This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 63.53 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 94,466 (3.8%) Reads that were too short: 47,048 (1.9%) Reads written (passing filters): 47,418 (1.9%) Total basepairs processed: 740,966,583 bp Total written (filtered): 10,984,969 bp (1.5%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 94466 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 220 601.0 0 220 7 383 601.0 0 383 8 10963 601.0 0 10963 9 8042 601.0 0 8042 10 111 601.0 0 111 11 99 601.0 0 99 12 115 601.0 0 115 13 112 601.0 0 112 14 143 601.0 0 143 15 121 601.0 0 121 16 135 601.0 0 135 17 110 601.0 0 110 18 103 601.0 0 103 19 128 601.0 0 128 20 106 601.0 0 106 21 102 601.0 0 102 22 110 601.0 0 110 23 120 601.0 0 120 24 97 601.0 0 97 25 127 601.0 0 127 26 132 601.0 0 132 27 123 601.0 0 123 28 111 601.0 0 111 29 145 601.0 0 145 30 107 601.0 0 107 31 100 601.0 0 100 32 120 601.0 0 120 33 90 601.0 0 90 34 113 601.0 0 113 35 100 601.0 0 100 36 95 601.0 0 95 37 116 601.0 0 116 38 103 601.0 0 103 39 96 601.0 0 96 40 121 601.0 0 121 41 95 601.0 0 95 42 108 601.0 0 108 43 115 601.0 0 115 44 171 601.0 0 171 45 153 601.0 0 153 46 120 601.0 0 120 47 127 601.0 0 127 48 123 601.0 0 123 49 102 601.0 0 102 50 105 601.0 0 105 51 105 601.0 0 105 52 108 601.0 0 108 53 145 601.0 0 145 54 165 601.0 0 165 55 123 601.0 0 123 56 170 601.0 0 170 57 102 601.0 0 102 58 185 601.0 0 185 59 220 601.0 0 220 60 212 601.0 0 212 61 202 601.0 0 202 62 120 601.0 0 120 63 96 601.0 0 96 64 105 601.0 0 105 65 120 601.0 0 120 66 104 601.0 0 104 67 156 601.0 0 156 68 108 601.0 0 108 69 121 601.0 0 121 70 147 601.0 0 147 71 259 601.0 0 259 72 149 601.0 0 149 73 155 601.0 0 155 74 111 601.0 0 111 75 127 601.0 0 127 76 224 601.0 0 224 77 185 601.0 0 185 78 210 601.0 0 210 79 136 601.0 0 136 80 122 601.0 0 122 81 168 601.0 0 168 82 129 601.0 0 129 83 119 601.0 0 119 84 110 601.0 0 110 85 141 601.0 0 141 86 90 601.0 0 90 87 94 601.0 0 94 88 113 601.0 0 113 89 108 601.0 0 108 90 121 601.0 0 121 91 130 601.0 0 130 92 151 601.0 0 151 93 158 601.0 0 158 94 137 601.0 0 137 95 141 601.0 0 141 96 121 601.0 0 121 97 109 601.0 0 109 98 116 601.0 0 116 99 107 601.0 0 107 100 135 601.0 0 135 101 107 601.0 0 107 102 117 601.0 0 117 103 135 601.0 0 135 104 100 601.0 0 100 105 126 601.0 0 126 106 131 601.0 0 131 107 127 601.0 0 127 108 132 601.0 0 132 109 146 601.0 0 146 110 220 601.0 0 220 111 205 601.0 0 205 112 224 601.0 0 224 113 149 601.0 0 149 114 134 601.0 0 134 115 128 601.0 0 128 116 133 601.0 0 133 117 150 601.0 0 150 118 111 601.0 0 111 119 122 601.0 0 122 120 138 601.0 0 138 121 106 601.0 0 106 122 113 601.0 0 113 123 134 601.0 0 134 124 106 601.0 0 106 125 137 601.0 0 137 126 149 601.0 0 149 127 124 601.0 0 124 128 161 601.0 0 161 129 161 601.0 0 161 130 244 601.0 0 244 131 265 601.0 0 265 132 307 601.0 0 307 133 169 601.0 0 169 134 197 601.0 0 197 135 159 601.0 0 159 136 161 601.0 0 161 137 142 601.0 0 142 138 259 601.0 0 259 139 237 601.0 0 237 140 205 601.0 0 205 141 145 601.0 0 145 142 200 601.0 0 200 143 173 601.0 0 173 144 207 601.0 0 207 145 219 601.0 0 219 146 170 601.0 0 170 147 170 601.0 0 170 148 212 601.0 0 212 149 140 601.0 0 140 150 153 601.0 0 153 151 190 601.0 0 190 152 143 601.0 0 143 153 139 601.0 0 139 154 140 601.0 0 140 155 131 601.0 0 131 156 152 601.0 0 152 157 155 601.0 0 155 158 151 601.0 0 151 159 132 601.0 0 132 160 102 601.0 0 102 161 139 601.0 0 139 162 116 601.0 0 116 163 126 601.0 0 126 164 128 601.0 0 128 165 153 601.0 0 153 166 151 601.0 0 151 167 114 601.0 0 114 168 131 601.0 0 131 169 104 601.0 0 104 170 130 601.0 0 130 171 117 601.0 0 117 172 109 601.0 0 109 173 113 601.0 0 113 174 133 601.0 0 133 175 108 601.0 0 108 176 114 601.0 0 114 177 112 601.0 0 112 178 114 601.0 0 114 179 170 601.0 0 170 180 177 601.0 0 177 181 139 601.0 0 139 182 116 601.0 0 116 183 128 601.0 0 128 184 107 601.0 0 107 