This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.33 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 40,701 (1.7%) Reads that were too short: 20,492 (0.8%) Reads written (passing filters): 20,209 (0.8%) Total basepairs processed: 740,966,583 bp Total written (filtered): 3,938,196 bp (0.5%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 40701 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 85 601.0 0 85 7 77 601.0 0 77 8 89 601.0 0 89 9 67 601.0 0 67 10 78 601.0 0 78 11 77 601.0 0 77 12 89 601.0 0 89 13 77 601.0 0 77 14 93 601.0 0 93 15 80 601.0 0 80 16 59 601.0 0 59 17 71 601.0 0 71 18 95 601.0 0 95 19 82 601.0 0 82 20 86 601.0 0 86 21 97 601.0 0 97 22 92 601.0 0 92 23 78 601.0 0 78 24 60 601.0 0 60 25 68 601.0 0 68 26 62 601.0 0 62 27 62 601.0 0 62 28 69 601.0 0 69 29 87 601.0 0 87 30 98 601.0 0 98 31 90 601.0 0 90 32 97 601.0 0 97 33 71 601.0 0 71 34 86 601.0 0 86 35 83 601.0 0 83 36 76 601.0 0 76 37 58 601.0 0 58 38 87 601.0 0 87 39 450 601.0 0 450 40 453 601.0 0 453 41 295 601.0 0 295 42 129 601.0 0 129 43 123 601.0 0 123 44 68 601.0 0 68 45 65 601.0 0 65 46 105 601.0 0 105 47 75 601.0 0 75 48 51 601.0 0 51 49 78 601.0 0 78 50 68 601.0 0 68 51 79 601.0 0 79 52 127 601.0 0 127 53 94 601.0 0 94 54 266 601.0 0 266 55 66 601.0 0 66 56 88 601.0 0 88 57 103 601.0 0 103 58 93 601.0 0 93 59 73 601.0 0 73 60 95 601.0 0 95 61 62 601.0 0 62 62 67 601.0 0 67 63 59 601.0 0 59 64 65 601.0 0 65 65 104 601.0 0 104 66 96 601.0 0 96 67 318 601.0 0 318 68 102 601.0 0 102 69 67 601.0 0 67 70 87 601.0 0 87 71 123 601.0 0 123 72 94 601.0 0 94 73 92 601.0 0 92 74 74 601.0 0 74 75 94 601.0 0 94 76 91 601.0 0 91 77 76 601.0 0 76 78 61 601.0 0 61 79 93 601.0 0 93 80 85 601.0 0 85 81 67 601.0 0 67 82 71 601.0 0 71 83 82 601.0 0 82 84 90 601.0 0 90 85 83 601.0 0 83 86 103 601.0 0 103 87 72 601.0 0 72 88 74 601.0 0 74 89 55 601.0 0 55 90 90 601.0 0 90 91 85 601.0 0 85 92 101 601.0 0 101 93 101 601.0 0 101 94 71 601.0 0 71 95 79 601.0 0 79 96 102 601.0 0 102 97 85 601.0 0 85 98 288 601.0 0 288 99 255 601.0 0 255 100 172 601.0 0 172 101 82 601.0 0 82 102 75 601.0 0 75 103 88 601.0 0 88 104 85 601.0 0 85 105 114 601.0 0 114 106 117 601.0 0 117 107 109 601.0 0 109 108 78 601.0 0 78 109 76 601.0 0 76 110 87 601.0 0 87 111 163 601.0 0 163 112 172 601.0 0 172 113 95 601.0 0 95 114 70 601.0 0 70 115 65 601.0 0 65 116 71 601.0 0 71 117 84 601.0 0 84 118 63 601.0 0 63 119 56 601.0 0 56 120 76 601.0 0 76 121 102 601.0 0 102 122 58 601.0 0 58 123 56 601.0 0 56 124 109 601.0 0 109 125 98 601.0 0 98 126 92 601.0 0 92 127 82 601.0 0 82 128 60 601.0 0 60 129 84 601.0 0 84 130 101 601.0 0 101 131 68 601.0 0 68 132 105 601.0 0 105 133 268 601.0 0 268 134 69 601.0 0 69 135 105 601.0 0 105 136 68 601.0 0 68 137 97 601.0 0 97 138 88 601.0 0 88 139 83 601.0 0 83 140 70 601.0 0 70 141 80 601.0 0 80 142 79 601.0 0 79 143 117 601.0 0 117 144 102 601.0 0 102 145 207 601.0 0 207 146 88 601.0 0 88 147 89 601.0 0 89 148 125 601.0 0 125 149 94 601.0 0 94 150 104 601.0 0 104 151 94 601.0 0 94 152 67 601.0 0 67 153 94 601.0 0 94 154 96 601.0 0 96 155 77 601.0 0 77 156 84 601.0 0 84 157 100 601.0 0 100 158 111 601.0 0 111 159 195 601.0 0 195 160 205 601.0 0 205 161 79 601.0 0 79 162 83 601.0 0 83 163 78 601.0 0 78 164 72 601.0 0 72 165 68 601.0 0 68 166 90 601.0 0 90 167 86 601.0 0 86 168 82 601.0 0 82 169 83 601.0 0 83 170 144 601.0 0 144 171 123 601.0 0 123 172 115 601.0 0 115 173 106 601.0 0 106 174 106 601.0 0 106 175 59 601.0 0 59 176 106 601.0 0 106 177 144 601.0 0 144 178 163 601.0 0 163 179 160 601.0 0 160 180 134 601.0 0 