This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.64 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 31,117 (1.3%) Reads that were too short: 9,705 (0.4%) Reads written (passing filters): 21,412 (0.9%) Total basepairs processed: 735,190,694 bp Total written (filtered): 3,872,130 bp (0.5%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 31117 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 51 596.3 0 51 7 1685 596.3 0 1685 8 1987 596.3 0 1987 9 5 596.3 0 5 10 10 596.3 0 10 11 35 596.3 0 35 12 8 596.3 0 8 13 104 596.3 0 104 14 65 596.3 0 65 15 118 596.3 0 118 16 36 596.3 0 36 17 4 596.3 0 4 18 6 596.3 0 6 19 6 596.3 0 6 20 4 596.3 0 4 21 6 596.3 0 6 22 5 596.3 0 5 23 11 596.3 0 11 24 9 596.3 0 9 25 492 596.3 0 492 26 109 596.3 0 109 27 6 596.3 0 6 28 9 596.3 0 9 29 8 596.3 0 8 30 4 596.3 0 4 31 10 596.3 0 10 32 4 596.3 0 4 33 11 596.3 0 11 34 5 596.3 0 5 35 5 596.3 0 5 36 2 596.3 0 2 37 9 596.3 0 9 38 5 596.3 0 5 39 4 596.3 0 4 40 9 596.3 0 9 41 7 596.3 0 7 42 7 596.3 0 7 43 9 596.3 0 9 44 14 596.3 0 14 45 7 596.3 0 7 46 7 596.3 0 7 47 16 596.3 0 16 48 8 596.3 0 8 49 6 596.3 0 6 50 8 596.3 0 8 51 8 596.3 0 8 52 7 596.3 0 7 53 21 596.3 0 21 54 23 596.3 0 23 55 20 596.3 0 20 56 20 596.3 0 20 57 20 596.3 0 20 58 13 596.3 0 13 59 47 596.3 0 47 60 30 596.3 0 30 61 54 596.3 0 54 62 19 596.3 0 19 63 8 596.3 0 8 64 11 596.3 0 11 65 15 596.3 0 15 66 11 596.3 0 11 67 10 596.3 0 10 68 19 596.3 0 19 69 121 596.3 0 121 70 19 596.3 0 19 71 14 596.3 0 14 72 13 596.3 0 13 73 8 596.3 0 8 74 12 596.3 0 12 75 13 596.3 0 13 76 11 596.3 0 11 77 14 596.3 0 14 78 24 596.3 0 24 79 20 596.3 0 20 80 35 596.3 0 35 81 92 596.3 0 92 82 861 596.3 0 861 83 565 596.3 0 565 84 778 596.3 0 778 85 16 596.3 0 16 86 26 596.3 0 26 87 27 596.3 0 27 88 21 596.3 0 21 89 35 596.3 0 35 90 24 596.3 0 24 91 19 596.3 0 19 92 11 596.3 0 11 93 23 596.3 0 23 94 23 596.3 0 23 95 24 596.3 0 24 96 20 596.3 0 20 97 26 596.3 0 26 98 23 596.3 0 23 99 14 596.3 0 14 100 25 596.3 0 25 101 29 596.3 0 29 102 27 596.3 0 27 103 28 596.3 0 28 104 28 596.3 0 28 105 37 596.3 0 37 106 112 596.3 0 112 107 27 596.3 0 27 108 31 596.3 0 31 109 21 596.3 0 21 110 293 596.3 0 293 111 100 596.3 0 100 112 36 596.3 0 36 113 53 596.3 0 53 114 29 596.3 0 29 115 35 596.3 0 35 116 22 596.3 0 22 117 21 596.3 0 21 118 30 596.3 0 30 119 42 596.3 0 42 120 47 596.3 0 47 121 19 596.3 0 19 122 82 596.3 0 82 123 106 596.3 0 106 124 44 596.3 0 44 125 80 596.3 0 80 126 121 596.3 0 121 127 44 596.3 0 44 128 43 596.3 0 43 129 34 596.3 0 34 130 22 596.3 0 22 131 26 596.3 0 26 132 26 596.3 0 26 133 35 596.3 0 35 134 37 596.3 0 37 135 28 596.3 0 28 136 35 596.3 0 35 137 52 596.3 0 52 138 70 596.3 0 70 139 77 596.3 0 77 140 65 596.3 0 65 141 58 596.3 0 58 142 50 596.3 0 50 143 34 596.3 0 34 144 41 596.3 0 41 145 116 596.3 0 116 146 94 596.3 0 94 147 35 596.3 0 35 148 33 596.3 0 33 149 24 596.3 0 24 150 39 596.3 0 39 151 28 596.3 0 28 152 25 596.3 0 25 153 67 596.3 0 67 154 35 596.3 0 35 155 35 596.3 0 35 156 46 596.3 0 46 157 62 596.3 0 62 158 49 596.3 0 49 159 84 596.3 0 84 160 319 596.3 0 319 161 255 596.3 0 255 162 488 596.3 0 488 163 108 596.3 0 108 164 44 596.3 0 44 165 41 596.3 0 41 166 36 596.3 0 36 167 52 596.3 0 52 168 48 596.3 0 48 169 66 596.3 0 66 170 72 596.3 0 72 171 32 596.3 0 32 172 46 596.3 0 46 173 43 596.3 0 43 174 72 596.3 0 72 175 54 596.3 0 54 176 43 596.3 0 43 177 59 596.3 0 59 178 