This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 62.68 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 104,233 (4.2%) Reads that were too short: 61,498 (2.5%) Reads written (passing filters): 42,735 (1.7%) Total basepairs processed: 740,966,583 bp Total written (filtered): 10,246,693 bp (1.4%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 104233 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 158 601.0 0 158 7 312 601.0 0 312 8 9575 601.0 0 9575 9 10849 601.0 0 10849 10 66 601.0 0 66 11 73 601.0 0 73 12 105 601.0 0 105 13 84 601.0 0 84 14 92 601.0 0 92 15 108 601.0 0 108 16 112 601.0 0 112 17 84 601.0 0 84 18 97 601.0 0 97 19 90 601.0 0 90 20 87 601.0 0 87 21 98 601.0 0 98 22 91 601.0 0 91 23 106 601.0 0 106 24 109 601.0 0 109 25 99 601.0 0 99 26 93 601.0 0 93 27 90 601.0 0 90 28 74 601.0 0 74 29 100 601.0 0 100 30 72 601.0 0 72 31 67 601.0 0 67 32 99 601.0 0 99 33 75 601.0 0 75 34 104 601.0 0 104 35 81 601.0 0 81 36 75 601.0 0 75 37 101 601.0 0 101 38 83 601.0 0 83 39 69 601.0 0 69 40 92 601.0 0 92 41 96 601.0 0 96 42 102 601.0 0 102 43 78 601.0 0 78 44 115 601.0 0 115 45 108 601.0 0 108 46 100 601.0 0 100 47 80 601.0 0 80 48 115 601.0 0 115 49 88 601.0 0 88 50 87 601.0 0 87 51 80 601.0 0 80 52 110 601.0 0 110 53 83 601.0 0 83 54 122 601.0 0 122 55 103 601.0 0 103 56 130 601.0 0 130 57 72 601.0 0 72 58 187 601.0 0 187 59 200 601.0 0 200 60 235 601.0 0 235 61 233 601.0 0 233 62 88 601.0 0 88 63 103 601.0 0 103 64 104 601.0 0 104 65 99 601.0 0 99 66 84 601.0 0 84 67 107 601.0 0 107 68 105 601.0 0 105 69 111 601.0 0 111 70 130 601.0 0 130 71 360 601.0 0 360 72 125 601.0 0 125 73 111 601.0 0 111 74 83 601.0 0 83 75 94 601.0 0 94 76 115 601.0 0 115 77 114 601.0 0 114 78 148 601.0 0 148 79 101 601.0 0 101 80 115 601.0 0 115 81 151 601.0 0 151 82 102 601.0 0 102 83 90 601.0 0 90 84 97 601.0 0 97 85 106 601.0 0 106 86 87 601.0 0 87 87 112 601.0 0 112 88 78 601.0 0 78 89 105 601.0 0 105 90 105 601.0 0 105 91 133 601.0 0 133 92 109 601.0 0 109 93 107 601.0 0 107 94 105 601.0 0 105 95 89 601.0 0 89 96 87 601.0 0 87 97 84 601.0 0 84 98 97 601.0 0 97 99 88 601.0 0 88 100 79 601.0 0 79 101 86 601.0 0 86 102 111 601.0 0 111 103 110 601.0 0 110 104 98 601.0 0 98 105 94 601.0 0 94 106 106 601.0 0 106 107 80 601.0 0 80 108 85 601.0 0 85 109 124 601.0 0 124 110 112 601.0 0 112 111 130 601.0 0 130 112 122 601.0 0 122 113 96 601.0 0 96 114 115 601.0 0 115 115 80 601.0 0 80 116 116 601.0 0 116 117 89 601.0 0 89 118 104 601.0 0 104 119 80 601.0 0 80 120 111 601.0 0 111 121 94 601.0 0 94 122 94 601.0 0 94 123 98 601.0 0 98 124 81 601.0 0 81 125 114 601.0 0 114 126 89 601.0 0 89 127 77 601.0 0 77 128 87 601.0 0 87 129 113 601.0 0 113 130 164 601.0 0 164 131 157 601.0 0 157 132 185 601.0 0 185 133 114 601.0 0 114 134 143 601.0 0 143 135 114 601.0 0 114 136 118 601.0 0 118 137 134 601.0 0 134 138 205 601.0 0 205 139 161 601.0 0 161 140 155 601.0 0 155 141 117 601.0 0 117 142 200 601.0 0 200 143 134 601.0 0 134 144 175 601.0 0 175 145 142 601.0 0 142 146 123 601.0 0 123 147 122 601.0 0 122 148 218 601.0 0 218 149 86 601.0 0 86 150 99 601.0 0 99 151 109 601.0 0 109 152 111 601.0 0 111 153 103 601.0 0 103 154 112 601.0 0 112 155 104 601.0 0 104 156 108 601.0 0 108 157 100 601.0 0 100 158 138 601.0 0 138 159 105 601.0 0 105 160 93 601.0 0 93 161 121 601.0 0 121 162 79 601.0 0 79 163 72 601.0 0 72 164 84 601.0 0 84 165 94 601.0 0 94 166 93 601.0 0 93 167 74 601.0 0 74 168 88 601.0 0 88 169 82 601.0 0 82 170 70 601.0 0 70 171 99 601.0 0 99 172 76 601.0 0 76 173 82 601.0 0 82 174 89 601.0 0 89 175 72 601.0 0 72 176 86 601.0 0 86 177 92 601.0 0 