This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.73 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 22,068 (0.9%) Reads that were too short: 3,698 (0.2%) Reads written (passing filters): 18,370 (0.7%) Total basepairs processed: 740,966,583 bp Total written (filtered): 4,762,966 bp (0.6%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 22068 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2622 601.0 0 2622 7 146 601.0 0 146 8 5296 601.0 0 5296 9 4950 601.0 0 4950 10 11 601.0 0 11 11 4 601.0 0 4 12 7 601.0 0 7 13 13 601.0 0 13 14 15 601.0 0 15 15 13 601.0 0 13 16 17 601.0 0 17 17 14 601.0 0 14 18 19 601.0 0 19 19 13 601.0 0 13 20 8 601.0 0 8 21 12 601.0 0 12 22 24 601.0 0 24 23 11 601.0 0 11 24 12 601.0 0 12 25 9 601.0 0 9 26 14 601.0 0 14 27 22 601.0 0 22 28 13 601.0 0 13 29 25 601.0 0 25 30 14 601.0 0 14 31 11 601.0 0 11 32 10 601.0 0 10 33 15 601.0 0 15 34 19 601.0 0 19 35 15 601.0 0 15 36 12 601.0 0 12 37 22 601.0 0 22 38 27 601.0 0 27 39 15 601.0 0 15 40 20 601.0 0 20 41 26 601.0 0 26 42 16 601.0 0 16 43 20 601.0 0 20 44 14 601.0 0 14 45 15 601.0 0 15 46 18 601.0 0 18 47 21 601.0 0 21 48 201 601.0 0 201 49 74 601.0 0 74 50 231 601.0 0 231 51 7 601.0 0 7 52 15 601.0 0 15 53 15 601.0 0 15 54 10 601.0 0 10 55 17 601.0 0 17 56 16 601.0 0 16 57 12 601.0 0 12 58 10 601.0 0 10 59 16 601.0 0 16 60 11 601.0 0 11 61 14 601.0 0 14 62 16 601.0 0 16 63 7 601.0 0 7 64 14 601.0 0 14 65 14 601.0 0 14 66 6 601.0 0 6 67 18 601.0 0 18 68 11 601.0 0 11 69 26 601.0 0 26 70 24 601.0 0 24 71 10 601.0 0 10 72 6 601.0 0 6 73 19 601.0 0 19 74 13 601.0 0 13 75 11 601.0 0 11 76 11 601.0 0 11 77 10 601.0 0 10 78 3 601.0 0 3 79 9 601.0 0 9 80 22 601.0 0 22 81 16 601.0 0 16 82 34 601.0 0 34 83 32 601.0 0 32 84 20 601.0 0 20 85 39 601.0 0 39 86 14 601.0 0 14 87 18 601.0 0 18 88 14 601.0 0 14 89 13 601.0 0 13 90 17 601.0 0 17 91 9 601.0 0 9 92 23 601.0 0 23 93 16 601.0 0 16 94 19 601.0 0 19 95 16 601.0 0 16 96 30 601.0 0 30 97 37 601.0 0 37 98 28 601.0 0 28 99 20 601.0 0 20 100 13 601.0 0 13 101 8 601.0 0 8 102 12 601.0 0 12 103 9 601.0 0 9 104 18 601.0 0 18 105 19 601.0 0 19 106 12 601.0 0 12 107 17 601.0 0 17 108 14 601.0 0 14 109 23 601.0 0 23 110 13 601.0 0 13 111 9 601.0 0 9 112 9 601.0 0 9 113 11 601.0 0 11 114 26 601.0 0 26 115 32 601.0 0 32 116 20 601.0 0 20 117 30 601.0 0 30 118 124 601.0 0 124 119 69 601.0 0 69 120 28 601.0 0 28 121 13 601.0 0 13 122 32 601.0 0 32 123 16 601.0 0 16 124 18 601.0 0 18 125 13 601.0 0 13 126 17 601.0 0 17 127 31 601.0 0 31 128 48 601.0 0 48 129 11 601.0 0 11 130 19 601.0 0 19 131 7 601.0 0 7 132 24 601.0 0 24 133 17 601.0 0 17 134 15 601.0 0 15 135 17 601.0 0 17 136 41 601.0 0 41 137 30 601.0 0 30 138 31 601.0 0 31 139 17 601.0 0 17 140 22 601.0 0 22 141 18 601.0 0 18 142 31 601.0 0 31 143 22 601.0 0 22 144 21 601.0 0 21 145 24 601.0 0 24 146 104 601.0 0 104 147 63 601.0 0 63 148 174 601.0 0 174 149 32 601.0 0 32 150 17 601.0 0 17 151 31 601.0 0 31 152 35 601.0 0 35 153 119 601.0 0 119 154 17 601.0 0 17 155 14 601.0 0 14 156 26 601.0 0 26 157 13 601.0 0 13 158 18 601.0 0 18 159 10 601.0 0 10 160 14 601.0 0 14 161 14 601.0 0 14 162 17 601.0 0 17 163 19 601.0 0 19 164 15 601.0 0 15 165 16 601.0 0 16 166 11 601.0 0 11 167 22 601.0 0 22 168 15 601.0 0 15 169 14 601.0 0 14 170 19 601.0 0 19 171 19 601.0 0 19 172 13 601.0 0 13 173 15 601.0 0 15 174 15 601.0 0 15 175 16 601.0 0 16 176 16 601.0 0 16 177 21 601.0 0 21 178 20 601.0 0 20 179 19 601.0 0 19 180 21 601.0 0 21 181 24 601.0 0 24 182 36 601.0 0 36 183 64 601.0 0 64 184 13 601.0 0 13 185 10 