This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 169.38 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 403,257 (11.6%) Reads that were too short: 19,113 (0.6%) Reads written (passing filters): 384,144 (11.1%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 80,252,448 bp (7.7%) === Adapter 1 === Sequence: AAGGTC; Type: regular 5'; Length: 6; Trimmed: 403257 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 84 847.3 0 84 7 98 847.3 0 98 8 81 847.3 0 81 9 167 847.3 0 167 10 72 847.3 0 72 11 60 847.3 0 60 12 142 847.3 0 142 13 364 847.3 0 364 14 65 847.3 0 65 15 81 847.3 0 81 16 95 847.3 0 95 17 86 847.3 0 86 18 89 847.3 0 89 19 83 847.3 0 83 20 93 847.3 0 93 21 103 847.3 0 103 22 77 847.3 0 77 23 81 847.3 0 81 24 78 847.3 0 78 25 69 847.3 0 69 26 62 847.3 0 62 27 70 847.3 0 70 28 51 847.3 0 51 29 66 847.3 0 66 30 65 847.3 0 65 31 73 847.3 0 73 32 86 847.3 0 86 33 61 847.3 0 61 34 74 847.3 0 74 35 131 847.3 0 131 36 92 847.3 0 92 37 72 847.3 0 72 38 64 847.3 0 64 39 65 847.3 0 65 40 93 847.3 0 93 41 76 847.3 0 76 42 94 847.3 0 94 43 107 847.3 0 107 44 87 847.3 0 87 45 107 847.3 0 107 46 94 847.3 0 94 47 76 847.3 0 76 48 70 847.3 0 70 49 97 847.3 0 97 50 90 847.3 0 90 51 124 847.3 0 124 52 108 847.3 0 108 53 109 847.3 0 109 54 149 847.3 0 149 55 131 847.3 0 131 56 159 847.3 0 159 57 137 847.3 0 137 58 80 847.3 0 80 59 100 847.3 0 100 60 146 847.3 0 146 61 118 847.3 0 118 62 291 847.3 0 291 63 128 847.3 0 128 64 149 847.3 0 149 65 109 847.3 0 109 66 265 847.3 0 265 67 166 847.3 0 166 68 244 847.3 0 244 69 376 847.3 0 376 70 578 847.3 0 578 71 670 847.3 0 670 72 1362 847.3 0 1362 73 2609 847.3 0 2609 74 740 847.3 0 740 75 882 847.3 0 882 76 1488 847.3 0 1488 77 901 847.3 0 901 78 816 847.3 0 816 79 1885 847.3 0 1885 80 1701 847.3 0 1701 81 1688 847.3 0 1688 82 1844 847.3 0 1844 83 2809 847.3 0 2809 84 4009 847.3 0 4009 85 6982 847.3 0 6982 86 137525 847.3 0 137525 87 107871 847.3 0 107871 88 61536 847.3 0 61536 89 280 847.3 0 280 90 57 847.3 0 57 91 57 847.3 0 57 92 71 847.3 0 71 93 58 847.3 0 58 94 51 847.3 0 51 95 65 847.3 0 65 96 89 847.3 0 89 97 77 847.3 0 77 98 60 847.3 0 60 99 76 847.3 0 76 100 72 847.3 0 72 101 76 847.3 0 76 102 76 847.3 0 76 103 333 847.3 0 333 104 230 847.3 0 230 105 148 847.3 0 148 106 51 847.3 0 51 107 54 847.3 0 54 108 83 847.3 0 83 109 61 847.3 0 61 110 64 847.3 0 64 111 98 847.3 0 98 112 62 847.3 0 62 113 61 847.3 0 61 114 69 847.3 0 69 115 96 847.3 0 96 116 188 847.3 0 188 117 71 847.3 0 71 118 85 847.3 0 85 119 66 847.3 0 66 120 87 847.3 0 87 121 106 847.3 0 106 122 118 847.3 0 118 123 496 847.3 0 496 124 1618 847.3 0 1618 125 759 847.3 0 