This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 62.86 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 106,696 (4.4%) Reads that were too short: 35,251 (1.4%) Reads written (passing filters): 71,445 (2.9%) Total basepairs processed: 735,190,694 bp Total written (filtered): 13,666,778 bp (1.9%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 106696 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 791 596.3 0 791 7 11443 596.3 0 11443 8 136 596.3 0 136 9 9228 596.3 0 9228 10 18 596.3 0 18 11 17 596.3 0 17 12 14 596.3 0 14 13 14 596.3 0 14 14 8 596.3 0 8 15 12 596.3 0 12 16 8 596.3 0 8 17 7 596.3 0 7 18 17 596.3 0 17 19 11 596.3 0 11 20 20 596.3 0 20 21 10 596.3 0 10 22 9 596.3 0 9 23 21 596.3 0 21 24 10 596.3 0 10 25 12 596.3 0 12 26 12 596.3 0 12 27 13 596.3 0 13 28 15 596.3 0 15 29 14 596.3 0 14 30 23 596.3 0 23 31 12 596.3 0 12 32 12 596.3 0 12 33 11 596.3 0 11 34 15 596.3 0 15 35 18 596.3 0 18 36 15 596.3 0 15 37 17 596.3 0 17 38 10 596.3 0 10 39 17 596.3 0 17 40 11 596.3 0 11 41 43 596.3 0 43 42 15 596.3 0 15 43 21 596.3 0 21 44 8 596.3 0 8 45 11 596.3 0 11 46 18 596.3 0 18 47 16 596.3 0 16 48 26 596.3 0 26 49 18 596.3 0 18 50 18 596.3 0 18 51 18 596.3 0 18 52 18 596.3 0 18 53 15 596.3 0 15 54 17 596.3 0 17 55 26 596.3 0 26 56 24 596.3 0 24 57 17 596.3 0 17 58 13 596.3 0 13 59 13 596.3 0 13 60 18 596.3 0 18 61 20 596.3 0 20 62 11 596.3 0 11 63 19 596.3 0 19 64 29 596.3 0 29 65 34 596.3 0 34 66 30 596.3 0 30 67 14 596.3 0 14 68 36 596.3 0 36 69 23 596.3 0 23 70 40 596.3 0 40 71 69 596.3 0 69 72 825 596.3 0 825 73 90 596.3 0 90 74 674 596.3 0 674 75 75 596.3 0 75 76 97 596.3 0 97 77 32 596.3 0 32 78 91 596.3 0 91 79 149 596.3 0 149 80 243 596.3 0 243 81 151 596.3 0 151 82 167 596.3 0 167 83 284 596.3 0 284 84 186 596.3 0 186 85 633 596.3 0 633 86 90 596.3 0 90 87 77 596.3 0 77 88 104 596.3 0 104 89 88 596.3 0 88 90 68 596.3 0 68 91 40 596.3 0 40 92 73 596.3 0 73 93 59 596.3 0 59 94 40 596.3 0 40 95 69 596.3 0 69 96 49 596.3 0 49 97 40 596.3 0 40 98 44 596.3 0 44 99 61 596.3 0 61 100 47 596.3 0 47 101 50 596.3 0 50 102 96 596.3 0 96 103 76 596.3 0 76 104 75 596.3 0 75 105 94 596.3 0 94 106 154 596.3 0 154 107 1153 596.3 0 1153 108 1040 596.3 0 1040 109 1586 596.3 0 1586 110 88 596.3 0 88 111 78 596.3 0 78 112 78 596.3 0 78 113 92 596.3 0 92 114 72 596.3 0 72 115 84 596.3 0 84 116 82 596.3 0 82 117 121 596.3 0 121 118 127 596.3 0 127 119 160 596.3 0 160 120 561 596.3 0 561 121 476 596.3 0 476 122 489 596.3 0 489 123 241 596.3 0 241 124 179 596.3 0 179 125 212 596.3 0 212 126 98 596.3 0 98 127 100 596.3 0 100 128 110 596.3 0 110 129 73 596.3 0 73 130 95 596.3 0 95 131 76 596.3 0 76 132 72 596.3 0 72 133 70 596.3 0 70 134 60 596.3 0 60 135 68 596.3 0 68 136 92 596.3 0 92 137 157 596.3 0 157 138 117 596.3 0 117 139 148 596.3 0 148 140 110 596.3 0 110 141 128 596.3 0 128 142 144 596.3 0 144 143 158 596.3 0 158 144 99 596.3 0 99 145 428 596.3 0 428 146 152 596.3 0 152 147 124 596.3 0 124 148 100 596.3 0 100 149 117 596.3 0 117 150 101 596.3 0 101 151 186 596.3 0 186 152 107 596.3 0 107 153 94 596.3 0 94 154 96 596.3 0 96 155 97 596.3 0 97 156 103 596.3 0 103 157 88 596.3 0 88 158 96 596.3 0 96 159 113 596.3 0 113 160 103 596.3 0 103 161 97 596.3 0 97 162 110 596.3 0 110 163 117 596.3 0 117 164 157 596.3 0 157 165 208 596.3 0 208 166 2667 596.3 0 2667 167 1741 596.3 0 1741 168 2169 596.3 0 2169 169 178 596.3 0 178 170 639 596.3 0 639 171 817 596.3 0 817 172 694 596.3 0 694 173 161 596.3 0 161 174 185 596.3 0 185 175 121 596.3 0 121 176 168 596.3 0 168 177 160 596.3 0 160 178 158 596.3 0 158 179 598 596.3 0 598 180 3131 596.3 0 3131 181 6090 596.3 0 6090 182 1245 596.3 0 1245 183 1107 596.3 0 1107 184 1922 596.3 0 1922 185 3282 596.3 0 3282 186 2677 596.3 0 2677 187 113 596.3 0 113 188 108 596.3 0 108 189 128 596.3 0 128 190 72 596.3 0 72 191 89 596.3 