This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.08 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 112,866 (4.6%) Reads that were too short: 38,316 (1.6%) Reads written (passing filters): 74,550 (3.1%) Total basepairs processed: 735,190,694 bp Total written (filtered): 14,063,061 bp (1.9%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 112866 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1094 596.3 0 1094 7 9905 596.3 0 9905 8 223 596.3 0 223 9 9708 596.3 0 9708 10 30 596.3 0 30 11 6 596.3 0 6 12 9 596.3 0 9 13 6 596.3 0 6 14 10 596.3 0 10 15 11 596.3 0 11 16 10 596.3 0 10 17 5 596.3 0 5 18 8 596.3 0 8 19 15 596.3 0 15 20 10 596.3 0 10 21 6 596.3 0 6 22 9 596.3 0 9 23 9 596.3 0 9 24 11 596.3 0 11 25 16 596.3 0 16 26 12 596.3 0 12 27 12 596.3 0 12 28 9 596.3 0 9 29 12 596.3 0 12 30 14 596.3 0 14 31 14 596.3 0 14 32 17 596.3 0 17 33 10 596.3 0 10 34 6 596.3 0 6 35 16 596.3 0 16 36 5 596.3 0 5 37 11 596.3 0 11 38 6 596.3 0 6 39 13 596.3 0 13 40 9 596.3 0 9 41 17 596.3 0 17 42 12 596.3 0 12 43 14 596.3 0 14 44 7 596.3 0 7 45 11 596.3 0 11 46 14 596.3 0 14 47 5 596.3 0 5 48 13 596.3 0 13 49 8 596.3 0 8 50 12 596.3 0 12 51 13 596.3 0 13 52 16 596.3 0 16 53 14 596.3 0 14 54 35 596.3 0 35 55 25 596.3 0 25 56 22 596.3 0 22 57 17 596.3 0 17 58 20 596.3 0 20 59 12 596.3 0 12 60 11 596.3 0 11 61 14 596.3 0 14 62 19 596.3 0 19 63 28 596.3 0 28 64 68 596.3 0 68 65 103 596.3 0 103 66 84 596.3 0 84 67 22 596.3 0 22 68 38 596.3 0 38 69 58 596.3 0 58 70 54 596.3 0 54 71 228 596.3 0 228 72 660 596.3 0 660 73 238 596.3 0 238 74 660 596.3 0 660 75 170 596.3 0 170 76 180 596.3 0 180 77 38 596.3 0 38 78 98 596.3 0 98 79 120 596.3 0 120 80 156 596.3 0 156 81 140 596.3 0 140 82 105 596.3 0 105 83 244 596.3 0 244 84 200 596.3 0 200 85 435 596.3 0 435 86 45 596.3 0 45 87 29 596.3 0 29 88 69 596.3 0 69 89 40 596.3 0 40 90 41 596.3 0 41 91 37 596.3 0 37 92 40 596.3 0 40 93 21 596.3 0 21 94 36 596.3 0 36 95 37 596.3 0 37 96 28 596.3 0 28 97 37 596.3 0 37 98 27 596.3 0 27 99 30 596.3 0 30 100 35 596.3 0 35 101 68 596.3 0 68 102 94 596.3 0 94 103 106 596.3 0 106 104 111 596.3 0 111 105 126 596.3 0 126 106 151 596.3 0 151 107 1219 596.3 0 1219 108 1034 596.3 0 1034 109 1592 596.3 0 1592 110 126 596.3 0 126 111 98 596.3 0 98 112 118 596.3 0 118 113 139 596.3 0 139 114 79 596.3 0 79 115 125 596.3 0 125 116 148 596.3 0 148 117 205 596.3 0 205 118 138 596.3 0 138 119 180 596.3 0 180 120 814 596.3 0 814 121 892 596.3 0 892 122 846 596.3 0 846 123 374 596.3 0 374 124 266 596.3 0 266 125 341 596.3 0 341 126 165 596.3 0 165 127 182 596.3 0 182 128 132 596.3 0 132 129 107 596.3 0 107 130 103 596.3 0 103 131 153 596.3 0 153 132 114 596.3 0 114 133 87 596.3 0 87 134 98 596.3 0 98 135 76 596.3 0 