This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.67 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 135,410 (5.5%) Reads that were too short: 98,165 (4.0%) Reads written (passing filters): 37,245 (1.5%) Total basepairs processed: 740,966,583 bp Total written (filtered): 6,453,404 bp (0.9%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 135410 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 159 601.0 0 159 7 150 601.0 0 150 8 2075 601.0 0 2075 9 1814 601.0 0 1814 10 118 601.0 0 118 11 208 601.0 0 208 12 55 601.0 0 55 13 63 601.0 0 63 14 100 601.0 0 100 15 68 601.0 0 68 16 63 601.0 0 63 17 78 601.0 0 78 18 67 601.0 0 67 19 60 601.0 0 60 20 60 601.0 0 60 21 66 601.0 0 66 22 53 601.0 0 53 23 41 601.0 0 41 24 65 601.0 0 65 25 39 601.0 0 39 26 76 601.0 0 76 27 51 601.0 0 51 28 52 601.0 0 52 29 44 601.0 0 44 30 63 601.0 0 63 31 59 601.0 0 59 32 43 601.0 0 43 33 54 601.0 0 54 34 48 601.0 0 48 35 52 601.0 0 52 36 35 601.0 0 35 37 72 601.0 0 72 38 102 601.0 0 102 39 52 601.0 0 52 40 59 601.0 0 59 41 68 601.0 0 68 42 47 601.0 0 47 43 41 601.0 0 41 44 52 601.0 0 52 45 47 601.0 0 47 46 83 601.0 0 83 47 99 601.0 0 99 48 119 601.0 0 119 49 63 601.0 0 63 50 62 601.0 0 62 51 50 601.0 0 50 52 60 601.0 0 60 53 79 601.0 0 79 54 40 601.0 0 40 55 55 601.0 0 55 56 46 601.0 0 46 57 72 601.0 0 72 58 85 601.0 0 85 59 52 601.0 0 52 60 84 601.0 0 84 61 39 601.0 0 39 62 71 601.0 0 71 63 34 601.0 0 34 64 67 601.0 0 67 65 40 601.0 0 40 66 55 601.0 0 55 67 187 601.0 0 187 68 157 601.0 0 157 69 141 601.0 0 141 70 52 601.0 0 52 71 75 601.0 0 75 72 63 601.0 0 63 73 59 601.0 0 59 74 73 601.0 0 73 75 108 601.0 0 108 76 54 601.0 0 54 77 79 601.0 0 79 78 120 601.0 0 120 79 90 601.0 0 90 80 60 601.0 0 60 81 67 601.0 0 67 82 69 601.0 0 69 83 52 601.0 0 52 84 53 601.0 0 53 85 62 601.0 0 62 86 78 601.0 0 78 87 52 601.0 0 52 88 61 601.0 0 61 89 53 601.0 0 53 90 41 601.0 0 41 91 58 601.0 0 58 92 51 601.0 0 51 93 66 601.0 0 66 94 55 601.0 0 55 95 103 601.0 0 103 96 67 601.0 0 67 97 318 601.0 0 318 98 122 601.0 0 122 99 124 601.0 0 124 100 57 601.0 0 57 101 69 601.0 0 69 102 46 601.0 0 46 103 77 601.0 0 77 104 64 601.0 0 64 105 69 601.0 0 69 106 136 601.0 0 136 107 136 601.0 0 136 108 90 601.0 0 90 109 97 601.0 0 97 110 66 601.0 0 66 111 78 601.0 0 78 112 91 601.0 0 91 113 77 601.0 0 77 114 91 601.0 0 91 115 87 601.0 0 87 116 90 601.0 0 90 117 90 601.0 0 90 118 89 601.0 0 89 119 168 601.0 0 168 120 117 601.0 0 117 121 102 601.0 0 102 122 82 601.0 0 82 123 112 601.0 0 112 124 114 601.0 0 114 125 668 601.0 0 668 126 254 601.0 0 254 127 493 601.0 0 493 128 121 601.0 0 121 129 117 601.0 0 117 130 89 601.0 0 89 131 85 601.0 0 85 132 86 601.0 0 86 133 142 601.0 0 142 134 199 601.0 0 199 135 502 601.0 0 502 136 196 601.0 0 196 137 101 601.0 0 101 138 145 601.0 0 145 139 130 601.0 0 130 140 73 601.0 0 73 141 106 601.0 0 106 142 98 601.0 0 98 143 99 601.0 0 99 144 92 601.0 0 92 145 738 601.0 0 738 146 453 601.0 0 453 147 398 601.0 0 398 148 157 601.0 0 157 149 90 601.0 0 90 150 101 601.0 0 101 151 86 601.0 0 86 152 91 601.0 0 91 153 86 601.0 0 86 154 136 601.0 0 136 155 145 601.0 0 145 156 133 601.0 0 133 157 125 601.0 0 125 158 141 601.0 0 141 159 221 601.0 0 221 160 118 601.0 0 118 161 117 601.0 0 117 162 112 601.0 0 112 163 145 601.0 0 145 164 131 601.0 0 131 165 188 601.0 0 188 166 255 601.0 0 255 167 305 601.0 0 305 168 124 601.0 0 124 169 115 601.0 0 115 170 202 601.0 0 202 171 136 601.0 0 136 172 173 601.0 0 173 173 512 601.0 0 512 174 1183 601.0 0 1183 175 2124 601.0 0 2124 176 1689 601.0 0 1689 177 109 601.0 0 109 178 122 601.0 0 122 179 136 601.0 0 136 180 211 601.0 0 211 181 595 601.0 0 595 182 552 601.0 0 552 183 606 601.0 0 606 184 282 601.0 0 282 185 263 601.0 0 263 186 2021 601.0 0 2021 187 1361 601.0 0 1361 188 1461 601.0 0 1461 189 246 601.0 0 246 190 133 601.0 0 133 191 135 