This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 166.09 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 137,217 (4.0%) Reads that were too short: 23,292 (0.7%) Reads written (passing filters): 113,925 (3.3%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 30,339,076 bp (2.9%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 137217 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 6022 847.3 0 6022 7 36782 847.3 0 36782 8 43305 847.3 0 43305 9 92 847.3 0 92 10 49 847.3 0 49 11 54 847.3 0 54 12 51 847.3 0 51 13 52 847.3 0 52 14 56 847.3 0 56 15 50 847.3 0 50 16 23 847.3 0 23 17 30 847.3 0 30 18 27 847.3 0 27 19 31 847.3 0 31 20 40 847.3 0 40 21 38 847.3 0 38 22 47 847.3 0 47 23 51 847.3 0 51 24 41 847.3 0 41 25 39 847.3 0 39 26 43 847.3 0 43 27 38 847.3 0 38 28 43 847.3 0 43 29 44 847.3 0 44 30 51 847.3 0 51 31 76 847.3 0 76 32 48 847.3 0 48 33 57 847.3 0 57 34 48 847.3 0 48 35 45 847.3 0 45 36 62 847.3 0 62 37 81 847.3 0 81 38 34 847.3 0 34 39 64 847.3 0 64 40 77 847.3 0 77 41 54 847.3 0 54 42 40 847.3 0 40 43 83 847.3 0 83 44 66 847.3 0 66 45 62 847.3 0 62 46 63 847.3 0 63 47 78 847.3 0 78 48 109 847.3 0 109 49 96 847.3 0 96 50 1845 847.3 0 1845 51 2882 847.3 0 2882 52 888 847.3 0 888 53 34 847.3 0 34 54 40 847.3 0 40 55 24 847.3 0 24 56 23 847.3 0 23 57 43 847.3 0 43 58 36 847.3 0 36 59 35 847.3 0 35 60 56 847.3 0 56 61 66 847.3 0 66 62 30 847.3 0 30 63 97 847.3 0 97 64 50 847.3 0 50 65 68 847.3 0 68 66 54 847.3 0 54 67 43 847.3 0 43 68 44 847.3 0 44 69 41 847.3 0 41 70 43 847.3 0 43 71 44 847.3 0 44 72 60 847.3 0 60 73 73 847.3 0 73 74 342 847.3 0 342 75 305 847.3 0 305 76 371 847.3 0 371 77 48 847.3 0 48 78 102 847.3 0 102 79 50 847.3 0 50 80 56 847.3 0 56 81 56 847.3 0 56 82 54 847.3 0 54 83 42 847.3 0 42 84 35 847.3 0 35 85 46 847.3 0 46 86 35 847.3 0 35 87 40 847.3 0 40 88 36 847.3 0 36 89 44 847.3 0 44 90 61 847.3 0 61 91 43 847.3 0 43 92 38 847.3 0 38 93 46 847.3 0 46 94 33 847.3 0 33 95 38 847.3 0 38 96 45 847.3 0 45 97 33 847.3 0 33 98 66 847.3 0 66 99 61 847.3 0 61 100 49 847.3 0 49 101 68 847.3 0 68 102 71 847.3 0 71 103 56 847.3 0 56 104 68 847.3 0 68 105 45 847.3 0 45 106 46 847.3 0 46 107 58 847.3 0 58 108 37 847.3 0 37 109 53 847.3 0 53 110 51 847.3 0 51 111 57 847.3 0 57 112 77 847.3 0 77 113 58 847.3 0 58 114 88 847.3 0 88 115 104 847.3 0 104 116 77 847.3 0 77 117 120 847.3 0 120 118 81 847.3 0 81 119 92 847.3 0 92 120 80 847.3 0 80 121 83 847.3 0 83 122 90 847.3 0 90 123 74 847.3 0 74 124 120 847.3 0 120 125 1463 847.3 0 1463 126 1641 847.3 0 1641 127 412 847.3 0 412 128 72 847.3 0 72 129 82 847.3 0 82 130 237 847.3 0 237 131 164 847.3 0 164 132 170 847.3 0 170 133 48 847.3 0 48 134 40 847.3 0 40 135 70 847.3 0 70 136 191 847.3 0 191 137 73 847.3 0 73 138 61 847.3 0 61 139 50 847.3 0 50 140 42 847.3 0 42 141 39 847.3 0 39 142 50 847.3 0 50 143 40 847.3 0 40 144 63 847.3 0 63 145 60 847.3 0 60 146 42 847.3 0 42 147 50 847.3 0 50 148 46 847.3 0 46 149 38 847.3 0 38 150 45 847.3 0 45 151 66 847.3 0 66 152 49 847.3 0 49 153 56 847.3 0 56 154 42 847.3 0 42 155 34 847.3 0 34 156 42 847.3 0 42 157 71 847.3 0 71 158 38 847.3 0 38 159 47 847.3 0 47 160 48 847.3 0 48 161 46 847.3 0 46 162 53 847.3 0 53 163 54 847.3 0 54 164 359 847.3 0 359 165 288 847.3 0 288 166 317 847.3 0 317 167 57 847.3 0 57 168 86 847.3 0 86 169 42 847.3 0 42 170 69 847.3 0 69 171 55 847.3 0 55 172 52 847.3 0 52 173 60 847.3 0 60 174 68 847.3 0 68 175 43 847.3 0 43 176 58 847.3 0 58 177 79 847.3 0 79 178 68 847.3 0 68 179 82 847.3 0 82 180 53 847.3 0 53 181 87 847.3 0 87 182 70 847.3 0 70 183 97 847.3 0 97 184 80 847.3 0 80 185 69 847.3 0 69 186 79 847.3 0 79 187 95 847.3 0 95 188 63 847.3 0 63 189 96 847.3 0 96 190 89 847.3 0 89 191 337 847.3 0 337 192 283 847.3 0 283 193 152 847.3 0 152 194 161 847.3 0 161 195 157 847.3 0 157 196 1129 847.3 0 1129 197 1547 847.3 0 1547 198 1509 847.3 0 1509 199 723 847.3 0 723 200 314 