This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.34 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 44,570 (1.8%) Reads that were too short: 17,067 (0.7%) Reads written (passing filters): 27,503 (1.1%) Total basepairs processed: 740,966,583 bp Total written (filtered): 5,615,023 bp (0.8%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 44570 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 322 601.0 0 322 7 1726 601.0 0 1726 8 2021 601.0 0 2021 9 69 601.0 0 69 10 89 601.0 0 89 11 53 601.0 0 53 12 43 601.0 0 43 13 48 601.0 0 48 14 44 601.0 0 44 15 52 601.0 0 52 16 59 601.0 0 59 17 50 601.0 0 50 18 63 601.0 0 63 19 69 601.0 0 69 20 53 601.0 0 53 21 44 601.0 0 44 22 38 601.0 0 38 23 49 601.0 0 49 24 53 601.0 0 53 25 54 601.0 0 54 26 45 601.0 0 45 27 47 601.0 0 47 28 53 601.0 0 53 29 73 601.0 0 73 30 65 601.0 0 65 31 51 601.0 0 51 32 57 601.0 0 57 33 75 601.0 0 75 34 48 601.0 0 48 35 55 601.0 0 55 36 66 601.0 0 66 37 58 601.0 0 58 38 58 601.0 0 58 39 68 601.0 0 68 40 71 601.0 0 71 41 48 601.0 0 48 42 80 601.0 0 80 43 78 601.0 0 78 44 60 601.0 0 60 45 43 601.0 0 43 46 105 601.0 0 105 47 87 601.0 0 87 48 47 601.0 0 47 49 82 601.0 0 82 50 61 601.0 0 61 51 39 601.0 0 39 52 71 601.0 0 71 53 101 601.0 0 101 54 64 601.0 0 64 55 94 601.0 0 94 56 76 601.0 0 76 57 106 601.0 0 106 58 64 601.0 0 64 59 67 601.0 0 67 60 105 601.0 0 105 61 94 601.0 0 94 62 91 601.0 0 91 63 76 601.0 0 76 64 231 601.0 0 231 65 92 601.0 0 92 66 65 601.0 0 65 67 99 601.0 0 99 68 62 601.0 0 62 69 62 601.0 0 62 70 91 601.0 0 91 71 100 601.0 0 100 72 82 601.0 0 82 73 672 601.0 0 672 74 589 601.0 0 589 75 303 601.0 0 303 76 138 601.0 0 138 77 1539 601.0 0 1539 78 631 601.0 0 631 79 375 601.0 0 375 80 67 601.0 0 67 81 60 601.0 0 60 82 62 601.0 0 62 83 45 601.0 0 45 84 56 601.0 0 56 85 69 601.0 0 69 86 52 601.0 0 52 87 51 601.0 0 51 88 64 601.0 0 64 89 78 601.0 0 78 90 49 601.0 0 49 91 58 601.0 0 58 92 81 601.0 0 81 93 67 601.0 0 67 94 66 601.0 0 66 95 64 601.0 0 64 96 74 601.0 0 74 97 71 601.0 0 71 98 69 601.0 0 69 99 71 601.0 0 71 100 77 601.0 0 77 101 84 601.0 0 84 102 36 601.0 0 36 103 85 601.0 0 85 104 70 601.0 0 70 105 81 601.0 0 81 106 115 601.0 0 115 107 313 601.0 0 313 108 341 601.0 0 341 109 82 601.0 0 82 110 63 601.0 0 63 111 48 601.0 0 48 112 120 601.0 0 120 113 81 601.0 0 81 114 69 601.0 0 69 115 79 601.0 0 79 116 76 601.0 0 76 117 66 601.0 0 66 118 81 601.0 0 81 119 56 601.0 0 56 120 73 601.0 0 73 121 75 601.0 0 75 122 52 601.0 0 52 123 64 601.0 0 64 124 66 601.0 0 66 125 87 601.0 0 87 126 76 601.0 0 76 127 108 601.0 0 108 128 520 601.0 0 520 129 304 601.0 0 304 130 243 601.0 0 243 131 90 601.0 0 90 132 78 601.0 0 78 133 124 601.0 0 124 134 175 601.0 0 175 135 211 601.0 0 211 136 434 601.0 0 434 137 147 601.0 0 147 138 72 601.0 0 72 139 112 601.0 0 112 140 91 601.0 0 91 141 63 601.0 0 63 142 142 601.0 0 142 143 92 601.0 0 92 144 76 601.0 0 76 145 132 601.0 0 132 146 1718 601.0 0 1718 147 695 601.0 0 695 148 271 601.0 0 271 149 131 601.0 0 131 150 96 601.0 0 96 151 62 601.0 0 62 152 60 601.0 0 60 153 79 601.0 0 79 154 60 601.0 0 60 155 100 601.0 0 100 156 156 601.0 0 156 157 178 601.0 0 178 158 64 601.0 0 64 159 96 601.0 0 96 160 88 601.0 0 88 161 76 601.0 0 76 162 71 601.0 0 71 163 56 601.0 0 56 164 206 601.0 0 206 165 136 601.0 0 136 166 107 601.0 0 107 167 71 601.0 0 71 168 51 601.0 0 51 169 75 601.0 0 75 170 66 601.0 0 66 171 78 601.0 0 78 172 55 601.0 0 55 173 54 601.0 0 54 174 63 601.0 0 63 175 58 601.0 0 58 176 41 601.0 0 41 177 61 601.0 0 61 178 44 601.0 0 44 179 51 601.0 0 51 180 49 601.0 0 49 181 44 601.0 0 44 182 65 601.0 