This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.50 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 75,601 (3.1%) Reads that were too short: 33,077 (1.4%) Reads written (passing filters): 42,524 (1.7%) Total basepairs processed: 735,190,694 bp Total written (filtered): 6,458,844 bp (0.9%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 75601 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 364 596.3 0 364 7 113 596.3 0 113 8 2398 596.3 0 2398 9 2553 596.3 0 2553 10 14 596.3 0 14 11 19 596.3 0 19 12 18 596.3 0 18 13 20 596.3 0 20 14 5 596.3 0 5 15 14 596.3 0 14 16 20 596.3 0 20 17 18 596.3 0 18 18 15 596.3 0 15 19 14 596.3 0 14 20 16 596.3 0 16 21 31 596.3 0 31 22 22 596.3 0 22 23 22 596.3 0 22 24 17 596.3 0 17 25 7 596.3 0 7 26 21 596.3 0 21 27 23 596.3 0 23 28 29 596.3 0 29 29 44 596.3 0 44 30 26 596.3 0 26 31 15 596.3 0 15 32 10 596.3 0 10 33 28 596.3 0 28 34 23 596.3 0 23 35 18 596.3 0 18 36 26 596.3 0 26 37 36 596.3 0 36 38 25 596.3 0 25 39 13 596.3 0 13 40 25 596.3 0 25 41 31 596.3 0 31 42 13 596.3 0 13 43 9 596.3 0 9 44 26 596.3 0 26 45 19 596.3 0 19 46 16 596.3 0 16 47 27 596.3 0 27 48 16 596.3 0 16 49 18 596.3 0 18 50 27 596.3 0 27 51 19 596.3 0 19 52 17 596.3 0 17 53 22 596.3 0 22 54 21 596.3 0 21 55 23 596.3 0 23 56 18 596.3 0 18 57 18 596.3 0 18 58 14 596.3 0 14 59 19 596.3 0 19 60 16 596.3 0 16 61 21 596.3 0 21 62 17 596.3 0 17 63 18 596.3 0 18 64 23 596.3 0 23 65 32 596.3 0 32 66 38 596.3 0 38 67 24 596.3 0 24 68 21 596.3 0 21 69 31 596.3 0 31 70 82 596.3 0 82 71 81 596.3 0 81 72 15 596.3 0 15 73 20 596.3 0 20 74 24 596.3 0 24 75 20 596.3 0 20 76 21 596.3 0 21 77 20 596.3 0 20 78 27 596.3 0 27 79 55 596.3 0 55 80 105 596.3 0 105 81 159 596.3 0 159 82 21 596.3 0 21 83 13 596.3 0 13 84 11 596.3 0 11 85 10 596.3 0 10 86 14 596.3 0 14 87 15 596.3 0 15 88 15 596.3 0 15 89 17 596.3 0 17 90 16 596.3 0 16 91 17 596.3 0 17 92 13 596.3 0 13 93 20 596.3 0 20 94 14 596.3 0 14 95 16 596.3 0 16 96 15 596.3 0 15 97 20 596.3 0 20 98 23 596.3 0 23 99 37 596.3 0 37 100 31 596.3 0 31 101 41 596.3 0 41 102 19 596.3 0 19 103 28 596.3 0 28 104 18 596.3 0 18 105 14 596.3 0 14 106 21 596.3 0 21 107 13 596.3 0 13 108 18 596.3 0 18 109 14 596.3 0 14 110 18 596.3 0 18 111 30 596.3 0 30 112 38 596.3 0 38 113 92 596.3 0 92 114 56 596.3 0 56 115 19 596.3 0 19 116 26 596.3 0 26 117 102 596.3 0 102 118 105 596.3 0 105 119 55 596.3 0 55 120 22 596.3 0 22 121 28 596.3 0 28 122 18 596.3 0 18 123 21 596.3 0 21 124 22 596.3 0 22 125 28 596.3 0 28 126 31 596.3 0 31 127 41 596.3 0 41 128 92 596.3 0 92 129 131 596.3 0 131 130 221 596.3 0 221 131 81 596.3 0 81 132 109 596.3 0 109 133 88 596.3 0 88 134 58 596.3 0 58 135 87 596.3 0 87 136 124 596.3 0 124 137 275 596.3 0 275 138 170 596.3 0 170 139 51 596.3 0 51 140 47 596.3 0 47 141 21 596.3 0 21 142 16 596.3 0 16 143 33 596.3 0 33 144 36 596.3 0 36 145 37 596.3 0 37 146 66 596.3 0 66 147 45 596.3 0 45 148 38 596.3 0 38 149 26 596.3 0 26 150 24 596.3 0 24 151 30 596.3 0 30 152 28 596.3 0 28 153 28 596.3 0 28 154 34 596.3 0 34 155 35 596.3 0 35 156 43 596.3 0 43 157 91 596.3 0 91 158 117 596.3 0 117 159 26 596.3 0 26 160 49 596.3 0 49 161 38 596.3 0 38 162 71 596.3 0 71 163 107 596.3 0 107 164 59 596.3 0 59 165 102 596.3 0 102 166 109 596.3 0 109 167 115 596.3 0 115 168 153 596.3 0 153 169 114 596.3 0 114 170 102 596.3 0 102 171 168 596.3 0 168 172 87 596.3 0 87 173 132 596.3 0 132 174 122 596.3 0 122 175 143 596.3 0 143 176 635 596.3 0 635 177 3510 596.3 0 3510 178 6761 596.3 0 6761 179 1438 596.3 0 1438 180 2857 596.3 0 2857 181 3192 596.3 0 3192 182 4128 596.3 0 4128 183 3149 596.3 0 3149 184 316 596.3 0 316 185 90 596.3 0 90 186 47 596.3 0 47 187 43 596.3 0 43 188 42 596.3 0 42 189 54 596.3 0 54 190 25 596.3 