This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 61.30 s (25 us/read; 2.41 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 238,368 (9.7%) Reads that were too short: 198,493 (8.1%) Reads written (passing filters): 39,875 (1.6%) Total basepairs processed: 740,966,583 bp Total written (filtered): 8,801,905 bp (1.2%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 238368 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 246 601.0 0 246 7 6289 601.0 0 6289 8 332 601.0 0 332 9 6877 601.0 0 6877 10 135 601.0 0 135 11 112 601.0 0 112 12 90 601.0 0 90 13 112 601.0 0 112 14 125 601.0 0 125 15 102 601.0 0 102 16 110 601.0 0 110 17 86 601.0 0 86 18 103 601.0 0 103 19 103 601.0 0 103 20 89 601.0 0 89 21 100 601.0 0 100 22 89 601.0 0 89 23 106 601.0 0 106 24 99 601.0 0 99 25 102 601.0 0 102 26 113 601.0 0 113 27 98 601.0 0 98 28 120 601.0 0 120 29 179 601.0 0 179 30 146 601.0 0 146 31 125 601.0 0 125 32 115 601.0 0 115 33 91 601.0 0 91 34 101 601.0 0 101 35 110 601.0 0 110 36 139 601.0 0 139 37 119 601.0 0 119 38 161 601.0 0 161 39 77 601.0 0 77 40 82 601.0 0 82 41 81 601.0 0 81 42 73 601.0 0 73 43 104 601.0 0 104 44 90 601.0 0 90 45 104 601.0 0 104 46 100 601.0 0 100 47 79 601.0 0 79 48 68 601.0 0 68 49 83 601.0 0 83 50 76 601.0 0 76 51 75 601.0 0 75 52 65 601.0 0 65 53 63 601.0 0 63 54 68 601.0 0 68 55 68 601.0 0 68 56 89 601.0 0 89 57 79 601.0 0 79 58 62 601.0 0 62 59 88 601.0 0 88 60 91 601.0 0 91 61 79 601.0 0 79 62 56 601.0 0 56 63 63 601.0 0 63 64 56 601.0 0 56 65 78 601.0 0 78 66 94 601.0 0 94 67 84 601.0 0 84 68 86 601.0 0 86 69 157 601.0 0 157 70 93 601.0 0 93 71 118 601.0 0 118 72 92 601.0 0 92 73 105 601.0 0 105 74 78 601.0 0 78 75 103 601.0 0 103 76 169 601.0 0 169 77 68 601.0 0 68 78 74 601.0 0 74 79 92 601.0 0 92 80 97 601.0 0 97 81 87 601.0 0 87 82 94 601.0 0 94 83 138 601.0 0 138 84 85 601.0 0 85 85 78 601.0 0 78 86 104 601.0 0 104 87 86 601.0 0 86 88 72 601.0 0 72 89 83 601.0 0 83 90 72 601.0 0 72 91 68 601.0 0 68 92 85 601.0 0 85 93 78 601.0 0 78 94 80 601.0 0 80 95 88 601.0 0 88 96 85 601.0 0 85 97 81 601.0 0 81 98 97 601.0 0 97 99 103 601.0 0 103 100 95 601.0 0 95 101 82 601.0 0 82 102 108 601.0 0 108 103 133 601.0 0 133 104 98 601.0 0 98 105 103 601.0 0 103 106 104 601.0 0 104 107 103 601.0 0 103 108 104 601.0 0 104 109 103 601.0 0 103 110 100 601.0 0 100 111 135 601.0 0 135 112 101 601.0 0 101 113 99 601.0 0 99 114 106 601.0 0 106 115 114 601.0 0 114 116 125 601.0 0 125 117 79 601.0 0 79 118 903 601.0 0 903 119 132 601.0 0 132 120 265 601.0 0 265 121 119 601.0 0 119 122 115 601.0 0 115 123 218 601.0 0 218 124 183 601.0 0 183 125 155 601.0 0 155 126 93 601.0 0 93 127 77 601.0 0 77 128 69 601.0 0 69 129 110 601.0 0 110 130 96 601.0 0 96 131 89 601.0 0 89 132 96 601.0 0 96 133 88 601.0 0 88 134 84 601.0 0 84 135 97 601.0 0 97 136 91 601.0 0 91 137 125 601.0 0 125 138 131 601.0 0 131 139 166 601.0 0 166 140 180 601.0 0 180 141 168 601.0 0 168 142 187 601.0 0 187 143 145 601.0 0 145 144 84 601.0 0 84 145 91 601.0 0 91 146 99 601.0 0 99 147 117 601.0 0 117 148 109 601.0 0 109 149 73 601.0 0 73 150 76 601.0 0 76 151 71 601.0 0 71 152 64 601.0 0 64 153 102 601.0 0 102 154 118 601.0 0 118 155 115 601.0 0 115 156 108 601.0 0 108 157 155 601.0 0 155 158 430 601.0 0 430 159 3846 601.0 0 3846 160 887 601.0 0 887 161 1443 601.0 0 1443 162 79 601.0 0 79 163 70 601.0 0 70 164 75 601.0 0 75 165 84 601.0 0 84 166 77 601.0 0 77 167 79 601.0 0 79 168 83 601.0 0 83 169 85 601.0 0 85 170 79 601.0 0 79 171 68 601.0 0 68 172 115 601.0 0 115 173 117 601.0 0 117 174 139 601.0 0 139 175 145 601.0 0 145 176 81 601.0 0 81 177 58 601.0 0 58 178 49 601.0 0 49 179 79 601.0 0 79 180 106 601.0 0 106 181 100 601.0 0 100 182 102 601.0 0 102 183 71 601.0 0 71 