This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.90 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 139,530 (5.7%) Reads that were too short: 36,304 (1.5%) Reads written (passing filters): 103,226 (4.2%) Total basepairs processed: 740,966,583 bp Total written (filtered): 26,987,466 bp (3.6%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 139530 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1638 601.0 0 1638 7 42365 601.0 0 42365 8 29094 601.0 0 29094 9 91 601.0 0 91 10 80 601.0 0 80 11 80 601.0 0 80 12 105 601.0 0 105 13 68 601.0 0 68 14 85 601.0 0 85 15 63 601.0 0 63 16 70 601.0 0 70 17 79 601.0 0 79 18 73 601.0 0 73 19 76 601.0 0 76 20 64 601.0 0 64 21 81 601.0 0 81 22 74 601.0 0 74 23 75 601.0 0 75 24 100 601.0 0 100 25 76 601.0 0 76 26 79 601.0 0 79 27 68 601.0 0 68 28 76 601.0 0 76 29 86 601.0 0 86 30 87 601.0 0 87 31 76 601.0 0 76 32 75 601.0 0 75 33 69 601.0 0 69 34 72 601.0 0 72 35 99 601.0 0 99 36 102 601.0 0 102 37 103 601.0 0 103 38 82 601.0 0 82 39 113 601.0 0 113 40 116 601.0 0 116 41 125 601.0 0 125 42 102 601.0 0 102 43 100 601.0 0 100 44 184 601.0 0 184 45 67 601.0 0 67 46 96 601.0 0 96 47 96 601.0 0 96 48 72 601.0 0 72 49 112 601.0 0 112 50 78 601.0 0 78 51 109 601.0 0 109 52 100 601.0 0 100 53 111 601.0 0 111 54 117 601.0 0 117 55 114 601.0 0 114 56 110 601.0 0 110 57 95 601.0 0 95 58 112 601.0 0 112 59 114 601.0 0 114 60 101 601.0 0 101 61 65 601.0 0 65 62 114 601.0 0 114 63 139 601.0 0 139 64 1460 601.0 0 1460 65 792 601.0 0 792 66 986 601.0 0 986 67 163 601.0 0 163 68 126 601.0 0 126 69 549 601.0 0 549 70 247 601.0 0 247 71 86 601.0 0 86 72 58 601.0 0 58 73 66 601.0 0 66 74 92 601.0 0 92 75 107 601.0 0 107 76 86 601.0 0 86 77 64 601.0 0 64 78 49 601.0 0 49 79 109 601.0 0 109 80 94 601.0 0 94 81 96 601.0 0 96 82 63 601.0 0 63 83 80 601.0 0 80 84 70 601.0 0 70 85 77 601.0 0 77 86 71 601.0 0 71 87 69 601.0 0 69 88 73 601.0 0 73 89 100 601.0 0 100 90 116 601.0 0 116 91 121 601.0 0 121 92 330 601.0 0 330 93 285 601.0 0 285 94 250 601.0 0 250 95 86 601.0 0 86 96 72 601.0 0 72 97 59 601.0 0 59 98 50 601.0 0 50 99 157 601.0 0 157 100 112 601.0 0 112 101 157 601.0 0 157 102 113 601.0 0 113 103 88 601.0 0 88 104 89 601.0 0 89 105 71 601.0 0 71 106 69 601.0 0 69 107 64 601.0 0 64 108 87 601.0 0 87 109 64 601.0 0 64 110 95 601.0 0 95 111 101 601.0 0 101 112 160 601.0 0 160 113 94 601.0 0 94 114 89 601.0 0 89 115 82 601.0 0 82 116 77 601.0 0 77 117 95 601.0 0 95 118 96 601.0 0 96 119 80 601.0 0 80 120 86 601.0 0 86 121 94 601.0 0 94 122 95 601.0 0 95 123 116 601.0 0 116 124 90 601.0 0 90 125 109 601.0 0 109 126 98 601.0 0 98 127 94 601.0 0 94 128 89 601.0 0 89 129 82 601.0 0 82 130 274 601.0 0 274 131 358 601.0 0 358 132 183 601.0 0 183 133 