This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 167.16 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,470,393 Reads with adapters: 81,561 (2.4%) Reads that were too short: 11,973 (0.3%) Reads written (passing filters): 69,588 (2.0%) Total basepairs processed: 1,044,588,293 bp Total written (filtered): 14,281,141 bp (1.4%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 81561 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 662 847.3 0 662 7 11597 847.3 0 11597 8 10511 847.3 0 10511 9 106 847.3 0 106 10 96 847.3 0 96 11 73 847.3 0 73 12 84 847.3 0 84 13 73 847.3 0 73 14 95 847.3 0 95 15 86 847.3 0 86 16 78 847.3 0 78 17 73 847.3 0 73 18 96 847.3 0 96 19 128 847.3 0 128 20 167 847.3 0 167 21 201 847.3 0 201 22 88 847.3 0 88 23 94 847.3 0 94 24 80 847.3 0 80 25 73 847.3 0 73 26 116 847.3 0 116 27 76 847.3 0 76 28 78 847.3 0 78 29 73 847.3 0 73 30 86 847.3 0 86 31 109 847.3 0 109 32 80 847.3 0 80 33 89 847.3 0 89 34 111 847.3 0 111 35 89 847.3 0 89 36 93 847.3 0 93 37 92 847.3 0 92 38 162 847.3 0 162 39 117 847.3 0 117 40 154 847.3 0 154 41 96 847.3 0 96 42 86 847.3 0 86 43 81 847.3 0 81 44 87 847.3 0 87 45 106 847.3 0 106 46 93 847.3 0 93 47 80 847.3 0 80 48 112 847.3 0 112 49 105 847.3 0 105 50 104 847.3 0 104 51 88 847.3 0 88 52 98 847.3 0 98 53 99 847.3 0 99 54 82 847.3 0 82 55 69 847.3 0 69 56 97 847.3 0 97 57 121 847.3 0 121 58 97 847.3 0 97 59 97 847.3 0 97 60 89 847.3 0 89 61 80 847.3 0 80 62 88 847.3 0 88 63 85 847.3 0 85 64 85 847.3 0 85 65 92 847.3 0 92 66 85 847.3 0 85 67 79 847.3 0 79 68 110 847.3 0 110 69 101 847.3 0 101 70 78 847.3 0 78 71 110 847.3 0 110 72 97 847.3 0 97 73 94 847.3 0 94 74 82 847.3 0 82 75 94 847.3 0 94 76 122 847.3 0 122 77 132 847.3 0 132 78 312 847.3 0 312 79 222 847.3 0 222 80 93 847.3 0 93 81 130 847.3 0 130 82 92 847.3 0 92 83 79 847.3 0 79 84 85 847.3 0 85 85 90 847.3 0 90 86 104 847.3 0 104 87 103 847.3 0 103 88 98 847.3 0 98 89 150 847.3 0 150 90 102 847.3 0 102 91 104 847.3 0 104 92 126 847.3 0 126 93 313 847.3 0 313 94 294 847.3 0 294 95 155 847.3 0 155 96 212 847.3 0 212 97 157 847.3 0 157 98 195 847.3 0 195 99 79 847.3 0 79 100 76 847.3 0 76 101 72 847.3 0 72 102 68 847.3 0 68 103 78 847.3 0 78 104 65 847.3 0 65 105 75 847.3 0 75 106 108 847.3 0 108 107 99 847.3 0 99 108 94 847.3 0 94 109 69 847.3 0 69 110 76 847.3 0 76 111 74 847.3 0 74 112 70 847.3 0 70 113 181 847.3 0 181 114 84 847.3 0 84 115 144 847.3 0 144 116 157 847.3 0 157 117 210 847.3 0 210 118 102 847.3 0 102 119 64 847.3 0 64 120 74 847.3 0 74 121 92 847.3 0 92 122 106 847.3 0 106 123 101 847.3 0 101 124 62 847.3 0 62 125 84 847.3 0 84 126 115 847.3 0 115 127 105 847.3 0 105 128 131 847.3 0 131 129 210 847.3 0 210 130 2207 847.3 0 2207 131 765 847.3 0 765 132 818 847.3 0 818 133 80 847.3 0 80 134 82 847.3 0 82 135 82 847.3 0 82 136 89 847.3 0 89 137 86 847.3 0 86 138 181 847.3 0 181 139 70 847.3 0 70 140 89 847.3 0 89 141 76 847.3 0 76 142 115 847.3 0 115 143 168 847.3 0 168 144 128 847.3 0 128 145 118 847.3 0 118 146 63 847.3 0 63 147 84 847.3 0 84 148 60 847.3 0 60 149 76 847.3 0 76 150 94 847.3 0 94 151 135 847.3 0 135 152 139 847.3 0 139 153 94 847.3 0 94 154 118 847.3 0 118 155 83 847.3 0 83 156 104 847.3 0 104 157 201 847.3 0 201 158 170 847.3 0 170 159 141 847.3 0 141 160 83 847.3 0 83 161 93 847.3 0 93 162 150 847.3 0 150 163 139 847.3 0 139 164 146 847.3 0 146 165 232 847.3 0 232 166 208 847.3 0 208 167 384 847.3 0 384 168 419 847.3 0 419 169 5224 847.3 0 5224 170 4639 847.3 0 4639 171 12764 847.3 0 12764 172 119 847.3 0 119 173 79 847.3 0 79 174 141 847.3 0 141 175 84 847.3 0 84 176 130 847.3 0 130 177 83 