601.0 0 10 186 7 601.0 0 7 187 16 601.0 0 16 188 19 601.0 0 19 189 30 601.0 0 30 190 17 601.0 0 17 191 98 601.0 0 98 192 41 601.0 0 41 193 41 601.0 0 41 194 39 601.0 0 39 195 231 601.0 0 231 196 308 601.0 0 308 197 302 601.0 0 302 198 21 601.0 0 21 199 13 601.0 0 13 200 15 601.0 0 15 201 16 601.0 0 16 202 11 601.0 0 11 203 17 601.0 0 17 204 14 601.0 0 14 205 10 601.0 0 10 206 9 601.0 0 9 207 13 601.0 0 13 208 15 601.0 0 15 209 23 601.0 0 23 210 20 601.0 0 20 211 18 601.0 0 18 212 9 601.0 0 9 213 15 601.0 0 15 214 15 601.0 0 15 215 36 601.0 0 36 216 37 601.0 0 37 217 12 601.0 0 12 218 58 601.0 0 58 219 20 601.0 0 20 220 11 601.0 0 11 221 28 601.0 0 28 222 11 601.0 0 11 223 11 601.0 0 11 224 15 601.0 0 15 225 21 601.0 0 21 226 19 601.0 0 19 227 13 601.0 0 13 228 18 601.0 0 18 229 20 601.0 0 20 230 22 601.0 0 22 231 19 601.0 0 19 232 21 601.0 0 21 233 18 601.0 0 18 234 29 601.0 0 29 235 37 601.0 0 37 236 19 601.0 0 19 237 21 601.0 0 21 238 24 601.0 0 24 239 29 601.0 0 29 240 35 601.0 0 35 241 33 601.0 0 33 242 16 601.0 0 16 243 121 601.0 0 121 244 78 601.0 0 78 245 62 601.0 0 62 246 32 601.0 0 32 247 17 601.0 0 17 248 28 601.0 0 28 249 38 601.0 0 38 250 56 601.0 0 56 251 193 601.0 0 193 252 30 601.0 0 30 253 19 601.0 0 19 254 22 601.0 0 22 255 19 601.0 0 19 256 22 601.0 0 22 257 39 601.0 0 39 258 22 601.0 0 22 259 41 601.0 0 41 260 49 601.0 0 49 261 111 601.0 0 111 262 52 601.0 0 52 263 38 601.0 0 38 264 14 601.0 0 14 265 15 601.0 0 15 266 20 601.0 0 20 267 16 601.0 0 16 268 14 601.0 0 14 269 44 601.0 0 44 270 37 601.0 0 37 271 50 601.0 0 50 272 25 601.0 0 25 273 18 601.0 0 18 274 16 601.0 0 16 275 29 601.0 0 29 276 19 601.0 0 19 277 19 601.0 0 19 278 25 601.0 0 25 279 30 601.0 0 30 280 23 601.0 0 23 281 27 601.0 0 27 282 35 601.0 0 35 283 28 601.0 0 28 284 44 601.0 0 44 285 65 601.0 0 65 286 45 601.0 0 45 287 230 601.0 0 230 288 108 601.0 0 108 289 146 601.0 0 146 290 58 601.0 0 58 291 42 601.0 0 42 292 43 601.0 0 43 293 41 601.0 0 41 294 36 601.0 0 36 295 34 601.0 0 34 296 47 601.0 0 47 297 102 601.0 0 102 298 74 601.0 0 74 299 66 601.0 0 66 300 48 601.0 0 48 301 34 601.0 0 34 Finished in 0.95 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 18,370 Reads with adapters: 7,628 (41.5%) Reads that were too short: 15 (0.1%) Reads written (passing filters): 7,613 (41.4%) Total basepairs processed: 4,762,966 bp Total written (filtered): 2,093,091 bp (43.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7628 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 1 0.0 3 0 0 1 16 37 0.0 3 0 36 1 17 5049 0.0 3 4966 81 2 18 14 0.0 3 0 12 2 19 26 0.0 3 11 11 4 20 2438 0.0 3 2363 56 3 16 21 5 0.0 3 0 5 22 1 0.0 3 0 0 0 1 23 2 0.0 3 0 0 0 2 29 7 0.0 3 0 0 1 6 31 3 0.0 3 0 0 0 3 32 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 53 4 0.0 3 0 0 0 4 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 63 1 0.0 3 1 64 2 0.0 3 0 0 0 2 74 6 0.0 3 0 0 0 6 91 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 1 137 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 8 0.0 3 0 0 0 8 146 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.28 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 7,613 Reads with adapters: 117 (1.5%) Reads written (passing filters): 7,613 (100.0%) Total basepairs processed: 2,093,091 bp Total written (filtered): 2,092,697 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 117 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 53.8% C: 6.8% G: 37.6% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 75 119.0 0 75 4 41 29.7 0 41 5 1 7.4 0 1