This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.15 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 45,362 (1.8%) Reads that were too short: 24,185 (1.0%) Reads written (passing filters): 21,177 (0.9%) Total basepairs processed: 740,966,583 bp Total written (filtered): 4,085,579 bp (0.6%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 45362 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 58 601.0 0 58 7 44 601.0 0 44 8 64 601.0 0 64 9 55 601.0 0 55 10 75 601.0 0 75 11 55 601.0 0 55 12 62 601.0 0 62 13 67 601.0 0 67 14 70 601.0 0 70 15 44 601.0 0 44 16 46 601.0 0 46 17 61 601.0 0 61 18 49 601.0 0 49 19 69 601.0 0 69 20 51 601.0 0 51 21 56 601.0 0 56 22 67 601.0 0 67 23 52 601.0 0 52 24 41 601.0 0 41 25 52 601.0 0 52 26 88 601.0 0 88 27 61 601.0 0 61 28 49 601.0 0 49 29 69 601.0 0 69 30 61 601.0 0 61 31 65 601.0 0 65 32 67 601.0 0 67 33 54 601.0 0 54 34 46 601.0 0 46 35 64 601.0 0 64 36 38 601.0 0 38 37 52 601.0 0 52 38 89 601.0 0 89 39 1193 601.0 0 1193 40 972 601.0 0 972 41 560 601.0 0 560 42 107 601.0 0 107 43 108 601.0 0 108 44 55 601.0 0 55 45 49 601.0 0 49 46 68 601.0 0 68 47 44 601.0 0 44 48 30 601.0 0 30 49 47 601.0 0 47 50 79 601.0 0 79 51 52 601.0 0 52 52 94 601.0 0 94 53 70 601.0 0 70 54 198 601.0 0 198 55 62 601.0 0 62 56 55 601.0 0 55 57 71 601.0 0 71 58 57 601.0 0 57 59 47 601.0 0 47 60 55 601.0 0 55 61 63 601.0 0 63 62 46 601.0 0 46 63 52 601.0 0 52 64 63 601.0 0 63 65 68 601.0 0 68 66 61 601.0 0 61 67 138 601.0 0 138 68 57 601.0 0 57 69 39 601.0 0 39 70 61 601.0 0 61 71 110 601.0 0 110 72 67 601.0 0 67 73 60 601.0 0 60 74 46 601.0 0 46 75 53 601.0 0 53 76 77 601.0 0 77 77 81 601.0 0 81 78 57 601.0 0 57 79 66 601.0 0 66 80 65 601.0 0 65 81 59 601.0 0 59 82 59 601.0 0 59 83 75 601.0 0 75 84 83 601.0 0 83 85 94 601.0 0 94 86 86 601.0 0 86 87 60 601.0 0 60 88 62 601.0 0 62 89 62 601.0 0 62 90 62 601.0 0 62 91 90 601.0 0 90 92 103 601.0 0 103 93 104 601.0 0 104 94 81 601.0 0 81 95 88 601.0 0 88 96 86 601.0 0 86 97 101 601.0 0 101 98 597 601.0 0 597 99 477 601.0 0 477 100 263 601.0 0 263 101 99 601.0 0 99 102 81 601.0 0 81 103 88 601.0 0 88 104 64 601.0 0 64 105 113 601.0 0 113 106 95 601.0 0 95 107 94 601.0 0 94 108 65 601.0 0 65 109 73 601.0 0 73 110 97 601.0 0 97 111 256 601.0 0 256 112 234 601.0 0 234 113 130 601.0 0 130 114 66 601.0 0 66 115 71 601.0 0 71 116 63 601.0 0 63 117 67 601.0 0 67 118 56 601.0 0 56 119 66 601.0 0 66 120 74 601.0 0 74 121 74 601.0 0 74 122 55 601.0 0 55 123 62 601.0 0 62 124 110 601.0 0 110 125 134 601.0 0 134 126 69 601.0 0 69 127 64 601.0 0 64 128 47 601.0 0 47 129 71 601.0 0 71 130 85 601.0 0 85 131 106 601.0 0 106 132 101 601.0 0 101 133 252 601.0 0 252 134 79 601.0 0 79 135 83 601.0 0 83 136 62 601.0 0 62 137 113 601.0 0 113 138 112 601.0 0 112 139 141 601.0 0 141 140 84 601.0 0 84 141 88 601.0 0 88 142 99 601.0 0 99 143 130 601.0 0 130 144 123 601.0 0 123 145 225 601.0 0 225 146 91 601.0 0 91 147 81 601.0 0 81 148 97 601.0 0 97 149 68 601.0 0 68 150 75 601.0 0 75 151 69 601.0 0 69 152 79 601.0 0 79 153 79 601.0 0 79 154 73 601.0 0 73 155 58 601.0 0 58 156 91 601.0 0 91 157 75 601.0 0 75 158 123 601.0 0 123 159 205 601.0 0 205 160 197 601.0 0 197 161 76 601.0 0 76 162 107 601.0 0 107 163 80 601.0 0 80 164 101 601.0 0 101 165 74 601.0 0 74 166 70 601.0 0 70 167 91 601.0 0 91 168 107 601.0 0 107 169 83 601.0 0 83 170 143 601.0 0 143 171 142 601.0 0 142 172 177 601.0 0 177 173 109 601.0 0 109 174 70 601.0 0 70 175 49 601.0 0 49 176 89 601.0 0 89 177 156 601.0 0 156 178 231 601.0 0 231 179 254 