162 601.0 0 162 134 109 601.0 0 109 135 199 601.0 0 199 136 268 601.0 0 268 137 261 601.0 0 261 138 111 601.0 0 111 139 100 601.0 0 100 140 86 601.0 0 86 141 90 601.0 0 90 142 99 601.0 0 99 143 92 601.0 0 92 144 94 601.0 0 94 145 104 601.0 0 104 146 76 601.0 0 76 147 161 601.0 0 161 148 222 601.0 0 222 149 260 601.0 0 260 150 143 601.0 0 143 151 99 601.0 0 99 152 136 601.0 0 136 153 112 601.0 0 112 154 117 601.0 0 117 155 220 601.0 0 220 156 441 601.0 0 441 157 444 601.0 0 444 158 268 601.0 0 268 159 132 601.0 0 132 160 223 601.0 0 223 161 171 601.0 0 171 162 149 601.0 0 149 163 126 601.0 0 126 164 105 601.0 0 105 165 102 601.0 0 102 166 193 601.0 0 193 167 422 601.0 0 422 168 298 601.0 0 298 169 595 601.0 0 595 170 95 601.0 0 95 171 236 601.0 0 236 172 335 601.0 0 335 173 171 601.0 0 171 174 103 601.0 0 103 175 303 601.0 0 303 176 465 601.0 0 465 177 751 601.0 0 751 178 455 601.0 0 455 179 105 601.0 0 105 180 110 601.0 0 110 181 155 601.0 0 155 182 117 601.0 0 117 183 132 601.0 0 132 184 104 601.0 0 104 185 152 601.0 0 152 186 213 601.0 0 213 187 219 601.0 0 219 188 462 601.0 0 462 189 243 601.0 0 243 190 570 601.0 0 570 191 104 601.0 0 104 192 119 601.0 0 119 193 100 601.0 0 100 194 83 601.0 0 83 195 103 601.0 0 103 196 177 601.0 0 177 197 191 601.0 0 191 198 180 601.0 0 180 199 124 601.0 0 124 200 155 601.0 0 155 201 221 601.0 0 221 202 183 601.0 0 183 203 167 601.0 0 167 204 157 601.0 0 157 205 121 601.0 0 121 206 125 601.0 0 125 207 143 601.0 0 143 208 135 601.0 0 135 209 120 601.0 0 120 210 182 601.0 0 182 211 169 601.0 0 169 212 176 601.0 0 176 213 155 601.0 0 155 214 142 601.0 0 142 215 213 601.0 0 213 216 501 601.0 0 501 217 355 601.0 0 355 218 328 601.0 0 328 219 219 601.0 0 219 220 229 601.0 0 229 221 1111 601.0 0 1111 222 1363 601.0 0 1363 223 1034 601.0 0 1034 224 1126 601.0 0 1126 225 244 601.0 0 244 226 251 601.0 0 251 227 335 601.0 0 335 228 341 601.0 0 341 229 202 601.0 0 202 230 114 601.0 0 114 231 111 601.0 0 111 232 173 601.0 0 173 233 164 601.0 0 164 234 218 601.0 0 218 235 141 601.0 0 141 236 133 601.0 0 133 237 214 601.0 0 214 238 195 601.0 0 195 239 335 601.0 0 335 240 205 601.0 0 205 241 1026 601.0 0 1026 242 972 601.0 0 972 243 1123 601.0 0 1123 244 688 601.0 0 688 245 361 601.0 0 361 246 386 601.0 0 386 247 230 601.0 0 230 248 246 601.0 0 246 249 161 601.0 0 161 250 218 601.0 0 218 251 191 601.0 0 191 252 314 601.0 0 314 253 371 601.0 0 371 254 510 601.0 0 510 255 325 601.0 0 325 256 410 601.0 0 410 257 237 601.0 0 237 258 153 601.0 0 153 259 138 601.0 0 138 260 143 601.0 0 143 261 166 601.0 0 166 262 163 601.0 0 163 263 233 601.0 0 233 264 438 601.0 0 438 265 355 601.0 0 355 266 326 601.0 0 326 267 166 601.0 0 166 268 488 601.0 0 488 269 633 601.0 