This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.84 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 34,854 (1.4%) Reads that were too short: 7,038 (0.3%) Reads written (passing filters): 27,816 (1.1%) Total basepairs processed: 735,190,694 bp Total written (filtered): 5,727,051 bp (0.8%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 34854 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 211 596.3 0 211 7 526 596.3 0 526 8 2405 596.3 0 2405 9 2908 596.3 0 2908 10 20 596.3 0 20 11 21 596.3 0 21 12 13 596.3 0 13 13 20 596.3 0 20 14 23 596.3 0 23 15 26 596.3 0 26 16 23 596.3 0 23 17 21 596.3 0 21 18 14 596.3 0 14 19 16 596.3 0 16 20 13 596.3 0 13 21 29 596.3 0 29 22 22 596.3 0 22 23 15 596.3 0 15 24 25 596.3 0 25 25 30 596.3 0 30 26 14 596.3 0 14 27 18 596.3 0 18 28 18 596.3 0 18 29 21 596.3 0 21 30 21 596.3 0 21 31 22 596.3 0 22 32 21 596.3 0 21 33 19 596.3 0 19 34 23 596.3 0 23 35 28 596.3 0 28 36 152 596.3 0 152 37 34 596.3 0 34 38 24 596.3 0 24 39 38 596.3 0 38 40 24 596.3 0 24 41 24 596.3 0 24 42 14 596.3 0 14 43 22 596.3 0 22 44 17 596.3 0 17 45 33 596.3 0 33 46 28 596.3 0 28 47 25 596.3 0 25 48 18 596.3 0 18 49 29 596.3 0 29 50 22 596.3 0 22 51 24 596.3 0 24 52 21 596.3 0 21 53 42 596.3 0 42 54 26 596.3 0 26 55 65 596.3 0 65 56 735 596.3 0 735 57 67 596.3 0 67 58 40 596.3 0 40 59 31 596.3 0 31 60 39 596.3 0 39 61 35 596.3 0 35 62 38 596.3 0 38 63 31 596.3 0 31 64 35 596.3 0 35 65 34 596.3 0 34 66 22 596.3 0 22 67 55 596.3 0 55 68 73 596.3 0 73 69 71 596.3 0 71 70 43 596.3 0 43 71 108 596.3 0 108 72 537 596.3 0 537 73 2117 596.3 0 2117 74 2599 596.3 0 2599 75 1589 596.3 0 1589 76 22 596.3 0 22 77 21 596.3 0 21 78 15 596.3 0 15 79 22 596.3 0 22 80 16 596.3 0 16 81 12 596.3 0 12 82 21 596.3 0 21 83 19 596.3 0 19 84 30 596.3 0 30 85 30 596.3 0 30 86 34 596.3 0 34 87 37 596.3 0 37 88 60 596.3 0 60 89 44 596.3 0 44 90 31 596.3 0 31 91 27 596.3 0 27 92 34 596.3 0 34 93 43 596.3 0 43 94 67 596.3 0 67 95 17 596.3 0 17 96 18 596.3 0 18 97 20 596.3 0 20 98 28 596.3 0 28 99 31 596.3 0 31 100 22 596.3 0 22 101 21 596.3 0 21 102 22 596.3 0 22 103 25 596.3 0 25 104 27 596.3 0 27 105 34 596.3 0 34 106 26 596.3 0 26 107 16 596.3 0 16 108 26 596.3 0 26 109 48 596.3 0 48 110 32 596.3 0 32 111 22 596.3 0 22 112 20 596.3 0 20 113 15 596.3 0 15 114 31 596.3 0 31 115 33 596.3 0 33 116 23 596.3 0 23 117 14 596.3 0 14 118 16 596.3 0 16 119 29 596.3 0 29 120 22 596.3 0 22 121 23 596.3 0 23 122 14 596.3 0 14 123 18 596.3 0 18 124 17 596.3 0 17 125 16 596.3 0 16 126 18 596.3 0 18 127 22 596.3 0 22 128 37 596.3 0 37 129 42 596.3 0 42 130 34 596.3 0 34 131 28 596.3 0 28 132 18 596.3 0 18 133 43 596.3 0 43 134 20 596.3 0 20 135 40 596.3 0 40 136 285 596.3 0 285 137 65 596.3 0 65 138 30 596.3 0 30 139 18 596.3 0 18 140 29 596.3 0 29 141 31 596.3 0 31 142 37 596.3 0 37 143 68 596.3 0 68 144 39 596.3 0 39 145 81 596.3 0 81 146 1190 596.3 0 1190 147 123 596.3 0 123 148 101 596.3 0 101 149 82 596.3 0 82 150 105 596.3 0 105 151 44 596.3 0 44 152 39 596.3 0 39 153 41 596.3 0 41 154 233 596.3 0 233 155 62 596.3 0 62 156 112 596.3 0 112 157 71 596.3 0 71 158 311 596.3 0 311 159 150 596.3 0 150 160 494 596.3 0 494 161 165 596.3 0 165 162 69 596.3 0 69 163 63 596.3 0 63 164 32 596.3 0 32 165 18 596.3 0 18 166 38 596.3 0 38 167 57 596.3 0 57 168 42 596.3 0 42 169 42 596.3 0 42 170 77 596.3 0 77 171 462 596.3 0 462 172 56 596.3 0 56 173 89 596.3 0 89 174 884 596.3 0 884 175 494 596.3 0 494 176 1155 596.3 0 1155 177 48 596.3 0 48 