This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.01 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 230,063 (9.4%) Reads that were too short: 162,719 (6.7%) Reads written (passing filters): 67,344 (2.8%) Total basepairs processed: 735,190,694 bp Total written (filtered): 15,278,622 bp (2.1%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 230063 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 350 596.3 0 350 7 2966 596.3 0 2966 8 3078 596.3 0 3078 9 45 596.3 0 45 10 27 596.3 0 27 11 22 596.3 0 22 12 12 596.3 0 12 13 25 596.3 0 25 14 29 596.3 0 29 15 24 596.3 0 24 16 14 596.3 0 14 17 19 596.3 0 19 18 21 596.3 0 21 19 15 596.3 0 15 20 15 596.3 0 15 21 31 596.3 0 31 22 11 596.3 0 11 23 14 596.3 0 14 24 19 596.3 0 19 25 25 596.3 0 25 26 14 596.3 0 14 27 13 596.3 0 13 28 12 596.3 0 12 29 23 596.3 0 23 30 18 596.3 0 18 31 20 596.3 0 20 32 19 596.3 0 19 33 25 596.3 0 25 34 11 596.3 0 11 35 22 596.3 0 22 36 10 596.3 0 10 37 23 596.3 0 23 38 15 596.3 0 15 39 17 596.3 0 17 40 32 596.3 0 32 41 12 596.3 0 12 42 27 596.3 0 27 43 21 596.3 0 21 44 29 596.3 0 29 45 14 596.3 0 14 46 17 596.3 0 17 47 16 596.3 0 16 48 29 596.3 0 29 49 34 596.3 0 34 50 42 596.3 0 42 51 99 596.3 0 99 52 57 596.3 0 57 53 30 596.3 0 30 54 38 596.3 0 38 55 47 596.3 0 47 56 76 596.3 0 76 57 43 596.3 0 43 58 54 596.3 0 54 59 56 596.3 0 56 60 64 596.3 0 64 61 163 596.3 0 163 62 334 596.3 0 334 63 91 596.3 0 91 64 137 596.3 0 137 65 264 596.3 0 264 66 117 596.3 0 117 67 77 596.3 0 77 68 226 596.3 0 226 69 196 596.3 0 196 70 247 596.3 0 247 71 297 596.3 0 297 72 363 596.3 0 363 73 475 596.3 0 475 74 613 596.3 0 613 75 16480 596.3 0 16480 76 24791 596.3 0 24791 77 9599 596.3 0 9599 78 45 596.3 0 45 79 24 596.3 0 24 80 21 596.3 0 21 81 28 596.3 0 28 82 14 596.3 0 14 83 33 596.3 0 33 84 15 596.3 0 15 85 9 596.3 0 9 86 14 596.3 0 14 87 13 596.3 0 13 88 16 596.3 0 16 89 19 596.3 0 19 90 14 596.3 0 14 91 32 596.3 0 32 92 10 596.3 0 10 93 11 596.3 0 11 94 13 596.3 0 13 95 28 596.3 0 28 96 18 596.3 0 18 97 10 596.3 0 10 98 21 596.3 0 21 99 17 596.3 0 17 100 12 596.3 0 12 101 12 596.3 0 12 102 25 596.3 0 25 103 14 596.3 0 14 104 19 596.3 0 19 105 31 596.3 0 31 106 18 596.3 0 18 107 16 596.3 0 16 108 21 596.3 0 21 109 15 596.3 0 15 110 13 596.3 0 13 111 10 596.3 0 10 112 17 596.3 0 17 113 9 596.3 0 9 114 12 596.3 0 12 115 17 596.3 0 17 116 18 596.3 0 18 117 17 596.3 0 17 118 22 596.3 0 22 119 29 596.3 0 29 120 19 596.3 0 19 121 27 596.3 0 27 122 11 596.3 0 11 123 23 596.3 0 23 124 16 596.3 0 16 125 144 596.3 0 144 126 85 596.3 0 85 127 82 596.3 0 82 128 46 596.3 0 46 129 35 596.3 0 35 130 24 596.3 0 24 131 16 596.3 0 16 132 195 596.3 0 195 133 174 596.3 0 174 134 22 596.3 0 22 135 134 596.3 0 134 136 135 596.3 0 135 137 89 596.3 0 89 138 22 596.3 0 22 139 13 596.3 0 13 140 21 596.3 0 21 141 24 596.3 0 24 142 19 596.3 0 19 143 16 596.3 0 16 144 94 596.3 0 94 145 66 596.3 0 66 146 21 596.3 0 21 147 14 596.3 0 14 148 11 596.3 0 11 149 20 596.3 0 20 150 26 596.3 0 26 151 16 596.3 0 16 152 16 596.3 0 16 153 10 596.3 0 10 154 27 596.3 0 27 155 34 596.3 0 34 156 19 596.3 0 19 157 53 596.3 0 53 158 16 596.3 0 16 159 13 596.3 0 13 160 16 596.3 0 16 161 22 596.3 0 22 162 38 596.3 0 38 163 41 596.3 0 41 164 32 596.3 0 32 165 32 596.3 0 32 166 41 596.3 0 41 167 59 596.3 0 59 168 66 596.3 0 66 169 45 596.3 0 45 170 15 596.3 0 15 171 21 596.3 0 21 172 17 596.3 0 17 173 19 596.3 0 19 174 12 596.3 0 12 175 16 596.3 0 16 176 22 596.3 0 22 177 25 596.3 0 25 178 22 596.3 0 22 179 28 596.3 0 28 180 43 596.3 0 43 181 45 596.3 0 45 182 48 596.3 0 48 183 29 596.3 0 29 184 38 596.3 0 38 185 24 596.3 0 24 186 45 596.3 0 45 187 72 596.3 0 72 188 96 596.3 0 96 189 58 596.3 0 58 190 37 596.3 0 37 191 39 596.3 0 39 192 51 596.3 0 51 193 97 596.3 0 97 194 113 596.3 0 113 195 78 596.3 0 78 196 74 596.3 0 74 197 164 596.3 0 164 198 288 596.3 0 288 199 