92 178 81 601.0 0 81 179 147 601.0 0 147 180 126 601.0 0 126 181 95 601.0 0 95 182 96 601.0 0 96 183 84 601.0 0 84 184 100 601.0 0 100 185 89 601.0 0 89 186 92 601.0 0 92 187 100 601.0 0 100 188 95 601.0 0 95 189 104 601.0 0 104 190 101 601.0 0 101 191 101 601.0 0 101 192 187 601.0 0 187 193 206 601.0 0 206 194 116 601.0 0 116 195 106 601.0 0 106 196 87 601.0 0 87 197 250 601.0 0 250 198 391 601.0 0 391 199 789 601.0 0 789 200 106 601.0 0 106 201 86 601.0 0 86 202 87 601.0 0 87 203 86 601.0 0 86 204 229 601.0 0 229 205 112 601.0 0 112 206 103 601.0 0 103 207 69 601.0 0 69 208 63 601.0 0 63 209 76 601.0 0 76 210 92 601.0 0 92 211 69 601.0 0 69 212 93 601.0 0 93 213 80 601.0 0 80 214 86 601.0 0 86 215 93 601.0 0 93 216 88 601.0 0 88 217 104 601.0 0 104 218 82 601.0 0 82 219 90 601.0 0 90 220 89 601.0 0 89 221 107 601.0 0 107 222 165 601.0 0 165 223 298 601.0 0 298 224 139 601.0 0 139 225 518 601.0 0 518 226 110 601.0 0 110 227 79 601.0 0 79 228 82 601.0 0 82 229 90 601.0 0 90 230 87 601.0 0 87 231 105 601.0 0 105 232 109 601.0 0 109 233 109 601.0 0 109 234 99 601.0 0 99 235 80 601.0 0 80 236 99 601.0 0 99 237 91 601.0 0 91 238 125 601.0 0 125 239 106 601.0 0 106 240 138 601.0 0 138 241 144 601.0 0 144 242 126 601.0 0 126 243 92 601.0 0 92 244 97 601.0 0 97 245 156 601.0 0 156 246 143 601.0 0 143 247 108 601.0 0 108 248 83 601.0 0 83 249 91 601.0 0 91 250 66 601.0 0 66 251 108 601.0 0 108 252 94 601.0 0 94 253 98 601.0 0 98 254 105 601.0 0 105 255 138 601.0 0 138 256 151 601.0 0 151 257 125 601.0 0 125 258 108 601.0 0 108 259 134 601.0 0 134 260 130 601.0 0 130 261 137 601.0 0 137 262 126 601.0 0 126 263 126 601.0 0 126 264 118 601.0 0 118 265 375 601.0 0 375 266 464 601.0 0 464 267 374 601.0 0 374 268 144 601.0 0 144 269 69 601.0 0 69 270 79 601.0 0 79 271 94 601.0 0 94 272 135 601.0 0 135 273 175 601.0 0 175 274 174 601.0 0 174 275 119 601.0 0 119 276 92 601.0 0 92 277 137 601.0 0 137 278 126 601.0 0 126 279 158 601.0 0 158 280 205 601.0 0 205 281 222 601.0 0 222 282 235 601.0 0 235 283 187 601.0 0 187 284 275 601.0 0 275 285 315 601.0 0 315 286 384 601.0 0 384 287 250 601.0 0 250 288 411 601.0 0 411 289 350 601.0 0 350 290 389 601.0 0 389 291 695 601.0 0 695 292 656 601.0 0 656 293 591 601.0 0 591 294 581 601.0 0 581 295 555 601.0 0 555 296 654 601.0 0 654 297 2118 601.0 0 2118 298 10994 601.0 0 10994 299 12205 601.0 0 12205 300 11985 601.0 0 11985 301 7195 601.0 0 7195 Finished in 1.89 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 42,735 Reads with adapters: 9,137 (21.4%) Reads that were too short: 61 (0.1%) Reads written (passing filters): 9,076 (21.2%) Total basepairs processed: 10,246,693 bp Total written (filtered): 2,503,332 bp (24.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9137 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 4 0.0 3 0 0 4 16 55 0.0 3 0 53 2 17 8955 0.0 3 8793 158 3 1 18 31 0.0 3 1 29 1 19 7 0.0 3 0 0 6 1 20 12 0.0 3 0 0 0 12 30 1 0.0 3 1 39 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 181 1 0.0 3 1 182 1 0.0 3 1 184 2 0.0 3 1 1 189 40 0.0 3 0 0 1 39 207 1 0.0 3 0 0 0 1 217 9 0.0 3 0 0 0 9 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.32 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 9,076 Reads with adapters: 28 (0.3%) Reads written (passing filters): 9,076 (100.0%) Total basepairs processed: 2,503,332 bp Total written (filtered): 2,503,241 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 28 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 75.0% C: 3.6% G: 17.9% T: 3.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 22 141.8 0 22 4 5 35.5 0 5 5 1 8.9 0 1