This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.38 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 47,099 (1.9%) Reads that were too short: 19,457 (0.8%) Reads written (passing filters): 27,642 (1.1%) Total basepairs processed: 735,190,694 bp Total written (filtered): 4,700,213 bp (0.6%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 47099 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 83 596.3 0 83 7 1931 596.3 0 1931 8 1598 596.3 0 1598 9 15 596.3 0 15 10 21 596.3 0 21 11 82 596.3 0 82 12 28 596.3 0 28 13 187 596.3 0 187 14 113 596.3 0 113 15 248 596.3 0 248 16 45 596.3 0 45 17 8 596.3 0 8 18 4 596.3 0 4 19 7 596.3 0 7 20 8 596.3 0 8 21 14 596.3 0 14 22 11 596.3 0 11 23 12 596.3 0 12 24 30 596.3 0 30 25 76 596.3 0 76 26 59 596.3 0 59 27 107 596.3 0 107 28 8 596.3 0 8 29 10 596.3 0 10 30 10 596.3 0 10 31 12 596.3 0 12 32 11 596.3 0 11 33 8 596.3 0 8 34 10 596.3 0 10 35 20 596.3 0 20 36 9 596.3 0 9 37 9 596.3 0 9 38 13 596.3 0 13 39 11 596.3 0 11 40 14 596.3 0 14 41 7 596.3 0 7 42 15 596.3 0 15 43 22 596.3 0 22 44 6 596.3 0 6 45 7 596.3 0 7 46 8 596.3 0 8 47 10 596.3 0 10 48 15 596.3 0 15 49 12 596.3 0 12 50 20 596.3 0 20 51 6 596.3 0 6 52 11 596.3 0 11 53 19 596.3 0 19 54 25 596.3 0 25 55 22 596.3 0 22 56 24 596.3 0 24 57 27 596.3 0 27 58 18 596.3 0 18 59 50 596.3 0 50 60 55 596.3 0 55 61 90 596.3 0 90 62 76 596.3 0 76 63 12 596.3 0 12 64 10 596.3 0 10 65 11 596.3 0 11 66 19 596.3 0 19 67 10 596.3 0 10 68 15 596.3 0 15 69 85 596.3 0 85 70 15 596.3 0 15 71 17 596.3 0 17 72 22 596.3 0 22 73 17 596.3 0 17 74 23 596.3 0 23 75 20 596.3 0 20 76 15 596.3 0 15 77 27 596.3 0 27 78 50 596.3 0 50 79 55 596.3 0 55 80 109 596.3 0 109 81 70 596.3 0 70 82 534 596.3 0 534 83 326 596.3 0 326 84 398 596.3 0 398 85 13 596.3 0 13 86 16 596.3 0 16 87 17 596.3 0 17 88 19 596.3 0 19 89 28 596.3 0 28 90 12 596.3 0 12 91 13 596.3 0 13 92 12 596.3 0 12 93 31 596.3 0 31 94 17 596.3 0 17 95 22 596.3 0 22 96 37 596.3 0 37 97 41 596.3 0 41 98 23 596.3 0 23 99 35 596.3 0 35 100 30 596.3 0 30 101 13 596.3 0 13 102 41 596.3 0 41 103 45 596.3 0 45 104 59 596.3 0 59 105 41 596.3 0 41 106 108 596.3 0 108 107 44 596.3 0 44 108 24 596.3 0 24 109 33 596.3 0 33 110 286 596.3 0 286 111 121 596.3 0 121 112 52 596.3 0 52 113 72 596.3 0 72 114 30 596.3 0 30 115 44 596.3 0 44 116 32 596.3 0 32 117 27 596.3 0 27 118 23 596.3 0 23 119 57 596.3 0 57 120 62 596.3 0 62 121 38 596.3 0 38 122 151 596.3 0 151 123 171 596.3 0 171 124 87 596.3 0 87 125 127 596.3 0 127 126 161 596.3 0 161 127 61 596.3 0 61 128 47 596.3 0 47 129 30 596.3 0 30 130 29 596.3 0 29 131 36 596.3 0 36 132 40 596.3 0 40 133 42 596.3 0 42 134 66 596.3 0 66 135 38 596.3 0 38 136 63 596.3 0 63 137 136 596.3 0 136 138 179 596.3 0 179 139 175 596.3 0 175 140 133 596.3 0 133 141 84 596.3 0 84 142 111 596.3 0 111 143 40 596.3 0 40 144 37 596.3 0 37 145 108 596.3 0 108 146 141 596.3 0 141 147 84 596.3 0 84 148 77 596.3 0 77 149 37 596.3 0 37 150 50 596.3 0 50 151 56 596.3 0 56 152 52 596.3 0 52 153 86 596.3 0 86 154 50 596.3 0 50 155 103 596.3 0 103 156 143 596.3 0 143 157 144 596.3 0 144 158 159 596.3 0 159 159 191 596.3 0 191 160 867 596.3 0 867 161 1356 596.3 0 1356 162 1586 596.3 0 1586 163 543 596.3 0 543 164 119 596.3 0 119 165 62 596.3 0 62 166 82 596.3 0 82 167 63 596.3 0 63 168 74 596.3 0 74 169 80 596.3 0 80 170 87 596.3 0 87 171 37 596.3 0 37 172 62 596.3 0 62 173 71 596.3 0 71 174 122 596.3 0 122 175 106 596.3 0 106 176 93 596.3 0 93 177 95 596.3 0 95 178 85 596.3 0 85 179 106 596.3 0 106 180 345 596.3 0 345 181 758 596.3 0 758 182 835 596.3 