This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 166.20 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 29,854 (0.9%) Reads that were too short: 14,397 (0.4%) Reads written (passing filters): 15,457 (0.4%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 2,781,441 bp (0.3%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 29854 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 486 847.3 0 486 7 69 847.3 0 69 8 207 847.3 0 207 9 47 847.3 0 47 10 17 847.3 0 17 11 11 847.3 0 11 12 14 847.3 0 14 13 15 847.3 0 15 14 12 847.3 0 12 15 265 847.3 0 265 16 126 847.3 0 126 17 10 847.3 0 10 18 12 847.3 0 12 19 12 847.3 0 12 20 13 847.3 0 13 21 23 847.3 0 23 22 19 847.3 0 19 23 27 847.3 0 27 24 5 847.3 0 5 25 14 847.3 0 14 26 20 847.3 0 20 27 12 847.3 0 12 28 14 847.3 0 14 29 13 847.3 0 13 30 21 847.3 0 21 31 17 847.3 0 17 32 33 847.3 0 33 33 37 847.3 0 37 34 13 847.3 0 13 35 20 847.3 0 20 36 17 847.3 0 17 37 16 847.3 0 16 38 30 847.3 0 30 39 21 847.3 0 21 40 45 847.3 0 45 41 164 847.3 0 164 42 105 847.3 0 105 43 19 847.3 0 19 44 13 847.3 0 13 45 16 847.3 0 16 46 12 847.3 0 12 47 29 847.3 0 29 48 11 847.3 0 11 49 33 847.3 0 33 50 14 847.3 0 14 51 31 847.3 0 31 52 19 847.3 0 19 53 21 847.3 0 21 54 13 847.3 0 13 55 17 847.3 0 17 56 15 847.3 0 15 57 34 847.3 0 34 58 24 847.3 0 24 59 17 847.3 0 17 60 13 847.3 0 13 61 19 847.3 0 19 62 16 847.3 0 16 63 21 847.3 0 21 64 20 847.3 0 20 65 18 847.3 0 18 66 27 847.3 0 27 67 23 847.3 0 23 68 18 847.3 0 18 69 75 847.3 0 75 70 44 847.3 0 44 71 342 847.3 0 342 72 27 847.3 0 27 73 15 847.3 0 15 74 22 847.3 0 22 75 25 847.3 0 25 76 33 847.3 0 33 77 25 847.3 0 25 78 23 847.3 0 23 79 25 847.3 0 25 80 28 847.3 0 28 81 53 847.3 0 53 82 62 847.3 0 62 83 34 847.3 0 34 84 36 847.3 0 36 85 48 847.3 0 48 86 55 847.3 0 55 87 142 847.3 0 142 88 50 847.3 0 50 89 71 847.3 0 71 90 91 847.3 0 91 91 93 847.3 0 93 92 77 847.3 0 77 93 53 847.3 0 53 94 52 847.3 0 52 95 41 847.3 0 41 96 72 847.3 0 72 97 75 847.3 0 75 98 57 847.3 0 57 99 56 847.3 0 56 100 367 847.3 0 367 101 485 847.3 0 485 102 781 847.3 0 781 103 147 847.3 0 147 104 64 847.3 0 64 105 44 847.3 0 44 106 31 847.3 0 31 107 29 847.3 0 29 108 39 847.3 0 39 109 31 847.3 0 31 110 36 847.3 0 36 111 40 847.3 0 40 112 32 847.3 0 32 113 32 847.3 0 32 114 30 847.3 0 30 115 32 847.3 0 32 116 37 847.3 0 37 117 49 847.3 0 49 118 105 847.3 0 105 119 40 847.3 0 40 120 21 847.3 0 21 121 32 847.3 0 32 122 64 847.3 0 64 123 88 847.3 0 88 124 65 847.3 0 65 125 69 847.3 0 69 126 58 847.3 0 58 127 29 847.3 0 29 128 40 847.3 0 40 129 46 847.3 0 46 130 46 847.3 0 46 131 92 847.3 0 92 132 291 847.3 0 291 133 275 847.3 0 275 134 62 847.3 0 62 135 50 847.3 0 50 136 64 847.3 0 64 137 70 847.3 0 70 138 90 847.3 0 90 139 134 847.3 0 134 140 72 847.3 0 72 141 220 847.3 0 220 142 95 847.3 0 95 143 145 847.3 0 145 144 140 847.3 0 140 145 175 847.3 0 175 146 48 847.3 0 48 147 48 847.3 0 48 148 70 847.3 0 70 149 79 847.3 0 79 150 76 847.3 0 76 151 74 847.3 0 74 152 86 847.3 0 86 153 77 847.3 0 77 154 62 847.3 0 62 155 57 847.3 0 57 156 80 847.3 0 80 157 92 847.3 0 92 158 183 847.3 0 183 159 153 847.3 0 153 160 366 847.3 0 366 161 160 847.3 0 160 162 296 847.3 0 296 163 79 847.3 0 79 164 64 847.3 0 64 165 43 847.3 0 43 166 41 847.3 0 41 167 82 847.3 0 82 168 77 847.3 0 77 169 82 847.3 0 82 170 100 847.3 0 100 171 74 847.3 0 74 172 64 847.3 0 64 173 69 847.3 0 69 174 56 847.3 0 56 175 66 847.3 0 66 176 341 847.3 0 341 177 301 847.3 0 301 178 628 847.3 0 628 179 86 847.3 0 86 180 79 847.3 0 79 181 70 847.3 0 70 182 57 847.3 0 57 183 83 847.3 0 83 184 