184 596.3 0 184 200 89 596.3 0 89 201 112 596.3 0 112 202 71 596.3 0 71 203 64 596.3 0 64 204 77 596.3 0 77 205 113 596.3 0 113 206 53 596.3 0 53 207 48 596.3 0 48 208 64 596.3 0 64 209 45 596.3 0 45 210 65 596.3 0 65 211 180 596.3 0 180 212 60 596.3 0 60 213 63 596.3 0 63 214 93 596.3 0 93 215 54 596.3 0 54 216 78 596.3 0 78 217 107 596.3 0 107 218 225 596.3 0 225 219 148 596.3 0 148 220 165 596.3 0 165 221 270 596.3 0 270 222 109 596.3 0 109 223 238 596.3 0 238 224 433 596.3 0 433 225 573 596.3 0 573 226 375 596.3 0 375 227 87 596.3 0 87 228 137 596.3 0 137 229 115 596.3 0 115 230 123 596.3 0 123 231 159 596.3 0 159 232 219 596.3 0 219 233 324 596.3 0 324 234 598 596.3 0 598 235 809 596.3 0 809 236 1148 596.3 0 1148 237 1831 596.3 0 1831 238 33146 596.3 0 33146 239 8825 596.3 0 8825 240 28050 596.3 0 28050 241 420 596.3 0 420 242 310 596.3 0 310 243 276 596.3 0 276 244 340 596.3 0 340 245 336 596.3 0 336 246 400 596.3 0 400 247 690 596.3 0 690 248 7531 596.3 0 7531 249 19891 596.3 0 19891 250 22320 596.3 0 22320 251 19604 596.3 0 19604 252 76 596.3 0 76 253 34 596.3 0 34 254 41 596.3 0 41 255 31 596.3 0 31 256 32 596.3 0 32 257 61 596.3 0 61 258 41 596.3 0 41 259 27 596.3 0 27 260 98 596.3 0 98 261 54 596.3 0 54 262 49 596.3 0 49 263 23 596.3 0 23 264 37 596.3 0 37 265 28 596.3 0 28 266 40 596.3 0 40 267 32 596.3 0 32 268 39 596.3 0 39 269 88 596.3 0 88 270 41 596.3 0 41 271 58 596.3 0 58 272 82 596.3 0 82 273 45 596.3 0 45 274 39 596.3 0 39 275 44 596.3 0 44 276 22 596.3 0 22 277 61 596.3 0 61 278 67 596.3 0 67 279 217 596.3 0 217 280 267 596.3 0 267 281 231 596.3 0 231 282 1136 596.3 0 1136 283 1760 596.3 0 1760 284 2387 596.3 0 2387 285 387 596.3 0 387 286 142 596.3 0 142 287 114 596.3 0 114 288 83 596.3 0 83 289 50 596.3 0 50 290 34 596.3 0 34 291 54 596.3 0 54 292 65 596.3 0 65 293 55 596.3 0 55 294 307 596.3 0 307 295 402 596.3 0 402 296 848 596.3 0 848 297 271 596.3 0 271 298 155 596.3 0 155 299 226 596.3 0 226 300 471 596.3 0 471 301 307 596.3 0 307 Finished in 2.72 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 67,344 Reads with adapters: 3,649 (5.4%) Reads that were too short: 37 (0.1%) Reads written (passing filters): 3,612 (5.4%) Total basepairs processed: 15,278,622 bp Total written (filtered): 882,732 bp (5.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3649 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 34 0.0 3 0 32 2 17 2795 0.0 3 2738 55 2 18 9 0.0 3 0 8 0 1 19 1 0.0 3 0 0 1 20 3 0.0 3 0 0 0 3 22 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 1 60 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 65 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 91 17 0.0 3 0 0 0 17 92 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 96 54 0.0 3 0 0 0 54 97 3 0.0 3 0 0 0 3 98 343 0.0 3 0 0 4 339 99 116 0.0 3 0 0 0 116 100 5 0.0 3 0 0 0 5 101 38 0.0 3 0 0 4 34 102 9 0.0 3 0 0 0 9 104 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 109 7 0.0 3 0 0 0 7 110 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 7 0.0 3 0 0 0 7 116 75 0.0 3 0 0 0 75 117 32 0.0 3 0 0 0 32 118 3 0.0 3 0 0 0 3 119 13 0.0 3 0 0 0 13 120 3 0.0 3 0 0 0 3 123 2 0.0 3 0 0 0 2 124 9 0.0 3 0 0 0 9 125 4 0.0 3 0 0 0 4 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 176 4 0.0 3 0 0 0 4 182 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 1 199 1 0.0 3 0 0 1 204 2 0.0 3 0 0 0 2 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 218 3 0.0 3 0 0 0 3 222 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 3,612 Reads with adapters: 11 (0.3%) Reads written (passing filters): 3,612 (100.0%) Total basepairs processed: 882,732 bp Total written (filtered): 882,699 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 11 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 9.1% C: 36.4% G: 18.2% T: 36.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 11 56.4 0 11