847.3 0 314 201 127 847.3 0 127 202 167 847.3 0 167 203 172 847.3 0 172 204 1729 847.3 0 1729 205 995 847.3 0 995 206 2812 847.3 0 2812 207 113 847.3 0 113 208 115 847.3 0 115 209 103 847.3 0 103 210 105 847.3 0 105 211 111 847.3 0 111 212 78 847.3 0 78 213 92 847.3 0 92 214 89 847.3 0 89 215 100 847.3 0 100 216 763 847.3 0 763 217 774 847.3 0 774 218 417 847.3 0 417 219 166 847.3 0 166 220 108 847.3 0 108 221 84 847.3 0 84 222 86 847.3 0 86 223 92 847.3 0 92 224 79 847.3 0 79 225 92 847.3 0 92 226 108 847.3 0 108 227 66 847.3 0 66 228 90 847.3 0 90 229 66 847.3 0 66 230 79 847.3 0 79 231 56 847.3 0 56 232 55 847.3 0 55 233 51 847.3 0 51 234 91 847.3 0 91 235 148 847.3 0 148 236 51 847.3 0 51 237 79 847.3 0 79 238 85 847.3 0 85 239 138 847.3 0 138 240 124 847.3 0 124 241 198 847.3 0 198 242 89 847.3 0 89 243 80 847.3 0 80 244 67 847.3 0 67 245 70 847.3 0 70 246 100 847.3 0 100 247 640 847.3 0 640 248 1029 847.3 0 1029 249 945 847.3 0 945 250 606 847.3 0 606 251 135 847.3 0 135 252 91 847.3 0 91 253 77 847.3 0 77 254 83 847.3 0 83 255 75 847.3 0 75 256 71 847.3 0 71 257 89 847.3 0 89 258 76 847.3 0 76 259 95 847.3 0 95 260 226 847.3 0 226 261 80 847.3 0 80 262 140 847.3 0 140 263 83 847.3 0 83 264 79 847.3 0 79 265 73 847.3 0 73 266 70 847.3 0 70 267 63 847.3 0 63 268 58 847.3 0 58 269 473 847.3 0 473 270 404 847.3 0 404 271 607 847.3 0 607 272 67 847.3 0 67 273 74 847.3 0 74 274 71 847.3 0 71 275 73 847.3 0 73 276 70 847.3 0 70 277 82 847.3 0 82 278 93 847.3 0 93 279 155 847.3 0 155 280 120 847.3 0 120 281 79 847.3 0 79 282 74 847.3 0 74 283 66 847.3 0 66 284 65 847.3 0 65 285 81 847.3 0 81 286 123 847.3 0 123 287 94 847.3 0 94 288 84 847.3 0 84 289 410 847.3 0 410 290 854 847.3 0 854 291 1059 847.3 0 1059 292 480 847.3 0 480 293 210 847.3 0 210 294 93 847.3 0 93 295 62 847.3 0 62 296 85 847.3 0 85 297 167 847.3 0 167 298 158 847.3 0 158 299 251 847.3 0 251 300 170 847.3 0 170 301 121 847.3 0 121 Finished in 8.14 s (71 us/read; 0.84 M reads/minute). === Summary === Total reads processed: 113,925 Reads with adapters: 37,656 (33.1%) Reads that were too short: 76 (0.1%) Reads written (passing filters): 37,580 (33.0%) Total basepairs processed: 30,339,076 bp Total written (filtered): 10,397,624 bp (34.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 37656 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 8 0.0 3 0 0 8 16 296 0.0 3 0 292 4 17 37055 0.0 3 36323 713 17 2 18 81 0.0 3 20 58 3 19 18 0.0 3 0 0 15 3 20 34 0.0 3 1 0 0 33 23 1 0.0 3 0 0 0 1 25 1 0.0 3 0 1 26 2 0.0 3 0 0 0 2 27 7 0.0 3 0 0 0 7 30 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 35 3 0.0 3 0 0 0 3 37 2 0.0 3 0 0 0 2 46 22 0.0 3 0 0 0 22 48 3 0.0 3 0 0 0 3 62 5 0.0 3 0 0 1 4 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 5 0.0 3 0 0 0 5 78 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 87 16 0.0 3 0 0 0 16 88 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 13 0.0 3 0 0 1 12 91 4 0.0 3 0 0 0 4 92 1 0.0 3 0 0 1 96 1 0.0 3 0 0 0 1 97 3 0.0 3 0 0 0 3 99 1 0.0 3 0 0 0 1 100 3 0.0 3 0 0 0 3 104 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 117 9 0.0 3 0 0 0 9 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 1 141 4 0.0 3 0 0 0 4 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 1 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 5 0.0 3 0 0 0 5 160 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 209 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.10 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 37,580 Reads with adapters: 113 (0.3%) Reads written (passing filters): 37,580 (100.0%) Total basepairs processed: 10,397,624 bp Total written (filtered): 10,397,255 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 113 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 44.2% C: 15.9% G: 17.7% T: 22.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 89 587.2 0 89 4 21 146.8 0 21 6 3 9.2 0 3