This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.86 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 102,866 (4.2%) Reads that were too short: 12,106 (0.5%) Reads written (passing filters): 90,760 (3.7%) Total basepairs processed: 740,966,583 bp Total written (filtered): 25,206,278 bp (3.4%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 102866 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 917 601.0 0 917 7 32033 601.0 0 32033 8 43579 601.0 0 43579 9 78 601.0 0 78 10 41 601.0 0 41 11 49 601.0 0 49 12 44 601.0 0 44 13 48 601.0 0 48 14 60 601.0 0 60 15 42 601.0 0 42 16 53 601.0 0 53 17 51 601.0 0 51 18 46 601.0 0 46 19 54 601.0 0 54 20 39 601.0 0 39 21 35 601.0 0 35 22 56 601.0 0 56 23 45 601.0 0 45 24 51 601.0 0 51 25 64 601.0 0 64 26 45 601.0 0 45 27 46 601.0 0 46 28 63 601.0 0 63 29 66 601.0 0 66 30 54 601.0 0 54 31 66 601.0 0 66 32 107 601.0 0 107 33 41 601.0 0 41 34 36 601.0 0 36 35 86 601.0 0 86 36 53 601.0 0 53 37 68 601.0 0 68 38 40 601.0 0 40 39 66 601.0 0 66 40 52 601.0 0 52 41 60 601.0 0 60 42 62 601.0 0 62 43 91 601.0 0 91 44 185 601.0 0 185 45 39 601.0 0 39 46 56 601.0 0 56 47 78 601.0 0 78 48 49 601.0 0 49 49 64 601.0 0 64 50 58 601.0 0 58 51 42 601.0 0 42 52 53 601.0 0 53 53 51 601.0 0 51 54 64 601.0 0 64 55 48 601.0 0 48 56 32 601.0 0 32 57 50 601.0 0 50 58 40 601.0 0 40 59 47 601.0 0 47 60 42 601.0 0 42 61 41 601.0 0 41 62 83 601.0 0 83 63 88 601.0 0 88 64 594 601.0 0 594 65 211 601.0 0 211 66 466 601.0 0 466 67 157 601.0 0 157 68 63 601.0 0 63 69 594 601.0 0 594 70 67 601.0 0 67 71 40 601.0 0 40 72 39 601.0 0 39 73 49 601.0 0 49 74 40 601.0 0 40 75 46 601.0 0 46 76 35 601.0 0 35 77 45 601.0 0 45 78 26 601.0 0 26 79 44 601.0 0 44 80 58 601.0 0 58 81 59 601.0 0 59 82 35 601.0 0 35 83 41 601.0 0 41 84 53 601.0 0 53 85 46 601.0 0 46 86 53 601.0 0 53 87 39 601.0 0 39 88 44 601.0 0 44 89 38 601.0 0 38 90 72 601.0 0 72 91 63 601.0 0 63 92 207 601.0 0 207 93 157 601.0 0 157 94 179 601.0 0 179 95 52 601.0 0 52 96 37 601.0 0 37 97 42 601.0 0 42 98 38 601.0 0 38 99 42 601.0 0 42 100 31 601.0 0 31 101 32 601.0 0 32 102 47 601.0 0 47 103 47 601.0 0 47 104 49 601.0 0 49 105 40 601.0 0 40 106 35 601.0 0 35 107 38 601.0 0 38 108 47 601.0 0 47 109 59 601.0 0 59 110 39 601.0 0 39 111 71 601.0 0 71 112 49 601.0 0 49 113 34 601.0 0 34 114 45 601.0 0 45 115 49 601.0 0 49 116 39 601.0 0 39 117 69 601.0 0 69 118 57 601.0 0 57 119 43 601.0 0 43 120 66 601.0 0 66 121 60 601.0 0 60 122 53 601.0 0 53 123 48 601.0 0 48 124 53 601.0 0 53 125 54 601.0 0 54 126 57 601.0 0 57 127 45 601.0 0 45 128 57 601.0 0 57 129 48 601.0 0 48 130 81 601.0 0 81 131 73 601.0 0 73 132 66 601.0 0 66 133 64 601.0 0 64 134 60 601.0 0 60 135 55 601.0 0 55 136 78 601.0 0 78 137 100 601.0 0 100 138 63 601.0 0 63 139 46 601.0 0 46 140 50 601.0 0 50 141 55 601.0 0 55 142 55 601.0 0 55 143 34 601.0 0 34 144 61 601.0 0 61 145 39 601.0 0 39 146 41 601.0 0 41 147 59 601.0 0 59 148 100 601.0 0 100 149 90 601.0 0 90 150 91 601.0 0 91 151 60 601.0 0 60 152 59 601.0 0 59 153 95 601.0 0 95 154 52 601.0 0 52 155 80 601.0 0 80 156 155 601.0 0 155 157 123 601.0 0 123 158 87 601.0 0 87 159 69 601.0 0 69 160 103 601.0 0 103 161 69 601.0 0 69 162 57 601.0 0 57 163 64 601.0 0 64 164 59 601.0 0 59 165 45 601.0 0 45 166 77 601.0 0 77 167 188 601.0 0 188 168 98 601.0 0 98 169 168 601.0 0 168 170 52 601.0 0 52 171 101 601.0 0 101 172 128 601.0 0 128 173 82 601.0 0 82 174 54 601.0 0 54 175 141 601.0 0 141 176 142 601.0 0 142 177 146 601.0 0 146 178 78 601.0 0 78 179 50 601.0 0 50 180 61 601.0 0 61 181 63 601.0 0 63 182 59 601.0 0 59 183 65 601.0 0 65 184 