This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 62.51 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 55,220 (2.3%) Reads that were too short: 17,975 (0.7%) Reads written (passing filters): 37,245 (1.5%) Total basepairs processed: 735,190,694 bp Total written (filtered): 6,008,192 bp (0.8%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 55220 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 178 596.3 0 178 7 988 596.3 0 988 8 19 596.3 0 19 9 868 596.3 0 868 10 126 596.3 0 126 11 13 596.3 0 13 12 16 596.3 0 16 13 9 596.3 0 9 14 19 596.3 0 19 15 10 596.3 0 10 16 12 596.3 0 12 17 13 596.3 0 13 18 7 596.3 0 7 19 11 596.3 0 11 20 15 596.3 0 15 21 18 596.3 0 18 22 12 596.3 0 12 23 9 596.3 0 9 24 5 596.3 0 5 25 13 596.3 0 13 26 9 596.3 0 9 27 12 596.3 0 12 28 16 596.3 0 16 29 10 596.3 0 10 30 13 596.3 0 13 31 12 596.3 0 12 32 14 596.3 0 14 33 18 596.3 0 18 34 25 596.3 0 25 35 24 596.3 0 24 36 19 596.3 0 19 37 16 596.3 0 16 38 76 596.3 0 76 39 56 596.3 0 56 40 92 596.3 0 92 41 12 596.3 0 12 42 11 596.3 0 11 43 16 596.3 0 16 44 11 596.3 0 11 45 16 596.3 0 16 46 18 596.3 0 18 47 9 596.3 0 9 48 8 596.3 0 8 49 22 596.3 0 22 50 14 596.3 0 14 51 15 596.3 0 15 52 24 596.3 0 24 53 138 596.3 0 138 54 79 596.3 0 79 55 126 596.3 0 126 56 29 596.3 0 29 57 203 596.3 0 203 58 18 596.3 0 18 59 16 596.3 0 16 60 26 596.3 0 26 61 21 596.3 0 21 62 10 596.3 0 10 63 14 596.3 0 14 64 16 596.3 0 16 65 24 596.3 0 24 66 63 596.3 0 63 67 17 596.3 0 17 68 39 596.3 0 39 69 135 596.3 0 135 70 25 596.3 0 25 71 50 596.3 0 50 72 27 596.3 0 27 73 19 596.3 0 19 74 14 596.3 0 14 75 18 596.3 0 18 76 13 596.3 0 13 77 16 596.3 0 16 78 8 596.3 0 8 79 7 596.3 0 7 80 14 596.3 0 14 81 17 596.3 0 17 82 18 596.3 0 18 83 26 596.3 0 26 84 12 596.3 0 12 85 17 596.3 0 17 86 18 596.3 0 18 87 26 596.3 0 26 88 23 596.3 0 23 89 31 596.3 0 31 90 28 596.3 0 28 91 57 596.3 0 57 92 27 596.3 0 27 93 40 596.3 0 40 94 39 596.3 0 39 95 34 596.3 0 34 96 24 596.3 0 24 97 18 596.3 0 18 98 25 596.3 0 25 99 13 596.3 0 13 100 16 596.3 0 16 101 27 596.3 0 27 102 26 596.3 0 26 103 51 596.3 0 51 104 70 596.3 0 70 105 65 596.3 0 65 106 36 596.3 0 36 107 35 596.3 0 35 108 32 596.3 0 32 109 37 596.3 0 37 110 38 596.3 0 38 111 45 596.3 0 45 112 37 596.3 0 37 113 83 596.3 0 83 114 27 596.3 0 27 115 48 596.3 0 48 116 26 596.3 0 26 117 51 596.3 0 51 118 30 596.3 0 30 119 45 596.3 0 45 120 54 596.3 0 54 121 58 596.3 0 58 122 53 596.3 0 53 123 96 596.3 0 96 124 174 596.3 0 174 125 2092 596.3 0 2092 126 1104 596.3 0 1104 127 2772 596.3 0 2772 128 72 596.3 0 72 129 37 596.3 0 37 130 35 596.3 0 35 131 42 596.3 0 42 132 67 596.3 0 67 133 70 596.3 0 70 134 50 596.3 0 50 135 44 596.3 0 44 136 19 596.3 0 19 137 33 596.3 0 33 138 26 596.3 0 26 139 40 596.3 0 40 140 51 596.3 0 51 141 29 596.3 0 29 142 54 596.3 0 54 143 80 596.3 0 80 144 105 596.3 0 105 145 283 596.3 0 283 146 189 596.3 0 189 147 158 596.3 0 158 148 77 596.3 0 77 149 95 596.3 0 95 150 303 596.3 0 303 151 188 596.3 0 188 152 293 596.3 0 293 153 83 596.3 0 83 154 180 596.3 0 180 155 239 596.3 0 239 156 571 596.3 0 571 157 564 596.3 0 564 158 382 596.3 0 382 159 471 596.3 0 471 160 4895 596.3 0 4895 161 4392 596.3 0 4392 162 5284 596.3 0 5284 163 121 596.3 0 121 164 94 596.3 0 94 165 58 596.3 0 58 166 79 596.3 0 79 167 64 596.3 0 64 168 67 596.3 0 67 169 462 596.3 0 462 170 67 596.3 0 67 171 236 596.3 0 236 172 41 596.3 0 41 173 68 596.3 0 68 174 57 596.3 0 57 175 252 596.3 0 252 176 276 596.3 0 276 177 268 596.3 0 268 178 58 596.3 0 58 179 85 596.3 0 85 180 416 596.3 0 416 181 812 