This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 63.33 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 68,696 (2.8%) Reads that were too short: 27,815 (1.1%) Reads written (passing filters): 40,881 (1.7%) Total basepairs processed: 740,966,583 bp Total written (filtered): 8,188,498 bp (1.1%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 68696 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 312 601.0 0 312 7 1876 601.0 0 1876 8 1651 601.0 0 1651 9 84 601.0 0 84 10 124 601.0 0 124 11 69 601.0 0 69 12 56 601.0 0 56 13 69 601.0 0 69 14 73 601.0 0 73 15 65 601.0 0 65 16 50 601.0 0 50 17 79 601.0 0 79 18 92 601.0 0 92 19 240 601.0 0 240 20 61 601.0 0 61 21 48 601.0 0 48 22 43 601.0 0 43 23 47 601.0 0 47 24 46 601.0 0 46 25 53 601.0 0 53 26 65 601.0 0 65 27 55 601.0 0 55 28 70 601.0 0 70 29 76 601.0 0 76 30 60 601.0 0 60 31 44 601.0 0 44 32 48 601.0 0 48 33 52 601.0 0 52 34 57 601.0 0 57 35 61 601.0 0 61 36 69 601.0 0 69 37 55 601.0 0 55 38 49 601.0 0 49 39 73 601.0 0 73 40 70 601.0 0 70 41 59 601.0 0 59 42 84 601.0 0 84 43 83 601.0 0 83 44 57 601.0 0 57 45 40 601.0 0 40 46 102 601.0 0 102 47 110 601.0 0 110 48 82 601.0 0 82 49 120 601.0 0 120 50 84 601.0 0 84 51 57 601.0 0 57 52 90 601.0 0 90 53 143 601.0 0 143 54 109 601.0 0 109 55 91 601.0 0 91 56 82 601.0 0 82 57 93 601.0 0 93 58 87 601.0 0 87 59 84 601.0 0 84 60 168 601.0 0 168 61 137 601.0 0 137 62 211 601.0 0 211 63 118 601.0 0 118 64 100 601.0 0 100 65 83 601.0 0 83 66 81 601.0 0 81 67 101 601.0 0 101 68 83 601.0 0 83 69 66 601.0 0 66 70 102 601.0 0 102 71 143 601.0 0 143 72 176 601.0 0 176 73 1204 601.0 0 1204 74 1080 601.0 0 1080 75 661 601.0 0 661 76 264 601.0 0 264 77 5454 601.0 0 5454 78 2228 601.0 0 2228 79 1183 601.0 0 1183 80 56 601.0 0 56 81 75 601.0 0 75 82 66 601.0 0 66 83 43 601.0 0 43 84 62 601.0 0 62 85 58 601.0 0 58 86 71 601.0 0 71 87 46 601.0 0 46 88 80 601.0 0 80 89 69 601.0 0 69 90 78 601.0 0 78 91 61 601.0 0 61 92 94 601.0 0 94 93 74 601.0 0 74 94 64 601.0 0 64 95 72 601.0 0 72 96 72 601.0 0 72 97 64 601.0 0 64 98 69 601.0 0 69 99 107 601.0 0 107 100 71 601.0 0 71 101 70 601.0 0 70 102 46 601.0 0 46 103 51 601.0 0 51 104 80 601.0 0 80 105 75 601.0 0 75 106 117 601.0 0 117 107 266 601.0 0 266 108 281 601.0 0 281 109 72 601.0 0 72 110 68 601.0 0 68 111 46 601.0 0 46 112 130 601.0 0 130 113 70 601.0 0 70 114 76 601.0 0 76 115 95 601.0 0 95 116 91 601.0 0 91 117 82 601.0 0 82 118 65 601.0 0 65 119 66 601.0 0 66 120 81 601.0 0 81 121 74 601.0 0 74 122 72 601.0 0 72 123 59 601.0 0 59 124 90 601.0 0 90 125 90 601.0 0 90 126 80 601.0 0 80 127 120 601.0 0 120 128 524 601.0 0 524 129 408 601.0 0 408 130 264 601.0 0 264 131 106 601.0 0 106 132 87 601.0 0 87 133 140 601.0 0 140 134 228 601.0 0 228 135 263 601.0 0 263 136 518 601.0 0 518 137 164 601.0 0 164 138 125 601.0 0 125 139 120 601.0 0 120 140 109 601.0 0 109 141 80 601.0 0 80 142 132 601.0 0 132 143 102 601.0 0 102 144 75 601.0 0 75 145 172 601.0 0 172 146 2103 601.0 0 2103 147 926 601.0 0 926 148 348 601.0 0 348 149 259 601.0 0 259 150 138 601.0 0 138 151 75 601.0 0 75 152 75 601.0 0 75 153 77 601.0 0 77 154 68 601.0 0 68 155 145 601.0 0 145 156 173 601.0 0 173 157 199 601.0 0 199 158 107 601.0 0 107 159 232 601.0 0 232 160 205 601.0 0 205 161 178 601.0 0 178 162 86 601.0 0 86 163 73 601.0 0 73 164 381 601.0 0 381 165 235 601.0 0 235 166 178 601.0 0 178 167 110 601.0 0 110 168 100 601.0 0 100 169 70 601.0 0 70 170 105 601.0 0 105 171 80 601.0 0 80 172 58 601.0 0 58 173 65 601.0 0 65 174 64 601.0 0 64 175 72 601.0 0 72 176 52 601.0 0 52 177 72 601.0 0 72 178 62 601.0 0 62 179 102 601.0 0 102 180 93 601.0 0 93 181 105 601.0 0 105 182 75 601.0 0 75 183 75 601.0 0 75 184 398 601.0 0 398 185 403 601.0 0 403 186 410 601.0 0 410 187 144 601.0 0 144 188 115 601.0 0 115 189 61 601.0 0 61 190 55 601.0 0 55 191 98 601.0 0 98 192 107 601.0 0 107 193 110 601.0 0 110 194 108 601.0 0 