This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 162.53 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 161,132 (4.6%) Reads that were too short: 122,353 (3.5%) Reads written (passing filters): 38,779 (1.1%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 6,734,796 bp (0.6%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 161132 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 424 847.3 0 424 7 175 847.3 0 175 8 27 847.3 0 27 9 125 847.3 0 125 10 39 847.3 0 39 11 30 847.3 0 30 12 33 847.3 0 33 13 30 847.3 0 30 14 25 847.3 0 25 15 47 847.3 0 47 16 53 847.3 0 53 17 71 847.3 0 71 18 51 847.3 0 51 19 35 847.3 0 35 20 39 847.3 0 39 21 26 847.3 0 26 22 48 847.3 0 48 23 56 847.3 0 56 24 38 847.3 0 38 25 41 847.3 0 41 26 20 847.3 0 20 27 476 847.3 0 476 28 318 847.3 0 318 29 39 847.3 0 39 30 43 847.3 0 43 31 31 847.3 0 31 32 38 847.3 0 38 33 63 847.3 0 63 34 42 847.3 0 42 35 38 847.3 0 38 36 36 847.3 0 36 37 35 847.3 0 35 38 46 847.3 0 46 39 34 847.3 0 34 40 81 847.3 0 81 41 80 847.3 0 80 42 49 847.3 0 49 43 43 847.3 0 43 44 34 847.3 0 34 45 39 847.3 0 39 46 49 847.3 0 49 47 39 847.3 0 39 48 49 847.3 0 49 49 49 847.3 0 49 50 57 847.3 0 57 51 63 847.3 0 63 52 55 847.3 0 55 53 53 847.3 0 53 54 61 847.3 0 61 55 74 847.3 0 74 56 99 847.3 0 99 57 679 847.3 0 679 58 740 847.3 0 740 59 2070 847.3 0 2070 60 47 847.3 0 47 61 38 847.3 0 38 62 40 847.3 0 40 63 45 847.3 0 45 64 42 847.3 0 42 65 43 847.3 0 43 66 30 847.3 0 30 67 46 847.3 0 46 68 57 847.3 0 57 69 44 847.3 0 44 70 27 847.3 0 27 71 36 847.3 0 36 72 50 847.3 0 50 73 38 847.3 0 38 74 49 847.3 0 49 75 32 847.3 0 32 76 44 847.3 0 44 77 55 847.3 0 55 78 58 847.3 0 58 79 65 847.3 0 65 80 95 847.3 0 95 81 56 847.3 0 56 82 65 847.3 0 65 83 94 847.3 0 94 84 114 847.3 0 114 85 72 847.3 0 72 86 101 847.3 0 101 87 91 847.3 0 91 88 204 847.3 0 204 89 164 847.3 0 164 90 284 847.3 0 284 91 148 847.3 0 148 92 134 847.3 0 134 93 83 847.3 0 83 94 57 847.3 0 57 95 60 847.3 0 60 96 59 847.3 0 59 97 60 847.3 0 60 98 96 847.3 0 96 99 81 847.3 0 81 100 76 847.3 0 76 101 67 847.3 0 67 102 71 847.3 0 71 103 73 847.3 0 73 104 110 847.3 0 110 105 151 847.3 0 151 106 134 847.3 0 134 107 169 847.3 0 169 108 122 847.3 0 122 109 95 847.3 0 95 110 92 847.3 0 92 111 68 847.3 0 68 112 59 847.3 0 59 113 71 847.3 0 71 114 77 847.3 0 77 115 93 847.3 0 93 116 77 847.3 0 77 117 130 847.3 0 130 118 67 847.3 0 67 119 79 847.3 0 79 120 108 847.3 0 108 121 150 847.3 0 150 122 710 847.3 0 710 123 548 847.3 0 548 124 969 847.3 0 969 125 65 847.3 0 65 126 75 847.3 0 75 127 84 847.3 0 84 128 88 847.3 0 88 129 75 847.3 0 75 130 106 847.3 0 106 131 90 847.3 0 90 132 99 847.3 0 99 133 98 847.3 0 98 134 124 847.3 0 124 135 178 847.3 0 178 136 118 847.3 0 118 137 160 847.3 0 160 138 84 847.3 0 84 139 130 847.3 0 130 140 118 847.3 0 118 141 256 847.3 0 256 142 317 847.3 0 317 143 423 847.3 0 423 144 108 847.3 0 108 145 108 847.3 0 108 146 127 847.3 0 127 147 203 847.3 0 203 148 331 847.3 0 331 149 435 847.3 0 435 150 264 847.3 0 264 151 2831 847.3 0 2831 152 2732 847.3 0 2732 153 5705 847.3 0 5705 154 126 847.3 0 126 155 173 847.3 0 173 156 137 847.3 0 137 157 436 847.3 0 436 158 390 847.3 0 390 159 650 847.3 0 650 160 120 847.3 0 120 161 158 847.3 0 158 162 238 847.3 0 238 163 229 847.3 0 229 164 106 847.3 0 106 165 70 847.3 0 70 166 80 847.3 0 80 167 73 847.3 0 73 168 123 847.3 0 123 169 105 847.3 0 105 170 141 847.3 0 141 171 119 847.3 0 119 172 63 847.3 0 63 173 59 847.3 0 59 174 80 847.3 0 80 175 92 847.3 0 92 176 182 847.3 0 182 177 191 847.3 0 191 178 113 847.3 0 113 179 125 847.3 0 125 180 81 847.3 0 81 