This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 163.86 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 83,833 (2.4%) Reads that were too short: 46,721 (1.3%) Reads written (passing filters): 37,112 (1.1%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 9,512,608 bp (0.9%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 83833 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 215 847.3 0 215 7 363 847.3 0 363 8 11351 847.3 0 11351 9 12334 847.3 0 12334 10 67 847.3 0 67 11 42 847.3 0 42 12 55 847.3 0 55 13 92 847.3 0 92 14 40 847.3 0 40 15 40 847.3 0 40 16 46 847.3 0 46 17 56 847.3 0 56 18 31 847.3 0 31 19 43 847.3 0 43 20 51 847.3 0 51 21 51 847.3 0 51 22 37 847.3 0 37 23 42 847.3 0 42 24 60 847.3 0 60 25 56 847.3 0 56 26 31 847.3 0 31 27 36 847.3 0 36 28 55 847.3 0 55 29 49 847.3 0 49 30 41 847.3 0 41 31 49 847.3 0 49 32 49 847.3 0 49 33 37 847.3 0 37 34 46 847.3 0 46 35 47 847.3 0 47 36 56 847.3 0 56 37 88 847.3 0 88 38 48 847.3 0 48 39 35 847.3 0 35 40 233 847.3 0 233 41 60 847.3 0 60 42 65 847.3 0 65 43 41 847.3 0 41 44 56 847.3 0 56 45 58 847.3 0 58 46 55 847.3 0 55 47 40 847.3 0 40 48 39 847.3 0 39 49 37 847.3 0 37 50 52 847.3 0 52 51 64 847.3 0 64 52 53 847.3 0 53 53 62 847.3 0 62 54 64 847.3 0 64 55 59 847.3 0 59 56 45 847.3 0 45 57 43 847.3 0 43 58 46 847.3 0 46 59 35 847.3 0 35 60 45 847.3 0 45 61 55 847.3 0 55 62 56 847.3 0 56 63 55 847.3 0 55 64 46 847.3 0 46 65 78 847.3 0 78 66 70 847.3 0 70 67 46 847.3 0 46 68 79 847.3 0 79 69 52 847.3 0 52 70 42 847.3 0 42 71 41 847.3 0 41 72 59 847.3 0 59 73 47 847.3 0 47 74 66 847.3 0 66 75 65 847.3 0 65 76 49 847.3 0 49 77 51 847.3 0 51 78 54 847.3 0 54 79 61 847.3 0 61 80 50 847.3 0 50 81 73 847.3 0 73 82 62 847.3 0 62 83 50 847.3 0 50 84 38 847.3 0 38 85 54 847.3 0 54 86 44 847.3 0 44 87 43 847.3 0 43 88 36 847.3 0 36 89 41 847.3 0 41 90 69 847.3 0 69 91 47 847.3 0 47 92 78 847.3 0 78 93 68 847.3 0 68 94 85 847.3 0 85 95 69 847.3 0 69 96 60 847.3 0 60 97 60 847.3 0 60 98 68 847.3 0 68 99 73 847.3 0 73 100 85 847.3 0 85 101 92 847.3 0 92 102 57 847.3 0 57 103 53 847.3 0 53 104 75 847.3 0 75 105 69 847.3 0 69 106 46 847.3 0 46 107 52 847.3 0 52 108 38 847.3 0 38 109 62 847.3 0 62 110 72 847.3 0 72 111 46 847.3 0 46 112 34 847.3 0 34 113 43 847.3 0 43 114 58 847.3 0 58 115 56 847.3 0 56 116 55 847.3 0 55 117 169 847.3 0 169 118 72 847.3 0 72 119 58 847.3 0 58 120 60 847.3 0 60 121 62 847.3 0 62 122 54 847.3 0 54 123 41 847.3 0 41 124 53 847.3 0 53 125 65 847.3 0 65 126 47 847.3 0 47 127 72 847.3 0 72 128 48 847.3 0 48 129 43 847.3 0 43 130 60 847.3 0 60 131 71 847.3 0 71 132 58 847.3 0 58 133 61 847.3 0 61 134 60 847.3 0 60 135 227 847.3 0 227 136 74 847.3 0 74 137 222 847.3 0 222 138 405 847.3 0 405 139 342 847.3 0 342 140 556 847.3 0 556 141 69 847.3 0 69 142 42 847.3 0 42 143 66 847.3 0 66 144 63 847.3 0 63 145 66 847.3 0 66 146 58 847.3 0 58 147 40 847.3 0 40 148 68 847.3 0 68 149 48 847.3 0 48 150 58 847.3 0 58 151 54 847.3 0 54 152 66 847.3 0 66 153 59 847.3 0 59 154 72 847.3 0 72 155 45 847.3 0 45 156 63 847.3 0 63 157 63 847.3 0 63 158 51 847.3 0 51 159 63 847.3 0 63 160 60 847.3 0 60 161 64 847.3 0 64 162 56 847.3 0 56 163 52 847.3 0 52 164 77 847.3 0 77 165 52 847.3 0 52 166 53 847.3 0 53 167 52 847.3 0 52 168 67 847.3 0 67 169 53 847.3 0 53 170 52 847.3 0 52 171 90 847.3 0 90 172 60 847.3 0 60 173 86 847.3 0 86 174 39 847.3 0 39 175 53 847.3 0 53 176 46 847.3 0 46 177 70 847.3 0 70 178 98 847.3 0 98 179 100 847.3 0 100 180 68 847.3 0 68 181 57 847.3 0 57 182 55 847.3 