This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.77 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 236,130 (9.6%) Reads that were too short: 4,557 (0.2%) Reads written (passing filters): 231,573 (9.4%) Total basepairs processed: 740,966,583 bp Total written (filtered): 45,900,105 bp (6.2%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 236130 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 780 601.0 0 780 7 36703 601.0 0 36703 8 32124 601.0 0 32124 9 25 601.0 0 25 10 13 601.0 0 13 11 78 601.0 0 78 12 102 601.0 0 102 13 72 601.0 0 72 14 6 601.0 0 6 15 6 601.0 0 6 16 3 601.0 0 3 17 12 601.0 0 12 18 2 601.0 0 2 19 7 601.0 0 7 20 13 601.0 0 13 21 11 601.0 0 11 22 12 601.0 0 12 23 7 601.0 0 7 24 11 601.0 0 11 25 19 601.0 0 19 26 37 601.0 0 37 27 54 601.0 0 54 28 15 601.0 0 15 29 3 601.0 0 3 30 6 601.0 0 6 31 15 601.0 0 15 32 19 601.0 0 19 33 19 601.0 0 19 34 17 601.0 0 17 35 15 601.0 0 15 36 14 601.0 0 14 37 12 601.0 0 12 38 70 601.0 0 70 39 34 601.0 0 34 40 16 601.0 0 16 41 6 601.0 0 6 42 16 601.0 0 16 43 19 601.0 0 19 44 22 601.0 0 22 45 14 601.0 0 14 46 6 601.0 0 6 47 10 601.0 0 10 48 10 601.0 0 10 49 6 601.0 0 6 50 11 601.0 0 11 51 16 601.0 0 16 52 14 601.0 0 14 53 7 601.0 0 7 54 16 601.0 0 16 55 14 601.0 0 14 56 13 601.0 0 13 57 7 601.0 0 7 58 7 601.0 0 7 59 16 601.0 0 16 60 27 601.0 0 27 61 8 601.0 0 8 62 7 601.0 0 7 63 6 601.0 0 6 64 11 601.0 0 11 65 9 601.0 0 9 66 5 601.0 0 5 67 10 601.0 0 10 68 11 601.0 0 11 69 23 601.0 0 23 70 11 601.0 0 11 71 17 601.0 0 17 72 14 601.0 0 14 73 19 601.0 0 19 74 97 601.0 0 97 75 16 601.0 0 16 76 18 601.0 0 18 77 4 601.0 0 4 78 7 601.0 0 7 79 19 601.0 0 19 80 75 601.0 0 75 81 17 601.0 0 17 82 64 601.0 0 64 83 8 601.0 0 8 84 9 601.0 0 9 85 19 601.0 0 19 86 12 601.0 0 12 87 8 601.0 0 8 88 10 601.0 0 10 89 19 601.0 0 19 90 13 601.0 0 13 91 20 601.0 0 20 92 23 601.0 0 23 93 10 601.0 0 10 94 21 601.0 0 21 95 7 601.0 0 7 96 26 601.0 0 26 97 37 601.0 0 37 98 70 601.0 0 70 99 29 601.0 0 29 100 19 601.0 0 19 101 15 601.0 0 15 102 68 601.0 0 68 103 72 601.0 0 72 104 114 601.0 0 114 105 110 601.0 0 110 106 59 601.0 0 59 107 75 601.0 0 75 108 65 601.0 0 65 109 36 601.0 0 36 110 27 601.0 0 27 111 36 601.0 0 36 112 81 601.0 0 81 113 51 601.0 0 51 114 51 601.0 0 51 115 288 601.0 0 288 116 25 601.0 0 25 117 20 601.0 0 20 118 14 601.0 0 14 119 26 601.0 0 26 120 38 601.0 0 38 121 44 601.0 0 44 122 158 601.0 0 158 123 205 601.0 0 205 124 437 601.0 0 437 125 408 601.0 0 408 126 105 601.0 0 105 127 154 601.0 0 154 128 93 601.0 0 93 129 100 601.0 0 100 130 287 601.0 0 287 131 110 601.0 0 110 132 153 601.0 0 153 133 132 601.0 0 132 134 145 601.0 0 145 135 363 601.0 0 363 136 301 601.0 0 301 137 502 601.0 0 502 138 883 601.0 0 883 139 1091 601.0 0 1091 140 1767 601.0 0 1767 141 3210 601.0 0 3210 142 33741 601.0 0 33741 143 19921 601.0 0 19921 144 77353 601.0 0 77353 145 321 601.0 0 321 146 42 601.0 0 42 147 64 601.0 0 64 148 24 601.0 0 24 149 62 601.0 0 62 150 28 601.0 0 28 151 36 601.0 0 36 152 36 601.0 0 36 153 34 601.0 0 34 154 31 601.0 0 31 155 50 601.0 0 50 156 71 601.0 0 71 157 93 601.0 0 93 158 93 601.0 0 93 159 125 601.0 0 125 160 241 601.0 0 241 161 511 601.0 0 511 162 3954 601.0 0 3954 163 2203 601.0 0 2203 164 8177 601.0 0 8177 165 32 601.0 0 32 166 17 601.0 0 17 167 27 601.0 0 27 168 38 601.0 0 38 169 59 601.0 0 59 170 11 601.0 0 11 171 8 601.0 0 8 172 15 601.0 0 15 173 11 601.0 0 11 174 4 601.0 0 4 175 16 601.0 0 16 176 19 601.0 0 19 177 57 601.0 0 57 178 44 601.0 0 44 179 25 601.0 0 25 180 14 601.0 0 14 181 15 601.0 0 15 182 14 601.0 0 14 183 21 601.0 0 21 184 18 601.0 0 18 185 29 601.0 0 29 186 9 601.0 0 9 187 29 601.0 0 29 188 47 601.0 0 47 189 85 601.0 0 85 190 190 601.0 0 190 191 10 601.0 0 10 192 9 601.0 0 9 193 18 601.0 0 18 194 5 601.0 0 5 195 15 601.0 0 15 196 21 601.0 0 21 197 13 601.0 0 13 198 12 601.0 0 12 199 20 601.0 0 20 200 11 601.0 0 11 201 18 