This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.20 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 50,370 (2.0%) Reads that were too short: 10,770 (0.4%) Reads written (passing filters): 39,600 (1.6%) Total basepairs processed: 740,966,583 bp Total written (filtered): 10,203,813 bp (1.4%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 50370 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 244 601.0 0 244 7 329 601.0 0 329 8 14790 601.0 0 14790 9 11388 601.0 0 11388 10 53 601.0 0 53 11 2 601.0 0 2 13 3 601.0 0 3 14 3 601.0 0 3 15 2 601.0 0 2 16 1 601.0 0 1 18 3 601.0 0 3 19 8 601.0 0 8 20 3 601.0 0 3 21 13 601.0 0 13 22 6 601.0 0 6 23 13 601.0 0 13 24 17 601.0 0 17 25 18 601.0 0 18 26 5 601.0 0 5 27 5 601.0 0 5 28 4 601.0 0 4 29 14 601.0 0 14 30 12 601.0 0 12 31 7 601.0 0 7 32 18 601.0 0 18 33 42 601.0 0 42 34 23 601.0 0 23 35 109 601.0 0 109 36 22 601.0 0 22 37 69 601.0 0 69 38 43 601.0 0 43 39 21 601.0 0 21 40 15 601.0 0 15 41 9 601.0 0 9 42 3 601.0 0 3 43 9 601.0 0 9 44 8 601.0 0 8 45 10 601.0 0 10 46 8 601.0 0 8 47 9 601.0 0 9 48 5 601.0 0 5 49 5 601.0 0 5 50 10 601.0 0 10 51 14 601.0 0 14 52 9 601.0 0 9 53 14 601.0 0 14 54 15 601.0 0 15 55 10 601.0 0 10 56 6 601.0 0 6 57 16 601.0 0 16 58 69 601.0 0 69 59 90 601.0 0 90 60 154 601.0 0 154 61 9 601.0 0 9 62 14 601.0 0 14 63 19 601.0 0 19 64 8 601.0 0 8 65 6 601.0 0 6 66 17 601.0 0 17 67 11 601.0 0 11 68 22 601.0 0 22 69 16 601.0 0 16 70 17 601.0 0 17 71 35 601.0 0 35 72 10 601.0 0 10 73 95 601.0 0 95 74 19 601.0 0 19 75 30 601.0 0 30 76 231 601.0 0 231 77 222 601.0 0 222 78 573 601.0 0 573 79 16 601.0 0 16 80 268 601.0 0 268 81 220 601.0 0 220 82 365 601.0 0 365 83 19 601.0 0 19 84 8 601.0 0 8 85 19 601.0 0 19 86 10 601.0 0 10 87 14 601.0 0 14 88 12 601.0 0 12 89 14 601.0 0 14 90 18 601.0 0 18 91 12 601.0 0 12 92 4 601.0 0 4 93 12 601.0 0 12 94 20 601.0 0 20 95 18 601.0 0 18 96 49 601.0 0 49 97 15 601.0 0 15 98 14 601.0 0 14 99 40 601.0 0 40 100 390 601.0 0 390 101 1351 601.0 0 1351 102 2156 601.0 0 2156 103 9 601.0 0 9 104 19 601.0 0 19 105 11 601.0 0 11 106 8 601.0 0 8 107 13 601.0 0 13 108 11 601.0 0 11 109 48 601.0 0 48 110 266 601.0 0 266 111 325 601.0 0 325 112 306 601.0 0 306 113 24 601.0 0 24 114 10 601.0 0 10 115 14 601.0 0 14 116 7 601.0 0 7 117 38 601.0 0 38 118 12 601.0 0 12 119 9 601.0 0 9 120 17 601.0 0 17 121 5 601.0 0 5 122 10 601.0 0 10 123 12 601.0 0 12 124 14 601.0 0 14 125 9 601.0 0 9 126 8 601.0 0 8 127 99 601.0 0 99 128 21 601.0 0 21 129 17 601.0 0 17 130 89 601.0 0 89 131 27 601.0 0 27 132 29 601.0 0 29 133 22 601.0 0 22 134 9 601.0 0 9 135 13 601.0 0 13 136 49 601.0 0 49 137 6 601.0 0 6 138 23 601.0 0 23 139 11 601.0 0 11 140 14 601.0 0 14 141 8 601.0 0 8 142 36 601.0 0 36 143 26 601.0 0 26 144 16 601.0 0 16 145 21 601.0 0 21 146 25 601.0 0 25 147 80 601.0 0 80 148 57 601.0 0 57 149 186 601.0 0 186 150 110 601.0 0 110 151 95 601.0 0 95 152 410 601.0 0 410 153 22 601.0 0 22 154 10 601.0 0 10 155 28 601.0 0 28 156 17 601.0 0 17 157 36 601.0 0 36 158 52 601.0 0 52 159 23 601.0 0 23 160 15 601.0 0 15 161 16 601.0 0 16 162 49 601.0 0 49 163 11 601.0 0 11 164 13 601.0 0 13 165 14 601.0 0 14 166 14 601.0 0 14 167 13 601.0 0 13 168 56 601.0 0 56 169 73 601.0 0 73 170 201 601.0 0 201 171 11 601.0 0 11 172 5 601.0 0 5 173 6 601.0 0 6 174 6 601.0 0 6 175 14 601.0 0 14 176 18 601.0 0 18 177 48 601.0 0 48 178 37 601.0 0 37 179 32 601.0 0 32 180 22 601.0 0 22 181 25 601.0 0 25 182 105 601.0 0 105 183 183 601.0 0 183 184 59 601.0 0 59 185 29 601.0 0 29 186 34 601.0 0 34 187 26 601.0 0 26 188 54 601.0 0 54 189 34 601.0 0 34 190 26 601.0 0 26 191 13 601.0 0 13 192 16 601.0 0 16 193 20 601.0 0 20 194 33 601.0 0 33 195 39 601.0 0 39 196 