This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 62.69 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 83,625 (3.4%) Reads that were too short: 30,903 (1.3%) Reads written (passing filters): 52,722 (2.2%) Total basepairs processed: 735,190,694 bp Total written (filtered): 8,366,201 bp (1.1%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 83625 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 187 596.3 0 187 7 801 596.3 0 801 8 60 596.3 0 60 9 957 596.3 0 957 10 1094 596.3 0 1094 11 23 596.3 0 23 12 15 596.3 0 15 13 31 596.3 0 31 14 20 596.3 0 20 15 17 596.3 0 17 16 19 596.3 0 19 17 11 596.3 0 11 18 11 596.3 0 11 19 18 596.3 0 18 20 13 596.3 0 13 21 13 596.3 0 13 22 16 596.3 0 16 23 11 596.3 0 11 24 15 596.3 0 15 25 19 596.3 0 19 26 18 596.3 0 18 27 29 596.3 0 29 28 24 596.3 0 24 29 21 596.3 0 21 30 23 596.3 0 23 31 15 596.3 0 15 32 16 596.3 0 16 33 29 596.3 0 29 34 10 596.3 0 10 35 20 596.3 0 20 36 33 596.3 0 33 37 23 596.3 0 23 38 102 596.3 0 102 39 77 596.3 0 77 40 84 596.3 0 84 41 29 596.3 0 29 42 16 596.3 0 16 43 32 596.3 0 32 44 20 596.3 0 20 45 31 596.3 0 31 46 19 596.3 0 19 47 21 596.3 0 21 48 13 596.3 0 13 49 22 596.3 0 22 50 34 596.3 0 34 51 17 596.3 0 17 52 21 596.3 0 21 53 206 596.3 0 206 54 97 596.3 0 97 55 165 596.3 0 165 56 41 596.3 0 41 57 198 596.3 0 198 58 28 596.3 0 28 59 18 596.3 0 18 60 16 596.3 0 16 61 14 596.3 0 14 62 17 596.3 0 17 63 23 596.3 0 23 64 23 596.3 0 23 65 33 596.3 0 33 66 175 596.3 0 175 67 69 596.3 0 69 68 109 596.3 0 109 69 118 596.3 0 118 70 37 596.3 0 37 71 70 596.3 0 70 72 115 596.3 0 115 73 39 596.3 0 39 74 48 596.3 0 48 75 22 596.3 0 22 76 11 596.3 0 11 77 16 596.3 0 16 78 18 596.3 0 18 79 21 596.3 0 21 80 20 596.3 0 20 81 32 596.3 0 32 82 25 596.3 0 25 83 25 596.3 0 25 84 24 596.3 0 24 85 20 596.3 0 20 86 56 596.3 0 56 87 36 596.3 0 36 88 33 596.3 0 33 89 27 596.3 0 27 90 25 596.3 0 25 91 67 596.3 0 67 92 24 596.3 0 24 93 50 596.3 0 50 94 52 596.3 0 52 95 64 596.3 0 64 96 23 596.3 0 23 97 23 596.3 0 23 98 28 596.3 0 28 99 33 596.3 0 33 100 18 596.3 0 18 101 34 596.3 0 34 102 45 596.3 0 45 103 128 596.3 0 128 104 150 596.3 0 150 105 176 596.3 0 176 106 65 596.3 0 65 107 60 596.3 0 60 108 58 596.3 0 58 109 47 596.3 0 47 110 29 596.3 0 29 111 97 596.3 0 97 112 96 596.3 0 96 113 191 596.3 0 191 114 55 596.3 0 55 115 61 596.3 0 61 116 40 596.3 0 40 117 46 596.3 0 46 118 34 596.3 0 34 119 53 596.3 0 53 120 83 596.3 0 83 121 76 596.3 0 76 122 65 596.3 0 65 123 144 596.3 0 144 124 198 596.3 0 198 125 2018 596.3 0 2018 126 1093 596.3 0 1093 127 2591 596.3 0 2591 128 87 596.3 0 87 129 40 596.3 0 40 130 71 596.3 0 71 131 77 596.3 0 77 132 98 596.3 0 98 133 115 596.3 0 115 134 102 596.3 0 102 135 103 596.3 0 103 136 60 596.3 0 60 137 82 596.3 0 82 138 66 596.3 0 66 139 93 596.3 0 93 140 128 596.3 0 128 141 75 596.3 0 75 142 88 596.3 0 88 143 91 596.3 0 91 144 207 596.3 0 207 145 520 596.3 0 520 146 379 596.3 0 379 147 227 596.3 0 227 148 124 596.3 0 124 149 166 596.3 0 166 150 367 596.3 0 367 151 303 596.3 0 303 152 502 596.3 0 502 153 163 596.3 0 163 154 318 596.3 0 318 155 611 596.3 0 611 156 1512 596.3 0 1512 157 1633 596.3 0 1633 158 606 596.3 0 606 159 738 596.3 0 738 160 5400 596.3 0 5400 161 4632 596.3 0 4632 162 5940 596.3 0 5940 163 317 596.3 0 317 164 217 596.3 0 217 165 119 596.3 0 119 166 219 596.3 0 219 167 102 596.3 0 102 168 109 596.3 0 109 169 384 596.3 0 384 170 132 596.3 0 132 171 218 596.3 0 218 172 87 596.3 0 87 173 131 596.3 0 131 174 127 596.3 0 127 175 555 596.3 0 555 176 838 596.3 0 838 177 736 596.3 0 736 178 151 596.3 0 151 179 201 596.3 0 201 180 1287 596.3 0 1287 181 2001 596.3 0 2001 182 2354 596.3 0 2354 183 882 596.3 0 882 184 80 596.3 0 80 185 116 596.3 0 116 186 128 596.3 0 128 187 119 596.3 0 119 188 87 596.3 0 87 189 76 