This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 63.87 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 35,285 (1.4%) Reads that were too short: 10,271 (0.4%) Reads written (passing filters): 25,014 (1.0%) Total basepairs processed: 740,966,583 bp Total written (filtered): 5,895,363 bp (0.8%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 35285 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3027 601.0 0 3027 7 188 601.0 0 188 8 5511 601.0 0 5511 9 4120 601.0 0 4120 10 20 601.0 0 20 11 24 601.0 0 24 12 25 601.0 0 25 13 37 601.0 0 37 14 28 601.0 0 28 15 31 601.0 0 31 16 10 601.0 0 10 17 13 601.0 0 13 18 32 601.0 0 32 19 26 601.0 0 26 20 23 601.0 0 23 21 25 601.0 0 25 22 43 601.0 0 43 23 34 601.0 0 34 24 25 601.0 0 25 25 21 601.0 0 21 26 11 601.0 0 11 27 26 601.0 0 26 28 24 601.0 0 24 29 30 601.0 0 30 30 26 601.0 0 26 31 26 601.0 0 26 32 19 601.0 0 19 33 16 601.0 0 16 34 23 601.0 0 23 35 34 601.0 0 34 36 18 601.0 0 18 37 50 601.0 0 50 38 73 601.0 0 73 39 35 601.0 0 35 40 29 601.0 0 29 41 41 601.0 0 41 42 24 601.0 0 24 43 43 601.0 0 43 44 20 601.0 0 20 45 52 601.0 0 52 46 25 601.0 0 25 47 47 601.0 0 47 48 486 601.0 0 486 49 441 601.0 0 441 50 487 601.0 0 487 51 40 601.0 0 40 52 22 601.0 0 22 53 31 601.0 0 31 54 13 601.0 0 13 55 31 601.0 0 31 56 22 601.0 0 22 57 20 601.0 0 20 58 21 601.0 0 21 59 25 601.0 0 25 60 28 601.0 0 28 61 24 601.0 0 24 62 25 601.0 0 25 63 14 601.0 0 14 64 30 601.0 0 30 65 12 601.0 0 12 66 19 601.0 0 19 67 24 601.0 0 24 68 20 601.0 0 20 69 39 601.0 0 39 70 32 601.0 0 32 71 14 601.0 0 14 72 10 601.0 0 10 73 21 601.0 0 21 74 23 601.0 0 23 75 25 601.0 0 25 76 30 601.0 0 30 77 33 601.0 0 33 78 20 601.0 0 20 79 14 601.0 0 14 80 40 601.0 0 40 81 77 601.0 0 77 82 146 601.0 0 146 83 99 601.0 0 99 84 34 601.0 0 34 85 29 601.0 0 29 86 23 601.0 0 23 87 13 601.0 0 13 88 26 601.0 0 26 89 59 601.0 0 59 90 24 601.0 0 24 91 15 601.0 0 15 92 30 601.0 0 30 93 20 601.0 0 20 94 18 601.0 0 18 95 15 601.0 0 15 96 68 601.0 0 68 97 126 601.0 0 126 98 66 601.0 0 66 99 19 601.0 0 19 100 23 601.0 0 23 101 17 601.0 0 17 102 11 601.0 0 11 103 17 601.0 0 17 104 37 601.0 0 37 105 33 601.0 0 33 106 22 601.0 0 22 107 13 601.0 0 13 108 23 601.0 0 23 109 31 601.0 0 31 110 29 601.0 0 29 111 21 601.0 0 21 112 26 601.0 0 26 113 43 601.0 0 43 114 57 601.0 0 57 115 28 601.0 0 28 116 24 601.0 0 24 117 74 601.0 0 74 118 492 601.0 0 492 119 279 601.0 0 279 120 103 601.0 0 103 121 17 601.0 0 17 122 35 601.0 0 35 123 25 601.0 0 25 124 23 601.0 0 23 125 34 601.0 0 34 126 27 601.0 0 27 127 74 601.0 0 74 128 62 601.0 0 62 129 36 601.0 0 36 130 32 601.0 0 32 131 28 601.0 0 28 132 46 601.0 0 46 133 42 601.0 0 42 134 26 601.0 0 26 135 43 601.0 0 43 136 172 601.0 0 172 137 108 601.0 0 108 138 66 601.0 0 66 139 25 601.0 0 25 140 45 601.0 0 45 141 36 601.0 0 36 142 102 601.0 0 102 143 62 601.0 0 62 144 77 601.0 0 77 145 66 601.0 0 66 146 146 601.0 0 146 147 110 601.0 0 110 148 435 601.0 0 435 149 80 601.0 0 80 150 61 601.0 0 61 151 37 601.0 0 37 152 41 601.0 0 41 153 106 601.0 0 106 154 48 601.0 0 48 155 28 601.0 0 28 156 30 601.0 0 30 157 36 601.0 0 36 158 45 601.0 0 45 159 16 601.0 0 16 160 25 601.0 0 25 161 21 601.0 0 21 162 20 601.0 0 20 163 28 601.0 0 28 164 25 601.0 0 25 165 25 601.0 0 25 166 25 601.0 0 25 167 23 601.0 0 23 168 27 601.0 0 27 169 18 601.0 0 18 170 22 601.0 0 22 171 20 601.0 0 20 172 44 601.0 0 44 173 22 601.0 0 22 174 18 601.0 0 18 175 22 601.0 0 22 176 30 601.0 0 30 177 39 601.0 0 39 178 52 601.0 0 52 179 34 601.0 0 34 180 34 601.0 0 34 181 40 601.0 0 40 182 49 601.0 0 49 183 67 601.0 0 67 184 20 601.0 0 20 185 37 601.0 0 37 186 22 601.0 0 22 187 30 601.0 0 30 188 52 601.0 0 52 