0 65 183 49 601.0 0 49 184 169 601.0 0 169 185 147 601.0 0 147 186 147 601.0 0 147 187 72 601.0 0 72 188 53 601.0 0 53 189 40 601.0 0 40 190 45 601.0 0 45 191 61 601.0 0 61 192 64 601.0 0 64 193 76 601.0 0 76 194 61 601.0 0 61 195 509 601.0 0 509 196 379 601.0 0 379 197 131 601.0 0 131 198 73 601.0 0 73 199 78 601.0 0 78 200 70 601.0 0 70 201 84 601.0 0 84 202 55 601.0 0 55 203 67 601.0 0 67 204 65 601.0 0 65 205 87 601.0 0 87 206 89 601.0 0 89 207 56 601.0 0 56 208 46 601.0 0 46 209 68 601.0 0 68 210 54 601.0 0 54 211 52 601.0 0 52 212 47 601.0 0 47 213 47 601.0 0 47 214 48 601.0 0 48 215 112 601.0 0 112 216 154 601.0 0 154 217 150 601.0 0 150 218 87 601.0 0 87 219 97 601.0 0 97 220 79 601.0 0 79 221 47 601.0 0 47 222 59 601.0 0 59 223 55 601.0 0 55 224 61 601.0 0 61 225 105 601.0 0 105 226 69 601.0 0 69 227 59 601.0 0 59 228 59 601.0 0 59 229 67 601.0 0 67 230 48 601.0 0 48 231 87 601.0 0 87 232 83 601.0 0 83 233 83 601.0 0 83 234 123 601.0 0 123 235 191 601.0 0 191 236 1375 601.0 0 1375 237 2192 601.0 0 2192 238 3556 601.0 0 3556 239 89 601.0 0 89 240 67 601.0 0 67 241 66 601.0 0 66 242 52 601.0 0 52 243 55 601.0 0 55 244 60 601.0 0 60 245 71 601.0 0 71 246 206 601.0 0 206 247 233 601.0 0 233 248 244 601.0 0 244 249 95 601.0 0 95 250 234 601.0 0 234 251 118 601.0 0 118 252 160 601.0 0 160 253 128 601.0 0 128 254 81 601.0 0 81 255 51 601.0 0 51 256 51 601.0 0 51 257 59 601.0 0 59 258 41 601.0 0 41 259 112 601.0 0 112 260 36 601.0 0 36 261 48 601.0 0 48 262 48 601.0 0 48 263 74 601.0 0 74 264 53 601.0 0 53 265 45 601.0 0 45 266 56 601.0 0 56 267 42 601.0 0 42 268 61 601.0 0 61 269 64 601.0 0 64 270 70 601.0 0 70 271 52 601.0 0 52 272 58 601.0 0 58 273 87 601.0 0 87 274 71 601.0 0 71 275 47 601.0 0 47 276 113 601.0 0 113 277 93 601.0 0 93 278 128 601.0 0 128 279 81 601.0 0 81 280 150 601.0 0 150 281 338 601.0 0 338 282 244 601.0 0 244 283 130 601.0 0 130 284 189 601.0 0 189 285 129 601.0 0 129 286 74 601.0 0 74 287 79 601.0 0 79 288 61 601.0 0 61 289 70 601.0 0 70 290 71 601.0 0 71 291 78 601.0 0 78 292 83 601.0 0 83 293 85 601.0 0 85 294 109 601.0 0 109 295 114 601.0 0 114 296 263 601.0 0 263 297 340 601.0 0 340 298 410 601.0 0 410 299 261 601.0 0 261 300 202 601.0 0 202 301 208 601.0 0 208 Finished in 1.20 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 27,503 Reads with adapters: 1,856 (6.7%) Reads that were too short: 41 (0.1%) Reads written (passing filters): 1,815 (6.6%) Total basepairs processed: 5,615,023 bp Total written (filtered): 481,797 bp (8.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1856 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 12 0.0 3 0 12 17 1651 0.0 3 1614 36 1 18 6 0.0 3 1 5 19 3 0.0 3 0 0 3 20 3 0.0 3 0 0 0 3 33 2 0.0 3 0 0 0 2 39 48 0.0 3 0 0 0 48 44 1 0.0 3 0 0 0 1 45 15 0.0 3 0 0 0 15 46 4 0.0 3 0 0 0 4 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 88 7 0.0 3 0 0 0 7 92 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 114 43 0.0 3 0 0 0 43 115 9 0.0 3 0 0 0 9 116 1 0.0 3 0 0 0 1 117 6 0.0 3 0 0 0 6 118 9 0.0 3 0 0 0 9 119 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 1 1 220 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 237 1 0.0 3 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 1,815 Reads with adapters: 4 (0.2%) Reads written (passing filters): 1,815 (100.0%) Total basepairs processed: 481,797 bp Total written (filtered): 481,784 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 4 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.0% C: 0.0% G: 50.0% T: 25.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 28.4 0 3 4 1 7.1 0 1