This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.21 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 285,391 (11.6%) Reads that were too short: 3,021 (0.1%) Reads written (passing filters): 282,370 (11.5%) Total basepairs processed: 740,966,583 bp Total written (filtered): 54,380,685 bp (7.3%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 285391 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 636 601.0 0 636 7 32545 601.0 0 32545 8 40791 601.0 0 40791 9 31 601.0 0 31 10 15 601.0 0 15 11 38 601.0 0 38 12 89 601.0 0 89 13 20 601.0 0 20 14 6 601.0 0 6 15 6 601.0 0 6 16 3 601.0 0 3 17 9 601.0 0 9 18 6 601.0 0 6 19 10 601.0 0 10 20 12 601.0 0 12 21 17 601.0 0 17 22 5 601.0 0 5 23 6 601.0 0 6 24 11 601.0 0 11 25 16 601.0 0 16 26 9 601.0 0 9 27 25 601.0 0 25 28 11 601.0 0 11 29 3 601.0 0 3 30 10 601.0 0 10 31 12 601.0 0 12 32 10 601.0 0 10 33 14 601.0 0 14 34 12 601.0 0 12 35 10 601.0 0 10 36 5 601.0 0 5 37 2 601.0 0 2 38 7 601.0 0 7 39 6 601.0 0 6 40 2 601.0 0 2 41 12 601.0 0 12 42 8 601.0 0 8 43 24 601.0 0 24 44 23 601.0 0 23 45 15 601.0 0 15 46 8 601.0 0 8 47 9 601.0 0 9 48 6 601.0 0 6 49 5 601.0 0 5 50 12 601.0 0 12 51 12 601.0 0 12 52 6 601.0 0 6 53 4 601.0 0 4 54 15 601.0 0 15 55 11 601.0 0 11 56 9 601.0 0 9 57 6 601.0 0 6 58 9 601.0 0 9 59 14 601.0 0 14 60 19 601.0 0 19 61 11 601.0 0 11 62 1 601.0 0 1 63 6 601.0 0 6 64 11 601.0 0 11 65 7 601.0 0 7 66 7 601.0 0 7 67 12 601.0 0 12 68 7 601.0 0 7 69 12 601.0 0 12 70 13 601.0 0 13 71 12 601.0 0 12 72 12 601.0 0 12 73 11 601.0 0 11 74 103 601.0 0 103 75 18 601.0 0 18 76 6 601.0 0 6 77 4 601.0 0 4 78 11 601.0 0 11 79 16 601.0 0 16 80 85 601.0 0 85 81 8 601.0 0 8 82 64 601.0 0 64 83 7 601.0 0 7 84 14 601.0 0 14 85 26 601.0 0 26 86 13 601.0 0 13 87 17 601.0 0 17 88 11 601.0 0 11 89 18 601.0 0 18 90 17 601.0 0 17 91 18 601.0 0 18 92 19 601.0 0 19 93 15 601.0 0 15 94 22 601.0 0 22 95 12 601.0 0 12 96 32 601.0 0 32 97 25 601.0 0 25 98 55 601.0 0 55 99 28 601.0 0 28 100 10 601.0 0 10 101 16 601.0 0 16 102 32 601.0 0 32 103 58 601.0 0 58 104 100 601.0 0 100 105 103 601.0 0 103 106 59 601.0 0 59 107 49 601.0 0 49 108 82 601.0 0 82 109 27 601.0 0 27 110 24 601.0 0 24 111 36 601.0 0 36 112 79 601.0 0 79 113 42 601.0 0 42 114 43 601.0 0 43 115 352 601.0 0 352 116 28 601.0 0 28 117 23 601.0 0 23 118 28 601.0 0 28 119 22 601.0 0 22 120 25 601.0 0 25 121 38 601.0 0 38 122 201 601.0 0 201 123 201 601.0 0 201 124 379 601.0 0 379 125 596 601.0 0 596 126 114 601.0 0 114 127 174 601.0 0 174 128 109 601.0 0 109 129 115 601.0 0 115 130 344 601.0 0 344 131 137 601.0 0 137 132 194 601.0 0 194 133 139 601.0 0 139 134 178 601.0 0 178 135 400 601.0 0 400 136 270 601.0 0 270 137 586 601.0 0 586 138 992 601.0 0 992 139 1134 601.0 0 1134 140 1988 601.0 0 1988 141 3125 601.0 0 3125 142 44942 601.0 0 44942 143 24514 601.0 0 24514 144 103295 601.0 0 103295 145 399 601.0 0 399 146 44 601.0 0 44 147 44 601.0 0 44 148 23 601.0 0 23 149 45 601.0 0 45 150 41 601.0 0 41 151 28 601.0 0 28 152 34 601.0 0 34 153 44 601.0 0 44 154 16 601.0 0 16 155 55 601.0 0 55 156 38 601.0 0 38 157 60 601.0 0 60 158 90 601.0 0 90 159 115 601.0 0 115 160 244 601.0 0 244 161 423 601.0 0 423 162 5404 601.0 0 5404 163 2598 601.0 0 2598 164 11179 601.0 0 11179 165 49 601.0 0 49 166 10 601.0 0 10 167 21 601.0 0 21 168 17 601.0 0 17 169 20 601.0 0 20 170 10 601.0 0 10 171 7 601.0 0 7 172 5 601.0 0 5 173 3 601.0 0 3 174 6 601.0 0 6 175 7 601.0 0 7 176 14 601.0 0 14 177 20 601.0 0 20 178 17 601.0 0 17 179 7 601.0 0 