51 596.3 0 51 179 46 596.3 0 46 180 101 596.3 0 101 181 157 596.3 0 157 182 228 596.3 0 228 183 143 596.3 0 143 184 74 596.3 0 74 185 125 596.3 0 125 186 164 596.3 0 164 187 3246 596.3 0 3246 188 1139 596.3 0 1139 189 2236 596.3 0 2236 190 79 596.3 0 79 191 43 596.3 0 43 192 37 596.3 0 37 193 39 596.3 0 39 194 24 596.3 0 24 195 40 596.3 0 40 196 36 596.3 0 36 197 42 596.3 0 42 198 40 596.3 0 40 199 55 596.3 0 55 200 38 596.3 0 38 201 53 596.3 0 53 202 40 596.3 0 40 203 51 596.3 0 51 204 63 596.3 0 63 205 77 596.3 0 77 206 123 596.3 0 123 207 96 596.3 0 96 208 51 596.3 0 51 209 34 596.3 0 34 210 103 596.3 0 103 211 200 596.3 0 200 212 326 596.3 0 326 213 164 596.3 0 164 214 64 596.3 0 64 215 36 596.3 0 36 216 47 596.3 0 47 217 33 596.3 0 33 218 50 596.3 0 50 219 128 596.3 0 128 220 178 596.3 0 178 221 232 596.3 0 232 222 46 596.3 0 46 223 45 596.3 0 45 224 87 596.3 0 87 225 61 596.3 0 61 226 57 596.3 0 57 227 91 596.3 0 91 228 65 596.3 0 65 229 68 596.3 0 68 230 313 596.3 0 313 231 379 596.3 0 379 232 393 596.3 0 393 233 78 596.3 0 78 234 21 596.3 0 21 235 22 596.3 0 22 236 26 596.3 0 26 237 34 596.3 0 34 238 32 596.3 0 32 239 48 596.3 0 48 240 58 596.3 0 58 241 72 596.3 0 72 242 40 596.3 0 40 243 41 596.3 0 41 244 27 596.3 0 27 245 29 596.3 0 29 246 29 596.3 0 29 247 28 596.3 0 28 248 25 596.3 0 25 249 16 596.3 0 16 250 25 596.3 0 25 251 30 596.3 0 30 252 47 596.3 0 47 253 57 596.3 0 57 254 96 596.3 0 96 255 65 596.3 0 65 256 46 596.3 0 46 257 42 596.3 0 42 258 44 596.3 0 44 259 48 596.3 0 48 260 49 596.3 0 49 261 49 596.3 0 49 262 35 596.3 0 35 263 59 596.3 0 59 264 58 596.3 0 58 265 87 596.3 0 87 266 44 596.3 0 44 267 39 596.3 0 39 268 62 596.3 0 62 269 60 596.3 0 60 270 31 596.3 0 31 271 54 596.3 0 54 272 46 596.3 0 46 273 61 596.3 0 61 274 121 596.3 0 121 275 294 596.3 0 294 276 356 596.3 0 356 277 379 596.3 0 379 278 127 596.3 0 127 279 155 596.3 0 155 280 188 596.3 0 188 281 112 596.3 0 112 282 60 596.3 0 60 283 106 596.3 0 106 284 86 596.3 0 86 285 99 596.3 0 99 286 44 596.3 0 44 287 91 596.3 0 91 288 144 596.3 0 144 289 370 596.3 0 370 290 207 596.3 0 207 291 83 596.3 0 83 292 69 596.3 0 69 293 68 596.3 0 68 294 71 596.3 0 71 295 77 596.3 0 77 296 46 596.3 0 46 297 151 596.3 0 151 298 167 596.3 0 167 299 55 596.3 0 55 300 223 596.3 0 223 301 195 596.3 0 195 Finished in 0.76 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 21,412 Reads with adapters: 1,736 (8.1%) Reads that were too short: 31 (0.1%) Reads written (passing filters): 1,705 (8.0%) Total basepairs processed: 3,872,130 bp Total written (filtered): 464,375 bp (12.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1736 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 0 1 16 9 0.0 3 0 9 17 1640 0.0 3 1606 32 2 18 6 0.0 3 1 5 19 2 0.0 3 0 0 2 20 1 0.0 3 1 29 1 0.0 3 1 34 1 0.0 3 0 0 0 1 36 2 0.0 3 0 0 0 2 54 3 0.0 3 0 0 0 3 55 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 126 3 0.0 3 0 0 1 2 127 1 0.0 3 0 0 0 1 141 46 0.0 3 0 0 0 46 145 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.05 s (29 us/read; 2.05 M reads/minute). === Summary === Total reads processed: 1,705 Reads with adapters: 5 (0.3%) Reads written (passing filters): 1,705 (100.0%) Total basepairs processed: 464,375 bp Total written (filtered): 464,360 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 40.0% C: 60.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5 26.6 0 5