759 126 792 847.3 0 792 127 240 847.3 0 240 128 141 847.3 0 141 129 196 847.3 0 196 130 180 847.3 0 180 131 189 847.3 0 189 132 87 847.3 0 87 133 97 847.3 0 97 134 182 847.3 0 182 135 145 847.3 0 145 136 152 847.3 0 152 137 144 847.3 0 144 138 139 847.3 0 139 139 146 847.3 0 146 140 174 847.3 0 174 141 345 847.3 0 345 142 324 847.3 0 324 143 451 847.3 0 451 144 5516 847.3 0 5516 145 5399 847.3 0 5399 146 2462 847.3 0 2462 147 185 847.3 0 185 148 65 847.3 0 65 149 110 847.3 0 110 150 105 847.3 0 105 151 70 847.3 0 70 152 79 847.3 0 79 153 96 847.3 0 96 154 83 847.3 0 83 155 102 847.3 0 102 156 104 847.3 0 104 157 87 847.3 0 87 158 100 847.3 0 100 159 75 847.3 0 75 160 82 847.3 0 82 161 77 847.3 0 77 162 77 847.3 0 77 163 72 847.3 0 72 164 80 847.3 0 80 165 103 847.3 0 103 166 230 847.3 0 230 167 415 847.3 0 415 168 695 847.3 0 695 169 157 847.3 0 157 170 157 847.3 0 157 171 166 847.3 0 166 172 139 847.3 0 139 173 88 847.3 0 88 174 115 847.3 0 115 175 103 847.3 0 103 176 117 847.3 0 117 177 96 847.3 0 96 178 112 847.3 0 112 179 89 847.3 0 89 180 112 847.3 0 112 181 134 847.3 0 134 182 168 847.3 0 168 183 195 847.3 0 195 184 280 847.3 0 280 185 4439 847.3 0 4439 186 2871 847.3 0 2871 187 1277 847.3 0 1277 188 128 847.3 0 128 189 73 847.3 0 73 190 68 847.3 0 68 191 85 847.3 0 85 192 122 847.3 0 122 193 137 847.3 0 137 194 112 847.3 0 112 195 148 847.3 0 148 196 108 847.3 0 108 197 83 847.3 0 83 198 81 847.3 0 81 199 118 847.3 0 118 200 114 847.3 0 114 201 114 847.3 0 114 202 110 847.3 0 110 203 150 847.3 0 150 204 161 847.3 0 161 205 217 847.3 0 217 206 2800 847.3 0 2800 207 2334 847.3 0 2334 208 993 847.3 0 993 209 108 847.3 0 108 210 394 847.3 0 394 211 773 847.3 0 773 212 757 847.3 0 757 213 506 847.3 0 506 214 90 847.3 0 90 215 67 847.3 0 67 216 71 847.3 0 71 217 64 847.3 0 64 218 57 847.3 0 57 219 75 847.3 0 75 220 103 847.3 0 103 221 85 847.3 0 85 222 93 847.3 0 93 223 72 847.3 0 72 224 78 847.3 0 78 225 92 847.3 0 92 226 205 847.3 0 205 227 217 847.3 0 217 228 199 847.3 0 199 229 101 847.3 0 101 230 50 847.3 0 50 231 97 847.3 0 97 232 76 847.3 0 76 233 76 847.3 0 76 234 80 847.3 0 80 235 69 847.3 0 69 236 67 847.3 0 67 237 79 847.3 0 79 238 78 847.3 0 78 239 74 847.3 0 74 240 80 847.3 0 80 241 150 847.3 0 150 242 139 847.3 0 139 243 87 847.3 0 87 244 73 847.3 0 73 245 94 847.3 0 94 246 135 847.3 0 135 247 116 847.3 0 116 248 83 847.3 0 83 249 72 847.3 0 72 250 87 847.3 0 87 251 61 847.3 0 61 252 72 847.3 0 72 253 54 847.3 0 54 254 73 847.3 0 73 255 238 847.3 0 238 256 141 847.3 0 141 257 163 847.3 0 163 258 102 847.3 0 102 259 427 847.3 0 427 260 459 847.3 0 459 261 339 847.3 0 339 262 