178 34 596.3 0 34 179 22 596.3 0 22 180 77 596.3 0 77 181 528 596.3 0 528 182 204 596.3 0 204 183 45 596.3 0 45 184 45 596.3 0 45 185 48 596.3 0 48 186 108 596.3 0 108 187 770 596.3 0 770 188 373 596.3 0 373 189 384 596.3 0 384 190 37 596.3 0 37 191 42 596.3 0 42 192 27 596.3 0 27 193 24 596.3 0 24 194 37 596.3 0 37 195 42 596.3 0 42 196 25 596.3 0 25 197 31 596.3 0 31 198 25 596.3 0 25 199 29 596.3 0 29 200 25 596.3 0 25 201 22 596.3 0 22 202 25 596.3 0 25 203 34 596.3 0 34 204 67 596.3 0 67 205 60 596.3 0 60 206 54 596.3 0 54 207 37 596.3 0 37 208 19 596.3 0 19 209 27 596.3 0 27 210 24 596.3 0 24 211 22 596.3 0 22 212 17 596.3 0 17 213 15 596.3 0 15 214 27 596.3 0 27 215 31 596.3 0 31 216 23 596.3 0 23 217 42 596.3 0 42 218 28 596.3 0 28 219 37 596.3 0 37 220 30 596.3 0 30 221 22 596.3 0 22 222 36 596.3 0 36 223 39 596.3 0 39 224 28 596.3 0 28 225 29 596.3 0 29 226 25 596.3 0 25 227 108 596.3 0 108 228 76 596.3 0 76 229 200 596.3 0 200 230 34 596.3 0 34 231 53 596.3 0 53 232 46 596.3 0 46 233 43 596.3 0 43 234 37 596.3 0 37 235 30 596.3 0 30 236 40 596.3 0 40 237 69 596.3 0 69 238 72 596.3 0 72 239 83 596.3 0 83 240 31 596.3 0 31 241 39 596.3 0 39 242 40 596.3 0 40 243 28 596.3 0 28 244 32 596.3 0 32 245 32 596.3 0 32 246 45 596.3 0 45 247 33 596.3 0 33 248 76 596.3 0 76 249 89 596.3 0 89 250 71 596.3 0 71 251 172 596.3 0 172 252 104 596.3 0 104 253 187 596.3 0 187 254 61 596.3 0 61 255 53 596.3 0 53 256 38 596.3 0 38 257 30 596.3 0 30 258 36 596.3 0 36 259 56 596.3 0 56 260 116 596.3 0 116 261 176 596.3 0 176 262 212 596.3 0 212 263 149 596.3 0 149 264 96 596.3 0 96 265 29 596.3 0 29 266 37 596.3 0 37 267 33 596.3 0 33 268 73 596.3 0 73 269 61 596.3 0 61 270 65 596.3 0 65 271 45 596.3 0 45 272 69 596.3 0 69 273 83 596.3 0 83 274 68 596.3 0 68 275 58 596.3 0 58 276 61 596.3 0 61 277 73 596.3 0 73 278 69 596.3 0 69 279 105 596.3 0 105 280 68 596.3 0 68 281 72 596.3 0 72 282 113 596.3 0 113 283 166 596.3 0 166 284 64 596.3 0 64 285 181 596.3 0 181 286 80 596.3 0 80 287 66 596.3 0 66 288 61 596.3 0 61 289 48 596.3 0 48 290 69 596.3 0 69 291 93 596.3 0 93 292 88 596.3 0 88 293 73 596.3 0 73 294 98 596.3 0 98 295 246 596.3 0 246 296 90 596.3 0 90 297 140 596.3 0 140 298 91 596.3 0 91 299 101 596.3 0 101 300 230 596.3 0 230 301 180 596.3 0 180 Finished in 1.06 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 27,816 Reads with adapters: 2,830 (10.2%) Reads that were too short: 41 (0.1%) Reads written (passing filters): 2,789 (10.0%) Total basepairs processed: 5,727,051 bp Total written (filtered): 766,066 bp (13.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2830 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 16 17 0.0 3 0 16 0 1 17 2418 0.0 3 2366 49 3 18 13 0.0 3 1 12 19 311 0.0 3 305 4 2 20 7 0.0 3 1 3 0 3 26 2 0.0 3 0 0 1 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 46 9 0.0 3 0 0 0 9 49 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 106 19 0.0 3 0 0 0 19 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.09 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 2,789 Reads with adapters: 92 (3.3%) Reads written (passing filters): 2,789 (100.0%) Total basepairs processed: 766,066 bp Total written (filtered): 765,708 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 92 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 7.6% C: 1.1% G: 89.1% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 10 43.6 0 10 4 82 10.9 0 82 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.