45 601.0 0 45 185 63 601.0 0 63 186 78 601.0 0 78 187 54 601.0 0 54 188 141 601.0 0 141 189 93 601.0 0 93 190 253 601.0 0 253 191 89 601.0 0 89 192 55 601.0 0 55 193 67 601.0 0 67 194 48 601.0 0 48 195 70 601.0 0 70 196 66 601.0 0 66 197 70 601.0 0 70 198 65 601.0 0 65 199 63 601.0 0 63 200 72 601.0 0 72 201 48 601.0 0 48 202 73 601.0 0 73 203 73 601.0 0 73 204 90 601.0 0 90 205 102 601.0 0 102 206 88 601.0 0 88 207 95 601.0 0 95 208 70 601.0 0 70 209 61 601.0 0 61 210 76 601.0 0 76 211 77 601.0 0 77 212 104 601.0 0 104 213 107 601.0 0 107 214 80 601.0 0 80 215 84 601.0 0 84 216 100 601.0 0 100 217 93 601.0 0 93 218 73 601.0 0 73 219 80 601.0 0 80 220 98 601.0 0 98 221 213 601.0 0 213 222 235 601.0 0 235 223 175 601.0 0 175 224 162 601.0 0 162 225 117 601.0 0 117 226 94 601.0 0 94 227 129 601.0 0 129 228 124 601.0 0 124 229 84 601.0 0 84 230 53 601.0 0 53 231 67 601.0 0 67 232 69 601.0 0 69 233 74 601.0 0 74 234 99 601.0 0 99 235 89 601.0 0 89 236 89 601.0 0 89 237 75 601.0 0 75 238 132 601.0 0 132 239 106 601.0 0 106 240 97 601.0 0 97 241 297 601.0 0 297 242 209 601.0 0 209 243 255 601.0 0 255 244 212 601.0 0 212 245 142 601.0 0 142 246 93 601.0 0 93 247 106 601.0 0 106 248 119 601.0 0 119 249 77 601.0 0 77 250 99 601.0 0 99 251 114 601.0 0 114 252 109 601.0 0 109 253 146 601.0 0 146 254 171 601.0 0 171 255 106 601.0 0 106 256 110 601.0 0 110 257 81 601.0 0 81 258 69 601.0 0 69 259 77 601.0 0 77 260 73 601.0 0 73 261 59 601.0 0 59 262 67 601.0 0 67 263 94 601.0 0 94 264 102 601.0 0 102 265 97 601.0 0 97 266 111 601.0 0 111 267 82 601.0 0 82 268 212 601.0 0 212 269 223 601.0 0 223 270 260 601.0 0 260 271 218 601.0 0 218 272 144 601.0 0 144 273 162 601.0 0 162 274 107 601.0 0 107 275 113 601.0 0 113 276 111 601.0 0 111 277 152 601.0 0 152 278 130 601.0 0 130 279 160 601.0 0 160 280 74 601.0 0 74 281 83 601.0 0 83 282 102 601.0 0 102 283 130 601.0 0 130 284 124 601.0 0 124 285 86 601.0 0 86 286 91 601.0 0 91 287 104 601.0 0 104 288 91 601.0 0 91 289 106 601.0 0 106 290 120 601.0 0 120 291 204 601.0 0 204 292 202 601.0 0 202 293 131 601.0 0 131 294 86 601.0 0 86 295 85 601.0 0 85 296 104 601.0 0 104 297 147 601.0 0 147 298 172 601.0 0 172 299 181 601.0 0 181 300 263 601.0 0 263 301 244 601.0 0 244 Finished in 4.25 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 90,760 Reads with adapters: 31,732 (35.0%) Reads that were too short: 29 (0.0%) Reads written (passing filters): 31,703 (34.9%) Total basepairs processed: 25,206,278 bp Total written (filtered): 8,777,686 bp (34.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 31732 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 5 0.0 3 0 0 5 16 169 0.0 3 0 166 3 17 31343 0.0 3 30703 621 19 18 64 0.0 3 2 58 4 19 8 0.0 3 2 0 6 20 87 0.0 3 1 0 0 86 21 1 0.0 3 1 22 2 0.0 3 1 0 0 1 26 1 0.0 3 1 29 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 151 5 0.0 3 0 1 0 4 152 1 0.0 3 1 153 1 0.0 3 1 157 1 0.0 3 0 0 0 1 160 13 0.0 3 0 0 0 13 161 3 0.0 3 0 0 0 3 168 1 0.0 3 0 0 0 1 171 1 0.0 3 1 173 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 6 0.0 3 0 0 0 6 218 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.11 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 31,703 Reads with adapters: 37 (0.1%) Reads written (passing filters): 31,703 (100.0%) Total basepairs processed: 8,777,686 bp Total written (filtered): 8,777,569 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 37 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 10.8% C: 10.8% G: 62.2% T: 16.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 33 495.4 0 33 4 3 123.8 0 3 6 1 7.7 0 1