76 136 112 596.3 0 112 137 155 596.3 0 155 138 131 596.3 0 131 139 190 596.3 0 190 140 117 596.3 0 117 141 210 596.3 0 210 142 192 596.3 0 192 143 246 596.3 0 246 144 118 596.3 0 118 145 382 596.3 0 382 146 224 596.3 0 224 147 186 596.3 0 186 148 199 596.3 0 199 149 172 596.3 0 172 150 152 596.3 0 152 151 224 596.3 0 224 152 167 596.3 0 167 153 123 596.3 0 123 154 117 596.3 0 117 155 69 596.3 0 69 156 101 596.3 0 101 157 96 596.3 0 96 158 73 596.3 0 73 159 131 596.3 0 131 160 159 596.3 0 159 161 148 596.3 0 148 162 121 596.3 0 121 163 121 596.3 0 121 164 165 596.3 0 165 165 288 596.3 0 288 166 2309 596.3 0 2309 167 1839 596.3 0 1839 168 2096 596.3 0 2096 169 263 596.3 0 263 170 1084 596.3 0 1084 171 1380 596.3 0 1380 172 1146 596.3 0 1146 173 202 596.3 0 202 174 164 596.3 0 164 175 148 596.3 0 148 176 168 596.3 0 168 177 191 596.3 0 191 178 194 596.3 0 194 179 585 596.3 0 585 180 3000 596.3 0 3000 181 6223 596.3 0 6223 182 1391 596.3 0 1391 183 1157 596.3 0 1157 184 1882 596.3 0 1882 185 2915 596.3 0 2915 186 2222 596.3 0 2222 187 119 596.3 0 119 188 102 596.3 0 102 189 106 596.3 0 106 190 82 596.3 0 82 191 94 596.3 0 94 192 91 596.3 0 91 193 74 596.3 0 74 194 117 596.3 0 117 195 105 596.3 0 105 196 95 596.3 0 95 197 100 596.3 0 100 198 239 596.3 0 239 199 337 596.3 0 337 200 349 596.3 0 349 201 208 596.3 0 208 202 144 596.3 0 144 203 129 596.3 0 129 204 330 596.3 0 330 205 685 596.3 0 685 206 935 596.3 0 935 207 458 596.3 0 458 208 98 596.3 0 98 209 135 596.3 0 135 210 119 596.3 0 119 211 199 596.3 0 199 212 193 596.3 0 193 213 1137 596.3 0 1137 214 4366 596.3 0 4366 215 2467 596.3 0 2467 216 334 596.3 0 334 217 92 596.3 0 92 218 140 596.3 0 140 219 153 596.3 0 153 220 189 596.3 0 189 221 113 596.3 0 113 222 99 596.3 0 99 223 106 596.3 0 106 224 117 596.3 0 117 225 424 596.3 0 424 226 408 596.3 0 408 227 612 596.3 0 612 228 478 596.3 0 478 229 301 596.3 0 301 230 210 596.3 0 210 231 89 596.3 0 89 232 72 596.3 0 72 233 85 596.3 0 85 234 76 596.3 0 76 235 76 596.3 0 76 236 80 596.3 0 80 237 137 596.3 0 137 238 170 596.3 0 170 239 178 596.3 0 178 240 216 596.3 0 216 241 102 596.3 0 102 242 182 596.3 0 182 243 305 596.3 0 305 244 334 596.3 0 334 245 226 596.3 0 226 246 339 596.3 0 339 247 265 596.3 0 265 248 191 596.3 0 191 249 133 596.3 0 133 250 138 596.3 0 138 251 118 596.3 0 118 252 105 596.3 0 105 253 174 596.3 0 174 254 240 596.3 0 240 255 282 596.3 0 282 256 321 596.3 0 321 257 261 596.3 0 261 258 78 596.3 0 78 259 111 596.3 0 111 260 162 596.3 0 162 261 280 596.3 0 280 262 448 596.3 0 448 263 628 596.3 0 628 264 508 596.3 0 508 265 221 596.3 0 221 266 255 596.3 0 255 267 2337 596.3 0 2337 268 3690 596.3 0 3690 269 2892 596.3 0 2892 270 444 596.3 0 444 271 94 596.3 0 94 272 89 