847.3 0 83 178 125 847.3 0 125 179 85 847.3 0 85 180 88 847.3 0 88 181 76 847.3 0 76 182 58 847.3 0 58 183 65 847.3 0 65 184 90 847.3 0 90 185 85 847.3 0 85 186 69 847.3 0 69 187 60 847.3 0 60 188 63 847.3 0 63 189 62 847.3 0 62 190 76 847.3 0 76 191 76 847.3 0 76 192 89 847.3 0 89 193 115 847.3 0 115 194 153 847.3 0 153 195 70 847.3 0 70 196 66 847.3 0 66 197 61 847.3 0 61 198 87 847.3 0 87 199 73 847.3 0 73 200 59 847.3 0 59 201 76 847.3 0 76 202 88 847.3 0 88 203 112 847.3 0 112 204 184 847.3 0 184 205 183 847.3 0 183 206 92 847.3 0 92 207 50 847.3 0 50 208 76 847.3 0 76 209 79 847.3 0 79 210 84 847.3 0 84 211 81 847.3 0 81 212 81 847.3 0 81 213 81 847.3 0 81 214 79 847.3 0 79 215 131 847.3 0 131 216 83 847.3 0 83 217 79 847.3 0 79 218 83 847.3 0 83 219 74 847.3 0 74 220 69 847.3 0 69 221 86 847.3 0 86 222 504 847.3 0 504 223 326 847.3 0 326 224 257 847.3 0 257 225 61 847.3 0 61 226 68 847.3 0 68 227 59 847.3 0 59 228 100 847.3 0 100 229 51 847.3 0 51 230 102 847.3 0 102 231 112 847.3 0 112 232 131 847.3 0 131 233 79 847.3 0 79 234 102 847.3 0 102 235 110 847.3 0 110 236 188 847.3 0 188 237 178 847.3 0 178 238 111 847.3 0 111 239 81 847.3 0 81 240 71 847.3 0 71 241 103 847.3 0 103 242 88 847.3 0 88 243 71 847.3 0 71 244 80 847.3 0 80 245 72 847.3 0 72 246 94 847.3 0 94 247 72 847.3 0 72 248 59 847.3 0 59 249 59 847.3 0 59 250 69 847.3 0 69 251 80 847.3 0 80 252 71 847.3 0 71 253 96 847.3 0 96 254 82 847.3 0 82 255 101 847.3 0 101 256 76 847.3 0 76 257 80 847.3 0 80 258 123 847.3 0 123 259 73 847.3 0 73 260 67 847.3 0 67 261 140 847.3 0 140 262 149 847.3 0 149 263 119 847.3 0 119 264 86 847.3 0 86 265 79 847.3 0 79 266 83 847.3 0 83 267 101 847.3 0 101 268 73 847.3 0 73 269 80 847.3 0 80 270 122 847.3 0 122 271 309 847.3 0 309 272 396 847.3 0 396 273 415 847.3 0 415 274 140 847.3 0 140 275 79 847.3 0 79 276 130 847.3 0 130 277 116 847.3 0 116 278 170 847.3 0 170 279 142 847.3 0 142 280 97 847.3 0 97 281 113 847.3 0 113 282 80 847.3 0 80 283 88 847.3 0 88 284 91 847.3 0 91 285 84 847.3 0 84 286 83 847.3 0 83 287 77 847.3 0 77 288 101 847.3 0 101 289 84 847.3 0 84 290 93 847.3 0 93 291 113 847.3 0 113 292 206 847.3 0 206 293 213 847.3 0 213 294 187 847.3 0 187 295 121 847.3 0 121 296 430 847.3 0 430 297 229 847.3 0 229 298 147 847.3 0 147 299 84 847.3 0 84 300 78 847.3 0 78 301 63 847.3 0 63 Finished in 4.33 s (62 us/read; 0.96 M reads/minute). === Summary === Total reads processed: 69,588 Reads with adapters: 11,535 (16.6%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 11,511 (16.5%) Total basepairs processed: 14,281,141 bp Total written (filtered): 3,184,282 bp (22.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 11535 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 3 1 16 82 0.0 3 0 82 17 11074 0.0 3 10882 181 10 1 18 34 0.0 3 2 32 19 12 0.0 3 1 2 9 20 275 0.0 3 261 1 1 12 31 1 0.0 3 0 0 0 1 42 3 0.0 3 0 0 0 3 46 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 0 3 51 1 0.0 3 0 0 0 1 62 4 0.0 3 0 0 0 4 80 8 0.0 3 0 0 0 8 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 1 193 1 0.0 3 0 0 0 1 217 18 0.0 3 0 0 1 17 255 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.65 s (56 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 11,511 Reads with adapters: 64 (0.6%) Reads written (passing filters): 11,511 (100.0%) Total basepairs processed: 3,184,282 bp Total written (filtered): 3,184,083 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 64 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 57.8% C: 26.6% G: 10.9% T: 4.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 59 179.9 0 59 4 4 45.0 0 4 6 1 2.8 0 1