This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 63.41 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 67,160 (2.7%) Reads that were too short: 31,551 (1.3%) Reads written (passing filters): 35,609 (1.4%) Total basepairs processed: 740,966,583 bp Total written (filtered): 8,798,623 bp (1.2%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 67160 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 6702 601.0 0 6702 7 7506 601.0 0 7506 8 136 601.0 0 136 9 7834 601.0 0 7834 10 58 601.0 0 58 11 10 601.0 0 10 12 87 601.0 0 87 13 16 601.0 0 16 14 17 601.0 0 17 15 13 601.0 0 13 16 14 601.0 0 14 17 11 601.0 0 11 18 17 601.0 0 17 19 21 601.0 0 21 20 16 601.0 0 16 21 14 601.0 0 14 22 11 601.0 0 11 23 16 601.0 0 16 24 10 601.0 0 10 25 11 601.0 0 11 26 15 601.0 0 15 27 12 601.0 0 12 28 15 601.0 0 15 29 18 601.0 0 18 30 19 601.0 0 19 31 22 601.0 0 22 32 14 601.0 0 14 33 20 601.0 0 20 34 31 601.0 0 31 35 18 601.0 0 18 36 25 601.0 0 25 37 22 601.0 0 22 38 24 601.0 0 24 39 26 601.0 0 26 40 17 601.0 0 17 41 18 601.0 0 18 42 29 601.0 0 29 43 21 601.0 0 21 44 19 601.0 0 19 45 19 601.0 0 19 46 22 601.0 0 22 47 16 601.0 0 16 48 18 601.0 0 18 49 20 601.0 0 20 50 19 601.0 0 19 51 12 601.0 0 12 52 9 601.0 0 9 53 23 601.0 0 23 54 14 601.0 0 14 55 200 601.0 0 200 56 52 601.0 0 52 57 23 601.0 0 23 58 8 601.0 0 8 59 21 601.0 0 21 60 15 601.0 0 15 61 21 601.0 0 21 62 10 601.0 0 10 63 19 601.0 0 19 64 20 601.0 0 20 65 17 601.0 0 17 66 25 601.0 0 25 67 27 601.0 0 27 68 20 601.0 0 20 69 18 601.0 0 18 70 44 601.0 0 44 71 33 601.0 0 33 72 80 601.0 0 80 73 16 601.0 0 16 74 26 601.0 0 26 75 16 601.0 0 16 76 13 601.0 0 13 77 33 601.0 0 33 78 29 601.0 0 29 79 35 601.0 0 35 80 49 601.0 0 49 81 168 601.0 0 168 82 236 601.0 0 236 83 323 601.0 0 323 84 23 601.0 0 23 85 19 601.0 0 19 86 17 601.0 0 17 87 35 601.0 0 35 88 19 601.0 0 19 89 17 601.0 0 17 90 22 601.0 0 22 91 24 601.0 0 24 92 17 601.0 0 17 93 21 601.0 0 21 94 30 601.0 0 30 95 27 601.0 0 27 96 23 601.0 0 23 97 35 601.0 0 35 98 32 601.0 0 32 99 29 601.0 0 29 100 31 601.0 0 31 101 146 601.0 0 146 102 288 601.0 0 288 103 139 601.0 0 139 104 85 601.0 0 85 105 37 601.0 0 37 106 102 601.0 0 102 107 69 601.0 0 69 108 338 601.0 0 338 109 71 601.0 0 71 110 54 601.0 0 54 111 80 601.0 0 80 112 126 601.0 0 126 113 33 601.0 0 33 114 38 601.0 0 38 115 50 601.0 0 50 116 41 601.0 0 41 117 35 601.0 0 35 118 106 601.0 0 106 119 43 601.0 0 43 120 45 601.0 0 45 121 46 601.0 0 46 122 68 601.0 0 68 123 65 601.0 0 65 124 84 601.0 0 84 125 61 601.0 0 61 126 75 601.0 0 75 127 99 601.0 0 99 128 30 601.0 0 30 129 56 601.0 0 56 130 38 601.0 0 38 131 98 601.0 0 98 132 117 601.0 0 117 133 527 601.0 0 527 134 197 601.0 0 197 135 106 601.0 0 106 136 128 601.0 0 128 137 87 601.0 0 87 138 172 601.0 0 172 139 126 601.0 0 126 140 157 601.0 0 157 141 97 601.0 0 97 142 88 601.0 0 88 143 36 601.0 0 36 144 61 601.0 0 61 145 61 601.0 0 61 146 179 601.0 0 179 147 162 601.0 0 162 148 377 601.0 0 377 149 52 601.0 0 52 150 38 601.0 0 38 151 54 601.0 0 54 152 40 601.0 0 40 153 72 601.0 0 72 154 75 601.0 0 75 155 97 601.0 0 97 156 193 601.0 0 193 157 129 601.0 0 129 158 233 601.0 0 233 159 85 601.0 0 85 160 76 601.0 0 76 161 77 601.0 0 77 162 65 601.0 0 65 163 55 601.0 0 55 164 64 601.0 0 64 165 85 601.0 0 85 166 92 601.0 0 92 167 792 601.0 0 792 168 65 601.0 0 65 169 196 601.0 0 196 170 37 601.0 0 37 171 62 601.0 0 62 172 71 601.0 0 71 173 73 601.0 0 73 174 54 601.0 0 54 175 44 601.0 0 44 176 42 601.0 0 42 177 40 601.0 0 40 178 44 601.0 0 44 179 72 601.0 0 72 180 258 601.0 0 258 181 312 601.0 0 312 182 248 601.0 0 248 183 62 601.0 0 62 184 66 601.0 0 66 185 66 601.0 0 66 186 48 601.0 0 48 187 48 601.0 0 48 188 51 601.0 0 51 189 57 601.0 0 57 190 70 601.0 