184 79 601.0 0 79 185 94 601.0 0 94 186 80 601.0 0 80 187 91 601.0 0 91 188 90 601.0 0 90 189 70 601.0 0 70 190 84 601.0 0 84 191 60 601.0 0 60 192 94 601.0 0 94 193 93 601.0 0 93 194 124 601.0 0 124 195 88 601.0 0 88 196 67 601.0 0 67 197 91 601.0 0 91 198 107 601.0 0 107 199 100 601.0 0 100 200 92 601.0 0 92 201 73 601.0 0 73 202 83 601.0 0 83 203 105 601.0 0 105 204 87 601.0 0 87 205 126 601.0 0 126 206 298 601.0 0 298 207 356 601.0 0 356 208 208 601.0 0 208 209 180 601.0 0 180 210 175 601.0 0 175 211 176 601.0 0 176 212 594 601.0 0 594 213 307 601.0 0 307 214 217 601.0 0 217 215 77 601.0 0 77 216 98 601.0 0 98 217 108 601.0 0 108 218 308 601.0 0 308 219 170 601.0 0 170 220 140 601.0 0 140 221 133 601.0 0 133 222 106 601.0 0 106 223 140 601.0 0 140 224 131 601.0 0 131 225 227 601.0 0 227 226 116 601.0 0 116 227 175 601.0 0 175 228 422 601.0 0 422 229 209 601.0 0 209 230 144 601.0 0 144 231 156 601.0 0 156 232 956 601.0 0 956 233 700 601.0 0 700 234 663 601.0 0 663 235 152 601.0 0 152 236 117 601.0 0 117 237 257 601.0 0 257 238 492 601.0 0 492 239 172 601.0 0 172 240 256 601.0 0 256 241 210 601.0 0 210 242 171 601.0 0 171 243 284 601.0 0 284 244 189 601.0 0 189 245 145 601.0 0 145 246 114 601.0 0 114 247 174 601.0 0 174 248 312 601.0 0 312 249 384 601.0 0 384 250 662 601.0 0 662 251 902 601.0 0 902 252 1182 601.0 0 1182 253 1843 601.0 0 1843 254 6598 601.0 0 6598 255 40851 601.0 0 40851 256 59678 601.0 0 59678 257 70041 601.0 0 70041 258 1340 601.0 0 1340 259 127 601.0 0 127 260 105 601.0 0 105 261 139 601.0 0 139 262 272 601.0 0 272 263 240 601.0 0 240 264 116 601.0 0 116 265 105 601.0 0 105 266 234 601.0 0 234 267 136 601.0 0 136 268 87 601.0 0 87 269 56 601.0 0 56 270 49 601.0 0 49 271 46 601.0 0 46 272 60 601.0 0 60 273 53 601.0 0 53 274 83 601.0 0 83 275 56 601.0 0 56 276 50 601.0 0 50 277 65 601.0 0 65 278 65 601.0 0 65 279 71 601.0 0 71 280 67 601.0 0 67 281 72 601.0 0 72 282 69 601.0 0 69 283 74 601.0 0 74 284 103 601.0 0 103 285 60 601.0 0 60 286 48 601.0 0 48 287 64 601.0 0 64 288 65 601.0 0 65 289 43 601.0 0 43 290 54 601.0 0 54 291 61 601.0 0 61 292 68 601.0 0 68 293 67 601.0 0 67 294 87 601.0 0 87 295 59 601.0 0 59 296 63 601.0 0 63 297 55 601.0 0 55 298 64 601.0 0 64 299 65 601.0 0 65 300 126 601.0 0 126 301 227 601.0 0 227 Finished in 1.80 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 39,875 Reads with adapters: 6,261 (15.7%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 6,245 (15.7%) Total basepairs processed: 8,801,905 bp Total written (filtered): 1,709,748 bp (19.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6261 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 2 19 68 0.0 3 0 51 8 5 4 20 6117 0.0 4 5669 338 79 24 7 21 26 0.0 4 12 10 4 22 2 0.0 4 0 0 0 0 2 23 19 0.0 4 2 0 0 11 6 26 1 0.0 4 1 28 1 0.0 4 0 0 0 0 1 29 1 0.0 4 1 37 1 0.0 4 1 61 2 0.0 4 0 0 0 0 2 69 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 1 116 2 0.0 4 0 0 2 124 1 0.0 4 0 0 0 0 1 127 4 0.0 4 0 0 0 0 4 130 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 1 222 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.21 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 6,245 Reads with adapters: 263 (4.2%) Reads written (passing filters): 6,245 (100.0%) Total basepairs processed: 1,709,748 bp Total written (filtered): 1,699,006 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 263 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 26.6% C: 19.0% G: 52.9% T: 1.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 40 97.6 0 40 4 7 24.4 0 7 9 3 0.0 0 1 2 10 5 0.0 1 0 5 11 97 0.0 1 3 94 12 60 0.0 1 1 59 13 2 0.0 1 0 2 20 4 0.0 1 4 21 2 0.0 1 1 1 24 1 0.0 1 1 48 1 0.0 1 1 152 1 0.0 1 1 179 1 0.0 1 1 199 1 0.0 1 1 210 38 0.0 1 34 4