This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 63.97 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 163,758 (6.7%) Reads that were too short: 25,021 (1.0%) Reads written (passing filters): 138,737 (5.6%) Total basepairs processed: 740,966,583 bp Total written (filtered): 24,359,059 bp (3.3%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 163758 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 278 601.0 0 278 7 312 601.0 0 312 8 198 601.0 0 198 9 253 601.0 0 253 10 377 601.0 0 377 11 189 601.0 0 189 12 153 601.0 0 153 13 102 601.0 0 102 14 119 601.0 0 119 15 154 601.0 0 154 16 128 601.0 0 128 17 218 601.0 0 218 18 166 601.0 0 166 19 129 601.0 0 129 20 142 601.0 0 142 21 880 601.0 0 880 22 501 601.0 0 501 23 381 601.0 0 381 24 2968 601.0 0 2968 25 1641 601.0 0 1641 26 847 601.0 0 847 27 169 601.0 0 169 28 162 601.0 0 162 29 165 601.0 0 165 30 114 601.0 0 114 31 123 601.0 0 123 32 143 601.0 0 143 33 149 601.0 0 149 34 97 601.0 0 97 35 120 601.0 0 120 36 128 601.0 0 128 37 124 601.0 0 124 38 121 601.0 0 121 39 132 601.0 0 132 40 152 601.0 0 152 41 155 601.0 0 155 42 143 601.0 0 143 43 124 601.0 0 124 44 130 601.0 0 130 45 143 601.0 0 143 46 118 601.0 0 118 47 148 601.0 0 148 48 133 601.0 0 133 49 134 601.0 0 134 50 128 601.0 0 128 51 159 601.0 0 159 52 177 601.0 0 177 53 163 601.0 0 163 54 182 601.0 0 182 55 154 601.0 0 154 56 167 601.0 0 167 57 154 601.0 0 154 58 170 601.0 0 170 59 152 601.0 0 152 60 151 601.0 0 151 61 905 601.0 0 905 62 782 601.0 0 782 63 323 601.0 0 323 64 137 601.0 0 137 65 149 601.0 0 149 66 153 601.0 0 153 67 162 601.0 0 162 68 167 601.0 0 167 69 420 601.0 0 420 70 478 601.0 0 478 71 243 601.0 0 243 72 195 601.0 0 195 73 204 601.0 0 204 74 197 601.0 0 197 75 201 601.0 0 201 76 137 601.0 0 137 77 140 601.0 0 140 78 207 601.0 0 207 79 163 601.0 0 163 80 246 601.0 0 246 81 242 601.0 0 242 82 353 601.0 0 353 83 3768 601.0 0 3768 84 4365 601.0 0 4365 85 1990 601.0 0 1990 86 156 601.0 0 156 87 141 601.0 0 141 88 180 601.0 0 180 89 137 601.0 0 137 90 124 601.0 0 124 91 149 601.0 0 149 92 141 601.0 0 141 93 139 601.0 0 139 94 147 601.0 0 147 95 121 601.0 0 121 96 163 601.0 0 163 97 163 601.0 0 163 98 208 601.0 0 208 99 322 601.0 0 322 100 156 601.0 0 156 101 186 601.0 0 186 102 243 601.0 0 243 103 201 601.0 0 201 104 186 601.0 0 186 105 259 601.0 0 259 106 195 601.0 0 195 107 272 601.0 0 272 108 242 601.0 0 242 109 280 601.0 0 280 110 334 601.0 0 334 111 592 601.0 0 592 112 8147 601.0 0 8147 113 7912 601.0 0 7912 114 4087 601.0 0 4087 115 211 601.0 0 211 116 164 601.0 0 164 117 171 601.0 0 171 118 249 601.0 0 249 119 308 601.0 0 308 120 3487 601.0 0 3487 121 1535 601.0 0 1535 122 1012 601.0 0 1012 123 367 601.0 0 367 124 432 601.0 0 432 125 4351 601.0 0 4351 126 6286 601.0 0 6286 127 1779 601.0 0 1779 128 183 601.0 0 183 129 221 601.0 0 221 130 183 601.0 0 183 131 191 601.0 0 191 132 147 601.0 0 147 133 194 601.0 0 194 134 218 601.0 0 218 135 162 601.0 0 162 136 489 601.0 0 489 137 296 601.0 