This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.17 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 56,934 (2.3%) Reads that were too short: 17,314 (0.7%) Reads written (passing filters): 39,620 (1.6%) Total basepairs processed: 735,190,694 bp Total written (filtered): 7,106,830 bp (1.0%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 56934 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 189 596.3 0 189 7 654 596.3 0 654 8 2776 596.3 0 2776 9 2397 596.3 0 2397 10 30 596.3 0 30 11 42 596.3 0 42 12 29 596.3 0 29 13 17 596.3 0 17 14 24 596.3 0 24 15 24 596.3 0 24 16 22 596.3 0 22 17 19 596.3 0 19 18 27 596.3 0 27 19 22 596.3 0 22 20 32 596.3 0 32 21 33 596.3 0 33 22 20 596.3 0 20 23 24 596.3 0 24 24 22 596.3 0 22 25 23 596.3 0 23 26 26 596.3 0 26 27 33 596.3 0 33 28 26 596.3 0 26 29 48 596.3 0 48 30 24 596.3 0 24 31 29 596.3 0 29 32 8 596.3 0 8 33 20 596.3 0 20 34 31 596.3 0 31 35 30 596.3 0 30 36 42 596.3 0 42 37 24 596.3 0 24 38 30 596.3 0 30 39 33 596.3 0 33 40 35 596.3 0 35 41 31 596.3 0 31 42 20 596.3 0 20 43 30 596.3 0 30 44 28 596.3 0 28 45 32 596.3 0 32 46 55 596.3 0 55 47 22 596.3 0 22 48 28 596.3 0 28 49 43 596.3 0 43 50 34 596.3 0 34 51 27 596.3 0 27 52 27 596.3 0 27 53 91 596.3 0 91 54 53 596.3 0 53 55 114 596.3 0 114 56 31 596.3 0 31 57 29 596.3 0 29 58 53 596.3 0 53 59 49 596.3 0 49 60 31 596.3 0 31 61 54 596.3 0 54 62 38 596.3 0 38 63 123 596.3 0 123 64 92 596.3 0 92 65 126 596.3 0 126 66 29 596.3 0 29 67 50 596.3 0 50 68 85 596.3 0 85 69 124 596.3 0 124 70 50 596.3 0 50 71 75 596.3 0 75 72 406 596.3 0 406 73 1545 596.3 0 1545 74 1796 596.3 0 1796 75 1274 596.3 0 1274 76 25 596.3 0 25 77 36 596.3 0 36 78 33 596.3 0 33 79 36 596.3 0 36 80 31 596.3 0 31 81 10 596.3 0 10 82 24 596.3 0 24 83 27 596.3 0 27 84 36 596.3 0 36 85 41 596.3 0 41 86 61 596.3 0 61 87 121 596.3 0 121 88 315 596.3 0 315 89 70 596.3 0 70 90 38 596.3 0 38 91 63 596.3 0 63 92 41 596.3 0 41 93 37 596.3 0 37 94 40 596.3 0 40 95 39 596.3 0 39 96 34 596.3 0 34 97 24 596.3 0 24 98 27 596.3 0 27 99 37 596.3 0 37 100 60 596.3 0 60 101 54 596.3 0 54 102 28 596.3 0 28 103 25 596.3 0 25 104 32 596.3 0 32 105 79 596.3 0 79 106 35 596.3 0 35 107 50 596.3 0 50 108 72 596.3 0 72 109 97 596.3 0 97 110 55 596.3 0 55 111 28 596.3 0 28 112 22 596.3 0 22 113 27 596.3 0 27 114 31 596.3 0 31 115 41 596.3 0 41 116 28 596.3 0 28 117 26 596.3 0 26 118 33 596.3 0 33 119 25 596.3 0 25 120 28 596.3 0 28 121 44 596.3 0 44 122 43 596.3 0 43 123 22 596.3 0 22 124 27 596.3 0 27 125 31 596.3 0 31 126 35 596.3 0 35 127 37 596.3 0 37 128 68 596.3 0 68 129 64 596.3 0 64 130 88 596.3 0 88 131 52 596.3 0 52 132 52 596.3 0 52 133 52 596.3 0 52 134 35 596.3 0 35 135 73 596.3 0 73 136 297 596.3 0 297 137 157 596.3 0 157 138 63 596.3 0 63 139 39 596.3 0 39 140 37 596.3 0 37 141 87 596.3 0 87 142 93 596.3 0 93 143 102 596.3 0 102 144 64 596.3 0 64 145 129 596.3 0 129 146 1133 596.3 0 1133 147 282 596.3 0 282 148 405 596.3 0 405 149 113 596.3 0 113 150 143 596.3 0 143 151 82 596.3 0 82 152 84 596.3 0 84 153 85 596.3 0 85 154 220 596.3 0 220 155 166 596.3 0 166 156 229 596.3 0 229 157 285 596.3 0 285 158 628 596.3 0 628 159 390 596.3 0 390 160 629 596.3 0 629 161 338 596.3 0 338 162 260 596.3 0 260 163 95 596.3 0 95 164 53 596.3 0 53 165 81 596.3 0 81 166 148 596.3 0 148 167 257 596.3 0 257 168 149 596.3 0 149 169 146 596.3 0 146 170 161 596.3 0 161 171 484 596.3 0 484 172 153 596.3 0 153 173 267 596.3 0 267 174 2712 596.3 0 2712 175 1730 596.3 0 1730 176 3126 596.3 0 3126 177 209 596.3 0 209 178 76 596.3 0 76 179 56 596.3 0 56 180 150 596.3 0 150 181 695 596.3 0 695 182 360 596.3 0 360 183 105 596.3 0 105 184 88 596.3 0 88 185 190 596.3 0 190 186 386 