This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 173.62 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 508,099 (14.6%) Reads that were too short: 19,045 (0.5%) Reads written (passing filters): 489,054 (14.1%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 114,788,664 bp (11.0%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 508099 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1870 847.3 0 1870 7 3341 847.3 0 3341 8 66549 847.3 0 66549 9 54231 847.3 0 54231 10 138 847.3 0 138 11 77 847.3 0 77 12 108 847.3 0 108 13 87 847.3 0 87 14 71 847.3 0 71 15 96 847.3 0 96 16 70 847.3 0 70 17 96 847.3 0 96 18 114 847.3 0 114 19 118 847.3 0 118 20 130 847.3 0 130 21 110 847.3 0 110 22 162 847.3 0 162 23 294 847.3 0 294 24 172 847.3 0 172 25 106 847.3 0 106 26 108 847.3 0 108 27 110 847.3 0 110 28 88 847.3 0 88 29 133 847.3 0 133 30 131 847.3 0 131 31 117 847.3 0 117 32 117 847.3 0 117 33 118 847.3 0 118 34 139 847.3 0 139 35 111 847.3 0 111 36 194 847.3 0 194 37 190 847.3 0 190 38 345 847.3 0 345 39 197 847.3 0 197 40 193 847.3 0 193 41 133 847.3 0 133 42 321 847.3 0 321 43 174 847.3 0 174 44 232 847.3 0 232 45 347 847.3 0 347 46 480 847.3 0 480 47 647 847.3 0 647 48 1295 847.3 0 1295 49 2331 847.3 0 2331 50 623 847.3 0 623 51 718 847.3 0 718 52 1142 847.3 0 1142 53 853 847.3 0 853 54 754 847.3 0 754 55 1285 847.3 0 1285 56 1067 847.3 0 1067 57 1448 847.3 0 1448 58 1451 847.3 0 1451 59 2686 847.3 0 2686 60 3121 847.3 0 3121 61 6500 847.3 0 6500 62 124015 847.3 0 124015 63 67549 847.3 0 67549 64 37891 847.3 0 37891 65 745 847.3 0 745 66 110 847.3 0 110 67 99 847.3 0 99 68 88 847.3 0 88 69 110 847.3 0 110 70 127 847.3 0 127 71 218 847.3 0 218 72 356 847.3 0 356 73 555 847.3 0 555 74 233 847.3 0 233 75 183 847.3 0 183 76 938 847.3 0 938 77 2246 847.3 0 2246 78 2636 847.3 0 2636 79 241 847.3 0 241 80 161 847.3 0 161 81 84 847.3 0 84 82 120 847.3 0 120 83 96 847.3 0 96 84 76 847.3 0 76 85 105 847.3 0 105 86 77 847.3 0 77 87 77 847.3 0 77 88 85 847.3 0 85 89 73 847.3 0 73 90 108 847.3 0 108 91 127 847.3 0 127 92 89 847.3 0 89 93 134 847.3 0 134 94 128 847.3 0 128 95 130 847.3 0 130 96 56 847.3 0 56 97 72 847.3 0 72 98 279 847.3 0 279 99 191 847.3 0 191 100 304 847.3 0 304 101 91 847.3 0 91 102 88 847.3 0 88 103 134 847.3 0 134 104 136 847.3 0 136 105 110 847.3 0 110 106 117 847.3 0 117 107 336 847.3 0 336 108 718 847.3 0 718 109 873 847.3 0 873 110 264 847.3 0 264 111 199 847.3 0 199 112 206 847.3 0 206 113 75 847.3 0 75 114 75 847.3 0 75 115 105 847.3 0 105 116 92 847.3 0 92 117 115 847.3 0 115 118 88 847.3 0 88 119 71 847.3 0 71 120 93 847.3 0 93 121 90 847.3 0 90 122 61 847.3 0 61 123 79 847.3 0 79 124 92 847.3 0 92 125 104 847.3 0 104 126 154 847.3 0 154 127 188 847.3 0 188 128 144 847.3 0 144 129 164 847.3 0 164 130 131 847.3 0 131 131 129 847.3 0 129 132 159 847.3 0 159 133 152 847.3 0 152 134 153 847.3 0 153 135 115 847.3 0 115 136 109 847.3 0 109 137 204 847.3 0 204 138 140 847.3 0 140 139 208 847.3 0 208 140 224 847.3 0 224 141 296 847.3 0 296 142 468 847.3 0 468 143 597 847.3 0 597 144 4413 847.3 0 4413 145 4396 847.3 0 4396 146 9103 847.3 0 9103 147 363 847.3 0 363 148 300 847.3 0 300 149 207 847.3 0 207 150 178 847.3 0 178 151 192 847.3 0 192 