0 296 138 247 601.0 0 247 139 222 601.0 0 222 140 212 601.0 0 212 141 270 601.0 0 270 142 921 601.0 0 921 143 698 601.0 0 698 144 614 601.0 0 614 145 354 601.0 0 354 146 418 601.0 0 418 147 4442 601.0 0 4442 148 4288 601.0 0 4288 149 2257 601.0 0 2257 150 980 601.0 0 980 151 504 601.0 0 504 152 421 601.0 0 421 153 666 601.0 0 666 154 366 601.0 0 366 155 198 601.0 0 198 156 264 601.0 0 264 157 242 601.0 0 242 158 175 601.0 0 175 159 224 601.0 0 224 160 183 601.0 0 183 161 216 601.0 0 216 162 243 601.0 0 243 163 639 601.0 0 639 164 466 601.0 0 466 165 794 601.0 0 794 166 1702 601.0 0 1702 167 1426 601.0 0 1426 168 1342 601.0 0 1342 169 789 601.0 0 789 170 744 601.0 0 744 171 3441 601.0 0 3441 172 2650 601.0 0 2650 173 1618 601.0 0 1618 174 446 601.0 0 446 175 340 601.0 0 340 176 246 601.0 0 246 177 353 601.0 0 353 178 269 601.0 0 269 179 217 601.0 0 217 180 222 601.0 0 222 181 285 601.0 0 285 182 443 601.0 0 443 183 2055 601.0 0 2055 184 1826 601.0 0 1826 185 3399 601.0 0 3399 186 487 601.0 0 487 187 580 601.0 0 580 188 569 601.0 0 569 189 305 601.0 0 305 190 301 601.0 0 301 191 2504 601.0 0 2504 192 3045 601.0 0 3045 193 3069 601.0 0 3069 194 851 601.0 0 851 195 362 601.0 0 362 196 359 601.0 0 359 197 223 601.0 0 223 198 173 601.0 0 173 199 306 601.0 0 306 200 339 601.0 0 339 201 361 601.0 0 361 202 483 601.0 0 483 203 159 601.0 0 159 204 140 601.0 0 140 205 210 601.0 0 210 206 185 601.0 0 185 207 298 601.0 0 298 208 262 601.0 0 262 209 184 601.0 0 184 210 156 601.0 0 156 211 159 601.0 0 159 212 133 601.0 0 133 213 144 601.0 0 144 214 137 601.0 0 137 215 188 601.0 0 188 216 249 601.0 0 249 217 317 601.0 0 317 218 284 601.0 0 284 219 256 601.0 0 256 220 235 601.0 0 235 221 150 601.0 0 150 222 169 601.0 0 169 223 141 601.0 0 141 224 172 601.0 0 172 225 291 601.0 0 291 226 653 601.0 0 653 227 457 601.0 0 457 228 490 601.0 0 490 229 421 601.0 0 421 230 771 601.0 0 771 231 176 601.0 0 176 232 161 601.0 0 161 233 148 601.0 0 148 234 156 601.0 0 156 235 153 601.0 0 153 236 144 601.0 0 144 237 178 601.0 0 178 238 186 601.0 0 186 239 210 601.0 0 210 240 144 601.0 0 144 241 162 601.0 0 162 242 179 601.0 0 179 243 220 601.0 0 220 244 152 601.0 0 152 245 334 601.0 0 334 246 445 601.0 0 445 247 411 601.0 0 411 248 150 601.0 0 150 249 127 601.0 0 127 250 126 601.0 0 126 251 176 601.0 0 176 252 190 601.0 0 190 253 174 601.0 0 174 254 179 601.0 0 179 255 241 601.0 0 241 256 256 601.0 0 256 257 194 601.0 0 194 258 151 601.0 0 151 259 169 601.0 0 169 260 204 601.0 0 204 261 187 601.0 0 187 262 172 601.0 0 172 263 160 601.0 0 160 264 212 601.0 0 212 265 203 601.0 0 203 266 217 601.0 0 217 267 1483 601.0 0 1483 268 1525 601.0 0 1525 269 451 601.0 0 451 270 212 601.0 0 212 271 162 601.0 0 162 272 149 601.0 0 149 273 153 601.0 0 153 274 145 601.0 0 145 275 224 601.0 0 224 276 200 601.0 0 200 277 700 601.0 0 700 278 280 601.0 0 280 279 197 601.0 0 197 280 240 601.0 0 240 281 208 601.0 0 208 282 217 601.0 0 217 283 165 601.0 0 165 284 125 601.0 0 125 285 