7 180 6 601.0 0 6 181 5 601.0 0 5 182 5 601.0 0 5 183 9 601.0 0 9 184 6 601.0 0 6 185 13 601.0 0 13 186 5 601.0 0 5 187 8 601.0 0 8 188 35 601.0 0 35 189 63 601.0 0 63 190 144 601.0 0 144 191 8 601.0 0 8 192 4 601.0 0 4 193 9 601.0 0 9 194 7 601.0 0 7 195 13 601.0 0 13 196 12 601.0 0 12 197 12 601.0 0 12 198 5 601.0 0 5 199 11 601.0 0 11 200 12 601.0 0 12 201 15 601.0 0 15 202 6 601.0 0 6 203 7 601.0 0 7 204 11 601.0 0 11 205 9 601.0 0 9 206 16 601.0 0 16 207 7 601.0 0 7 208 22 601.0 0 22 209 19 601.0 0 19 210 30 601.0 0 30 211 32 601.0 0 32 212 27 601.0 0 27 213 16 601.0 0 16 214 34 601.0 0 34 215 58 601.0 0 58 216 128 601.0 0 128 217 56 601.0 0 56 218 12 601.0 0 12 219 9 601.0 0 9 220 10 601.0 0 10 221 10 601.0 0 10 222 18 601.0 0 18 223 28 601.0 0 28 224 22 601.0 0 22 225 27 601.0 0 27 226 65 601.0 0 65 227 104 601.0 0 104 228 10 601.0 0 10 229 12 601.0 0 12 230 12 601.0 0 12 231 20 601.0 0 20 232 13 601.0 0 13 233 18 601.0 0 18 234 18 601.0 0 18 235 11 601.0 0 11 236 14 601.0 0 14 237 39 601.0 0 39 238 46 601.0 0 46 239 30 601.0 0 30 240 48 601.0 0 48 241 23 601.0 0 23 242 170 601.0 0 170 243 20 601.0 0 20 244 12 601.0 0 12 245 23 601.0 0 23 246 21 601.0 0 21 247 10 601.0 0 10 248 13 601.0 0 13 249 12 601.0 0 12 250 15 601.0 0 15 251 7 601.0 0 7 252 17 601.0 0 17 253 17 601.0 0 17 254 14 601.0 0 14 255 48 601.0 0 48 256 30 601.0 0 30 257 115 601.0 0 115 258 34 601.0 0 34 259 29 601.0 0 29 260 33 601.0 0 33 261 64 601.0 0 64 262 97 601.0 0 97 263 83 601.0 0 83 264 15 601.0 0 15 265 13 601.0 0 13 266 16 601.0 0 16 267 13 601.0 0 13 268 14 601.0 0 14 269 27 601.0 0 27 270 17 601.0 0 17 271 17 601.0 0 17 272 14 601.0 0 14 273 15 601.0 0 15 274 16 601.0 0 16 275 12 601.0 0 12 276 23 601.0 0 23 277 20 601.0 0 20 278 93 601.0 0 93 279 109 601.0 0 109 280 72 601.0 0 72 281 39 601.0 0 39 282 27 601.0 0 27 283 33 601.0 0 33 284 23 601.0 0 23 285 21 601.0 0 21 286 16 601.0 0 16 287 32 601.0 0 32 288 22 601.0 0 22 289 21 601.0 0 21 290 20 601.0 0 20 291 12 601.0 0 12 292 24 601.0 0 24 293 21 601.0 0 21 294 19 601.0 0 19 295 34 601.0 0 34 296 52 601.0 0 52 297 41 601.0 0 41 298 24 601.0 0 24 299 18 601.0 0 18 300 26 601.0 0 26 301 9 601.0 0 9 Finished in 10.04 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 282,370 Reads with adapters: 32,268 (11.4%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 32,244 (11.4%) Total basepairs processed: 54,380,685 bp Total written (filtered): 8,929,240 bp (16.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 32268 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 127 0.0 3 0 126 1 17 32019 0.0 3 31356 636 26 1 18 56 0.0 3 6 50 19 20 0.0 3 2 0 17 1 20 5 0.0 3 0 0 0 5 46 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 72 3 0.0 3 0 0 0 3 79 2 0.0 3 0 0 0 2 82 3 0.0 3 0 0 0 3 108 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 144 4 0.0 3 0 0 0 4 146 2 0.0 3 0 0 0 2 147 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 180 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 1 194 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.13 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 32,244 Reads with adapters: 81 (0.3%) Reads written (passing filters): 32,244 (100.0%) Total basepairs processed: 8,929,240 bp Total written (filtered): 8,928,991 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 81 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 9.9% C: 17.3% G: 14.8% T: 58.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 79 503.8 0 79 6 2 7.9 0 2