This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 165.36 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 96,203 (2.8%) Reads that were too short: 11,059 (0.3%) Reads written (passing filters): 85,144 (2.5%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 21,179,088 bp (2.0%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 96203 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 17810 847.3 0 17810 7 928 847.3 0 928 8 28774 847.3 0 28774 9 3262 847.3 0 3262 10 17 847.3 0 17 11 11 847.3 0 11 12 15 847.3 0 15 13 18 847.3 0 18 14 16 847.3 0 16 15 15 847.3 0 15 16 14 847.3 0 14 17 33 847.3 0 33 18 22 847.3 0 22 19 17 847.3 0 17 20 15 847.3 0 15 21 18 847.3 0 18 22 33 847.3 0 33 23 15 847.3 0 15 24 21 847.3 0 21 25 18 847.3 0 18 26 19 847.3 0 19 27 21 847.3 0 21 28 22 847.3 0 22 29 13 847.3 0 13 30 27 847.3 0 27 31 21 847.3 0 21 32 14 847.3 0 14 33 28 847.3 0 28 34 35 847.3 0 35 35 16 847.3 0 16 36 23 847.3 0 23 37 19 847.3 0 19 38 16 847.3 0 16 39 15 847.3 0 15 40 31 847.3 0 31 41 17 847.3 0 17 42 23 847.3 0 23 43 23 847.3 0 23 44 26 847.3 0 26 45 30 847.3 0 30 46 40 847.3 0 40 47 85 847.3 0 85 48 35 847.3 0 35 49 215 847.3 0 215 50 256 847.3 0 256 51 22 847.3 0 22 52 22 847.3 0 22 53 35 847.3 0 35 54 13 847.3 0 13 55 29 847.3 0 29 56 20 847.3 0 20 57 24 847.3 0 24 58 44 847.3 0 44 59 60 847.3 0 60 60 59 847.3 0 59 61 44 847.3 0 44 62 49 847.3 0 49 63 19 847.3 0 19 64 24 847.3 0 24 65 16 847.3 0 16 66 301 847.3 0 301 67 277 847.3 0 277 68 160 847.3 0 160 69 13 847.3 0 13 70 21 847.3 0 21 71 14 847.3 0 14 72 12 847.3 0 12 73 30 847.3 0 30 74 20 847.3 0 20 75 19 847.3 0 19 76 27 847.3 0 27 77 18 847.3 0 18 78 18 847.3 0 18 79 20 847.3 0 20 80 28 847.3 0 28 81 18 847.3 0 18 82 65 847.3 0 65 83 63 847.3 0 63 84 53 847.3 0 53 85 38 847.3 0 38 86 55 847.3 0 55 87 24 847.3 0 24 88 22 847.3 0 22 89 211 847.3 0 211 90 70 847.3 0 70 91 57 847.3 0 57 92 65 847.3 0 65 93 118 847.3 0 118 94 66 847.3 0 66 95 45 847.3 0 45 96 27 847.3 0 27 97 54 847.3 0 54 98 87 847.3 0 87 99 76 847.3 0 76 100 60 847.3 0 60 101 60 847.3 0 60 102 59 847.3 0 59 103 122 847.3 0 122 104 167 847.3 0 167 105 62 847.3 0 62 106 78 847.3 0 78 107 111 847.3 0 111 108 94 847.3 0 94 109 84 847.3 0 84 110 145 847.3 0 145 111 305 847.3 0 305 112 356 847.3 0 356 113 326 847.3 0 326 114 196 847.3 0 196 115 398 847.3 0 398 116 724 847.3 0 724 117 9076 847.3 0 9076 118 6607 847.3 0 6607 119 4403 847.3 0 4403 120 56 847.3 0 56 121 47 847.3 0 47 122 32 847.3 0 32 123 21 847.3 0 21 124 26 847.3 0 26 125 37 847.3 0 37 126 126 847.3 0 126 127 90 847.3 0 90 128 34 847.3 0 34 129 44 847.3 0 44 130 40 847.3 0 40 131 77 847.3 0 77 132 86 847.3 0 86 133 70 847.3 0 70 134 67 847.3 0 67 135 37 847.3 0 37 136 53 847.3 0 53 137 51 847.3 0 51 138 82 847.3 0 82 139 45 847.3 0 45 140 56 847.3 0 56 141 122 847.3 0 122 142 66 847.3 0 66 143 32 847.3 0 32 144 63 847.3 0 63 145 79 847.3 0 79 146 109 847.3 0 109 147 109 847.3 0 109 148 69 847.3 0 69 149 62 847.3 0 62 150 82 847.3 0 82 151 951 847.3 0 951 152 841 847.3 0 841 153 501 847.3 0 501 154 141 847.3 0 141 155 43 847.3 0 43 156 40 847.3 0 40 157 46 847.3 0 46 158 52 847.3 0 52 159 34 847.3 0 34 160 39 847.3 0 39 161 47 847.3 0 47 162 156 847.3 0 156 163 86 847.3 0 86 164 54 847.3 0 54 165 52 847.3 0 52 166 44 847.3 0 44 167 36 847.3 0 36 168 33 847.3 0 33 169 61 847.3 0 61 170 40 847.3 0 40 171 47 847.3 0 47 172 54 847.3 0 54 173 51 847.3 0 51 174 26 847.3 0 26 175 23 847.3 0 23 176 34 847.3 0 34 177 29 847.3 0 29 178 36 847.3 0 36 179 92 847.3 0 92 180 68 847.3 0 68 181 124 847.3 0 124 182 150 847.3 0 150 183 55 847.3 0 55 184 48 847.3 0 48 185 118 847.3 0 118 186 33 847.3 0 33 187 35 847.3 0 35 188 35 847.3 0 35 189 39 847.3 0 39 190 51 847.3 0 51 191 47 847.3 0 47 192 109 847.3 0 109 193 33 847.3 0 33 194 26 847.3 0 26 195 33 847.3 0 33 196 31 847.3 0 31 197 34 847.3 0 34 198 45 847.3 