118 601.0 0 118 286 184 601.0 0 184 287 164 601.0 0 164 288 187 601.0 0 187 289 195 601.0 0 195 290 139 601.0 0 139 291 180 601.0 0 180 292 164 601.0 0 164 293 215 601.0 0 215 294 252 601.0 0 252 295 186 601.0 0 186 296 144 601.0 0 144 297 175 601.0 0 175 298 354 601.0 0 354 299 277 601.0 0 277 300 158 601.0 0 158 301 152 601.0 0 152 Finished in 5.38 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 138,737 Reads with adapters: 488 (0.4%) Reads that were too short: 99 (0.1%) Reads written (passing filters): 389 (0.3%) Total basepairs processed: 24,359,059 bp Total written (filtered): 83,355 bp (0.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 488 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 20 89 0.0 4 80 5 2 1 1 21 39 0.0 4 20 11 7 1 22 52 0.0 4 39 7 4 1 1 23 3 0.0 4 0 0 0 1 2 24 5 0.0 4 0 0 0 1 4 25 2 0.0 4 0 0 0 1 1 31 3 0.0 4 0 0 0 0 3 34 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 44 2 0.0 4 0 0 0 0 2 46 2 0.0 4 0 0 0 0 2 47 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 49 9 0.0 4 0 0 0 2 7 50 2 0.0 4 0 0 0 0 2 51 121 0.0 4 0 0 0 1 120 54 2 0.0 4 0 0 0 0 2 56 1 0.0 4 0 0 0 0 1 59 2 0.0 4 0 0 0 0 2 63 2 0.0 4 0 0 0 1 1 64 1 0.0 4 0 0 0 0 1 70 2 0.0 4 0 0 0 0 2 73 3 0.0 4 0 0 0 0 3 74 2 0.0 4 0 0 0 0 2 75 4 0.0 4 0 0 0 0 4 76 5 0.0 4 0 0 0 0 5 77 5 0.0 4 0 0 0 0 5 80 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 98 2 0.0 4 0 0 0 0 2 99 2 0.0 4 0 0 0 0 2 100 2 0.0 4 0 0 0 0 2 104 3 0.0 4 0 0 0 0 3 106 1 0.0 4 0 0 1 107 2 0.0 4 0 0 0 0 2 110 30 0.0 4 0 0 0 0 30 113 2 0.0 4 0 0 0 0 2 115 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 119 2 0.0 4 0 0 0 0 2 122 3 0.0 4 0 0 0 0 3 123 2 0.0 4 0 0 0 0 2 124 3 0.0 4 0 0 0 0 3 125 1 0.0 4 0 0 0 0 1 128 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 134 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 0 1 136 4 0.0 4 0 0 0 0 4 138 1 0.0 4 0 0 0 1 139 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 150 2 0.0 4 0 0 0 0 2 153 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 157 2 0.0 4 0 0 0 0 2 159 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 167 3 0.0 4 0 0 0 0 3 171 2 0.0 4 0 0 0 0 2 177 2 0.0 4 0 0 0 0 2 178 1 0.0 4 0 0 0 0 1 182 2 0.0 4 0 0 0 0 2 187 1 0.0 4 0 0 0 0 1 188 2 0.0 4 0 0 0 0 2 193 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 208 6 0.0 4 0 0 0 1 5 213 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 4 0.0 4 0 0 0 0 4 239 1 0.0 4 0 0 0 0 1 240 1 0.0 4 0 0 0 0 1 243 1 0.0 4 0 0 0 0 1 248 1 0.0 4 0 0 0 1 267 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 389 Reads with adapters: 32 (8.2%) Reads written (passing filters): 389 (100.0%) Total basepairs processed: 83,355 bp Total written (filtered): 83,090 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 32 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 15.6% C: 15.6% G: 62.5% T: 6.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 6 6.1 0 6 4 4 1.5 0 4 9 1 0.0 0 0 1 10 10 0.0 1 1 9 11 10 0.0 1 1 9 12 1 0.0 1 0 1