108 195 828 601.0 0 828 196 641 601.0 0 641 197 308 601.0 0 308 198 133 601.0 0 133 199 140 601.0 0 140 200 120 601.0 0 120 201 157 601.0 0 157 202 77 601.0 0 77 203 101 601.0 0 101 204 75 601.0 0 75 205 118 601.0 0 118 206 173 601.0 0 173 207 75 601.0 0 75 208 95 601.0 0 95 209 78 601.0 0 78 210 88 601.0 0 88 211 108 601.0 0 108 212 126 601.0 0 126 213 128 601.0 0 128 214 132 601.0 0 132 215 213 601.0 0 213 216 283 601.0 0 283 217 301 601.0 0 301 218 130 601.0 0 130 219 185 601.0 0 185 220 124 601.0 0 124 221 103 601.0 0 103 222 64 601.0 0 64 223 103 601.0 0 103 224 85 601.0 0 85 225 195 601.0 0 195 226 192 601.0 0 192 227 130 601.0 0 130 228 81 601.0 0 81 229 82 601.0 0 82 230 120 601.0 0 120 231 93 601.0 0 93 232 91 601.0 0 91 233 111 601.0 0 111 234 167 601.0 0 167 235 253 601.0 0 253 236 1107 601.0 0 1107 237 1690 601.0 0 1690 238 2610 601.0 0 2610 239 205 601.0 0 205 240 132 601.0 0 132 241 162 601.0 0 162 242 98 601.0 0 98 243 85 601.0 0 85 244 127 601.0 0 127 245 195 601.0 0 195 246 436 601.0 0 436 247 593 601.0 0 593 248 446 601.0 0 446 249 218 601.0 0 218 250 398 601.0 0 398 251 496 601.0 0 496 252 589 601.0 0 589 253 573 601.0 0 573 254 235 601.0 0 235 255 155 601.0 0 155 256 106 601.0 0 106 257 102 601.0 0 102 258 112 601.0 0 112 259 142 601.0 0 142 260 80 601.0 0 80 261 112 601.0 0 112 262 102 601.0 0 102 263 159 601.0 0 159 264 141 601.0 0 141 265 93 601.0 0 93 266 96 601.0 0 96 267 96 601.0 0 96 268 120 601.0 0 120 269 81 601.0 0 81 270 83 601.0 0 83 271 83 601.0 0 83 272 139 601.0 0 139 273 150 601.0 0 150 274 134 601.0 0 134 275 94 601.0 0 94 276 164 601.0 0 164 277 163 601.0 0 163 278 235 601.0 0 235 279 239 601.0 0 239 280 293 601.0 0 293 281 624 601.0 0 624 282 423 601.0 0 423 283 289 601.0 0 289 284 752 601.0 0 752 285 492 601.0 0 492 286 291 601.0 0 291 287 144 601.0 0 144 288 158 601.0 0 158 289 186 601.0 0 186 290 130 601.0 0 130 291 124 601.0 0 124 292 169 601.0 0 169 293 319 601.0 0 319 294 317 601.0 0 317 295 335 601.0 0 335 296 782 601.0 0 782 297 979 601.0 0 979 298 1238 601.0 0 1238 299 667 601.0 0 667 300 442 601.0 0 442 301 605 601.0 0 605 Finished in 1.91 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 40,881 Reads with adapters: 1,888 (4.6%) Reads that were too short: 35 (0.1%) Reads written (passing filters): 1,853 (4.5%) Total basepairs processed: 8,188,498 bp Total written (filtered): 493,864 bp (6.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1888 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 26 0.0 3 0 21 1 3 1 20 1583 0.0 4 1417 128 29 7 2 21 7 0.0 4 0 4 1 0 2 22 8 0.0 4 2 3 2 0 1 23 120 0.0 4 107 4 2 4 3 26 2 0.0 4 0 0 0 1 1 27 2 0.0 4 0 0 0 0 2 31 1 0.0 4 0 0 0 0 1 57 40 0.0 4 0 0 0 0 40 60 1 0.0 4 0 0 0 0 1 61 7 0.0 4 0 0 0 0 7 67 1 0.0 4 0 0 0 0 1 83 9 0.0 4 0 0 0 0 9 100 3 0.0 4 0 0 0 0 3 101 2 0.0 4 0 0 0 1 1 103 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 1 107 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 0 2 109 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 47 0.0 4 0 0 0 0 47 137 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 141 1 0.0 4 1 142 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 165 2 0.0 4 0 0 0 0 2 169 2 0.0 4 0 0 0 0 2 186 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 241 2 0.0 4 0 0 0 0 2 289 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.05 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 1,853 Reads with adapters: 371 (20.0%) Reads written (passing filters): 1,853 (100.0%) Total basepairs processed: 493,864 bp Total written (filtered): 489,705 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 371 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 11.1% G: 85.2% T: 2.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 29.0 0 1 4 1 7.2 0 1 9 2 0.0 0 0 2 10 102 0.0 1 12 90 11 263 0.0 1 16 247 12 1 0.0 1 0 1 209 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.