This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.63 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 128,325 (5.2%) Reads that were too short: 99,840 (4.1%) Reads written (passing filters): 28,485 (1.2%) Total basepairs processed: 740,966,583 bp Total written (filtered): 5,434,159 bp (0.7%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 128325 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 165 601.0 0 165 7 122 601.0 0 122 8 2124 601.0 0 2124 9 2059 601.0 0 2059 10 120 601.0 0 120 11 91 601.0 0 91 12 59 601.0 0 59 13 113 601.0 0 113 14 134 601.0 0 134 15 71 601.0 0 71 16 75 601.0 0 75 17 94 601.0 0 94 18 139 601.0 0 139 19 73 601.0 0 73 20 87 601.0 0 87 21 81 601.0 0 81 22 74 601.0 0 74 23 53 601.0 0 53 24 85 601.0 0 85 25 66 601.0 0 66 26 66 601.0 0 66 27 55 601.0 0 55 28 54 601.0 0 54 29 44 601.0 0 44 30 68 601.0 0 68 31 104 601.0 0 104 32 75 601.0 0 75 33 74 601.0 0 74 34 68 601.0 0 68 35 167 601.0 0 167 36 44 601.0 0 44 37 62 601.0 0 62 38 119 601.0 0 119 39 65 601.0 0 65 40 56 601.0 0 56 41 66 601.0 0 66 42 47 601.0 0 47 43 62 601.0 0 62 44 78 601.0 0 78 45 78 601.0 0 78 46 73 601.0 0 73 47 89 601.0 0 89 48 218 601.0 0 218 49 78 601.0 0 78 50 96 601.0 0 96 51 71 601.0 0 71 52 81 601.0 0 81 53 77 601.0 0 77 54 39 601.0 0 39 55 53 601.0 0 53 56 42 601.0 0 42 57 70 601.0 0 70 58 85 601.0 0 85 59 50 601.0 0 50 60 75 601.0 0 75 61 67 601.0 0 67 62 64 601.0 0 64 63 42 601.0 0 42 64 55 601.0 0 55 65 51 601.0 0 51 66 70 601.0 0 70 67 197 601.0 0 197 68 70 601.0 0 70 69 61 601.0 0 61 70 50 601.0 0 50 71 92 601.0 0 92 72 78 601.0 0 78 73 50 601.0 0 50 74 57 601.0 0 57 75 110 601.0 0 110 76 57 601.0 0 57 77 82 601.0 0 82 78 109 601.0 0 109 79 67 601.0 0 67 80 53 601.0 0 53 81 66 601.0 0 66 82 75 601.0 0 75 83 61 601.0 0 61 84 61 601.0 0 61 85 62 601.0 0 62 86 59 601.0 0 59 87 57 601.0 0 57 88 73 601.0 0 73 89 66 601.0 0 66 90 61 601.0 0 61 91 60 601.0 0 60 92 67 601.0 0 67 93 63 601.0 0 63 94 63 601.0 0 63 95 81 601.0 0 81 96 67 601.0 0 67 97 166 601.0 0 166 98 104 601.0 0 104 99 91 601.0 0 91 100 52 601.0 0 52 101 59 601.0 0 59 102 54 601.0 0 54 103 59 601.0 0 59 104 64 601.0 0 64 105 73 601.0 0 73 106 124 601.0 0 124 107 87 601.0 0 87 108 63 601.0 0 63 109 121 601.0 0 121 110 66 601.0 0 66 111 76 601.0 0 76 112 90 601.0 0 90 113 79 601.0 0 79 114 69 601.0 0 69 115 64 601.0 0 64 116 104 601.0 0 104 117 99 601.0 0 99 118 85 601.0 0 85 119 120 601.0 0 120 120 146 601.0 0 146 121 90 601.0 0 90 122 80 601.0 0 80 123 100 601.0 0 100 124 87 601.0 0 87 125 623 601.0 0 623 126 187 601.0 0 187 127 446 601.0 0 446 128 65 601.0 0 65 129 75 601.0 0 75 130 77 601.0 0 77 131 89 601.0 0 89 132 67 601.0 0 67 133 79 601.0 0 79 134 116 601.0 0 116 135 381 601.0 0 381 136 129 601.0 0 129 137 72 601.0 0 72 138 99 601.0 0 99 139 80 601.0 0 80 140 77 601.0 0 77 141 85 601.0 0 85 142 91 601.0 0 91 143 82 601.0 0 82 144 73 601.0 0 73 145 397 601.0 0 397 146 207 601.0 0 207 147 249 601.0 0 249 148 125 601.0 0 125 149 91 601.0 0 91 150 63 601.0 0 63 151 84 601.0 0 84 152 61 601.0 0 61 153 77 601.0 0 77 154 67 601.0 0 67 155 82 601.0 0 82 156 87 601.0 0 87 157 102 601.0 0 102 158 113 601.0 0 113 159 109 601.0 0 109 160 80 601.0 0 80 161 79 601.0 0 79 162 98 601.0 0 98 163 104 601.0 0 104 164 110 601.0 0 110 165 110 601.0 0 110 166 121 601.0 0 121 167 127 601.0 0 127 168 103 601.0 0 103 169 103 601.0 0 103 170 208 601.0 0 208 171 120 601.0 0 120 172 180 601.0 0 180 173 364 601.0 0 364 174 675 601.0 0 675 175 1128 601.0 0 1128 176 738 601.0 0 738 177 95 601.0 0 95 178 86 601.0 0 86 179 123 601.0 0 123 180 136 601.0 0 136 181 428 601.0 0 428 182 384 601.0 0 384 183 429 601.0 0 429 184 205 601.0 0 205 185 