This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 165.32 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 198,613 (5.7%) Reads that were too short: 39,686 (1.1%) Reads written (passing filters): 158,927 (4.6%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 38,351,048 bp (3.7%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 198613 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4704 847.3 0 4704 7 3772 847.3 0 3772 8 41906 847.3 0 41906 9 33894 847.3 0 33894 10 213 847.3 0 213 11 96 847.3 0 96 12 109 847.3 0 109 13 96 847.3 0 96 14 102 847.3 0 102 15 164 847.3 0 164 16 127 847.3 0 127 17 123 847.3 0 123 18 111 847.3 0 111 19 144 847.3 0 144 20 89 847.3 0 89 21 125 847.3 0 125 22 110 847.3 0 110 23 128 847.3 0 128 24 128 847.3 0 128 25 126 847.3 0 126 26 129 847.3 0 129 27 157 847.3 0 157 28 123 847.3 0 123 29 121 847.3 0 121 30 146 847.3 0 146 31 129 847.3 0 129 32 172 847.3 0 172 33 203 847.3 0 203 34 120 847.3 0 120 35 206 847.3 0 206 36 190 847.3 0 190 37 2220 847.3 0 2220 38 1688 847.3 0 1688 39 1714 847.3 0 1714 40 394 847.3 0 394 41 163 847.3 0 163 42 124 847.3 0 124 43 149 847.3 0 149 44 150 847.3 0 150 45 156 847.3 0 156 46 133 847.3 0 133 47 137 847.3 0 137 48 131 847.3 0 131 49 124 847.3 0 124 50 93 847.3 0 93 51 145 847.3 0 145 52 130 847.3 0 130 53 101 847.3 0 101 54 116 847.3 0 116 55 107 847.3 0 107 56 125 847.3 0 125 57 135 847.3 0 135 58 114 847.3 0 114 59 114 847.3 0 114 60 121 847.3 0 121 61 98 847.3 0 98 62 108 847.3 0 108 63 124 847.3 0 124 64 98 847.3 0 98 65 108 847.3 0 108 66 119 847.3 0 119 67 115 847.3 0 115 68 124 847.3 0 124 69 128 847.3 0 128 70 133 847.3 0 133 71 133 847.3 0 133 72 144 847.3 0 144 73 114 847.3 0 114 74 146 847.3 0 146 75 175 847.3 0 175 76 188 847.3 0 188 77 310 847.3 0 310 78 303 847.3 0 303 79 190 847.3 0 190 80 237 847.3 0 237 81 131 847.3 0 131 82 148 847.3 0 148 83 146 847.3 0 146 84 113 847.3 0 113 85 120 847.3 0 120 86 119 847.3 0 119 87 138 847.3 0 138 88 246 847.3 0 246 89 189 847.3 0 189 90 221 847.3 0 221 91 457 847.3 0 457 92 1079 847.3 0 1079 93 1516 847.3 0 1516 94 278 847.3 0 278 95 365 847.3 0 365 96 136 847.3 0 136 97 125 847.3 0 125 98 122 847.3 0 122 99 118 847.3 0 118 100 124 847.3 0 124 101 90 847.3 0 90 102 102 847.3 0 102 103 113 847.3 0 113 104 200 847.3 0 200 105 277 847.3 0 277 106 263 847.3 0 263 107 148 847.3 0 148 108 141 847.3 0 141 109 149 847.3 0 149 110 161 847.3 0 161 111 122 847.3 0 122 112 321 847.3 0 321 113 330 847.3 0 330 114 261 847.3 0 261 115 265 847.3 0 265 116 251 847.3 0 251 117 159 847.3 0 159 118 189 847.3 0 189 119 156 847.3 0 156 120 140 847.3 0 140 121 149 847.3 0 149 122 193 847.3 0 193 123 151 847.3 0 151 124 201 847.3 0 201 125 156 847.3 0 156 126 181 847.3 0 181 127 211 847.3 0 211 128 440 847.3 0 440 