601.0 0 254 180 163 601.0 0 163 181 111 601.0 0 111 182 72 601.0 0 72 183 178 601.0 0 178 184 221 601.0 0 221 185 376 601.0 0 376 186 134 601.0 0 134 187 149 601.0 0 149 188 158 601.0 0 158 189 177 601.0 0 177 190 117 601.0 0 117 191 133 601.0 0 133 192 159 601.0 0 159 193 88 601.0 0 88 194 144 601.0 0 144 195 87 601.0 0 87 196 85 601.0 0 85 197 60 601.0 0 60 198 84 601.0 0 84 199 94 601.0 0 94 200 63 601.0 0 63 201 97 601.0 0 97 202 95 601.0 0 95 203 98 601.0 0 98 204 111 601.0 0 111 205 93 601.0 0 93 206 92 601.0 0 92 207 85 601.0 0 85 208 90 601.0 0 90 209 85 601.0 0 85 210 152 601.0 0 152 211 242 601.0 0 242 212 209 601.0 0 209 213 219 601.0 0 219 214 852 601.0 0 852 215 778 601.0 0 778 216 587 601.0 0 587 217 86 601.0 0 86 218 70 601.0 0 70 219 80 601.0 0 80 220 93 601.0 0 93 221 98 601.0 0 98 222 162 601.0 0 162 223 591 601.0 0 591 224 473 601.0 0 473 225 841 601.0 0 841 226 98 601.0 0 98 227 92 601.0 0 92 228 101 601.0 0 101 229 110 601.0 0 110 230 122 601.0 0 122 231 71 601.0 0 71 232 84 601.0 0 84 233 84 601.0 0 84 234 92 601.0 0 92 235 109 601.0 0 109 236 118 601.0 0 118 237 131 601.0 0 131 238 260 601.0 0 260 239 282 601.0 0 282 240 297 601.0 0 297 241 141 601.0 0 141 242 195 601.0 0 195 243 141 601.0 0 141 244 75 601.0 0 75 245 92 601.0 0 92 246 88 601.0 0 88 247 86 601.0 0 86 248 71 601.0 0 71 249 88 601.0 0 88 250 139 601.0 0 139 251 128 601.0 0 128 252 114 601.0 0 114 253 113 601.0 0 113 254 125 601.0 0 125 255 111 601.0 0 111 256 227 601.0 0 227 257 231 601.0 0 231 258 134 601.0 0 134 259 149 601.0 0 149 260 222 601.0 0 222 261 110 601.0 0 110 262 132 601.0 0 132 263 301 601.0 0 301 264 341 601.0 0 341 265 257 601.0 0 257 266 92 601.0 0 92 267 90 601.0 0 90 268 86 601.0 0 86 269 103 601.0 0 103 270 116 601.0 0 116 271 345 601.0 0 345 272 1664 601.0 0 1664 273 1635 601.0 0 1635 274 1785 601.0 0 1785 275 134 601.0 0 134 276 342 601.0 0 342 277 339 601.0 0 339 278 312 601.0 0 312 279 157 601.0 0 157 280 151 601.0 0 151 281 94 601.0 0 94 282 215 601.0 0 215 283 605 601.0 0 605 284 834 601.0 0 834 285 523 601.0 0 523 286 344 601.0 0 344 287 123 601.0 0 123 288 119 601.0 0 119 289 124 601.0 0 124 290 80 601.0 0 80 291 93 601.0 0 93 292 82 601.0 0 82 293 93 601.0 0 93 294 78 601.0 0 78 295 80 601.0 0 80 296 81 601.0 0 81 297 141 601.0 0 141 298 257 601.0 0 257 299 314 601.0 0 314 300 254 601.0 0 254 301 156 601.0 0 156 Finished in 0.89 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 21,177 Reads with adapters: 62 (0.3%) Reads that were too short: 17 (0.1%) Reads written (passing filters): 45 (0.2%) Total basepairs processed: 4,085,579 bp Total written (filtered): 9,951 bp (0.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 62 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 20 6 0.0 4 3 3 21 3 0.0 4 0 0 1 2 22 5 0.0 4 1 1 1 1 1 23 8 0.0 4 3 3 2 25 2 0.0 4 0 0 0 0 2 36 15 0.0 4 0 0 0 0 15 59 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 82 3 0.0 4 0 0 0 0 3 85 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 1 157 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 230 2 0.0 4 0 0 0 0 2 231 2 0.0 4 0 0 0 1 1 233 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (222 us/read; 0.27 M reads/minute). === Summary === Total reads processed: 45 Reads with adapters: 2 (4.4%) Reads written (passing filters): 45 (100.0%) Total basepairs processed: 9,951 bp Total written (filtered): 9,944 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 50.0% C: 0.0% G: 50.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 0.7 0 1 4 1 0.2 0 1