601.0 0 18 202 14 601.0 0 14 203 15 601.0 0 15 204 10 601.0 0 10 205 12 601.0 0 12 206 16 601.0 0 16 207 36 601.0 0 36 208 22 601.0 0 22 209 30 601.0 0 30 210 71 601.0 0 71 211 108 601.0 0 108 212 148 601.0 0 148 213 38 601.0 0 38 214 77 601.0 0 77 215 107 601.0 0 107 216 280 601.0 0 280 217 127 601.0 0 127 218 26 601.0 0 26 219 14 601.0 0 14 220 12 601.0 0 12 221 31 601.0 0 31 222 29 601.0 0 29 223 50 601.0 0 50 224 46 601.0 0 46 225 48 601.0 0 48 226 53 601.0 0 53 227 69 601.0 0 69 228 20 601.0 0 20 229 19 601.0 0 19 230 23 601.0 0 23 231 16 601.0 0 16 232 91 601.0 0 91 233 65 601.0 0 65 234 48 601.0 0 48 235 11 601.0 0 11 236 19 601.0 0 19 237 41 601.0 0 41 238 45 601.0 0 45 239 37 601.0 0 37 240 42 601.0 0 42 241 32 601.0 0 32 242 96 601.0 0 96 243 23 601.0 0 23 244 18 601.0 0 18 245 28 601.0 0 28 246 19 601.0 0 19 247 26 601.0 0 26 248 14 601.0 0 14 249 13 601.0 0 13 250 24 601.0 0 24 251 16 601.0 0 16 252 21 601.0 0 21 253 16 601.0 0 16 254 14 601.0 0 14 255 42 601.0 0 42 256 53 601.0 0 53 257 93 601.0 0 93 258 53 601.0 0 53 259 52 601.0 0 52 260 51 601.0 0 51 261 83 601.0 0 83 262 131 601.0 0 131 263 85 601.0 0 85 264 56 601.0 0 56 265 27 601.0 0 27 266 23 601.0 0 23 267 18 601.0 0 18 268 28 601.0 0 28 269 43 601.0 0 43 270 40 601.0 0 40 271 32 601.0 0 32 272 19 601.0 0 19 273 13 601.0 0 13 274 42 601.0 0 42 275 29 601.0 0 29 276 60 601.0 0 60 277 37 601.0 0 37 278 77 601.0 0 77 279 180 601.0 0 180 280 93 601.0 0 93 281 73 601.0 0 73 282 46 601.0 0 46 283 61 601.0 0 61 284 48 601.0 0 48 285 46 601.0 0 46 286 31 601.0 0 31 287 38 601.0 0 38 288 29 601.0 0 29 289 28 601.0 0 28 290 27 601.0 0 27 291 41 601.0 0 41 292 19 601.0 0 19 293 23 601.0 0 23 294 29 601.0 0 29 295 48 601.0 0 48 296 55 601.0 0 55 297 41 601.0 0 41 298 25 601.0 0 25 299 24 601.0 0 24 300 27 601.0 0 27 301 12 601.0 0 12 Finished in 9.38 s (41 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 231,573 Reads with adapters: 33,160 (14.3%) Reads that were too short: 41 (0.0%) Reads written (passing filters): 33,119 (14.3%) Total basepairs processed: 45,900,105 bp Total written (filtered): 9,015,067 bp (19.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 33160 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 0 2 17 2 0.0 3 0 0 0 2 18 10 0.0 3 0 0 4 1 5 19 266 0.0 3 0 176 42 31 17 20 32343 0.0 4 29839 1988 375 108 33 21 85 0.0 4 14 64 7 22 12 0.0 4 3 0 6 2 1 23 148 0.0 4 0 1 2 101 44 26 1 0.0 4 0 0 0 0 1 37 7 0.0 4 0 0 0 0 7 38 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 1 81 2 0.0 4 0 0 0 0 2 82 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 233 0.0 4 0 0 0 0 233 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 1 143 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 202 2 0.0 4 0 0 0 0 2 208 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 5 0.0 4 0 0 0 0 5 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 238 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 250 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 259 2 0.0 4 0 0 0 0 2 260 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 2 0.0 4 0 0 0 0 2 289 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.23 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 33,119 Reads with adapters: 7,098 (21.4%) Reads written (passing filters): 33,119 (100.0%) Total basepairs processed: 9,015,067 bp Total written (filtered): 8,928,309 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7098 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.1% C: 10.8% G: 84.4% T: 2.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 61 517.5 0 61 4 43 129.4 0 43 5 1 32.3 0 1 6 1 8.1 0 1 7 1 2.0 0 1 8 2 0.5 0 2 9 155 0.1 0 7 148 10 173 0.0 1 5 168 11 6575 0.0 1 210 6365 12 26 0.0 1 3 23 14 9 0.0 1 0 9 15 1 0.0 1 0 1 111 1 0.0 1 1 151 4 0.0 1 2 2 178 1 0.0 1 0 1 209 19 0.0 1 16 3 224 24 0.0 1 23 1 226 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.