596.3 0 812 182 942 596.3 0 942 183 454 596.3 0 454 184 56 596.3 0 56 185 64 596.3 0 64 186 78 596.3 0 78 187 75 596.3 0 75 188 59 596.3 0 59 189 40 596.3 0 40 190 41 596.3 0 41 191 52 596.3 0 52 192 37 596.3 0 37 193 31 596.3 0 31 194 39 596.3 0 39 195 44 596.3 0 44 196 40 596.3 0 40 197 48 596.3 0 48 198 46 596.3 0 46 199 55 596.3 0 55 200 53 596.3 0 53 201 54 596.3 0 54 202 38 596.3 0 38 203 55 596.3 0 55 204 42 596.3 0 42 205 45 596.3 0 45 206 32 596.3 0 32 207 57 596.3 0 57 208 76 596.3 0 76 209 58 596.3 0 58 210 99 596.3 0 99 211 306 596.3 0 306 212 1312 596.3 0 1312 213 1603 596.3 0 1603 214 2831 596.3 0 2831 215 73 596.3 0 73 216 44 596.3 0 44 217 52 596.3 0 52 218 62 596.3 0 62 219 87 596.3 0 87 220 52 596.3 0 52 221 54 596.3 0 54 222 47 596.3 0 47 223 42 596.3 0 42 224 67 596.3 0 67 225 105 596.3 0 105 226 104 596.3 0 104 227 51 596.3 0 51 228 53 596.3 0 53 229 64 596.3 0 64 230 48 596.3 0 48 231 37 596.3 0 37 232 31 596.3 0 31 233 39 596.3 0 39 234 61 596.3 0 61 235 60 596.3 0 60 236 65 596.3 0 65 237 63 596.3 0 63 238 106 596.3 0 106 239 143 596.3 0 143 240 102 596.3 0 102 241 103 596.3 0 103 242 50 596.3 0 50 243 112 596.3 0 112 244 66 596.3 0 66 245 74 596.3 0 74 246 93 596.3 0 93 247 112 596.3 0 112 248 113 596.3 0 113 249 67 596.3 0 67 250 49 596.3 0 49 251 48 596.3 0 48 252 90 596.3 0 90 253 60 596.3 0 60 254 81 596.3 0 81 255 157 596.3 0 157 256 494 596.3 0 494 257 261 596.3 0 261 258 550 596.3 0 550 259 156 596.3 0 156 260 137 596.3 0 137 261 86 596.3 0 86 262 77 596.3 0 77 263 78 596.3 0 78 264 72 596.3 0 72 265 69 596.3 0 69 266 177 596.3 0 177 267 236 596.3 0 236 268 357 596.3 0 357 269 139 596.3 0 139 270 97 596.3 0 97 271 115 596.3 0 115 272 283 596.3 0 283 273 532 596.3 0 532 274 650 596.3 0 650 275 605 596.3 0 605 276 142 596.3 0 142 277 194 596.3 0 194 278 293 596.3 0 293 279 136 596.3 0 136 280 170 596.3 0 170 281 129 596.3 0 129 282 121 596.3 0 121 283 84 596.3 0 84 284 118 596.3 0 118 285 83 596.3 0 83 286 104 596.3 0 104 287 237 596.3 0 237 288 159 596.3 0 159 289 192 596.3 0 192 290 66 596.3 0 66 291 55 596.3 0 55 292 92 596.3 0 92 293 159 596.3 0 159 294 130 596.3 0 130 295 102 596.3 0 102 296 78 596.3 0 78 297 88 596.3 0 88 298 65 596.3 0 65 299 66 596.3 0 66 300 71 596.3 0 71 301 129 596.3 0 129 Finished in 1.20 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 37,245 Reads with adapters: 920 (2.5%) Reads that were too short: 36 (0.1%) Reads written (passing filters): 884 (2.4%) Total basepairs processed: 6,008,192 bp Total written (filtered): 241,789 bp (4.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 920 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 4 0.0 3 0 4 17 857 0.0 3 837 19 1 18 3 0.0 3 0 2 1 19 10 0.0 3 7 1 0 2 22 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 47 4 0.0 3 0 0 0 4 56 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 71 5 0.0 3 0 0 0 5 74 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 0 1 113 3 0.0 3 0 0 0 3 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 163 6 0.0 3 0 0 0 6 164 3 0.0 3 0 0 0 3 165 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.02 s (23 us/read; 2.65 M reads/minute). === Summary === Total reads processed: 884 Reads with adapters: 49 (5.5%) Reads written (passing filters): 884 (100.0%) Total basepairs processed: 241,789 bp Total written (filtered): 241,642 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 49 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 75.5% C: 4.1% G: 20.4% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 49 13.8 0 49