141 847.3 0 141 263 85 847.3 0 85 264 70 847.3 0 70 265 54 847.3 0 54 266 102 847.3 0 102 267 228 847.3 0 228 268 185 847.3 0 185 269 116 847.3 0 116 270 83 847.3 0 83 271 54 847.3 0 54 272 85 847.3 0 85 273 90 847.3 0 90 274 82 847.3 0 82 275 137 847.3 0 137 276 132 847.3 0 132 277 84 847.3 0 84 278 56 847.3 0 56 279 105 847.3 0 105 280 111 847.3 0 111 281 157 847.3 0 157 282 149 847.3 0 149 283 77 847.3 0 77 284 58 847.3 0 58 285 49 847.3 0 49 286 53 847.3 0 53 287 70 847.3 0 70 288 93 847.3 0 93 289 62 847.3 0 62 290 68 847.3 0 68 291 64 847.3 0 64 292 73 847.3 0 73 293 74 847.3 0 74 294 136 847.3 0 136 295 149 847.3 0 149 296 168 847.3 0 168 297 92 847.3 0 92 298 70 847.3 0 70 299 73 847.3 0 73 300 155 847.3 0 155 301 260 847.3 0 260 Finished in 24.65 s (64 us/read; 0.94 M reads/minute). === Summary === Total reads processed: 384,144 Reads with adapters: 242 (0.1%) Reads that were too short: 119 (0.0%) Reads written (passing filters): 123 (0.0%) Total basepairs processed: 80,252,448 bp Total written (filtered): 23,129 bp (0.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 242 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 20 31 0.0 4 29 2 22 3 0.0 4 2 1 23 6 0.0 4 5 0 0 0 1 29 2 0.0 4 0 1 0 0 1 34 1 0.0 4 0 0 0 0 1 38 1 0.0 4 1 48 14 0.0 4 0 0 0 0 14 49 4 0.0 4 0 0 0 0 4 52 2 0.0 4 0 0 0 0 2 58 2 0.0 4 0 1 0 0 1 65 1 0.0 4 0 0 0 0 1 70 2 0.0 4 1 0 0 0 1 74 51 0.0 4 0 0 0 0 51 87 2 0.0 4 0 0 0 0 2 90 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 1 95 8 0.0 4 0 0 0 1 7 97 2 0.0 4 0 0 0 0 2 116 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 128 4 0.0 4 0 0 0 0 4 129 3 0.0 4 0 0 0 1 2 131 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 146 5 0.0 4 0 0 0 1 4 148 1 0.0 4 0 0 0 0 1 149 25 0.0 4 0 0 0 0 25 150 9 0.0 4 0 0 0 0 9 152 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 154 4 0.0 4 0 1 0 0 3 155 12 0.0 4 0 0 0 0 12 156 12 0.0 4 0 0 0 0 12 163 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 178 3 0.0 4 0 0 0 0 3 179 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 209 3 0.0 4 0 0 0 0 3 211 2 0.0 4 0 0 0 0 2 213 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 0 2 216 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (81 us/read; 0.74 M reads/minute). === Summary === Total reads processed: 123 Reads with adapters: 7 (5.7%) Reads written (passing filters): 123 (100.0%) Total basepairs processed: 23,129 bp Total written (filtered): 22,950 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 14.3% C: 14.3% G: 71.4% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 1.9 0 1 9 1 0.0 0 1 11 3 0.0 1 0 3 13 1 0.0 1 0 1 121 1 0.0 1 1