113 601.0 0 113 186 684 601.0 0 684 187 703 601.0 0 703 188 872 601.0 0 872 189 168 601.0 0 168 190 84 601.0 0 84 191 106 601.0 0 106 192 135 601.0 0 135 193 123 601.0 0 123 194 107 601.0 0 107 195 99 601.0 0 99 196 93 601.0 0 93 197 71 601.0 0 71 198 69 601.0 0 69 199 83 601.0 0 83 200 82 601.0 0 82 201 122 601.0 0 122 202 106 601.0 0 106 203 140 601.0 0 140 204 84 601.0 0 84 205 91 601.0 0 91 206 231 601.0 0 231 207 367 601.0 0 367 208 195 601.0 0 195 209 133 601.0 0 133 210 139 601.0 0 139 211 324 601.0 0 324 212 517 601.0 0 517 213 157 601.0 0 157 214 287 601.0 0 287 215 334 601.0 0 334 216 188 601.0 0 188 217 162 601.0 0 162 218 262 601.0 0 262 219 231 601.0 0 231 220 242 601.0 0 242 221 243 601.0 0 243 222 318 601.0 0 318 223 305 601.0 0 305 224 679 601.0 0 679 225 18419 601.0 0 18419 226 8260 601.0 0 8260 227 6394 601.0 0 6394 228 115 601.0 0 115 229 316 601.0 0 316 230 1366 601.0 0 1366 231 1757 601.0 0 1757 232 515 601.0 0 515 233 174 601.0 0 174 234 152 601.0 0 152 235 130 601.0 0 130 236 136 601.0 0 136 237 139 601.0 0 139 238 118 601.0 0 118 239 82 601.0 0 82 240 92 601.0 0 92 241 146 601.0 0 146 242 117 601.0 0 117 243 124 601.0 0 124 244 175 601.0 0 175 245 154 601.0 0 154 246 286 601.0 0 286 247 346 601.0 0 346 248 165 601.0 0 165 249 158 601.0 0 158 250 709 601.0 0 709 251 420 601.0 0 420 252 653 601.0 0 653 253 505 601.0 0 505 254 1045 601.0 0 1045 255 530 601.0 0 530 256 79 601.0 0 79 257 99 601.0 0 99 258 131 601.0 0 131 259 128 601.0 0 128 260 75 601.0 0 75 261 88 601.0 0 88 262 118 601.0 0 118 263 136 601.0 0 136 264 177 601.0 0 177 265 477 601.0 0 477 266 201 601.0 0 201 267 193 601.0 0 193 268 207 601.0 0 207 269 196 601.0 0 196 270 163 601.0 0 163 271 342 601.0 0 342 272 330 601.0 0 330 273 224 601.0 0 224 274 335 601.0 0 335 275 944 601.0 0 944 276 459 601.0 0 459 277 158 601.0 0 158 278 207 601.0 0 207 279 355 601.0 0 355 280 1349 601.0 0 1349 281 1515 601.0 0 1515 282 1601 601.0 0 1601 283 906 601.0 0 906 284 684 601.0 0 684 285 1391 601.0 0 1391 286 681 601.0 0 681 287 512 601.0 0 512 288 390 601.0 0 390 289 441 601.0 0 441 290 735 601.0 0 735 291 531 601.0 0 531 292 449 601.0 0 449 293 370 601.0 0 370 294 506 601.0 0 506 295 731 601.0 0 731 296 1201 601.0 0 1201 297 1833 601.0 0 1833 298 2927 601.0 0 2927 299 3682 601.0 0 3682 300 5780 601.0 0 5780 301 16370 601.0 0 16370 Finished in 1.09 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 28,485 Reads with adapters: 2,173 (7.6%) Reads that were too short: 47 (0.2%) Reads written (passing filters): 2,126 (7.5%) Total basepairs processed: 5,434,159 bp Total written (filtered): 580,726 bp (10.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2173 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 17 0.0 3 0 17 17 2054 0.0 3 2013 37 4 18 6 0.0 3 1 5 19 3 0.0 3 2 0 0 1 20 8 0.0 3 0 0 0 8 29 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 46 11 0.0 3 0 0 0 11 49 3 0.0 3 0 0 0 3 51 1 0.0 3 0 0 0 1 58 3 0.0 3 0 0 0 3 66 10 0.0 3 0 0 0 10 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 94 4 0.0 3 0 0 0 4 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 100 3 0.0 3 0 0 0 3 113 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 185 1 0.0 3 1 186 1 0.0 3 1 187 16 0.0 3 0 0 0 16 195 2 0.0 3 0 0 0 2 204 2 0.0 3 2 256 1 0.0 3 1 258 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 2,126 Reads with adapters: 5 (0.2%) Reads written (passing filters): 2,126 (100.0%) Total basepairs processed: 580,726 bp Total written (filtered): 580,708 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 40.0% C: 20.0% G: 0.0% T: 40.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 33.2 0 3 4 1 8.3 0 1 5 1 2.1 0 1