0 45 199 44 847.3 0 44 200 41 847.3 0 41 201 39 847.3 0 39 202 33 847.3 0 33 203 29 847.3 0 29 204 48 847.3 0 48 205 40 847.3 0 40 206 50 847.3 0 50 207 51 847.3 0 51 208 40 847.3 0 40 209 38 847.3 0 38 210 40 847.3 0 40 211 51 847.3 0 51 212 41 847.3 0 41 213 76 847.3 0 76 214 67 847.3 0 67 215 52 847.3 0 52 216 77 847.3 0 77 217 112 847.3 0 112 218 312 847.3 0 312 219 345 847.3 0 345 220 411 847.3 0 411 221 203 847.3 0 203 222 35 847.3 0 35 223 44 847.3 0 44 224 60 847.3 0 60 225 72 847.3 0 72 226 108 847.3 0 108 227 82 847.3 0 82 228 77 847.3 0 77 229 127 847.3 0 127 230 74 847.3 0 74 231 256 847.3 0 256 232 310 847.3 0 310 233 609 847.3 0 609 234 322 847.3 0 322 235 137 847.3 0 137 236 111 847.3 0 111 237 115 847.3 0 115 238 99 847.3 0 99 239 81 847.3 0 81 240 49 847.3 0 49 241 36 847.3 0 36 242 57 847.3 0 57 243 71 847.3 0 71 244 81 847.3 0 81 245 148 847.3 0 148 246 197 847.3 0 197 247 272 847.3 0 272 248 181 847.3 0 181 249 97 847.3 0 97 250 88 847.3 0 88 251 121 847.3 0 121 252 185 847.3 0 185 253 109 847.3 0 109 254 225 847.3 0 225 255 195 847.3 0 195 256 129 847.3 0 129 257 52 847.3 0 52 258 58 847.3 0 58 259 75 847.3 0 75 260 43 847.3 0 43 261 50 847.3 0 50 262 48 847.3 0 48 263 60 847.3 0 60 264 64 847.3 0 64 265 80 847.3 0 80 266 90 847.3 0 90 267 84 847.3 0 84 268 78 847.3 0 78 269 56 847.3 0 56 270 44 847.3 0 44 271 94 847.3 0 94 272 110 847.3 0 110 273 67 847.3 0 67 274 70 847.3 0 70 275 49 847.3 0 49 276 50 847.3 0 50 277 59 847.3 0 59 278 203 847.3 0 203 279 172 847.3 0 172 280 136 847.3 0 136 281 70 847.3 0 70 282 93 847.3 0 93 283 148 847.3 0 148 284 130 847.3 0 130 285 83 847.3 0 83 286 112 847.3 0 112 287 90 847.3 0 90 288 91 847.3 0 91 289 58 847.3 0 58 290 73 847.3 0 73 291 121 847.3 0 121 292 126 847.3 0 126 293 179 847.3 0 179 294 90 847.3 0 90 295 90 847.3 0 90 296 73 847.3 0 73 297 76 847.3 0 76 298 91 847.3 0 91 299 115 847.3 0 115 300 115 847.3 0 115 301 67 847.3 0 67 Finished in 6.34 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 85,144 Reads with adapters: 21,148 (24.8%) Reads that were too short: 45 (0.1%) Reads written (passing filters): 21,103 (24.8%) Total basepairs processed: 21,179,088 bp Total written (filtered): 5,748,581 bp (27.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 21148 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 2 0.0 3 0 0 2 19 48 0.0 3 0 34 10 2 2 20 3633 0.0 4 3149 426 42 10 6 21 17094 0.0 4 16380 590 99 18 7 22 32 0.0 4 8 23 1 23 16 0.0 4 0 0 2 13 1 24 24 0.0 4 1 0 0 20 3 27 1 0.0 4 1 33 1 0.0 4 1 34 1 0.0 4 1 42 1 0.0 4 1 44 1 0.0 4 0 0 0 1 50 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 1 89 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 100 1 0.0 4 1 105 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 3 0.0 4 0 0 0 0 3 128 236 0.0 4 0 0 0 0 236 129 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 133 3 0.0 4 0 0 0 0 3 134 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 154 9 0.0 4 0 0 0 0 9 157 1 0.0 4 0 0 0 0 1 158 2 0.0 4 0 0 0 0 2 161 1 0.0 4 0 0 0 0 1 182 2 0.0 4 0 0 0 0 2 183 2 0.0 4 0 0 0 0 2 202 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 230 3 0.0 4 0 1 0 0 2 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.22 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 21,103 Reads with adapters: 2,068 (9.8%) Reads written (passing filters): 21,103 (100.0%) Total basepairs processed: 5,748,581 bp Total written (filtered): 5,721,558 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2068 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.9% C: 8.0% G: 85.8% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 51 329.7 0 51 4 12 82.4 0 12 8 5 0.3 0 5 9 153 0.1 0 10 143 10 455 0.0 1 34 421 11 1254 0.0 1 32 1222 12 107 0.0 1 1 106 13 3 0.0 1 0 3 123 1 0.0 1 1 152 2 0.0 1 2 208 1 0.0 1 0 1 210 23 0.0 1 17 6 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.