0 89 192 81 596.3 0 81 193 80 596.3 0 80 194 74 596.3 0 74 195 80 596.3 0 80 196 119 596.3 0 119 197 97 596.3 0 97 198 199 596.3 0 199 199 268 596.3 0 268 200 256 596.3 0 256 201 173 596.3 0 173 202 106 596.3 0 106 203 136 596.3 0 136 204 262 596.3 0 262 205 504 596.3 0 504 206 969 596.3 0 969 207 264 596.3 0 264 208 93 596.3 0 93 209 133 596.3 0 133 210 114 596.3 0 114 211 197 596.3 0 197 212 210 596.3 0 210 213 1291 596.3 0 1291 214 5350 596.3 0 5350 215 2919 596.3 0 2919 216 305 596.3 0 305 217 96 596.3 0 96 218 112 596.3 0 112 219 129 596.3 0 129 220 159 596.3 0 159 221 86 596.3 0 86 222 70 596.3 0 70 223 70 596.3 0 70 224 97 596.3 0 97 225 219 596.3 0 219 226 176 596.3 0 176 227 293 596.3 0 293 228 399 596.3 0 399 229 251 596.3 0 251 230 225 596.3 0 225 231 86 596.3 0 86 232 68 596.3 0 68 233 77 596.3 0 77 234 64 596.3 0 64 235 56 596.3 0 56 236 52 596.3 0 52 237 117 596.3 0 117 238 146 596.3 0 146 239 128 596.3 0 128 240 200 596.3 0 200 241 82 596.3 0 82 242 131 596.3 0 131 243 195 596.3 0 195 244 194 596.3 0 194 245 104 596.3 0 104 246 140 596.3 0 140 247 105 596.3 0 105 248 141 596.3 0 141 249 102 596.3 0 102 250 115 596.3 0 115 251 79 596.3 0 79 252 96 596.3 0 96 253 120 596.3 0 120 254 191 596.3 0 191 255 182 596.3 0 182 256 195 596.3 0 195 257 200 596.3 0 200 258 91 596.3 0 91 259 80 596.3 0 80 260 133 596.3 0 133 261 174 596.3 0 174 262 301 596.3 0 301 263 394 596.3 0 394 264 356 596.3 0 356 265 174 596.3 0 174 266 231 596.3 0 231 267 2728 596.3 0 2728 268 4819 596.3 0 4819 269 3415 596.3 0 3415 270 196 596.3 0 196 271 61 596.3 0 61 272 64 596.3 0 64 273 83 596.3 0 83 274 86 596.3 0 86 275 96 596.3 0 96 276 123 596.3 0 123 277 152 596.3 0 152 278 89 596.3 0 89 279 106 596.3 0 106 280 336 596.3 0 336 281 334 596.3 0 334 282 329 596.3 0 329 283 105 596.3 0 105 284 50 596.3 0 50 285 63 596.3 0 63 286 74 596.3 0 74 287 89 596.3 0 89 288 83 596.3 0 83 289 86 596.3 0 86 290 75 596.3 0 75 291 83 596.3 0 83 292 61 596.3 0 61 293 83 596.3 0 83 294 85 596.3 0 85 295 98 596.3 0 98 296 76 596.3 0 76 297 70 596.3 0 70 298 81 596.3 0 81 299 137 596.3 0 137 300 142 596.3 0 142 301 158 596.3 0 158 Finished in 2.60 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 71,445 Reads with adapters: 10,244 (14.3%) Reads that were too short: 166 (0.2%) Reads written (passing filters): 10,078 (14.1%) Total basepairs processed: 13,666,778 bp Total written (filtered): 2,758,916 bp (20.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10244 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 8 0.0 3 0 0 7 1 16 74 0.0 3 0 70 4 17 9294 0.0 3 9092 192 9 1 18 50 0.0 3 25 22 3 19 16 0.0 3 2 5 7 2 20 555 0.0 3 540 13 0 2 21 24 0.0 3 0 1 0 23 24 9 0.0 3 0 0 1 8 26 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 3 0.0 3 0 0 0 3 46 4 0.0 3 0 0 0 4 47 85 0.0 3 0 0 0 85 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 5 0.0 3 0 0 0 5 62 1 0.0 3 0 0 0 1 66 16 0.0 3 0 0 0 16 71 4 0.0 3 0 0 0 4 72 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 82 1 0.0 3 1 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 97 38 0.0 3 0 0 1 37 102 2 0.0 3 0 0 1 1 103 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 116 2 0.0 3 0 0 0 2 117 4 0.0 3 0 0 0 4 118 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 6 0.0 3 0 0 0 6 130 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 165 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.36 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 10,078 Reads with adapters: 374 (3.7%) Reads written (passing filters): 10,078 (100.0%) Total basepairs processed: 2,758,916 bp Total written (filtered): 2,757,779 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 374 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 78.1% C: 8.0% G: 11.2% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 361 157.5 0 361 4 11 39.4 0 11 5 2 9.8 0 2