0 55 183 61 847.3 0 61 184 71 847.3 0 71 185 61 847.3 0 61 186 127 847.3 0 127 187 106 847.3 0 106 188 86 847.3 0 86 189 72 847.3 0 72 190 50 847.3 0 50 191 43 847.3 0 43 192 51 847.3 0 51 193 58 847.3 0 58 194 53 847.3 0 53 195 70 847.3 0 70 196 60 847.3 0 60 197 67 847.3 0 67 198 68 847.3 0 68 199 80 847.3 0 80 200 76 847.3 0 76 201 74 847.3 0 74 202 61 847.3 0 61 203 75 847.3 0 75 204 65 847.3 0 65 205 47 847.3 0 47 206 66 847.3 0 66 207 61 847.3 0 61 208 93 847.3 0 93 209 64 847.3 0 64 210 83 847.3 0 83 211 70 847.3 0 70 212 64 847.3 0 64 213 66 847.3 0 66 214 95 847.3 0 95 215 97 847.3 0 97 216 86 847.3 0 86 217 83 847.3 0 83 218 77 847.3 0 77 219 66 847.3 0 66 220 55 847.3 0 55 221 65 847.3 0 65 222 49 847.3 0 49 223 56 847.3 0 56 224 64 847.3 0 64 225 58 847.3 0 58 226 63 847.3 0 63 227 72 847.3 0 72 228 80 847.3 0 80 229 54 847.3 0 54 230 64 847.3 0 64 231 63 847.3 0 63 232 50 847.3 0 50 233 68 847.3 0 68 234 67 847.3 0 67 235 73 847.3 0 73 236 75 847.3 0 75 237 75 847.3 0 75 238 204 847.3 0 204 239 152 847.3 0 152 240 82 847.3 0 82 241 66 847.3 0 66 242 81 847.3 0 81 243 69 847.3 0 69 244 78 847.3 0 78 245 59 847.3 0 59 246 58 847.3 0 58 247 63 847.3 0 63 248 84 847.3 0 84 249 55 847.3 0 55 250 63 847.3 0 63 251 82 847.3 0 82 252 58 847.3 0 58 253 56 847.3 0 56 254 45 847.3 0 45 255 49 847.3 0 49 256 77 847.3 0 77 257 120 847.3 0 120 258 127 847.3 0 127 259 115 847.3 0 115 260 160 847.3 0 160 261 89 847.3 0 89 262 127 847.3 0 127 263 168 847.3 0 168 264 90 847.3 0 90 265 117 847.3 0 117 266 123 847.3 0 123 267 128 847.3 0 128 268 129 847.3 0 129 269 127 847.3 0 127 270 157 847.3 0 157 271 219 847.3 0 219 272 388 847.3 0 388 273 6396 847.3 0 6396 274 5012 847.3 0 5012 275 2473 847.3 0 2473 276 244 847.3 0 244 277 136 847.3 0 136 278 104 847.3 0 104 279 158 847.3 0 158 280 155 847.3 0 155 281 107 847.3 0 107 282 100 847.3 0 100 283 278 847.3 0 278 284 316 847.3 0 316 285 389 847.3 0 389 286 588 847.3 0 588 287 572 847.3 0 572 288 333 847.3 0 333 289 110 847.3 0 110 290 176 847.3 0 176 291 152 847.3 0 152 292 295 847.3 0 295 293 1303 847.3 0 1303 294 1508 847.3 0 1508 295 1655 847.3 0 1655 296 307 847.3 0 307 297 583 847.3 0 583 298 1833 847.3 0 1833 299 3999 847.3 0 3999 300 5283 847.3 0 5283 301 5821 847.3 0 5821 Finished in 2.62 s (71 us/read; 0.85 M reads/minute). === Summary === Total reads processed: 37,112 Reads with adapters: 11,271 (30.4%) Reads that were too short: 41 (0.1%) Reads written (passing filters): 11,230 (30.3%) Total basepairs processed: 9,512,608 bp Total written (filtered): 3,097,164 bp (32.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 11271 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 93 0.0 3 0 92 1 17 11066 0.0 3 10859 200 7 18 33 0.0 3 5 28 19 5 0.0 3 0 0 5 20 16 0.0 3 0 0 0 16 29 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 4 0.0 3 0 0 0 4 60 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 1 183 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 217 34 0.0 3 0 1 1 32 280 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.64 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 11,230 Reads with adapters: 60 (0.5%) Reads written (passing filters): 11,230 (100.0%) Total basepairs processed: 3,097,164 bp Total written (filtered): 3,096,966 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 60 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 75.0% C: 10.0% G: 10.0% T: 5.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 50 175.5 0 50 4 8 43.9 0 8 5 1 11.0 0 1 11 1 0.0 1 0 1