45 601.0 0 45 197 182 601.0 0 182 198 140 601.0 0 140 199 278 601.0 0 278 200 278 601.0 0 278 201 219 601.0 0 219 202 998 601.0 0 998 203 413 601.0 0 413 204 273 601.0 0 273 205 67 601.0 0 67 206 55 601.0 0 55 207 33 601.0 0 33 208 72 601.0 0 72 209 29 601.0 0 29 210 28 601.0 0 28 211 31 601.0 0 31 212 25 601.0 0 25 213 33 601.0 0 33 214 33 601.0 0 33 215 36 601.0 0 36 216 33 601.0 0 33 217 149 601.0 0 149 218 161 601.0 0 161 219 213 601.0 0 213 220 54 601.0 0 54 221 78 601.0 0 78 222 1198 601.0 0 1198 223 483 601.0 0 483 224 614 601.0 0 614 225 61 601.0 0 61 226 38 601.0 0 38 227 67 601.0 0 67 228 68 601.0 0 68 229 58 601.0 0 58 230 27 601.0 0 27 231 19 601.0 0 19 232 30 601.0 0 30 233 40 601.0 0 40 234 26 601.0 0 26 235 19 601.0 0 19 236 22 601.0 0 22 237 40 601.0 0 40 238 23 601.0 0 23 239 55 601.0 0 55 240 57 601.0 0 57 241 137 601.0 0 137 242 174 601.0 0 174 243 86 601.0 0 86 244 30 601.0 0 30 245 19 601.0 0 19 246 18 601.0 0 18 247 20 601.0 0 20 248 39 601.0 0 39 249 25 601.0 0 25 250 29 601.0 0 29 251 43 601.0 0 43 252 29 601.0 0 29 253 51 601.0 0 51 254 31 601.0 0 31 255 108 601.0 0 108 256 258 601.0 0 258 257 193 601.0 0 193 258 184 601.0 0 184 259 133 601.0 0 133 260 74 601.0 0 74 261 42 601.0 0 42 262 99 601.0 0 99 263 82 601.0 0 82 264 76 601.0 0 76 265 35 601.0 0 35 266 23 601.0 0 23 267 28 601.0 0 28 268 36 601.0 0 36 269 39 601.0 0 39 270 34 601.0 0 34 271 50 601.0 0 50 272 49 601.0 0 49 273 32 601.0 0 32 274 31 601.0 0 31 275 40 601.0 0 40 276 83 601.0 0 83 277 68 601.0 0 68 278 142 601.0 0 142 279 53 601.0 0 53 280 52 601.0 0 52 281 39 601.0 0 39 282 54 601.0 0 54 283 46 601.0 0 46 284 65 601.0 0 65 285 85 601.0 0 85 286 33 601.0 0 33 287 82 601.0 0 82 288 49 601.0 0 49 289 95 601.0 0 95 290 90 601.0 0 90 291 89 601.0 0 89 292 132 601.0 0 132 293 161 601.0 0 161 294 123 601.0 0 123 295 566 601.0 0 566 296 111 601.0 0 111 297 113 601.0 0 113 298 102 601.0 0 102 299 77 601.0 0 77 300 70 601.0 0 70 301 54 601.0 0 54 Finished in 2.07 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 39,600 Reads with adapters: 11,779 (29.7%) Reads that were too short: 29 (0.1%) Reads written (passing filters): 11,750 (29.7%) Total basepairs processed: 10,203,813 bp Total written (filtered): 3,194,712 bp (31.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11779 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 8 0.0 3 0 0 2 5 1 19 66 0.0 3 0 50 9 4 3 20 11572 0.0 4 10760 631 136 29 16 21 20 0.0 4 2 14 3 1 22 6 0.0 4 0 0 3 2 1 23 59 0.0 4 8 4 3 31 13 24 1 0.0 4 0 0 0 0 1 26 2 0.0 4 0 0 0 2 30 1 0.0 4 1 38 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 69 2 0.0 4 0 0 0 0 2 77 1 0.0 4 0 0 0 0 1 78 2 0.0 4 0 0 0 0 2 79 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 1 100 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 1 183 3 0.0 4 0 0 0 0 3 186 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 1 0.0 4 0 0 0 0 1 230 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 0 2 258 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 272 1 0.0 4 0 0 0 0 1 278 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.43 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 11,750 Reads with adapters: 2,580 (22.0%) Reads written (passing filters): 11,750 (100.0%) Total basepairs processed: 3,194,712 bp Total written (filtered): 3,168,843 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2580 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.9% C: 8.3% G: 86.9% T: 2.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 183.6 0 4 4 1 45.9 0 1 8 4 0.2 0 4 9 163 0.0 0 11 152 10 2150 0.0 1 145 2005 11 247 0.0 1 7 240 12 6 0.0 1 0 6 13 5 0.0 1 0 5 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.