185 109 601.0 0 109 186 136 601.0 0 136 187 113 601.0 0 113 188 126 601.0 0 126 189 109 601.0 0 109 190 144 601.0 0 144 191 143 601.0 0 143 192 175 601.0 0 175 193 192 601.0 0 192 194 148 601.0 0 148 195 158 601.0 0 158 196 123 601.0 0 123 197 306 601.0 0 306 198 468 601.0 0 468 199 760 601.0 0 760 200 106 601.0 0 106 201 130 601.0 0 130 202 96 601.0 0 96 203 87 601.0 0 87 204 169 601.0 0 169 205 131 601.0 0 131 206 117 601.0 0 117 207 88 601.0 0 88 208 99 601.0 0 99 209 113 601.0 0 113 210 136 601.0 0 136 211 118 601.0 0 118 212 128 601.0 0 128 213 128 601.0 0 128 214 109 601.0 0 109 215 135 601.0 0 135 216 125 601.0 0 125 217 117 601.0 0 117 218 119 601.0 0 119 219 99 601.0 0 99 220 113 601.0 0 113 221 124 601.0 0 124 222 146 601.0 0 146 223 286 601.0 0 286 224 154 601.0 0 154 225 374 601.0 0 374 226 142 601.0 0 142 227 156 601.0 0 156 228 133 601.0 0 133 229 95 601.0 0 95 230 125 601.0 0 125 231 118 601.0 0 118 232 127 601.0 0 127 233 191 601.0 0 191 234 162 601.0 0 162 235 130 601.0 0 130 236 152 601.0 0 152 237 139 601.0 0 139 238 209 601.0 0 209 239 197 601.0 0 197 240 318 601.0 0 318 241 338 601.0 0 338 242 229 601.0 0 229 243 143 601.0 0 143 244 135 601.0 0 135 245 247 601.0 0 247 246 261 601.0 0 261 247 196 601.0 0 196 248 134 601.0 0 134 249 117 601.0 0 117 250 118 601.0 0 118 251 132 601.0 0 132 252 144 601.0 0 144 253 125 601.0 0 125 254 180 601.0 0 180 255 204 601.0 0 204 256 273 601.0 0 273 257 214 601.0 0 214 258 213 601.0 0 213 259 168 601.0 0 168 260 175 601.0 0 175 261 211 601.0 0 211 262 257 601.0 0 257 263 225 601.0 0 225 264 212 601.0 0 212 265 1234 601.0 0 1234 266 1114 601.0 0 1114 267 1295 601.0 0 1295 268 310 601.0 0 310 269 110 601.0 0 110 270 105 601.0 0 105 271 166 601.0 0 166 272 250 601.0 0 250 273 255 601.0 0 255 274 182 601.0 0 182 275 146 601.0 0 146 276 124 601.0 0 124 277 153 601.0 0 153 278 172 601.0 0 172 279 196 601.0 0 196 280 204 601.0 0 204 281 223 601.0 0 223 282 197 601.0 0 197 283 176 601.0 0 176 284 239 601.0 0 239 285 245 601.0 0 245 286 294 601.0 0 294 287 254 601.0 0 254 288 343 601.0 0 343 289 268 601.0 0 268 290 268 601.0 0 268 291 392 601.0 0 392 292 425 601.0 0 425 293 428 601.0 0 428 294 396 601.0 0 396 295 390 601.0 0 390 296 440 601.0 0 440 297 1624 601.0 0 1624 298 6467 601.0 0 6467 299 7434 601.0 0 7434 300 6453 601.0 0 6453 301 3720 601.0 0 3720 Finished in 2.32 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 47,418 Reads with adapters: 8,548 (18.0%) Reads that were too short: 23 (0.0%) Reads written (passing filters): 8,525 (18.0%) Total basepairs processed: 10,984,969 bp Total written (filtered): 2,316,317 bp (21.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8548 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 8 0.0 3 0 0 5 2 1 19 147 0.0 3 0 128 10 7 2 20 8280 0.0 4 7661 498 83 28 10 21 29 0.0 4 4 16 7 1 1 22 2 0.0 4 1 0 1 23 31 0.0 4 1 0 0 16 14 24 1 0.0 4 1 40 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 101 2 0.0 4 0 0 0 0 2 103 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 21 0.0 4 0 0 0 0 21 129 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 1 152 1 0.0 4 0 0 0 0 1 185 2 0.0 4 0 0 1 1 186 2 0.0 4 1 0 1 187 1 0.0 4 0 0 0 1 192 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.32 s (38 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 8,525 Reads with adapters: 2,317 (27.2%) Reads written (passing filters): 8,525 (100.0%) Total basepairs processed: 2,316,317 bp Total written (filtered): 2,293,545 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2317 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 9.3% G: 86.4% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 133.2 0 8 4 2 33.3 0 2 5 1 8.3 0 1 6 2 2.1 0 2 7 1 0.5 0 1 8 3 0.1 0 3 9 1019 0.0 0 93 926 10 933 0.0 1 64 869 11 327 0.0 1 13 314 12 11 0.0 1 0 11 13 8 0.0 1 0 8 150 1 0.0 1 1 208 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.