596.3 0 89 273 127 596.3 0 127 274 109 596.3 0 109 275 189 596.3 0 189 276 225 596.3 0 225 277 281 596.3 0 281 278 207 596.3 0 207 279 170 596.3 0 170 280 619 596.3 0 619 281 638 596.3 0 638 282 543 596.3 0 543 283 171 596.3 0 171 284 69 596.3 0 69 285 92 596.3 0 92 286 124 596.3 0 124 287 146 596.3 0 146 288 124 596.3 0 124 289 117 596.3 0 117 290 116 596.3 0 116 291 124 596.3 0 124 292 104 596.3 0 104 293 155 596.3 0 155 294 110 596.3 0 110 295 123 596.3 0 123 296 114 596.3 0 114 297 146 596.3 0 146 298 133 596.3 0 133 299 236 596.3 0 236 300 259 596.3 0 259 301 442 596.3 0 442 Finished in 3.00 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 74,550 Reads with adapters: 10,172 (13.6%) Reads that were too short: 57 (0.1%) Reads written (passing filters): 10,115 (13.6%) Total basepairs processed: 14,063,061 bp Total written (filtered): 2,763,465 bp (19.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10172 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 5 0.0 3 0 0 4 1 19 119 0.0 3 0 108 7 3 1 20 9853 0.0 4 9255 494 83 14 7 21 45 0.0 4 18 24 2 1 22 3 0.0 4 0 0 1 1 1 23 14 0.0 4 0 0 0 10 4 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 0 1 27 2 0.0 4 0 0 0 0 2 31 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 37 2 0.0 4 0 0 0 0 2 38 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 46 2 0.0 4 1 0 0 0 1 50 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 70 6 0.0 4 0 0 0 0 6 73 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 1 107 1 0.0 4 0 0 0 0 1 109 2 0.0 4 0 0 0 0 2 110 3 0.0 4 0 0 0 0 3 114 3 0.0 4 0 0 0 0 3 118 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 120 1 0.0 4 1 121 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 123 1 0.0 4 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 49 0.0 4 0 0 0 0 49 130 9 0.0 4 0 0 0 0 9 137 1 0.0 4 0 0 0 0 1 142 3 0.0 4 0 0 0 0 3 143 1 0.0 4 0 0 0 0 1 152 4 0.0 4 0 0 0 0 4 153 3 0.0 4 0 0 0 0 3 161 1 0.0 4 0 0 0 0 1 168 3 0.0 4 0 0 0 0 3 177 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.36 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 10,115 Reads with adapters: 1,644 (16.3%) Reads written (passing filters): 10,115 (100.0%) Total basepairs processed: 2,763,465 bp Total written (filtered): 2,537,422 bp (91.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1644 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.9% C: 62.2% G: 32.8% T: 1.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 46 158.0 0 46 4 4 39.5 0 4 9 3 0.0 0 0 3 10 18 0.0 1 2 16 11 461 0.0 1 20 441 12 126 0.0 1 1 125 13 4 0.0 1 0 4 14 1 0.0 1 0 1 15 1 0.0 1 0 1 20 5 0.0 1 4 1 152 4 0.0 1 3 1 153 1 0.0 1 0 1 210 9 0.0 1 3 6 222 1 0.0 1 0 1 224 2 0.0 1 2 225 911 0.0 1 826 85 226 47 0.0 1 43 4