0 633 270 1512 601.0 0 1512 271 926 601.0 0 926 272 568 601.0 0 568 273 418 601.0 0 418 274 272 601.0 0 272 275 263 601.0 0 263 276 269 601.0 0 269 277 218 601.0 0 218 278 198 601.0 0 198 279 206 601.0 0 206 280 153 601.0 0 153 281 190 601.0 0 190 282 344 601.0 0 344 283 405 601.0 0 405 284 408 601.0 0 408 285 287 601.0 0 287 286 202 601.0 0 202 287 324 601.0 0 324 288 164 601.0 0 164 289 225 601.0 0 225 290 434 601.0 0 434 291 931 601.0 0 931 292 620 601.0 0 620 293 361 601.0 0 361 294 243 601.0 0 243 295 210 601.0 0 210 296 210 601.0 0 210 297 230 601.0 0 230 298 283 601.0 0 283 299 710 601.0 0 710 300 814 601.0 0 814 301 705 601.0 0 705 Finished in 5.06 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 103,226 Reads with adapters: 29,770 (28.8%) Reads that were too short: 33 (0.0%) Reads written (passing filters): 29,737 (28.8%) Total basepairs processed: 26,987,466 bp Total written (filtered): 8,115,097 bp (30.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 29770 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 0 2 17 11 0.0 3 0 0 0 5 6 18 28 0.0 3 0 0 14 12 2 19 381 0.0 3 0 261 57 43 20 20 28911 0.0 4 26169 2093 470 143 36 21 116 0.0 4 14 84 5 4 9 22 9 0.0 4 0 0 7 1 1 23 239 0.0 4 27 3 2 130 77 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 1 32 1 0.0 4 0 0 1 35 12 0.0 4 0 0 0 0 12 37 1 0.0 4 1 51 1 0.0 4 0 0 0 0 1 56 1 0.0 4 1 57 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 99 2 0.0 4 0 0 0 0 2 108 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 127 10 0.0 4 0 0 0 0 10 134 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 153 7 0.0 4 0 0 0 1 6 168 1 0.0 4 0 0 0 0 1 207 2 0.0 4 0 0 0 0 2 208 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 226 1 0.0 4 1 227 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 233 5 0.0 4 0 0 0 0 5 234 2 0.0 4 0 0 0 0 2 235 2 0.0 4 0 0 0 0 2 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.06 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 29,737 Reads with adapters: 5,507 (18.5%) Reads written (passing filters): 29,737 (100.0%) Total basepairs processed: 8,115,097 bp Total written (filtered): 8,009,273 bp (98.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5507 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.9% C: 10.2% G: 79.6% T: 2.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 79 464.6 0 79 4 11 116.2 0 11 5 1 29.0 0 1 9 19 0.1 0 1 18 10 205 0.0 1 19 186 11 4818 0.0 1 190 4628 12 23 0.0 1 0 23 14 9 0.0 1 0 9 15 2 0.0 1 0 2 19 62 0.0 1 42 20 20 10 0.0 1 7 3 22 3 0.0 1 3 23 28 0.0 1 17 11 24 3 0.0 1 3 25 1 0.0 1 0 1 90 1 0.0 1 1 91 1 0.0 1 1 115 7 0.0 1 6 1 151 3 0.0 1 3 197 1 0.0 1 1 208 2 0.0 1 2 209 208 0.0 1 165 43 210 4 0.0 1 4 211 2 0.0 1 2 229 4 0.0 1 3 1