0 70 191 33 601.0 0 33 192 42 601.0 0 42 193 47 601.0 0 47 194 57 601.0 0 57 195 62 601.0 0 62 196 48 601.0 0 48 197 46 601.0 0 46 198 83 601.0 0 83 199 87 601.0 0 87 200 146 601.0 0 146 201 79 601.0 0 79 202 35 601.0 0 35 203 57 601.0 0 57 204 70 601.0 0 70 205 95 601.0 0 95 206 131 601.0 0 131 207 144 601.0 0 144 208 159 601.0 0 159 209 220 601.0 0 220 210 245 601.0 0 245 211 1322 601.0 0 1322 212 3912 601.0 0 3912 213 9229 601.0 0 9229 214 2885 601.0 0 2885 215 206 601.0 0 206 216 48 601.0 0 48 217 47 601.0 0 47 218 81 601.0 0 81 219 101 601.0 0 101 220 76 601.0 0 76 221 74 601.0 0 74 222 53 601.0 0 53 223 196 601.0 0 196 224 450 601.0 0 450 225 363 601.0 0 363 226 215 601.0 0 215 227 53 601.0 0 53 228 63 601.0 0 63 229 68 601.0 0 68 230 82 601.0 0 82 231 40 601.0 0 40 232 41 601.0 0 41 233 62 601.0 0 62 234 59 601.0 0 59 235 50 601.0 0 50 236 43 601.0 0 43 237 42 601.0 0 42 238 70 601.0 0 70 239 75 601.0 0 75 240 69 601.0 0 69 241 84 601.0 0 84 242 70 601.0 0 70 243 74 601.0 0 74 244 445 601.0 0 445 245 77 601.0 0 77 246 100 601.0 0 100 247 58 601.0 0 58 248 46 601.0 0 46 249 44 601.0 0 44 250 35 601.0 0 35 251 58 601.0 0 58 252 80 601.0 0 80 253 77 601.0 0 77 254 89 601.0 0 89 255 107 601.0 0 107 256 142 601.0 0 142 257 142 601.0 0 142 258 146 601.0 0 146 259 84 601.0 0 84 260 95 601.0 0 95 261 76 601.0 0 76 262 71 601.0 0 71 263 68 601.0 0 68 264 74 601.0 0 74 265 76 601.0 0 76 266 143 601.0 0 143 267 191 601.0 0 191 268 198 601.0 0 198 269 110 601.0 0 110 270 252 601.0 0 252 271 316 601.0 0 316 272 279 601.0 0 279 273 208 601.0 0 208 274 135 601.0 0 135 275 113 601.0 0 113 276 128 601.0 0 128 277 146 601.0 0 146 278 156 601.0 0 156 279 229 601.0 0 229 280 123 601.0 0 123 281 80 601.0 0 80 282 64 601.0 0 64 283 94 601.0 0 94 284 105 601.0 0 105 285 64 601.0 0 64 286 116 601.0 0 116 287 138 601.0 0 138 288 165 601.0 0 165 289 126 601.0 0 126 290 88 601.0 0 88 291 119 601.0 0 119 292 660 601.0 0 660 293 1385 601.0 0 1385 294 799 601.0 0 799 295 242 601.0 0 242 296 80 601.0 0 80 297 76 601.0 0 76 298 85 601.0 0 85 299 107 601.0 0 107 300 100 601.0 0 100 301 482 601.0 0 482 Finished in 1.87 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 35,609 Reads with adapters: 14,190 (39.8%) Reads that were too short: 22 (0.1%) Reads written (passing filters): 14,168 (39.8%) Total basepairs processed: 8,798,623 bp Total written (filtered): 3,858,515 bp (43.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14190 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 2 0 1 19 84 0.0 3 0 64 8 8 4 20 7470 0.0 4 6778 532 116 31 13 21 153 0.0 4 18 91 20 14 10 22 6230 0.0 4 5551 510 116 37 16 23 41 0.0 4 6 12 1 18 4 24 3 0.0 4 0 0 3 25 26 0.0 4 0 0 0 14 12 40 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 65 2 0.0 4 0 1 0 0 1 67 2 0.0 4 0 0 2 82 2 0.0 4 0 0 0 0 2 84 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 1 102 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 154 0.0 4 0 0 0 0 154 128 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.50 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 14,168 Reads with adapters: 2,050 (14.5%) Reads written (passing filters): 14,168 (100.0%) Total basepairs processed: 3,858,515 bp Total written (filtered): 3,833,002 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2050 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.4% C: 12.0% G: 81.4% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 63 221.4 0 63 4 14 55.3 0 14 7 1 0.9 0 1 9 18 0.1 0 4 14 10 409 0.0 1 32 377 11 1382 0.0 1 60 1322 12 135 0.0 1 3 132 13 6 0.0 1 0 6 14 1 0.0 1 0 1 151 4 0.0 1 1 3 152 2 0.0 1 1 1 153 1 0.0 1 1 209 8 0.0 1 5 3 210 3 0.0 1 3 212 1 0.0 1 1 260 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.