129 2667 847.3 0 2667 130 956 847.3 0 956 131 920 847.3 0 920 132 222 847.3 0 222 133 235 847.3 0 235 134 223 847.3 0 223 135 247 847.3 0 247 136 285 847.3 0 285 137 330 847.3 0 330 138 312 847.3 0 312 139 457 847.3 0 457 140 537 847.3 0 537 141 1294 847.3 0 1294 142 21487 847.3 0 21487 143 6646 847.3 0 6646 144 3913 847.3 0 3913 145 101 847.3 0 101 146 139 847.3 0 139 147 120 847.3 0 120 148 143 847.3 0 143 149 334 847.3 0 334 150 302 847.3 0 302 151 199 847.3 0 199 152 112 847.3 0 112 153 142 847.3 0 142 154 142 847.3 0 142 155 117 847.3 0 117 156 132 847.3 0 132 157 135 847.3 0 135 158 117 847.3 0 117 159 120 847.3 0 120 160 103 847.3 0 103 161 91 847.3 0 91 162 125 847.3 0 125 163 144 847.3 0 144 164 114 847.3 0 114 165 103 847.3 0 103 166 138 847.3 0 138 167 114 847.3 0 114 168 95 847.3 0 95 169 137 847.3 0 137 170 169 847.3 0 169 171 98 847.3 0 98 172 85 847.3 0 85 173 134 847.3 0 134 174 109 847.3 0 109 175 103 847.3 0 103 176 95 847.3 0 95 177 119 847.3 0 119 178 140 847.3 0 140 179 130 847.3 0 130 180 110 847.3 0 110 181 80 847.3 0 80 182 132 847.3 0 132 183 114 847.3 0 114 184 75 847.3 0 75 185 101 847.3 0 101 186 135 847.3 0 135 187 95 847.3 0 95 188 125 847.3 0 125 189 110 847.3 0 110 190 109 847.3 0 109 191 124 847.3 0 124 192 114 847.3 0 114 193 91 847.3 0 91 194 120 847.3 0 120 195 96 847.3 0 96 196 117 847.3 0 117 197 151 847.3 0 151 198 95 847.3 0 95 199 94 847.3 0 94 200 119 847.3 0 119 201 135 847.3 0 135 202 147 847.3 0 147 203 132 847.3 0 132 204 176 847.3 0 176 205 165 847.3 0 165 206 119 847.3 0 119 207 151 847.3 0 151 208 154 847.3 0 154 209 553 847.3 0 553 210 436 847.3 0 436 211 252 847.3 0 252 212 266 847.3 0 266 213 208 847.3 0 208 214 1631 847.3 0 1631 215 1376 847.3 0 1376 216 1981 847.3 0 1981 217 224 847.3 0 224 218 149 847.3 0 149 219 133 847.3 0 133 220 132 847.3 0 132 221 214 847.3 0 214 222 153 847.3 0 153 223 116 847.3 0 116 224 214 847.3 0 214 225 174 847.3 0 174 226 249 847.3 0 249 227 277 847.3 0 277 228 248 847.3 0 248 229 657 847.3 0 657 230 503 847.3 0 503 231 474 847.3 0 474 232 331 847.3 0 331 233 503 847.3 0 503 234 5293 847.3 0 5293 235 5916 847.3 0 5916 236 3983 847.3 0 3983 237 973 847.3 0 973 238 104 847.3 0 104 239 105 847.3 0 105 240 88 847.3 0 88 241 97 847.3 0 97 242 78 847.3 0 78 243 111 847.3 0 111 244 104 847.3 0 104 245 88 847.3 0 88 246 121 847.3 0 121 247 89 847.3 0 89 248 76 847.3 0 76 249 90 847.3 0 90 250 102 847.3 0 102 251 105 847.3 0 105 252 108 847.3 0 108 253 193 847.3 0 193 254 312 847.3 0 312 255 267 847.3 0 267 256 166 847.3 0 166 257 93 847.3 0 93 258 103 847.3 0 103 259 110 847.3 0 110 260 116 847.3 0 116 261 139 847.3 0 139 262 503 847.3 0 503 263 417 847.3 0 417 264 520 847.3 0 520 265 323 847.3 0 323 266 149 847.3 0 149 267 81 847.3 0 81 268 88 847.3 0 