601.0 0 135 192 218 601.0 0 218 193 199 601.0 0 199 194 153 601.0 0 153 195 168 601.0 0 168 196 106 601.0 0 106 197 119 601.0 0 119 198 73 601.0 0 73 199 126 601.0 0 126 200 113 601.0 0 113 201 172 601.0 0 172 202 107 601.0 0 107 203 158 601.0 0 158 204 152 601.0 0 152 205 158 601.0 0 158 206 827 601.0 0 827 207 791 601.0 0 791 208 643 601.0 0 643 209 156 601.0 0 156 210 167 601.0 0 167 211 320 601.0 0 320 212 470 601.0 0 470 213 204 601.0 0 204 214 304 601.0 0 304 215 309 601.0 0 309 216 198 601.0 0 198 217 205 601.0 0 205 218 259 601.0 0 259 219 309 601.0 0 309 220 360 601.0 0 360 221 375 601.0 0 375 222 454 601.0 0 454 223 412 601.0 0 412 224 818 601.0 0 818 225 15585 601.0 0 15585 226 7378 601.0 0 7378 227 5762 601.0 0 5762 228 223 601.0 0 223 229 355 601.0 0 355 230 3292 601.0 0 3292 231 3339 601.0 0 3339 232 1436 601.0 0 1436 233 415 601.0 0 415 234 280 601.0 0 280 235 270 601.0 0 270 236 349 601.0 0 349 237 244 601.0 0 244 238 198 601.0 0 198 239 134 601.0 0 134 240 145 601.0 0 145 241 219 601.0 0 219 242 201 601.0 0 201 243 152 601.0 0 152 244 183 601.0 0 183 245 183 601.0 0 183 246 461 601.0 0 461 247 495 601.0 0 495 248 235 601.0 0 235 249 177 601.0 0 177 250 885 601.0 0 885 251 636 601.0 0 636 252 1038 601.0 0 1038 253 1163 601.0 0 1163 254 2277 601.0 0 2277 255 1413 601.0 0 1413 256 90 601.0 0 90 257 97 601.0 0 97 258 117 601.0 0 117 259 146 601.0 0 146 260 103 601.0 0 103 261 132 601.0 0 132 262 272 601.0 0 272 263 256 601.0 0 256 264 251 601.0 0 251 265 261 601.0 0 261 266 155 601.0 0 155 267 177 601.0 0 177 268 256 601.0 0 256 269 280 601.0 0 280 270 191 601.0 0 191 271 478 601.0 0 478 272 306 601.0 0 306 273 253 601.0 0 253 274 233 601.0 0 233 275 478 601.0 0 478 276 361 601.0 0 361 277 344 601.0 0 344 278 448 601.0 0 448 279 1042 601.0 0 1042 280 3858 601.0 0 3858 281 5398 601.0 0 5398 282 6026 601.0 0 6026 283 2282 601.0 0 2282 284 395 601.0 0 395 285 648 601.0 0 648 286 303 601.0 0 303 287 283 601.0 0 283 288 238 601.0 0 238 289 305 601.0 0 305 290 436 601.0 0 436 291 275 601.0 0 275 292 256 601.0 0 256 293 171 601.0 0 171 294 396 601.0 0 396 295 510 601.0 0 510 296 682 601.0 0 682 297 904 601.0 0 904 298 1348 601.0 0 1348 299 1455 601.0 0 1455 300 2273 601.0 0 2273 301 5717 601.0 0 5717 Finished in 1.47 s (39 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 37,245 Reads with adapters: 1,892 (5.1%) Reads that were too short: 22 (0.1%) Reads written (passing filters): 1,870 (5.0%) Total basepairs processed: 6,453,404 bp Total written (filtered): 506,006 bp (7.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1892 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 1 1 19 18 0.0 3 0 15 2 1 20 1787 0.0 4 1637 116 21 12 1 21 14 0.0 4 3 4 4 3 22 7 0.0 4 4 1 1 1 23 16 0.0 4 0 1 2 7 6 26 1 0.0 4 0 0 0 0 1 41 1 0.0 4 1 42 1 0.0 4 0 1 43 1 0.0 4 1 63 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 1 83 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 97 2 0.0 4 0 0 0 0 2 100 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 127 19 0.0 4 0 0 0 0 19 128 3 0.0 4 0 0 0 0 3 162 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 1 234 1 0.0 4 0 0 0 0 1 239 2 0.0 4 0 0 0 0 2 256 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (37 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 1,870 Reads with adapters: 444 (23.7%) Reads written (passing filters): 1,870 (100.0%) Total basepairs processed: 506,006 bp Total written (filtered): 501,192 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 444 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.8% C: 7.0% G: 82.7% T: 3.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 29.2 0 1 9 87 0.0 0 9 78 10 219 0.0 1 15 204 11 110 0.0 1 10 100 12 3 0.0 1 0 3 13 1 0.0 1 0 1 18 6 0.0 1 2 4 22 7 0.0 1 3 4 23 8 0.0 1 6 2 26 1 0.0 1 1 107 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.