152 340 847.3 0 340 153 264 847.3 0 264 154 286 847.3 0 286 155 258 847.3 0 258 156 1361 847.3 0 1361 157 1275 847.3 0 1275 158 2871 847.3 0 2871 159 356 847.3 0 356 160 363 847.3 0 363 161 373 847.3 0 373 162 631 847.3 0 631 163 907 847.3 0 907 164 7098 847.3 0 7098 165 8692 847.3 0 8692 166 15762 847.3 0 15762 167 261 847.3 0 261 168 260 847.3 0 260 169 953 847.3 0 953 170 2648 847.3 0 2648 171 3186 847.3 0 3186 172 1956 847.3 0 1956 173 915 847.3 0 915 174 1505 847.3 0 1505 175 279 847.3 0 279 176 87 847.3 0 87 177 118 847.3 0 118 178 134 847.3 0 134 179 230 847.3 0 230 180 489 847.3 0 489 181 261 847.3 0 261 182 123 847.3 0 123 183 182 847.3 0 182 184 274 847.3 0 274 185 259 847.3 0 259 186 224 847.3 0 224 187 230 847.3 0 230 188 239 847.3 0 239 189 1684 847.3 0 1684 190 2091 847.3 0 2091 191 1585 847.3 0 1585 192 928 847.3 0 928 193 88 847.3 0 88 194 94 847.3 0 94 195 70 847.3 0 70 196 104 847.3 0 104 197 156 847.3 0 156 198 186 847.3 0 186 199 162 847.3 0 162 200 112 847.3 0 112 201 94 847.3 0 94 202 104 847.3 0 104 203 112 847.3 0 112 204 112 847.3 0 112 205 102 847.3 0 102 206 141 847.3 0 141 207 112 847.3 0 112 208 148 847.3 0 148 209 98 847.3 0 98 210 85 847.3 0 85 211 87 847.3 0 87 212 103 847.3 0 103 213 110 847.3 0 110 214 139 847.3 0 139 215 213 847.3 0 213 216 266 847.3 0 266 217 381 847.3 0 381 218 282 847.3 0 282 219 157 847.3 0 157 220 76 847.3 0 76 221 67 847.3 0 67 222 77 847.3 0 77 223 82 847.3 0 82 224 127 847.3 0 127 225 146 847.3 0 146 226 354 847.3 0 354 227 378 847.3 0 378 228 375 847.3 0 375 229 286 847.3 0 286 230 183 847.3 0 183 231 434 847.3 0 434 232 111 847.3 0 111 233 87 847.3 0 87 234 89 847.3 0 89 235 110 847.3 0 110 236 113 847.3 0 113 237 138 847.3 0 138 238 187 847.3 0 187 239 139 847.3 0 139 240 133 847.3 0 133 241 94 847.3 0 94 242 70 847.3 0 70 243 156 847.3 0 156 244 163 847.3 0 163 245 150 847.3 0 150 246 146 847.3 0 146 247 201 847.3 0 201 248 192 847.3 0 192 249 150 847.3 0 150 250 103 847.3 0 103 251 112 847.3 0 112 252 124 847.3 0 124 253 118 847.3 0 118 254 111 847.3 0 111 255 82 847.3 0 82 256 96 847.3 0 96 257 107 847.3 0 107 258 76 847.3 0 76 259 148 847.3 0 148 260 235 847.3 0 235 261 319 847.3 0 319 262 378 847.3 0 378 263 351 847.3 0 351 264 164 847.3 0 164 265 192 847.3 0 192 266 199 847.3 0 199 267 194 847.3 0 194 268 167 847.3 0 167 269 83 847.3 0 83 270 114 847.3 0 114 271 145 847.3 0 145 272 179 847.3 0 179 273 171 847.3 0 171 274 134 847.3 0 134 275 297 847.3 0 297 276 136 847.3 0 136 277 363 847.3 0 363 278 290 847.3 0 290 279 274 847.3 0 274 280 147 847.3 0 147 281 201 847.3 0 201 282 211 847.3 0 211 283 112 847.3 0 112 284 181 847.3 0 181 285 286 847.3 0 286 286 331 847.3 0 331 287 654 847.3 0 654 288 671 847.3 0 671 289 784 847.3 0 784 290 453 847.3 0 453 291 163 847.3 0 163 292 198 847.3 0 198 293 181 847.3 0 181 294 125 847.3 0 125 295 170 847.3 0 170 296 123 847.3 0 123 297 170 847.3 0 170 298 185 847.3 0 185 299 183 847.3 0 183 300 139 847.3 0 139 301 149 847.3 0 149 Finished in 34.39 s (70 us/read; 0.85 M reads/minute). === Summary === Total reads processed: 489,054 Reads with adapters: 69,422 (14.2%) Reads that were too short: 292 (0.1%) Reads written (passing filters): 69,130 (14.1%) Total basepairs processed: 114,788,664 bp Total written (filtered): 17,698,387 bp (15.