189 65 601.0 0 65 190 60 601.0 0 60 191 134 601.0 0 134 192 58 601.0 0 58 193 92 601.0 0 92 194 222 601.0 0 222 195 659 601.0 0 659 196 841 601.0 0 841 197 866 601.0 0 866 198 57 601.0 0 57 199 45 601.0 0 45 200 49 601.0 0 49 201 46 601.0 0 46 202 40 601.0 0 40 203 40 601.0 0 40 204 32 601.0 0 32 205 20 601.0 0 20 206 35 601.0 0 35 207 19 601.0 0 19 208 34 601.0 0 34 209 36 601.0 0 36 210 46 601.0 0 46 211 28 601.0 0 28 212 48 601.0 0 48 213 82 601.0 0 82 214 90 601.0 0 90 215 143 601.0 0 143 216 112 601.0 0 112 217 37 601.0 0 37 218 73 601.0 0 73 219 54 601.0 0 54 220 51 601.0 0 51 221 55 601.0 0 55 222 53 601.0 0 53 223 29 601.0 0 29 224 57 601.0 0 57 225 41 601.0 0 41 226 37 601.0 0 37 227 21 601.0 0 21 228 28 601.0 0 28 229 54 601.0 0 54 230 79 601.0 0 79 231 59 601.0 0 59 232 53 601.0 0 53 233 75 601.0 0 75 234 78 601.0 0 78 235 97 601.0 0 97 236 86 601.0 0 86 237 62 601.0 0 62 238 62 601.0 0 62 239 116 601.0 0 116 240 153 601.0 0 153 241 105 601.0 0 105 242 72 601.0 0 72 243 534 601.0 0 534 244 312 601.0 0 312 245 228 601.0 0 228 246 82 601.0 0 82 247 61 601.0 0 61 248 67 601.0 0 67 249 158 601.0 0 158 250 148 601.0 0 148 251 166 601.0 0 166 252 225 601.0 0 225 253 136 601.0 0 136 254 59 601.0 0 59 255 49 601.0 0 49 256 75 601.0 0 75 257 83 601.0 0 83 258 64 601.0 0 64 259 86 601.0 0 86 260 143 601.0 0 143 261 439 601.0 0 439 262 197 601.0 0 197 263 122 601.0 0 122 264 74 601.0 0 74 265 77 601.0 0 77 266 57 601.0 0 57 267 43 601.0 0 43 268 73 601.0 0 73 269 165 601.0 0 165 270 180 601.0 0 180 271 95 601.0 0 95 272 84 601.0 0 84 273 51 601.0 0 51 274 46 601.0 0 46 275 53 601.0 0 53 276 53 601.0 0 53 277 51 601.0 0 51 278 83 601.0 0 83 279 53 601.0 0 53 280 96 601.0 0 96 281 67 601.0 0 67 282 117 601.0 0 117 283 60 601.0 0 60 284 125 601.0 0 125 285 120 601.0 0 120 286 88 601.0 0 88 287 187 601.0 0 187 288 155 601.0 0 155 289 249 601.0 0 249 290 167 601.0 0 167 291 147 601.0 0 147 292 122 601.0 0 122 293 104 601.0 0 104 294 94 601.0 0 94 295 110 601.0 0 110 296 147 601.0 0 147 297 379 601.0 0 379 298 213 601.0 0 213 299 182 601.0 0 182 300 141 601.0 0 141 301 37 601.0 0 37 Finished in 1.34 s (54 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 25,014 Reads with adapters: 4,784 (19.1%) Reads that were too short: 13 (0.1%) Reads written (passing filters): 4,771 (19.1%) Total basepairs processed: 5,895,363 bp Total written (filtered): 1,292,564 bp (21.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4784 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 6 0.0 3 0 0 3 2 1 19 87 0.0 3 0 74 7 4 2 20 4582 0.0 4 4212 287 61 19 3 21 12 0.0 4 2 9 0 1 22 7 0.0 4 2 0 3 1 1 23 16 0.0 4 1 1 0 7 7 24 3 0.0 4 2 0 0 0 1 25 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 86 6 0.0 4 0 0 0 0 6 93 2 0.0 4 0 0 0 1 1 100 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 37 0.0 4 0 0 0 0 37 142 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 169 1 0.0 4 1 183 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 231 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.18 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 4,771 Reads with adapters: 1,049 (22.0%) Reads written (passing filters): 4,771 (100.0%) Total basepairs processed: 1,292,564 bp Total written (filtered): 1,280,998 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1049 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 10.5% G: 85.4% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 74.5 0 4 6 1 1.2 0 1 7 1 0.3 0 1 9 158 0.0 0 14 144 10 356 0.0 1 21 335 11 517 0.0 1 21 496 12 6 0.0 1 0 6 15 1 0.0 1 0 1 105 1 0.0 1 1 114 1 0.0 1 1 150 1 0.0 1 0 1 208 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.