This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 63.06 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 98,722 (4.0%) Reads that were too short: 45,893 (1.9%) Reads written (passing filters): 52,829 (2.2%) Total basepairs processed: 735,190,694 bp Total written (filtered): 7,965,263 bp (1.1%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 98722 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 402 596.3 0 402 7 200 596.3 0 200 8 2744 596.3 0 2744 9 2184 596.3 0 2184 10 28 596.3 0 28 11 22 596.3 0 22 12 20 596.3 0 20 13 21 596.3 0 21 14 24 596.3 0 24 15 19 596.3 0 19 16 29 596.3 0 29 17 34 596.3 0 34 18 35 596.3 0 35 19 26 596.3 0 26 20 26 596.3 0 26 21 42 596.3 0 42 22 39 596.3 0 39 23 46 596.3 0 46 24 30 596.3 0 30 25 24 596.3 0 24 26 72 596.3 0 72 27 69 596.3 0 69 28 28 596.3 0 28 29 51 596.3 0 51 30 26 596.3 0 26 31 24 596.3 0 24 32 31 596.3 0 31 33 42 596.3 0 42 34 32 596.3 0 32 35 26 596.3 0 26 36 23 596.3 0 23 37 33 596.3 0 33 38 24 596.3 0 24 39 26 596.3 0 26 40 22 596.3 0 22 41 31 596.3 0 31 42 23 596.3 0 23 43 30 596.3 0 30 44 25 596.3 0 25 45 25 596.3 0 25 46 24 596.3 0 24 47 25 596.3 0 25 48 20 596.3 0 20 49 20 596.3 0 20 50 22 596.3 0 22 51 43 596.3 0 43 52 34 596.3 0 34 53 21 596.3 0 21 54 42 596.3 0 42 55 22 596.3 0 22 56 15 596.3 0 15 57 37 596.3 0 37 58 18 596.3 0 18 59 31 596.3 0 31 60 14 596.3 0 14 61 32 596.3 0 32 62 18 596.3 0 18 63 17 596.3 0 17 64 47 596.3 0 47 65 40 596.3 0 40 66 66 596.3 0 66 67 31 596.3 0 31 68 33 596.3 0 33 69 55 596.3 0 55 70 67 596.3 0 67 71 86 596.3 0 86 72 20 596.3 0 20 73 22 596.3 0 22 74 40 596.3 0 40 75 36 596.3 0 36 76 51 596.3 0 51 77 60 596.3 0 60 78 27 596.3 0 27 79 81 596.3 0 81 80 146 596.3 0 146 81 226 596.3 0 226 82 56 596.3 0 56 83 35 596.3 0 35 84 50 596.3 0 50 85 39 596.3 0 39 86 34 596.3 0 34 87 29 596.3 0 29 88 29 596.3 0 29 89 48 596.3 0 48 90 29 596.3 0 29 91 31 596.3 0 31 92 28 596.3 0 28 93 23 596.3 0 23 94 34 596.3 0 34 95 43 596.3 0 43 96 35 596.3 0 35 97 34 596.3 0 34 98 35 596.3 0 35 99 59 596.3 0 59 100 72 596.3 0 72 101 98 596.3 0 98 102 39 596.3 0 39 103 25 596.3 0 25 104 35 596.3 0 35 105 32 596.3 0 32 106 31 596.3 0 31 107 47 596.3 0 47 108 31 596.3 0 31 109 28 596.3 0 28 110 46 596.3 0 46 111 62 596.3 0 62 112 104 596.3 0 104 113 143 596.3 0 143 114 94 596.3 0 94 115 49 596.3 0 49 116 44 596.3 0 44 117 308 596.3 0 308 118 278 596.3 0 278 119 181 596.3 0 181 120 55 596.3 0 55 121 48 596.3 0 48 122 60 596.3 0 60 123 52 596.3 0 52 124 39 596.3 0 39 125 45 596.3 0 45 126 47 596.3 0 47 127 73 596.3 0 73 128 181 596.3 0 181 129 153 596.3 0 153 130 320 596.3 0 320 131 329 596.3 0 329 132 363 596.3 0 363 133 294 596.3 0 294 134 85 596.3 0 85 135 115 596.3 0 115 136 160 596.3 0 160 137 287 596.3 0 287 138 236 596.3 0 236 139 89 596.3 0 89 140 75 596.3 0 75 141 51 596.3 0 51 142 37 596.3 0 37 143 50 596.3 0 50 144 41 596.3 0 41 145 64 596.3 0 64 146 111 596.3 0 111 147 106 596.3 0 106 148 42 596.3 0 42 149 44 596.3 0 44 150 46 596.3 0 46 151 41 596.3 0 41 152 44 596.3 0 44 153 43 596.3 0 43 154 80 596.3 0 80 155 47 596.3 0 47 156 76 596.3 0 76 157 133 596.3 0 133 158 195 596.3 0 195 159 54 596.3 0 54 160 69 596.3 0 69 161 79 596.3 0 79 162 93 596.3 0 93 163 95 596.3 0 95 164 86 596.3 0 86 165 103 596.3 0 103 166 116 596.3 0 116 167 114 596.3 0 114 168 173 596.3 0 173 169 157 596.3 0 157 170 124 596.3 0 124 171 162 596.3 0 162 172 116 596.3 0 116 173 146 596.3 0 146 174 163 596.3 0 163 175 221 596.3 0 221 176 818 596.3 0 818 177 3864 596.3 0 3864 178 7367 596.3 0 7367 179 1603 596.3 0 1603 180 2728 596.3 0 2728 181 3281 596.3 0 3281 182 4475 596.3 0 4475 183 3420 596.3 0 3420 184 867 596.3 0 867 185 210 596.3 0 210 186 103 596.3 0 103 187 83 596.3 0 83 188 92 596.3 0 92 189 122 596.3 0 122 190 85 