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 69422 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 3 18 33 0.0 3 0 0 26 7 19 712 0.0 3 0 617 44 35 16 20 57458 0.0 4 54683 2268 354 116 37 21 134 0.0 4 21 102 7 3 1 22 15 0.0 4 2 0 9 1 3 23 201 0.0 4 3 1 0 129 68 24 1 0.0 4 1 25 2 0.0 4 0 0 1 0 1 26 2 0.0 4 0 0 0 0 2 27 1 0.0 4 1 34 21 0.0 4 0 0 0 0 21 37 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 51 4 0.0 4 0 0 0 0 4 52 1 0.0 4 0 0 0 0 1 53 3 0.0 4 1 0 0 0 2 55 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 70 11 0.0 4 0 0 0 0 11 71 8 0.0 4 0 0 0 0 8 72 10339 0.0 4 0 0 0 0 10339 74 1 0.0 4 0 0 0 0 1 75 21 0.0 4 0 0 0 0 21 78 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 85 4 0.0 4 0 0 0 1 3 97 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 147 0.0 4 0 0 0 0 147 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 138 2 0.0 4 0 2 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 148 3 0.0 4 0 0 0 0 3 152 1 0.0 4 0 0 0 0 1 153 7 0.0 4 0 0 0 1 6 154 1 0.0 4 0 0 0 0 1 157 2 0.0 4 0 0 0 0 2 158 2 0.0 4 0 0 0 0 2 161 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 1 165 1 0.0 4 0 0 0 0 1 166 5 0.0 4 0 0 0 1 4 167 4 0.0 4 0 0 0 0 4 168 3 0.0 4 0 0 0 1 2 171 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 173 74 0.0 4 0 0 0 1 73 174 26 0.0 4 0 0 0 1 25 175 2 0.0 4 0 0 0 0 2 176 2 0.0 4 0 0 0 0 2 177 2 0.0 4 0 0 0 0 2 178 1 0.0 4 0 0 0 0 1 179 8 0.0 4 0 0 0 0 8 180 11 0.0 4 0 0 0 0 11 181 15 0.0 4 0 0 0 0 15 182 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 202 5 0.0 4 0 0 0 0 5 203 2 0.0 4 0 0 1 0 1 204 2 0.0 4 0 0 0 0 2 206 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 1 1 216 2 0.0 4 0 0 0 0 2 219 1 0.0 4 0 0 0 0 1 225 2 0.0 4 0 0 0 0 2 226 1 0.0 4 0 0 0 0 1 227 20 0.0 4 0 0 0 0 20 228 6 0.0 4 0 0 0 2 4 229 2 0.0 4 0 0 0 0 2 230 3 0.0 4 0 0 0 0 3 231 1 0.0 4 0 0 0 0 1 233 14 0.0 4 0 0 0 0 14 235 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 237 3 0.0 4 0 0 0 0 3 238 1 0.0 4 0 0 0 0 1 239 4 0.0 4 0 0 0 0 4 240 2 0.0 4 0 0 0 0 2 251 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 263 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 265 2 0.0 4 0 0 0 0 2 267 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 282 3 0.0 4 0 0 0 0 3 288 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.23 s (61 us/read; 0.98 M reads/minute). === Summary === Total reads processed: 69,130 Reads with adapters: 20,256 (29.3%) Reads written (passing filters): 69,130 (100.0%) Total basepairs processed: 17,698,387 bp Total written (filtered): 17,503,414 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 20256 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.6% C: 4.8% G: 92.4% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 67 1080.2 0 67 4 65 270.0 0 65 5 3 67.5 0 3 6 5 16.9 0 5 7 1 4.2 0 1 8 6 1.1 0 6 9 13112 0.3 0 836 12276 10 5722 0.1 1 361 5361 11 1130 0.0 1 25 1105 12 76 0.0 1 1 75 13 16 0.0 1 0 16 14 1 0.0 1 0 1 18 18 0.0 1 14 4 19 1 0.0 1 1 40 1 0.0 1 1 122 1 0.0 1 1 123 1 0.0 1 0 1 138 1 0.0 1 1 150 19 0.0 1 16 3 151 1 0.0 1 1 189 1 0.0 1 0 1 208 8 0.0 1 6 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.