88 269 80 847.3 0 80 270 120 847.3 0 120 271 141 847.3 0 141 272 131 847.3 0 131 273 79 847.3 0 79 274 99 847.3 0 99 275 121 847.3 0 121 276 139 847.3 0 139 277 188 847.3 0 188 278 106 847.3 0 106 279 76 847.3 0 76 280 86 847.3 0 86 281 114 847.3 0 114 282 97 847.3 0 97 283 102 847.3 0 102 284 145 847.3 0 145 285 120 847.3 0 120 286 89 847.3 0 89 287 75 847.3 0 75 288 87 847.3 0 87 289 63 847.3 0 63 290 62 847.3 0 62 291 246 847.3 0 246 292 236 847.3 0 236 293 208 847.3 0 208 294 113 847.3 0 113 295 272 847.3 0 272 296 481 847.3 0 481 297 845 847.3 0 845 298 555 847.3 0 555 299 329 847.3 0 329 300 93 847.3 0 93 301 279 847.3 0 279 Finished in 11.51 s (72 us/read; 0.83 M reads/minute). === Summary === Total reads processed: 158,927 Reads with adapters: 37,994 (23.9%) Reads that were too short: 40 (0.0%) Reads written (passing filters): 37,954 (23.9%) Total basepairs processed: 38,351,048 bp Total written (filtered): 10,311,334 bp (26.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 37994 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 1 3 18 15 0.0 3 0 0 8 3 4 19 348 0.0 3 0 295 16 28 9 20 34911 0.0 4 32943 1611 256 75 26 21 2192 0.0 4 2011 151 20 7 3 22 24 0.0 4 4 11 7 0 2 23 286 0.0 4 154 8 3 81 40 24 10 0.0 4 0 1 0 5 4 26 3 0.0 4 0 0 0 2 1 27 1 0.0 4 1 28 1 0.0 4 0 0 0 0 1 42 56 0.0 4 0 0 0 0 56 43 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 69 2 0.0 4 0 0 0 0 2 74 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 97 57 0.0 4 0 0 0 0 57 100 3 0.0 4 0 0 0 0 3 110 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 127 2 0.0 4 0 0 0 0 2 128 36 0.0 4 0 0 0 0 36 151 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 192 1 0.0 4 1 198 3 0.0 4 0 0 0 1 2 206 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 1 211 2 0.0 4 0 0 0 0 2 214 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 258 3 0.0 4 0 0 1 0 2 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.40 s (63 us/read; 0.95 M reads/minute). === Summary === Total reads processed: 37,954 Reads with adapters: 11,955 (31.5%) Reads written (passing filters): 37,954 (100.0%) Total basepairs processed: 10,311,334 bp Total written (filtered): 10,189,728 bp (98.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 11955 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.1% C: 5.6% G: 91.8% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 15 593.0 0 15 4 8 148.3 0 8 5 2 37.1 0 2 7 3 2.3 0 3 8 6 0.6 0 6 9 7209 0.1 0 507 6702 10 3387 0.0 1 226 3161 11 1231 0.0 1 30 1201 12 33 0.0 1 0 33 13 11 0.0 1 0 11 14 1 0.0 1 0 1 15 1 0.0 1 0 1 22 1 0.0 1 1 70 1 0.0 1 1 135 1 0.0 1 1 150 17 0.0 1 16 1 151 1 0.0 1 1 152 2 0.0 1 2 177 1 0.0 1 1 208 16 0.0 1 12 4 209 2 0.0 1 2 224 4 0.0 1 4 268 1 0.0 1 1 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.