596.3 0 85 191 91 596.3 0 91 192 100 596.3 0 100 193 95 596.3 0 95 194 136 596.3 0 136 195 387 596.3 0 387 196 1478 596.3 0 1478 197 1884 596.3 0 1884 198 1296 596.3 0 1296 199 451 596.3 0 451 200 134 596.3 0 134 201 566 596.3 0 566 202 1353 596.3 0 1353 203 1635 596.3 0 1635 204 686 596.3 0 686 205 48 596.3 0 48 206 40 596.3 0 40 207 36 596.3 0 36 208 51 596.3 0 51 209 63 596.3 0 63 210 47 596.3 0 47 211 38 596.3 0 38 212 32 596.3 0 32 213 51 596.3 0 51 214 51 596.3 0 51 215 121 596.3 0 121 216 172 596.3 0 172 217 179 596.3 0 179 218 115 596.3 0 115 219 75 596.3 0 75 220 47 596.3 0 47 221 91 596.3 0 91 222 345 596.3 0 345 223 734 596.3 0 734 224 726 596.3 0 726 225 370 596.3 0 370 226 75 596.3 0 75 227 54 596.3 0 54 228 52 596.3 0 52 229 50 596.3 0 50 230 47 596.3 0 47 231 70 596.3 0 70 232 53 596.3 0 53 233 100 596.3 0 100 234 196 596.3 0 196 235 302 596.3 0 302 236 459 596.3 0 459 237 161 596.3 0 161 238 121 596.3 0 121 239 545 596.3 0 545 240 1116 596.3 0 1116 241 1225 596.3 0 1225 242 419 596.3 0 419 243 448 596.3 0 448 244 348 596.3 0 348 245 304 596.3 0 304 246 142 596.3 0 142 247 94 596.3 0 94 248 79 596.3 0 79 249 104 596.3 0 104 250 146 596.3 0 146 251 440 596.3 0 440 252 319 596.3 0 319 253 225 596.3 0 225 254 206 596.3 0 206 255 204 596.3 0 204 256 239 596.3 0 239 257 556 596.3 0 556 258 678 596.3 0 678 259 1682 596.3 0 1682 260 5774 596.3 0 5774 261 2457 596.3 0 2457 262 278 596.3 0 278 263 297 596.3 0 297 264 1231 596.3 0 1231 265 3813 596.3 0 3813 266 3080 596.3 0 3080 267 1816 596.3 0 1816 268 36 596.3 0 36 269 69 596.3 0 69 270 105 596.3 0 105 271 74 596.3 0 74 272 88 596.3 0 88 273 92 596.3 0 92 274 142 596.3 0 142 275 263 596.3 0 263 276 207 596.3 0 207 277 266 596.3 0 266 278 325 596.3 0 325 279 223 596.3 0 223 280 51 596.3 0 51 281 35 596.3 0 35 282 41 596.3 0 41 283 55 596.3 0 55 284 48 596.3 0 48 285 76 596.3 0 76 286 67 596.3 0 67 287 83 596.3 0 83 288 75 596.3 0 75 289 72 596.3 0 72 290 135 596.3 0 135 291 1012 596.3 0 1012 292 1843 596.3 0 1843 293 1645 596.3 0 1645 294 425 596.3 0 425 295 102 596.3 0 102 296 129 596.3 0 129 297 233 596.3 0 233 298 158 596.3 0 158 299 175 596.3 0 175 300 243 596.3 0 243 301 189 596.3 0 189 Finished in 1.89 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 52,829 Reads with adapters: 2,463 (4.7%) Reads that were too short: 31 (0.1%) Reads written (passing filters): 2,432 (4.6%) Total basepairs processed: 7,965,263 bp Total written (filtered): 660,174 bp (8.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2463 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 2 2 18 2 0.0 3 0 0 1 1 19 32 0.0 3 0 26 1 2 3 20 2231 0.0 4 2069 127 21 12 2 21 123 0.0 4 74 27 11 8 3 22 16 0.0 4 12 2 0 1 1 23 9 0.0 4 0 0 0 7 2 24 1 0.0 4 0 0 0 1 25 1 0.0 4 1 29 4 0.0 4 0 0 0 0 4 32 1 0.0 4 0 0 0 0 1 34 2 0.0 4 0 0 0 0 2 39 5 0.0 4 0 0 0 0 5 40 6 0.0 4 0 0 0 1 5 41 2 0.0 4 0 0 0 0 2 43 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 1 99 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 113 3 0.0 4 0 0 0 0 3 122 2 0.0 4 0 0 0 0 2 126 1 0.0 4 0 0 0 0 1 127 3 0.0 4 0 0 0 0 3 149 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.09 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 2,432 Reads with adapters: 546 (22.5%) Reads written (passing filters): 2,432 (100.0%) Total basepairs processed: 660,174 bp Total written (filtered): 653,052 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 546 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.3% C: 10.4% G: 84.1% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 38.0 0 1 4 1 9.5 0 1 9 147 0.0 0 14 133 10 327 0.0 1 24 303 11 58 0.0 1 1 57 12 4 0.0 1 0 4 13 1 0.0 1 0 1 208 1 0.0 1 0 1 268 1 0.0 1 1 269 3 0.0 1 2 1 270 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.