94 847.3 0 94 185 103 847.3 0 103 186 74 847.3 0 74 187 99 847.3 0 99 188 130 847.3 0 130 189 142 847.3 0 142 190 100 847.3 0 100 191 101 847.3 0 101 192 35 847.3 0 35 193 50 847.3 0 50 194 45 847.3 0 45 195 73 847.3 0 73 196 60 847.3 0 60 197 92 847.3 0 92 198 102 847.3 0 102 199 76 847.3 0 76 200 70 847.3 0 70 201 61 847.3 0 61 202 83 847.3 0 83 203 93 847.3 0 93 204 82 847.3 0 82 205 72 847.3 0 72 206 65 847.3 0 65 207 69 847.3 0 69 208 66 847.3 0 66 209 70 847.3 0 70 210 128 847.3 0 128 211 196 847.3 0 196 212 174 847.3 0 174 213 307 847.3 0 307 214 89 847.3 0 89 215 91 847.3 0 91 216 73 847.3 0 73 217 88 847.3 0 88 218 73 847.3 0 73 219 80 847.3 0 80 220 71 847.3 0 71 221 74 847.3 0 74 222 102 847.3 0 102 223 159 847.3 0 159 224 212 847.3 0 212 225 189 847.3 0 189 226 122 847.3 0 122 227 87 847.3 0 87 228 84 847.3 0 84 229 72 847.3 0 72 230 90 847.3 0 90 231 74 847.3 0 74 232 64 847.3 0 64 233 78 847.3 0 78 234 82 847.3 0 82 235 104 847.3 0 104 236 101 847.3 0 101 237 117 847.3 0 117 238 228 847.3 0 228 239 245 847.3 0 245 240 411 847.3 0 411 241 111 847.3 0 111 242 125 847.3 0 125 243 191 847.3 0 191 244 136 847.3 0 136 245 139 847.3 0 139 246 135 847.3 0 135 247 153 847.3 0 153 248 134 847.3 0 134 249 136 847.3 0 136 250 112 847.3 0 112 251 135 847.3 0 135 252 147 847.3 0 147 253 130 847.3 0 130 254 115 847.3 0 115 255 100 847.3 0 100 256 130 847.3 0 130 257 159 847.3 0 159 258 148 847.3 0 148 259 101 847.3 0 101 260 124 847.3 0 124 261 123 847.3 0 123 262 220 847.3 0 220 263 284 847.3 0 284 264 421 847.3 0 421 265 309 847.3 0 309 266 352 847.3 0 352 267 130 847.3 0 130 268 78 847.3 0 78 269 87 847.3 0 87 270 140 847.3 0 140 271 150 847.3 0 150 272 153 847.3 0 153 273 183 847.3 0 183 274 195 847.3 0 195 275 170 847.3 0 170 276 212 847.3 0 212 277 157 847.3 0 157 278 179 847.3 0 179 279 123 847.3 0 123 280 91 847.3 0 91 281 118 847.3 0 118 282 147 847.3 0 147 283 143 847.3 0 143 284 165 847.3 0 165 285 139 847.3 0 139 286 157 847.3 0 157 287 171 847.3 0 171 288 167 847.3 0 167 289 126 847.3 0 126 290 119 847.3 0 119 291 158 847.3 0 158 292 196 847.3 0 196 293 142 847.3 0 142 294 120 847.3 0 120 295 105 847.3 0 105 296 90 847.3 0 90 297 110 847.3 0 110 298 163 847.3 0 163 299 202 847.3 0 202 300 222 847.3 0 222 301 354 847.3 0 354 Finished in 0.87 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 15,457 Reads with adapters: 569 (3.7%) Reads that were too short: 25 (0.2%) Reads written (passing filters): 544 (3.5%) Total basepairs processed: 2,781,441 bp Total written (filtered): 139,753 bp (5.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 569 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 1 0.0 3 0 1 17 23 0.0 3 22 1 19 9 0.0 3 0 8 1 20 437 0.0 3 429 7 1 21 1 0.0 3 0 1 22 12 0.0 3 0 0 0 12 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 34 4 0.0 3 0 0 0 4 42 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 0 3 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 71 18 0.0 3 0 0 0 18 72 10 0.0 3 0 0 0 10 73 1 0.0 3 0 0 0 1 74 4 0.0 3 0 0 1 3 75 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 187 3 0.0 3 0 0 0 3 188 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 544 Reads with adapters: 20 (3.7%) Reads written (passing filters): 544 (100.0%) Total basepairs processed: 139,753 bp Total written (filtered): 139,693 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 20 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 90.0% C: 5.0% G: 5.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 20 8.5 0 20 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.