596.3 0 386 187 2144 596.3 0 2144 188 886 596.3 0 886 189 1064 596.3 0 1064 190 56 596.3 0 56 191 56 596.3 0 56 192 64 596.3 0 64 193 41 596.3 0 41 194 66 596.3 0 66 195 84 596.3 0 84 196 67 596.3 0 67 197 52 596.3 0 52 198 43 596.3 0 43 199 49 596.3 0 49 200 61 596.3 0 61 201 65 596.3 0 65 202 94 596.3 0 94 203 94 596.3 0 94 204 145 596.3 0 145 205 152 596.3 0 152 206 147 596.3 0 147 207 74 596.3 0 74 208 56 596.3 0 56 209 71 596.3 0 71 210 46 596.3 0 46 211 40 596.3 0 40 212 42 596.3 0 42 213 60 596.3 0 60 214 69 596.3 0 69 215 78 596.3 0 78 216 71 596.3 0 71 217 78 596.3 0 78 218 71 596.3 0 71 219 67 596.3 0 67 220 87 596.3 0 87 221 68 596.3 0 68 222 66 596.3 0 66 223 87 596.3 0 87 224 73 596.3 0 73 225 73 596.3 0 73 226 60 596.3 0 60 227 214 596.3 0 214 228 138 596.3 0 138 229 310 596.3 0 310 230 70 596.3 0 70 231 103 596.3 0 103 232 128 596.3 0 128 233 112 596.3 0 112 234 86 596.3 0 86 235 80 596.3 0 80 236 121 596.3 0 121 237 168 596.3 0 168 238 149 596.3 0 149 239 204 596.3 0 204 240 116 596.3 0 116 241 96 596.3 0 96 242 86 596.3 0 86 243 80 596.3 0 80 244 99 596.3 0 99 245 66 596.3 0 66 246 95 596.3 0 95 247 117 596.3 0 117 248 259 596.3 0 259 249 304 596.3 0 304 250 217 596.3 0 217 251 416 596.3 0 416 252 299 596.3 0 299 253 443 596.3 0 443 254 161 596.3 0 161 255 148 596.3 0 148 256 105 596.3 0 105 257 74 596.3 0 74 258 76 596.3 0 76 259 127 596.3 0 127 260 281 596.3 0 281 261 539 596.3 0 539 262 633 596.3 0 633 263 455 596.3 0 455 264 254 596.3 0 254 265 77 596.3 0 77 266 68 596.3 0 68 267 73 596.3 0 73 268 166 596.3 0 166 269 214 596.3 0 214 270 162 596.3 0 162 271 122 596.3 0 122 272 195 596.3 0 195 273 142 596.3 0 142 274 148 596.3 0 148 275 86 596.3 0 86 276 79 596.3 0 79 277 151 596.3 0 151 278 216 596.3 0 216 279 278 596.3 0 278 280 188 596.3 0 188 281 412 596.3 0 412 282 403 596.3 0 403 283 343 596.3 0 343 284 143 596.3 0 143 285 293 596.3 0 293 286 225 596.3 0 225 287 127 596.3 0 127 288 112 596.3 0 112 289 105 596.3 0 105 290 214 596.3 0 214 291 329 596.3 0 329 292 310 596.3 0 310 293 252 596.3 0 252 294 180 596.3 0 180 295 290 596.3 0 290 296 178 596.3 0 178 297 327 596.3 0 327 298 316 596.3 0 316 299 340 596.3 0 340 300 374 596.3 0 374 301 278 596.3 0 278 Finished in 1.56 s (39 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 39,620 Reads with adapters: 2,708 (6.8%) Reads that were too short: 34 (0.1%) Reads written (passing filters): 2,674 (6.7%) Total basepairs processed: 7,106,830 bp Total written (filtered): 723,122 bp (10.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2708 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 5 0.0 3 0 0 4 0 1 19 33 0.0 3 0 27 4 2 20 2477 0.0 4 2313 122 33 5 4 21 8 0.0 4 5 3 22 5 0.0 4 1 1 1 1 1 23 106 0.0 4 90 3 0 11 2 48 1 0.0 4 0 0 0 0 1 55 2 0.0 4 0 0 0 0 2 56 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 99 2 0.0 4 0 0 0 0 2 106 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 127 34 0.0 4 0 0 0 0 34 129 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 155 18 0.0 4 0 0 0 0 18 157 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.10 s (37 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 2,674 Reads with adapters: 663 (24.8%) Reads written (passing filters): 2,674 (100.0%) Total basepairs processed: 723,122 bp Total written (filtered): 715,604 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 663 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.0% C: 8.4% G: 86.9% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 7 1 0.2 0 1 9 183 0.0 0 15 168 10 342 0.0 1 18 324 11 130 0.0 1 4 126 12 1 0.0 1 0 1 14 1 0.0 1 0 1 150 1 0.0 1 1 151 1 0.0 1 1 208 2 0.0 1 2 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.