181 120 847.3 0 120 182 287 847.3 0 287 183 200 847.3 0 200 184 207 847.3 0 207 185 144 847.3 0 144 186 320 847.3 0 320 187 179 847.3 0 179 188 165 847.3 0 165 189 180 847.3 0 180 190 83 847.3 0 83 191 63 847.3 0 63 192 72 847.3 0 72 193 141 847.3 0 141 194 140 847.3 0 140 195 243 847.3 0 243 196 456 847.3 0 456 197 159 847.3 0 159 198 200 847.3 0 200 199 108 847.3 0 108 200 226 847.3 0 226 201 589 847.3 0 589 202 448 847.3 0 448 203 329 847.3 0 329 204 203 847.3 0 203 205 208 847.3 0 208 206 372 847.3 0 372 207 319 847.3 0 319 208 477 847.3 0 477 209 1016 847.3 0 1016 210 1082 847.3 0 1082 211 1812 847.3 0 1812 212 1903 847.3 0 1903 213 20850 847.3 0 20850 214 20500 847.3 0 20500 215 52256 847.3 0 52256 216 2174 847.3 0 2174 217 125 847.3 0 125 218 119 847.3 0 119 219 145 847.3 0 145 220 211 847.3 0 211 221 887 847.3 0 887 222 1330 847.3 0 1330 223 2459 847.3 0 2459 224 354 847.3 0 354 225 90 847.3 0 90 226 93 847.3 0 93 227 121 847.3 0 121 228 84 847.3 0 84 229 76 847.3 0 76 230 95 847.3 0 95 231 166 847.3 0 166 232 164 847.3 0 164 233 109 847.3 0 109 234 109 847.3 0 109 235 178 847.3 0 178 236 127 847.3 0 127 237 95 847.3 0 95 238 71 847.3 0 71 239 84 847.3 0 84 240 100 847.3 0 100 241 106 847.3 0 106 242 118 847.3 0 118 243 149 847.3 0 149 244 170 847.3 0 170 245 209 847.3 0 209 246 177 847.3 0 177 247 117 847.3 0 117 248 85 847.3 0 85 249 114 847.3 0 114 250 111 847.3 0 111 251 107 847.3 0 107 252 113 847.3 0 113 253 86 847.3 0 86 254 100 847.3 0 100 255 124 847.3 0 124 256 156 847.3 0 156 257 290 847.3 0 290 258 348 847.3 0 348 259 499 847.3 0 499 260 446 847.3 0 446 261 222 847.3 0 222 262 207 847.3 0 207 263 400 847.3 0 400 264 340 847.3 0 340 265 272 847.3 0 272 266 100 847.3 0 100 267 77 847.3 0 77 268 132 847.3 0 132 269 206 847.3 0 206 270 223 847.3 0 223 271 191 847.3 0 191 272 180 847.3 0 180 273 108 847.3 0 108 274 146 847.3 0 146 275 118 847.3 0 118 276 183 847.3 0 183 277 194 847.3 0 194 278 132 847.3 0 132 279 115 847.3 0 115 280 130 847.3 0 130 281 315 847.3 0 315 282 329 847.3 0 329 283 302 847.3 0 302 284 281 847.3 0 281 285 211 847.3 0 211 286 216 847.3 0 216 287 128 847.3 0 128 288 109 847.3 0 109 289 147 847.3 0 147 290 174 847.3 0 174 291 139 847.3 0 139 292 108 847.3 0 108 293 99 847.3 0 99 294 137 847.3 0 137 295 184 847.3 0 184 296 180 847.3 0 180 297 156 847.3 0 156 298 138 847.3 0 138 299 155 847.3 0 155 300 113 847.3 0 113 301 90 847.3 0 90 Finished in 2.10 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 38,779 Reads with adapters: 221 (0.6%) Reads that were too short: 31 (0.1%) Reads written (passing filters): 190 (0.5%) Total basepairs processed: 6,734,796 bp Total written (filtered): 38,054 bp (0.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 221 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 2 0.0 3 0 2 17 93 0.0 3 92 1 18 3 0.0 3 0 1 2 19 5 0.0 3 3 0 1 1 20 1 0.0 3 0 0 0 1 21 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 32 2 0.0 3 0 0 0 2 34 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 130 3 0.0 3 0 0 0 3 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 160 90 0.0 3 0 0 0 90 163 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 201 2 0.0 3 0 0 0 2 213 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 190 Reads with adapters: 7 (3.7%) Reads written (passing filters): 190 (100.0%) Total basepairs processed: 38,054 bp Total written (filtered): 38,032 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 7 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 85.7% C: 14.3% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 6 3.0 0 6 4 1 0.7 0 1