0 835 183 526 596.3 0 526 184 156 596.3 0 156 185 164 596.3 0 164 186 242 596.3 0 242 187 2783 596.3 0 2783 188 1072 596.3 0 1072 189 1928 596.3 0 1928 190 67 596.3 0 67 191 69 596.3 0 69 192 35 596.3 0 35 193 41 596.3 0 41 194 50 596.3 0 50 195 52 596.3 0 52 196 78 596.3 0 78 197 65 596.3 0 65 198 59 596.3 0 59 199 102 596.3 0 102 200 74 596.3 0 74 201 65 596.3 0 65 202 92 596.3 0 92 203 152 596.3 0 152 204 178 596.3 0 178 205 232 596.3 0 232 206 425 596.3 0 425 207 255 596.3 0 255 208 92 596.3 0 92 209 62 596.3 0 62 210 389 596.3 0 389 211 792 596.3 0 792 212 1062 596.3 0 1062 213 366 596.3 0 366 214 163 596.3 0 163 215 91 596.3 0 91 216 95 596.3 0 95 217 71 596.3 0 71 218 62 596.3 0 62 219 214 596.3 0 214 220 287 596.3 0 287 221 333 596.3 0 333 222 81 596.3 0 81 223 55 596.3 0 55 224 127 596.3 0 127 225 131 596.3 0 131 226 136 596.3 0 136 227 138 596.3 0 138 228 116 596.3 0 116 229 149 596.3 0 149 230 763 596.3 0 763 231 925 596.3 0 925 232 998 596.3 0 998 233 93 596.3 0 93 234 37 596.3 0 37 235 40 596.3 0 40 236 40 596.3 0 40 237 33 596.3 0 33 238 34 596.3 0 34 239 65 596.3 0 65 240 86 596.3 0 86 241 99 596.3 0 99 242 52 596.3 0 52 243 43 596.3 0 43 244 39 596.3 0 39 245 48 596.3 0 48 246 37 596.3 0 37 247 52 596.3 0 52 248 30 596.3 0 30 249 24 596.3 0 24 250 32 596.3 0 32 251 46 596.3 0 46 252 58 596.3 0 58 253 105 596.3 0 105 254 132 596.3 0 132 255 116 596.3 0 116 256 110 596.3 0 110 257 69 596.3 0 69 258 84 596.3 0 84 259 78 596.3 0 78 260 95 596.3 0 95 261 76 596.3 0 76 262 59 596.3 0 59 263 107 596.3 0 107 264 109 596.3 0 109 265 103 596.3 0 103 266 70 596.3 0 70 267 74 596.3 0 74 268 144 596.3 0 144 269 128 596.3 0 128 270 56 596.3 0 56 271 66 596.3 0 66 272 83 596.3 0 83 273 109 596.3 0 109 274 216 596.3 0 216 275 424 596.3 0 424 276 550 596.3 0 550 277 476 596.3 0 476 278 199 596.3 0 199 279 303 596.3 0 303 280 335 596.3 0 335 281 203 596.3 0 203 282 121 596.3 0 121 283 244 596.3 0 244 284 260 596.3 0 260 285 264 596.3 0 264 286 117 596.3 0 117 287 200 596.3 0 200 288 280 596.3 0 280 289 621 596.3 0 621 290 282 596.3 0 282 291 110 596.3 0 110 292 67 596.3 0 67 293 94 596.3 0 94 294 111 596.3 0 111 295 125 596.3 0 125 296 103 596.3 0 103 297 113 596.3 0 113 298 184 596.3 0 184 299 80 596.3 0 80 300 354 596.3 0 354 301 1028 596.3 0 1028 Finished in 1.04 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 27,642 Reads with adapters: 1,695 (6.1%) Reads that were too short: 15 (0.1%) Reads written (passing filters): 1,680 (6.1%) Total basepairs processed: 4,700,213 bp Total written (filtered): 455,835 bp (9.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1695 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 28 0.0 3 0 24 3 0 1 20 1611 0.0 4 1509 85 12 4 1 21 7 0.0 4 2 4 1 23 11 0.0 4 4 0 0 3 4 25 1 0.0 4 1 30 1 0.0 4 0 0 0 0 1 38 2 0.0 4 0 0 0 0 2 52 1 0.0 4 0 0 0 1 55 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 1 110 2 0.0 4 1 0 0 0 1 111 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 15 0.0 4 0 0 0 0 15 128 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 1,680 Reads with adapters: 312 (18.6%) Reads written (passing filters): 1,680 (100.0%) Total basepairs processed: 455,835 bp Total written (filtered): 452,224 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 312 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.2% C: 8.3% G: 87.2% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 26.2 0 1 4 1 6.6 0 1 9 2 0.0 0 1 1 10 67 0.0 1 8 59 11 235 0.0 1 11 224 12 3 0.0 1 0 3 13 2 0.0 1 0 2 269 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.