134 181 118 601.0 0 118 182 97 601.0 0 97 183 193 601.0 0 193 184 145 601.0 0 145 185 356 601.0 0 356 186 120 601.0 0 120 187 113 601.0 0 113 188 136 601.0 0 136 189 138 601.0 0 138 190 102 601.0 0 102 191 104 601.0 0 104 192 111 601.0 0 111 193 77 601.0 0 77 194 113 601.0 0 113 195 100 601.0 0 100 196 90 601.0 0 90 197 78 601.0 0 78 198 62 601.0 0 62 199 90 601.0 0 90 200 88 601.0 0 88 201 94 601.0 0 94 202 72 601.0 0 72 203 87 601.0 0 87 204 117 601.0 0 117 205 87 601.0 0 87 206 83 601.0 0 83 207 89 601.0 0 89 208 71 601.0 0 71 209 56 601.0 0 56 210 103 601.0 0 103 211 149 601.0 0 149 212 93 601.0 0 93 213 124 601.0 0 124 214 313 601.0 0 313 215 297 601.0 0 297 216 288 601.0 0 288 217 78 601.0 0 78 218 61 601.0 0 61 219 86 601.0 0 86 220 87 601.0 0 87 221 89 601.0 0 89 222 148 601.0 0 148 223 609 601.0 0 609 224 549 601.0 0 549 225 919 601.0 0 919 226 101 601.0 0 101 227 78 601.0 0 78 228 72 601.0 0 72 229 116 601.0 0 116 230 148 601.0 0 148 231 64 601.0 0 64 232 83 601.0 0 83 233 71 601.0 0 71 234 79 601.0 0 79 235 86 601.0 0 86 236 78 601.0 0 78 237 77 601.0 0 77 238 211 601.0 0 211 239 257 601.0 0 257 240 364 601.0 0 364 241 92 601.0 0 92 242 144 601.0 0 144 243 78 601.0 0 78 244 85 601.0 0 85 245 97 601.0 0 97 246 69 601.0 0 69 247 80 601.0 0 80 248 87 601.0 0 87 249 84 601.0 0 84 250 139 601.0 0 139 251 73 601.0 0 73 252 91 601.0 0 91 253 91 601.0 0 91 254 113 601.0 0 113 255 124 601.0 0 124 256 110 601.0 0 110 257 115 601.0 0 115 258 96 601.0 0 96 259 105 601.0 0 105 260 190 601.0 0 190 261 103 601.0 0 103 262 86 601.0 0 86 263 272 601.0 0 272 264 387 601.0 0 387 265 255 601.0 0 255 266 107 601.0 0 107 267 79 601.0 0 79 268 84 601.0 0 84 269 110 601.0 0 110 270 146 601.0 0 146 271 282 601.0 0 282 272 1796 601.0 0 1796 273 1866 601.0 0 1866 274 2217 601.0 0 2217 275 157 601.0 0 157 276 135 601.0 0 135 277 177 601.0 0 177 278 140 601.0 0 140 279 113 601.0 0 113 280 106 601.0 0 106 281 92 601.0 0 92 282 125 601.0 0 125 283 317 601.0 0 317 284 440 601.0 0 440 285 333 601.0 0 333 286 241 601.0 0 241 287 103 601.0 0 103 288 119 601.0 0 119 289 95 601.0 0 95 290 100 601.0 0 100 291 66 601.0 0 66 292 62 601.0 0 62 293 66 601.0 0 66 294 87 601.0 0 87 295 77 601.0 0 77 296 81 601.0 0 81 297 107 601.0 0 107 298 188 601.0 0 188 299 244 601.0 0 244 300 201 601.0 0 201 301 127 601.0 0 127 Finished in 0.74 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 20,209 Reads with adapters: 78 (0.4%) Reads that were too short: 25 (0.1%) Reads written (passing filters): 53 (0.3%) Total basepairs processed: 3,938,196 bp Total written (filtered): 7,895 bp (0.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 78 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 8 0.0 3 7 0 1 24 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 34 2 0.0 3 0 0 0 2 39 8 0.0 3 0 0 0 8 45 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 73 4 0.0 3 0 0 0 4 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 89 4 0.0 3 0 0 0 4 92 3 0.0 3 0 0 0 3 97 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 120 7 0.0 3 0 0 0 7 126 1 0.0 3 0 0 0 1 130 1 0.0 3 1 132 4 0.0 3 0 0 0 4 133 1 0.0 3 0 0 0 1 135 6 0.0 3 0 0 0 6 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 245 3 0.0 3 0 0 0 3 250 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (189 us/read; 0.32 M reads/minute). === Summary === Total reads processed: 53 Reads with adapters: 1 (1.9%) Reads written (passing filters): 53 (100.0%) Total basepairs processed: 7,895 bp Total written (filtered): 7,892 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 0.8 0 1