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 126 10 0.0 4 0 0 0 0 10 127 456 0.0 4 0 0 0 0 456 128 2 0.0 4 0 0 0 0 2 134 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 146 2 0.0 4 0 0 0 0 2 148 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 1 153 8 0.0 4 0 0 0 0 8 165 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 185 2 0.0 4 1 0 0 0 1 189 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 7 0.0 4 0 0 0 1 6 234 2 0.0 4 0 0 0 1 1 237 2 0.0 4 0 0 0 0 2 238 10 0.0 4 0 0 0 0 10 257 1 0.0 4 0 0 0 0 1 263 3 0.0 4 0 0 0 0 3 282 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 288 2 0.0 4 0 0 0 0 2 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.29 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 40,313 Reads with adapters: 2,053 (5.1%) Reads written (passing filters): 40,313 (100.0%) Total basepairs processed: 10,992,630 bp Total written (filtered): 10,965,082 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2053 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.8% C: 8.8% G: 82.5% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 137 629.9 0 137 4 11 157.5 0 11 5 28 39.4 0 28 6 4 9.8 0 4 9 4 0.2 0 0 4 10 36 0.0 1 3 33 11 1469 0.0 1 47 1422 12 312 0.0 1 8 304 13 12 0.0 1 0 12 15 4 0.0 1 0 4 16 2 0.0 1 0 2 25 1 0.0 1 0 1 101 1 0.0 1 1 152 7 0.0 1 6 1 153 1 0.0 1 1 208 1 0.0 1 1 210 22 0.0 1 17 5 211 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.