596.3 0 76 190 69 596.3 0 69 191 90 596.3 0 90 192 55 596.3 0 55 193 50 596.3 0 50 194 65 596.3 0 65 195 63 596.3 0 63 196 49 596.3 0 49 197 84 596.3 0 84 198 92 596.3 0 92 199 92 596.3 0 92 200 111 596.3 0 111 201 70 596.3 0 70 202 69 596.3 0 69 203 74 596.3 0 74 204 65 596.3 0 65 205 84 596.3 0 84 206 64 596.3 0 64 207 87 596.3 0 87 208 138 596.3 0 138 209 124 596.3 0 124 210 128 596.3 0 128 211 385 596.3 0 385 212 1373 596.3 0 1373 213 2030 596.3 0 2030 214 2479 596.3 0 2479 215 105 596.3 0 105 216 104 596.3 0 104 217 98 596.3 0 98 218 91 596.3 0 91 219 157 596.3 0 157 220 102 596.3 0 102 221 98 596.3 0 98 222 86 596.3 0 86 223 133 596.3 0 133 224 166 596.3 0 166 225 245 596.3 0 245 226 172 596.3 0 172 227 70 596.3 0 70 228 91 596.3 0 91 229 115 596.3 0 115 230 82 596.3 0 82 231 66 596.3 0 66 232 61 596.3 0 61 233 87 596.3 0 87 234 108 596.3 0 108 235 105 596.3 0 105 236 119 596.3 0 119 237 99 596.3 0 99 238 117 596.3 0 117 239 189 596.3 0 189 240 151 596.3 0 151 241 161 596.3 0 161 242 112 596.3 0 112 243 161 596.3 0 161 244 113 596.3 0 113 245 188 596.3 0 188 246 220 596.3 0 220 247 276 596.3 0 276 248 221 596.3 0 221 249 157 596.3 0 157 250 98 596.3 0 98 251 98 596.3 0 98 252 130 596.3 0 130 253 129 596.3 0 129 254 174 596.3 0 174 255 390 596.3 0 390 256 1429 596.3 0 1429 257 908 596.3 0 908 258 1401 596.3 0 1401 259 281 596.3 0 281 260 230 596.3 0 230 261 209 596.3 0 209 262 178 596.3 0 178 263 153 596.3 0 153 264 152 596.3 0 152 265 104 596.3 0 104 266 437 596.3 0 437 267 552 596.3 0 552 268 1023 596.3 0 1023 269 297 596.3 0 297 270 172 596.3 0 172 271 244 596.3 0 244 272 621 596.3 0 621 273 1103 596.3 0 1103 274 1323 596.3 0 1323 275 955 596.3 0 955 276 254 596.3 0 254 277 230 596.3 0 230 278 309 596.3 0 309 279 239 596.3 0 239 280 426 596.3 0 426 281 343 596.3 0 343 282 304 596.3 0 304 283 215 596.3 0 215 284 277 596.3 0 277 285 169 596.3 0 169 286 206 596.3 0 206 287 466 596.3 0 466 288 372 596.3 0 372 289 487 596.3 0 487 290 120 596.3 0 120 291 117 596.3 0 117 292 156 596.3 0 156 293 316 596.3 0 316 294 251 596.3 0 251 295 218 596.3 0 218 296 122 596.3 0 122 297 136 596.3 0 136 298 92 596.3 0 92 299 92 596.3 0 92 300 153 596.3 0 153 301 316 596.3 0 316 Finished in 1.87 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 52,722 Reads with adapters: 1,107 (2.1%) Reads that were too short: 39 (0.1%) Reads written (passing filters): 1,068 (2.0%) Total basepairs processed: 8,366,201 bp Total written (filtered): 269,316 bp (3.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1107 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 126 0.0 3 0 0 0 0 126 19 13 0.0 3 0 10 1 1 1 20 787 0.0 4 741 39 5 2 21 4 0.0 4 0 2 0 0 2 22 3 0.0 4 0 3 23 101 0.0 4 95 4 0 2 29 2 0.0 4 0 0 0 0 2 34 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 1 57 1 0.0 4 0 0 0 0 1 60 10 0.0 4 0 0 0 0 10 61 5 0.0 4 0 0 0 0 5 62 3 0.0 4 0 0 0 0 3 63 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 109 3 0.0 4 0 0 0 0 3 110 2 0.0 4 0 0 0 0 2 117 1 0.0 4 0 0 0 0 1 126 16 0.0 4 0 0 0 0 16 127 10 0.0 4 0 0 0 0 10 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 1 222 1 0.0 4 0 0 0 1 228 1 0.0 4 0 0 0 0 1 252 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (28 us/read; 2.14 M reads/minute). === Summary === Total reads processed: 1,068 Reads with adapters: 107 (10.0%) Reads written (passing filters): 1,068 (100.0%) Total basepairs processed: 269,316 bp Total written (filtered): 268,195 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 107 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.6% C: 9.3% G: 85.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 16.7 0 3 4 2 4.2 0 2 9 5 0.0 0 0 5 10 23 0.0 1 3 20 11 61 0.0 1 2 59 12 11 0.0 1 1 10 13 2 0.0 1 0 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.