This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 160.59 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 87,335 (2.5%) Reads that were too short: 46,769 (1.3%) Reads written (passing filters): 40,566 (1.2%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 10,078,238 bp (1.0%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 87335 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 356 847.3 0 356 7 500 847.3 0 500 8 11899 847.3 0 11899 9 10672 847.3 0 10672 10 99 847.3 0 99 11 55 847.3 0 55 12 72 847.3 0 72 13 241 847.3 0 241 14 69 847.3 0 69 15 54 847.3 0 54 16 57 847.3 0 57 17 103 847.3 0 103 18 52 847.3 0 52 19 81 847.3 0 81 20 49 847.3 0 49 21 62 847.3 0 62 22 69 847.3 0 69 23 79 847.3 0 79 24 83 847.3 0 83 25 59 847.3 0 59 26 40 847.3 0 40 27 36 847.3 0 36 28 61 847.3 0 61 29 68 847.3 0 68 30 36 847.3 0 36 31 65 847.3 0 65 32 62 847.3 0 62 33 51 847.3 0 51 34 58 847.3 0 58 35 64 847.3 0 64 36 75 847.3 0 75 37 84 847.3 0 84 38 65 847.3 0 65 39 81 847.3 0 81 40 112 847.3 0 112 41 70 847.3 0 70 42 56 847.3 0 56 43 54 847.3 0 54 44 38 847.3 0 38 45 54 847.3 0 54 46 66 847.3 0 66 47 67 847.3 0 67 48 49 847.3 0 49 49 50 847.3 0 50 50 58 847.3 0 58 51 94 847.3 0 94 52 87 847.3 0 87 53 111 847.3 0 111 54 71 847.3 0 71 55 59 847.3 0 59 56 40 847.3 0 40 57 46 847.3 0 46 58 75 847.3 0 75 59 54 847.3 0 54 60 64 847.3 0 64 61 79 847.3 0 79 62 97 847.3 0 97 63 80 847.3 0 80 64 69 847.3 0 69 65 53 847.3 0 53 66 62 847.3 0 62 67 53 847.3 0 53 68 53 847.3 0 53 69 74 847.3 0 74 70 52 847.3 0 52 71 61 847.3 0 61 72 58 847.3 0 58 73 50 847.3 0 50 74 65 847.3 0 65 75 91 847.3 0 91 76 97 847.3 0 97 77 94 847.3 0 94 78 76 847.3 0 76 79 55 847.3 0 55 80 74 847.3 0 74 81 91 847.3 0 91 82 90 847.3 0 90 83 72 847.3 0 72 84 63 847.3 0 63 85 75 847.3 0 75 86 65 847.3 0 65 87 72 847.3 0 72 88 58 847.3 0 58 89 85 847.3 0 85 90 74 847.3 0 74 91 69 847.3 0 69 92 100 847.3 0 100 93 161 847.3 0 161 94 117 847.3 0 117 95 79 847.3 0 79 96 67 847.3 0 67 97 61 847.3 0 61 98 67 847.3 0 67 99 109 847.3 0 109 100 175 847.3 0 175 101 100 847.3 0 100 102 74 847.3 0 74 103 61 847.3 0 61 104 70 847.3 0 70 105 72 847.3 0 72 106 58 847.3 0 58 107 57 847.3 0 57 108 53 847.3 0 53 109 75 847.3 0 75 110 76 847.3 0 76 111 79 847.3 0 79 112 54 847.3 0 54 113 58 847.3 0 58 114 86 847.3 0 86 115 80 847.3 0 80 116 82 847.3 0 82 117 201 847.3 0 201 118 88 847.3 0 88 119 67 847.3 0 67 120 71 847.3 0 71 121 81 847.3 0 81 122 91 847.3 0 91 123 65 847.3 0 65 124 60 847.3 0 60 125 85 847.3 0 85 126 67 847.3 0 67 127 79 847.3 0 79 128 77 847.3 0 77 129 55 847.3 0 55 130 65 847.3 0 65 131 109 847.3 0 109 132 94 847.3 0 94 133 104 847.3 0 104 134 80 847.3 0 80 135 213 847.3 0 213 136 138 847.3 0 138 137 486 847.3 0 486 138 648 847.3 0 648 139 633 847.3 0 633 140 763 847.3 0 763 141 82 847.3 0 82 142 89 847.3 0 89 143 100 847.3 0 100 144 107 847.3 0 107 145 104 847.3 0 104 146 79 847.3 0 79 147 62 847.3 0 62 148 66 847.3 0 66 149 71 847.3 0 71 150 69 847.3 0 69 151 81 847.3 0 81 152 59 847.3 0 59 153 78 847.3 0 78 154 61 847.3 0 61 155 58 847.3 0 58 156 72 847.3 0 72 157 78 847.3 0 78 158 65 847.3 0 65 159 89 847.3 0 89 160 86 847.3 0 86 161 77 847.3 0 77 162 67 847.3 0 67 163 54 847.3 0 54 164 67 847.3 0 67 165 93 847.3 0 93 166 91 847.3 0 91 167 81 847.3 0 81 168 80 847.3 0 80 169 97 847.3 0 97 170 82 847.3 0 82 171 89 847.3 0 89 172 96 847.3 0 96 173 96 847.3 0 96 174 74 847.3 0 74 175 70 847.3 0 70 176 70 847.3 0 70 177 65 847.3 0 65 178 83 847.3 0 83 179 140 847.3 0 140 180 73 847.3 0 73 181 66 847.3 0 66 182 74 847.3 0 74 183 74 847.3 0 74 184 74 847.3 0 74 185 90 847.3 0 90 186 144 847.3 0 144 187 152 847.3 0 152 188 111 847.3 0 111 189 90 847.3 0 90 190 67 847.3 0 67 191 82 847.3 0 82 192 87 847.3 0 87 193 60 847.3 0 