0 25 191 50 596.3 0 50 192 57 596.3 0 57 193 52 596.3 0 52 194 68 596.3 0 68 195 183 596.3 0 183 196 666 596.3 0 666 197 971 596.3 0 971 198 958 596.3 0 958 199 304 596.3 0 304 200 85 596.3 0 85 201 283 596.3 0 283 202 738 596.3 0 738 203 1151 596.3 0 1151 204 294 596.3 0 294 205 18 596.3 0 18 206 21 596.3 0 21 207 32 596.3 0 32 208 21 596.3 0 21 209 32 596.3 0 32 210 29 596.3 0 29 211 21 596.3 0 21 212 27 596.3 0 27 213 35 596.3 0 35 214 32 596.3 0 32 215 76 596.3 0 76 216 115 596.3 0 115 217 90 596.3 0 90 218 74 596.3 0 74 219 31 596.3 0 31 220 24 596.3 0 24 221 48 596.3 0 48 222 166 596.3 0 166 223 386 596.3 0 386 224 398 596.3 0 398 225 263 596.3 0 263 226 58 596.3 0 58 227 29 596.3 0 29 228 25 596.3 0 25 229 41 596.3 0 41 230 38 596.3 0 38 231 37 596.3 0 37 232 44 596.3 0 44 233 44 596.3 0 44 234 166 596.3 0 166 235 335 596.3 0 335 236 461 596.3 0 461 237 174 596.3 0 174 238 56 596.3 0 56 239 420 596.3 0 420 240 708 596.3 0 708 241 935 596.3 0 935 242 199 596.3 0 199 243 183 596.3 0 183 244 111 596.3 0 111 245 224 596.3 0 224 246 105 596.3 0 105 247 69 596.3 0 69 248 61 596.3 0 61 249 90 596.3 0 90 250 116 596.3 0 116 251 383 596.3 0 383 252 212 596.3 0 212 253 108 596.3 0 108 254 120 596.3 0 120 255 162 596.3 0 162 256 138 596.3 0 138 257 366 596.3 0 366 258 317 596.3 0 317 259 1121 596.3 0 1121 260 5931 596.3 0 5931 261 1764 596.3 0 1764 262 176 596.3 0 176 263 163 596.3 0 163 264 715 596.3 0 715 265 3110 596.3 0 3110 266 2034 596.3 0 2034 267 1502 596.3 0 1502 268 24 596.3 0 24 269 30 596.3 0 30 270 50 596.3 0 50 271 44 596.3 0 44 272 33 596.3 0 33 273 25 596.3 0 25 274 47 596.3 0 47 275 73 596.3 0 73 276 78 596.3 0 78 277 70 596.3 0 70 278 92 596.3 0 92 279 47 596.3 0 47 280 24 596.3 0 24 281 40 596.3 0 40 282 28 596.3 0 28 283 25 596.3 0 25 284 33 596.3 0 33 285 38 596.3 0 38 286 52 596.3 0 52 287 68 596.3 0 68 288 59 596.3 0 59 289 51 596.3 0 51 290 84 596.3 0 84 291 738 596.3 0 738 292 1393 596.3 0 1393 293 1312 596.3 0 1312 294 368 596.3 0 368 295 92 596.3 0 92 296 95 596.3 0 95 297 229 596.3 0 229 298 109 596.3 0 109 299 111 596.3 0 111 300 236 596.3 0 236 301 106 596.3 0 106 Finished in 1.38 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 42,524 Reads with adapters: 2,870 (6.7%) Reads that were too short: 176 (0.4%) Reads written (passing filters): 2,694 (6.3%) Total basepairs processed: 6,458,844 bp Total written (filtered): 737,348 bp (11.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2870 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 26 0.0 3 0 22 4 17 2296 0.0 3 2230 63 3 18 28 0.0 3 20 6 1 1 19 11 0.0 3 6 2 3 20 287 0.0 3 274 5 0 8 21 4 0.0 3 0 4 22 1 0.0 3 0 0 0 1 27 3 0.0 3 0 0 0 3 32 1 0.0 3 0 0 0 1 35 10 0.0 3 0 0 0 10 36 1 0.0 3 0 0 0 1 38 4 0.0 3 1 0 0 3 40 1 0.0 3 1 49 1 0.0 3 0 0 0 1 50 95 0.0 3 0 0 0 95 54 1 0.0 3 0 0 0 1 60 5 0.0 3 0 0 0 5 62 2 0.0 3 0 0 0 2 63 2 0.0 3 0 0 0 2 74 3 0.0 3 0 0 0 3 80 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 85 1 0.0 3 1 90 5 0.0 3 0 0 0 5 91 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 4 0.0 3 0 0 0 4 100 40 0.0 3 0 0 1 39 101 1 0.0 3 0 0 0 1 104 3 0.0 3 0 0 0 3 105 4 0.0 3 0 0 1 3 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 120 4 0.0 3 0 0 0 4 130 7 0.0 3 0 0 0 7 140 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.10 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 2,694 Reads with adapters: 21 (0.8%) Reads written (passing filters): 2,694 (100.0%) Total basepairs processed: 737,348 bp Total written (filtered): 737,283 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 21 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 66.7% C: 4.8% G: 19.0% T: 9.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 20 42.1 0 20 5 1 2.6 0 1