60 194 59 847.3 0 59 195 67 847.3 0 67 196 69 847.3 0 69 197 66 847.3 0 66 198 105 847.3 0 105 199 55 847.3 0 55 200 68 847.3 0 68 201 75 847.3 0 75 202 49 847.3 0 49 203 97 847.3 0 97 204 102 847.3 0 102 205 90 847.3 0 90 206 81 847.3 0 81 207 74 847.3 0 74 208 102 847.3 0 102 209 76 847.3 0 76 210 112 847.3 0 112 211 85 847.3 0 85 212 94 847.3 0 94 213 91 847.3 0 91 214 222 847.3 0 222 215 179 847.3 0 179 216 162 847.3 0 162 217 85 847.3 0 85 218 79 847.3 0 79 219 81 847.3 0 81 220 79 847.3 0 79 221 71 847.3 0 71 222 68 847.3 0 68 223 93 847.3 0 93 224 95 847.3 0 95 225 86 847.3 0 86 226 60 847.3 0 60 227 87 847.3 0 87 228 109 847.3 0 109 229 74 847.3 0 74 230 71 847.3 0 71 231 77 847.3 0 77 232 58 847.3 0 58 233 91 847.3 0 91 234 87 847.3 0 87 235 89 847.3 0 89 236 75 847.3 0 75 237 87 847.3 0 87 238 395 847.3 0 395 239 236 847.3 0 236 240 120 847.3 0 120 241 57 847.3 0 57 242 87 847.3 0 87 243 127 847.3 0 127 244 99 847.3 0 99 245 93 847.3 0 93 246 84 847.3 0 84 247 66 847.3 0 66 248 78 847.3 0 78 249 98 847.3 0 98 250 80 847.3 0 80 251 101 847.3 0 101 252 112 847.3 0 112 253 95 847.3 0 95 254 94 847.3 0 94 255 72 847.3 0 72 256 130 847.3 0 130 257 295 847.3 0 295 258 355 847.3 0 355 259 232 847.3 0 232 260 209 847.3 0 209 261 133 847.3 0 133 262 135 847.3 0 135 263 147 847.3 0 147 264 149 847.3 0 149 265 138 847.3 0 138 266 168 847.3 0 168 267 154 847.3 0 154 268 154 847.3 0 154 269 175 847.3 0 175 270 189 847.3 0 189 271 248 847.3 0 248 272 506 847.3 0 506 273 5899 847.3 0 5899 274 4491 847.3 0 4491 275 2523 847.3 0 2523 276 365 847.3 0 365 277 344 847.3 0 344 278 266 847.3 0 266 279 185 847.3 0 185 280 192 847.3 0 192 281 132 847.3 0 132 282 136 847.3 0 136 283 583 847.3 0 583 284 648 847.3 0 648 285 638 847.3 0 638 286 1088 847.3 0 1088 287 944 847.3 0 944 288 593 847.3 0 593 289 183 847.3 0 183 290 221 847.3 0 221 291 213 847.3 0 213 292 370 847.3 0 370 293 1191 847.3 0 1191 294 1378 847.3 0 1378 295 1571 847.3 0 1571 296 362 847.3 0 362 297 647 847.3 0 647 298 2007 847.3 0 2007 299 3334 847.3 0 3334 300 3750 847.3 0 3750 301 3586 847.3 0 3586 Finished in 2.98 s (73 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 40,566 Reads with adapters: 11,262 (27.8%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 11,246 (27.7%) Total basepairs processed: 10,078,238 bp Total written (filtered): 3,057,239 bp (30.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11262 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 10 0.0 3 0 0 8 0 2 19 184 0.0 3 0 148 20 8 8 20 10927 0.0 4 10272 523 99 25 8 21 39 0.0 4 9 24 2 2 2 22 14 0.0 4 3 1 0 1 9 23 39 0.0 4 4 2 1 23 9 24 2 0.0 4 0 0 0 1 1 26 3 0.0 4 0 0 0 2 1 29 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 83 2 0.0 4 0 0 0 0 2 94 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 19 0.0 4 0 0 0 0 19 158 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 2 0.0 4 0 0 0 0 2 233 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 248 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.71 s (63 us/read; 0.95 M reads/minute). === Summary === Total reads processed: 11,246 Reads with adapters: 2,460 (21.9%) Reads written (passing filters): 11,246 (100.0%) Total basepairs processed: 3,057,239 bp Total written (filtered): 3,031,121 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2460 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.5% C: 5.5% G: 89.8% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 175.7 0 8 4 1 43.9 0 1 5 2 11.0 0 2 6 1 2.7 0 1 9 678 0.0 0 49 629 10 1271 0.0 1 85 1186 11 432 0.0 1 8 424 12 9 0.0 1 0 9 13 2 0.0 1 0 2 21 1 0.0 1 1 22 42 0.0 1 31 11 23 4 0.0 1 4 24 2 0.0 1 2 138 1 0.0 1 0 1 150 1 0.0 1 0 1 151 1 0.0 1 1 208 3 0.0 1 1 2 228 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.